145results about How to "Realize on-site inspection" patented technology

The invention relates to the technical field of biology, and specifically provides a detection kit for a detection kit for antigens of a novel coronavirus (SARS-CoV-2). According to the detection kitprovided by the invention, the antigens of the SARS-CoV-2 are detected by virtue of a colloidal gold double antibody sandwich method, and two monoclonal antibodies and colloidal gold are mixed for labeling, so that a detection result of the antigens of the SARS-CoV-2 is accurate and high in sensitivity, and meanwhile, the detection rate can be remarkably increased. According to the detection kit,a blank in immunological detection of the SARS-CoV-2 is filled up, and field detection can be realized without a detection instrument, so that the time and the labor are saved, and operation is flexible.

The invention discloses a test strip for fluorescence immunochromatography of myeloperoxidase. The test strip comprises a base plate, a sample pad, a binding pad, a nitrocellulose membrane and an absorbent pad, and the sample pad, the binding pad, the nitrocellulose membrane and the absorbent pad are assembled on the base plate in a sequential overlapping manner, wherein the absorbent pad and thebinding pad are respectively pressed on two ends of the nitrocellulose membrane in an overlapping manner, and a detection area is formed on the surface of the nitrocellulose membrane; the sample pad is pressed on the binding pad in an overlapping manner, and a myeloperoxidase antibody-fluorescent microsphere compound is immobilized on the binding pad; and the nitrocellulose membrane in the detection area is coated with a detection line formed by a monoclonal antibody for recognizing another epitope of myeloperoxidase and a control line formed by a goat anti-mouse IgG polyclonal antibody. The test strip has the advantages of high sensitivity, high stability, realization of the detection linearity being 3.125-600 ng/ml, no non-specificity, short detection time of 5 min, realization of bedside quick test, and great improvement of the clinical diagnosis efficiency.

The invention discloses a probe, a primer, a kit, and a detection method for detecting novel coronavirus SARS-CoV-2, and application, belongs to the technical field of molecular biology nucleic acid detection technology, and aims to solve problems in field detection, reduce pollution caused in detection process, increase virus RNA detection sensitivity and specificity. The invention provides the probe and the primer for detecting novel coronavirus SARS-CoV-2, and the kit containing the probe and the primer. The invention further provides a whole-course encapsulated procedure for exponential amplification from RNA (WEPEAR method). According to the invention, the detection sensitivity on N gene as low as 1ag (about 4 copies) is realized; the detection sensitivity on S gene as low as 1 copy is achieved, meanwhile, double genes of virus nucleic acid RNA based on RT-RPA (or RT-ERA) are detected at the same time so as to further improve the reliability of the detection result, and the kit can be used for field detection or household detection.

The invention relates to a differential ion mobility spectrometry and high field asymmetric waveform ion mobility spectrometry combination device, which comprises a differential ion mobility spectrometry, a high field asymmetric waveform ion mobility spectrometry and an interface device connecting the differential ion mobility spectrometry and the high field asymmetric waveform ion mobility spectrometry, and is characterized in that the differential ion mobility spectrometry comprises a first substrate, a first gas inlet port, an ion source, an ion source substrate, a second gas inlet port, afirst tail gas port, a first ion selective electrode and a second ion selective electrode, the high field asymmetric waveform ion mobility spectrometry comprises a second substrate, a third gas inletport, a second tail gas port, a first ion separation electrode, a second ion separation electrode, a weak current detection electrode, a weak current detector and a bias electrode, and the interface device is composed of a first traction electrode and a second traction electrode. According to the technical scheme, the device realizes twice separation for differences in spatial position and migration speed of ions of a substance under test, and can realize field detection of the ion mobility spectrometry for pollutants in a complex environment.

The invention discloses a CyHV-2 (Cyprinid Herpesvirus 2) monoclonal antibody, a hybridoma cell and an application of the CyHV-2 monoclonal antibody. The CyHV-2 monoclonal antibody disclosed by the invention is secreted from a hybridoma cell GFHNV-6G7 with the collection number of CCTCC No. C2013114 or hybridoma cell GFHNV-3B5 with the collection number of CCTCC No. C2013115. The CyHV-2 monoclonal antibody disclosed by the invention has good specificity and potency; by producing rapid immunodetection test paper from the CyHV-2 monoclonal antibody, the speed and efficiency of detection can be increased, and field detection on CyHV-2 can be realized. The CyHV-2 monoclonal antibody disclosed by the invention makes up the blank in immunodetection on the CyHV-2 and lays a foundation for immunological research on the CyHV-2.

This invention discloses one spring steel carbon layer removing depth test method, which belongs to thermal process carbon layer depth lossless test technique field, wherein it uses spring steel part for quenching and annealing organization change to rouse size change to test parts diameter before and after thermal process; its processes the test in the thermal process according to its diameter difference values.

The invention discloses a portable cholesterol molecular imprinting silk-screen printing electrochemical tester and belongs to the technical field of rapid detection. A silk-screen printing electrode is polymerized with template molecule cholesterol by electroplating and a mixed solution of a purified sample and conductive liquid is dropwise added on the surface of a working electrode of the silk-screen printing electrode; current of cholesterol can be directly read by the portable cholesterol molecular imprinting silk-screen printing electrochemical tester; a standard curve is corrected according to a silk-screen printing electrode piece with the cholesterol with the standard concentration; and the concentration of the cholesterol in the detected sample is calculated by a built-in program. The tester disclosed by the invention has a simple structure, is portable, sensitive, rapid and accurate, has high specificity and is economical. The tester can be used for directly detecting the cholesterol, has the advantages of portability, sensitivity, rapidness, high specificity and the like, has low price and is suitable for detecting the cholesterol on a base layer or in the field.

The invention discloses a method and a device for detecting the corrosion of a grounding grid of an electric transmission line iron tower. The device comprises a polarization resistance detection unit, a dielectric resistance detection unit, a Tafel constant detection unit, a detection result generating unit and a detection result output unit, wherein the polarization resistance detection unit is used for applying a linear polarization direct current signal to detected grounding grid metal and soil medium for measuring the polarization resistance of a corrosion system; the dielectric resistance detection unit is used for applying a high-frequency alternating current signal to the detected grounding grid metal and soil medium for measuring the dielectric resistance of the corrosion system; the Tafel constant detection unit is used for applying a weak polarization direct current signal to the detected grounding grid metal and soil medium for measuring the Tafel constant of the corrosion system; the detection result generating unit is used for calculating and generating the actual polarization resistance of the corrosion system according to the polarization resistance and the dielectric resistance and calculating and generating corrosion current detection data of the detected grounding grid metal according to the actual polarization resistance and the Tafel constant; and the detection result output unit is used for outputting detection result data including the corrosion current density data. The device is used for solving the problem of corrosion detection of the grounding grid of the electric transmission line iron tower.

The invention discloses a DNA methylation real-time detection method and an application thereof. Based on DNA double-stranded long-range electron transfer characteristic, a specific chemical derivative reaction is combined, a rapid and simple DNA methylation dynamic process real-time electrochemical detection method and an electrochemical biological sensor are established, which can be used for real-time detection of a DNA methylation process, enzyme inhibitor screening and enzyme sequence preference research.

The invention relates to a method for detecting ractopamine content in food. The method is characterized by comprising the following steps: preparing a capture probe; preparing a signal probe, treating a sample to be detected, and detecting by using a glucometer. Compared with the prior art, the method has the advantages that the signal is converted into a glucose signal which can be detected by the glucometer through an enzymatic effect, field detection of a nonsugar glucometer is realized, the stability is high, the detection limit is low, the detection range is wide. Moreover, the detection cost is low, the detection program is simple, the detection effect is good, particularly general staff can perform field detection, and the strict requirement that the professional staff need to operate in a lab in the prior art is avoided.

The invention discloses the production method of immune sensing electrode for detecting chloramphenicol residue, involving detection technology field; its characteristic is that the said immune sensing electrode is the nano-modified printing electrode affixed with chloramphenicol antibody fixed sensing membrane, and the production method of the said immune sensing electrode as follows: 1) chloramphenicol monoclonal antibody preparation; 2) chloramphenicol antibody fixed membrane preparation; 3) drug enzyme label preparation; 4) screen printing electrode production; 5) nano-modified electrode production; 6) immune sensing electrode production; 7) finally, connecting electric signal output, display and data processing, transformation electronic components system, and when measuring, it can directly display the content or concentration value of measured remnants; using the immune sensing electrode biosensor, the detection is accurate, sensitive, inexpensive, rapid response and detection, good selectivity, simple operation, easy to carry, repeatedly used, and capable of in field detection and on-line detection of chloramphenicol residues.

The invention discloses a haemonchus contortus nucleic acid detecting primer pair and probe. Nucleotide sequences of upstream and downstream primers of the primer pair are shown as a sequence 1 and asequence 2 in a sequence table, and a nucleotide sequence of the probe is shown as a sequence 3 in the sequence table. The invention further discloses a rapid detection kit and a detection method. Thehaemonchus contortus nucleic acid detecting primer pair and probe and the rapid detection kit are established on the basis of molecular biology, the primer pair and the probe are capable of realizingspecific amplification of haemonchus contortus, the rapid detection kit for rapid haemonchus contortus nucleic acid detection is higher in sensitivity than PCR (polymerase chain reaction) and is highin specificity and detection speed, and high simplicity and convenience in result determination operation are achieved. By combination of the detection method, the detection kit and an existing nucleic acid rapid extraction method, farm onsite detection in a non-lab environment can be realized, and accordingly measures can be taken timely to clear haemonchus contortus.

The invention discloses a primer set, probe kit and detection method for quickly detecting an African swine fever virus based on a RPA method. The primer set and the probe comprise a primer set and aprobe, wherein the primer set comprises a forward primer RPA-F and a reverse primer RPA-R; the total amplification product size of the primer set and the probe is 267bp; and the kit comprises the primer set and the probe. The detection method comprises the following steps of synthesizing the primer set and the probe; and establishing an RPA-LFD detection method. According to the primer set, the probe, the kit and the detection method disclosed by the invention, complex and refined instruments and equipment are not needed, so that the fund investment is reduced; the implement process is simpleand easy to operate, and on-site detection can be performed without specialized detection staff; and the detection is quick and accurate, so that on-site visualized detection of African swine fever can be realized, and the primer set, the probe, the kit and the detection method have important significance to prevention and control of the African swine fever virus.

A verticality symmetry inspection tool of a differential shell belongs to the technical field of automobile machining. The verticality symmetry inspection tool comprises a mandrel and a positioning mandrel, wherein the center of the positioning mandrel is provided with a positioning hole, and the mandrel is plugged into the positioning hole and connected with the positioning mandrel. Compared with the prior art, the verticality symmetry inspection tool has the beneficial effects that in-situ inspection can be realized, the inspection process is simple, the inspection precision is high, the inspection cost is low, and convenience in inspection is realized.

The invention relates to an intermediate shaft spiral gear meshing position degree gauge. The intermediate shaft spiral gear meshing position degree gauge includes an auxiliary measurement device anda base; two support blocks for supporting an intermediate shaft are fixed to the base and are located at the two shaft ends of the intermediate shaft respectively to locate the shaft end excircles andend faces of the intermediate shaft, a support is fixed to the portion, located at the side of the intermediate shaft, of the base, and a first bolt for locating a first gear on the intermediate shaft and a second bolt for locating a second gear on the intermediate shaft are mounted on the support; the auxiliary measurement device is fixed to the intermediate shaft, rotates along with rotation ofthe intermediate shaft and is provided with a scale line marking face extending in the radial direction of the intermediate shaft. The intermediate shaft spiral gear meshing position degree gauge candirectly detect the position degree at a production site during machining, the procedure and quality are convenient to control, and unnecessary carrying links can also be omitted.

The invention discloses a melamine selective electrode and a preparation method thereof, and the electrode comprises a substrate electrode which is coated with a poly-o-toluidine (POT) membrane on the surface, a polymethyl methacrylate-dimethylaminoetyl methacrylate (PMMA-PDMA) sensitive membrane with an electroactive substance, a glass cannula, epoxy resin, and a wire. The melamine selective electrode provided by the invention has an all-solid-state form, uses a PMMA-PDMA copolymer membrane without any plasticizer to overcome the disadvantages of a traditional PVC membrane of difficult adhesion, easy leakage, poor thermal stability, and the like, uses a POT membrane to inhibit the water layer formation between the sensitive membrane and the substrate electrode, and has the advantages of being stable in measuring voltage, low in detection limit, simple in structure, convenient to use, capable for miniaturization, etc. The detection of melamine in milk and dairy products by using the melamine selective electrode provided by the invention as an indicator electrode and adopting a potential analysis method has high sensitivity, good selectivity, quick detection speed, simple equipmentrequired, low analysis cost, and can realize on-site detection.

The present invention relates to chemical analysis of soil, especially relates to a method for measuring quick-acting phosphorus in soil based on image analysis. The phosphorus -containing liquid to be measured is reacted with a color reagent and the blue color is displayed. The darkness of blue can be characterized by the blue degree which is highly dependent linearly with the phosphorus content. The image averaging blue degrees of the soil liquid to be measured and the sample are obtained with the image analysis software. A linear equation between the concentration of sample and the average blue degree value of the sample is established Y=a-bX (wherein Y is the concentration of sample, X is the average blue degree value of sample, a and b are constant quantities). The average blue degree value of the liquid to be measured is substituted into the equation to obtain the phosphor concentration of the phosphorus-containing liquid to be measured. Then the content of quick-acting phosphorus in soil is calculated. The precision of the invention is higher than that of the test paper method. The operation of the invention is simpler than that of the colorimetry. Furthermore the method of the invention is suitable for the high-concentration quick-acting phosphorus in soil.