Method for making immune sensing electrode for detection of residual chloromycetin

Abstract

The invention discloses the production method of immune sensing electrode for detecting chloramphenicol residue, involving detection technology field; its characteristic is that the said immune sensing electrode is the nano-modified printing electrode affixed with chloramphenicol antibody fixed sensing membrane, and the production method of the said immune sensing electrode as follows: 1) chloramphenicol monoclonal antibody preparation; 2) chloramphenicol antibody fixed membrane preparation; 3) drug enzyme label preparation; 4) screen printing electrode production; 5) nano-modified electrode production; 6) immune sensing electrode production; 7) finally, connecting electric signal output, display and data processing, transformation electronic components system, and when measuring, it can directly display the content or concentration value of measured remnants; using the immune sensing electrode biosensor, the detection is accurate, sensitive, inexpensive, rapid response and detection, good selectivity, simple operation, easy to carry, repeatedly used, and capable of in field detection and on-line detection of chloramphenicol residues.

Description

technical field [0001] The invention relates to a detection technology, in particular to a biological immunity test technology for rapidly detecting chloramphenicol residue in animal food. Background technique [0002] Milk contains all the nutrients for human growth and health, and is deeply loved by the masses. For example, the annual per capita consumption of milk in Shanghai has reached 25 kilograms. However, the primary problem in the safety of milk is the residue of antibiotics in milk. Chloramphenicol is an antibiotic often used in the breeding industry. Farmers rarely sell milk in accordance with national standards, which poses a great food safety hazard to consumers. At the same time, taking antibiotics for a long time will cause bacterial resistance. The current detection method for chloramphenicol is very cumbersome, time-consuming, and the detection cost is very high. At the same time, it also requires expensive instruments and is difficult to promote. [0003...

AI Technical Summary

Problems solved by technology

[0007] After searching the existing patent technical literature, it is found that the patent application number is CN02137940.8, a chloramphenicol enzyme immunoassay kit and its detection method in animal food. The chloramphenicol residues are detected, and 10 kinds of reagents are involved in the detection process, and the use process is relatively cumbersome

In addition, the ELISA method has problems such as high false positives and false negatives, and poor repeatability. During the on-site detection process, other instruments are required to read the plate, which brings some troubles to the rapid detection.

At the same time, by searching other relevant information, it is found that the detection of chlorase is still more dependent on liquid phase and ELISA methods, and there is no report on the use of immunosensors for detection; there is an urgent need to develop a simple, fast, efficient, accurate, miniature , Portable, testing instruments and tools suitable for on-site and online testing

Images