Detection for zearalenone

A technology for zearalenone and a detection method, which is applied in the directions of measuring devices, biological tests, material inspection products, etc., can solve the problems of complicated operation, inability to popularize and popularize, pollute the environment, etc., and achieve the effect of simple operation.

Inactive Publication Date: 2005-07-27
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although thin-layer chromatography is simple and economical, it has many steps and low sensitivity
Gas chromatography and high performance liquid chromatography have high sensitivity, but the sample treatment is cumbersome, the operation is complicated, and the equipment is expensive
In addition, these determination methods need to use highly toxic ZEN as a standard substance during operation, and toxic and odorous organic solvents are required for pretreatment. These substances not only poison operators, but also pollute the environment
The above methods are only suitable for testing in the laboratory, and cannot be promoted and popularized. Therefore, most enterprises do not carry out ZEN supervision and testing at all, and have been in a state of laissez-faire.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation and preparation of immunoaffinity column:

[0028] 1. Preparation of monoclonal antibodies. The preparation of monoclonal antibodies is obtained through steps such as mouse immunization, cell fusion, hybridoma screening, obtaining hybridoma cell lines, and collecting monoclonal antibodies. The steps are:

[0029] (1) Antigen synthesis: a. Preparation of zearalenone-carboxymethoxim: Weigh 18 mg of zearalenone standard substance and 45 mg of carbonylmethoxamine and place it in a brown vial, add 0.6 ml of pyridine to dissolve it, After stirring at room temperature for 24 hours, dry it with a vacuum pump, then dissolve it with 5ml of distilled water, adjust the pH to 9.0 with NaOH, and continuously extract 3 times with 4ml of dichloromethane, then adjust the pH of the upper aqueous solution to 2.0 with HCL, and stand still for 5 minutes Continuously extract 3 times with 4ml of dichloromethane, combine and collect the dichloromethane liquid for 3 times, dehydr...

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Abstract

A detection method includes preparing single cloning antibody by steps of antigen synthesis, mouse immune, cell fusion, hybrid tumor selection and its cell line collection, extracting ZEN in corn sample by methanol / aqueous solution; filtering and deluting it; flowing liquid sample through affinity column and it by methanol; and using fluoresclence spectrophotometer to measure out content of zearlenone in set sample solution.

Description

technical field [0001] The invention relates to a detection method of zearalenone, which is a method for detecting zearalenone by using an immunoaffinity column prepared by a zearalenone monoclonal antibody and a fluorescence photometer, belonging to biological cytology and The technical field of detection methods for toxic metabolites of microbial fungi. Background technique [0002] Zearalenone (zearalenone, ZEN) is a toxic metabolite produced by Fusarium graminearum, Fusarium three-line, Fusarium oxysporum, Fusarium yellow, Fusarium moniliforme, Fusarium oats, Fusarium equisete, etc. , is a class of estrogenic mycotoxins, also known as F2 toxins. Zearalenone mainly exists in corn and corn products, and also has a certain degree of distribution in wheat, barley, sorghum and rice. [0003] Zearalenone has strong reproductive toxicity and teratogenic effect, can cause hyperestrogenism in animals, lead to infertility or abortion in animals, and has a greater impact on poult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/88G01N33/52G01N33/53G01N33/531
Inventor 陈宇光黎双华顾鸣
Owner SHANGHAI UNIV
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