Method for detecting ractopamine content in food

A technology of ractopamine and detection method, which is applied in the direction of food testing, biological testing, material inspection, etc., to achieve strong recognition and enrichment functions, improve solubility and stability, and low price

Active Publication Date: 2014-05-14
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing ractopamine is no longer suitable for this shift in detection direction

Method used

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  • Method for detecting ractopamine content in food
  • Method for detecting ractopamine content in food
  • Method for detecting ractopamine content in food

Examples

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Embodiment 1

[0041] Detection of ractopamine content in pork

[0042] Different pork and liver samples were purchased from local supermarkets and processed separately according to conventional methods. The processing method is briefly described as follows:

[0043] After crushing the sample, take 1.0 gram and evenly disperse it in 2 mL of 3% trichloroacetic acid, vortex with a vortexer until it is uniform, then add 1 mL of acetonitrile and shake well. Subsequently, it was centrifuged at 4000 rpm for 10 minutes, and the supernatant was collected. After that, the collected supernatant was adjusted to a pH of 9.8±0.2 with 10 wt% sodium carbonate, and then 0.8 g of sodium chloride was added. Subsequently, 2 mL of ethyl acetate was thoroughly shaken, mixed and extracted twice, and centrifuged at 4000 rpm for 10 minutes. Take the upper liquid and blow dry at 50-60°C under nitrogen flow, add 1ml PBS buffer solution and shake it well for detection.

[0044] In this example, ten pork samples were prep...

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Abstract

The invention relates to a method for detecting ractopamine content in food. The method is characterized by comprising the following steps: preparing a capture probe; preparing a signal probe, treating a sample to be detected, and detecting by using a glucometer. Compared with the prior art, the method has the advantages that the signal is converted into a glucose signal which can be detected by the glucometer through an enzymatic effect, field detection of a nonsugar glucometer is realized, the stability is high, the detection limit is low, the detection range is wide. Moreover, the detection cost is low, the detection program is simple, the detection effect is good, particularly general staff can perform field detection, and the strict requirement that the professional staff need to operate in a lab in the prior art is avoided.

Description

Technical field [0001] The invention relates to a detection and analysis method for substances, and specifically refers to a detection method for ractopamine content in food. Background technique [0002] Ractopamine is a β-stimulant, which can transfer the nutrients in the animal body from fat to muscle, showing the effect of nutrient redistribution, thereby regulating the material metabolism of the animal body, enhancing the decomposition of fat, promoting protein synthesis, and significantly increasing the lean carcass rate And feed remuneration. However, their abuse and residues in animal food seriously endanger people's health and life safety, and seriously affect the export trade of my country's livestock and poultry products. Most countries in the world expressly prohibit ractopamine as a feed additive. [0003] For the detection of ractopamine, more mature methods and technologies, such as mass spectrometry, chromatography, spectroscopy, and enzyme-linked immunosorbent as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/02G01N33/53
Inventor 干宁陈思曹玉廷胡富陶李天华崔焕张佳斌王德熊萍
Owner NINGBO UNIV
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