95 results about "Biological immunity" patented technology

The present invention relates to identifying protein epitopes and more particularly to a novel method for identifying, determining the location, optimal length of amino acid residues and immunobiological potency of protein epitopes by applying a custom negative cosine function fit algorithm to a protein hydropathy scale. This fit analysis is supplemented with experimental immunobiological data. The amino acid sequence of the protein epitopes of the present invention exhibit a hydrophobic-hydrophilic-hydrophobic hydropathy pattern of an approximately fixed length in a given protein.

The present invention relates to identifying protein epitopes and more particularly to a novel method for identifying, determining the location, optimal length of amino acid residues and immunobiological potency of protein epitopes by fitting a hydrophilicity and / or hydrophobicity plot generated for the amino acid linear sequence of a polypeptide to a mathematically generated continuous curve thereby generating at least one set of potential epitopes which include ranked potential epitopes having a specific number of amino acid residues. The immunobiologically-active linear peptides are deemed the potential epitopes that exhibit the most alternating positioning about an equilibrium position when juxtaposed on the hydrophilicity and / or hydrophobicity plot and their optimal length corresponds to the specific number of amino acid residues in the set of ranked potential epitopes. The amino acid sequence of the protein epitopes of the present invention exhibit a hydrophobic-hydrophilic-hydrophobic motif

The invention provides an up-converting phosphor (UCP) bio-detector and a method for detecting sampling test strips. The UCP bio-detector consists of an optical system, a photo-detector, a control system, a driving module, a stepping motor and a scanning platform for placing the test strips, wherein, the control system comprises a micro-processor, a photo-detector interface, a signal acquisition circuit, a driving module interface, an LCD (liquid crystal display) module, a matrix keyboard, an RFID (radio frequency identification) module, a D / A (digital to analog) converter, a memorizer, an SD (secure digital) memory card and a micro-printer; and each test strip consists of test paper mounted inside the casing of the test strip and an RFID card, the test paper comprises a sample pad, a detection band and a quality control band, and the RFID card contains the information including test paper ID, substances to be detected, decision values, a parameter A and a parameter B. The control system of the invention has the advantages of simple design and convenient debugging, and the biological immunological detection can be carried out rapidly and accurately.

The invention relates to a dipterex bionic enzyme linked immunosorbent detection method. The bionic enzyme linked immunosorbent detection method with high sensitivity on the dipterex is established by using a dipterex molecular imprinting polymer film as an artificial antibody instead of a biological antibody and by utilizing an enzyme linked immunosorbent assay principle. The lowest detection limit of the method is 7 ugL<-1> and is far less than the highest residue limit value; and the analysis time is greatly shortened (by 1.0 hour compared with the traditional biological immune analysis time), so the method is applicable to rapid detection of the dipterex. A bionic antibody prepared by the method can be reused for more than 50 times, so cost is greatly reduced; and the method is simplein pretreatment, high in sensitivity, simple in test operation and applicable to rapid detection of the dipterex in various kinds of food.

The invention belongs to the field of immunobiology, and relates to antigen epitope for preventing and treating trichinosis, a composition thereof and application thereof. According to the antigen epitope, an amino acid sequence is shown as any one of SEQ ID NO: 1-4. The antigen epitope has the effective trichina reduction rate and has the potential of being used as medicines (such as vaccines) for preventing and treating trichinization or diseases caused by the trichinization.

The invention belongs to the technical field of immunobiology and particularly relates to application for a patent of a PRV (pesudorabies virus) gB monoclonal antibody and application thereof. The antibody is obtained by screening PRV variant strains HeNZK-2014 and PRV gB proteins with hybridoma cells. The hybridoma cells capable of stably secreting the PRV gB protein monoclonal antibody is obtained through screening, so that the gB protein monoclonal antibody with high potency is obtained, a finished kit is prepared from the antibody, and a matched one-step competitive ELISA detection method is established. Through a series of verification for specificity, sensitivity and repeatability, the result indicates that according to the PRV gB antibody provided in the application and the established one-step competitive ELISA detection method, a comparatively accurate and comparatively stable detection result is achieved, the result can be better used for detection and evaluation of the PRV gB antibody, and the foundation is laid for preventing and controlling a PRV.

The present invention discloses in-situ transplantation process of constructing human liver cancer mouse model. The process includes using Kunming sucking mouse as the tumor carrying acceptor, and injecting the mouse liver lobes with human liver cancer cell suspension to in-situ transplanted tumor carrying model of human liver cancer cell. The process is simple and high in successful rate. The process makes it possible to monitor the mutual effect between liver cancer cell and mouse immune system under normal mouse body immune environment, simulate the whole process of immunological tolerance and antitumor immunity in human body and provide ideal operation platform for the immunobiological medicine screening and pharmacological research.

The invention belongs to the immunobiology and biological medicine fields, and relates to a polypeptide for enclosing a TGF-beta acceptor or an IL-10 acceptor, a pharmaceutical composition and an application. Concretely, the invention relates to the polypeptide or a polypeptide combination, an amino acid sequence is one or more shown as the following (1)-(4), 1) the amino acid sequence shown in a SEQ ID NO: 6, 2) the amino acid sequence shown in a SEQ ID NO: 14, 3) the amino acid sequence shown in a SEQ ID NO: 25, and 4) the amino acid sequence shown in a SEQ ID NO: 37. The polypeptide can effectively enclose the TGF-beta acceptor or the IL-10 acceptor, thereby the tumor killing activity of the T cell (especially the antigenic specificity T cell) is enhanced, and the polypeptide has potential as a medicine for treating or assistantly treating prostate.

The invention belongs to the technical field of biological immunity, and particularly relates to a hybridoma cell strain capable of stably secreting a monoclonal antibody against novel coronavirus nucleocapsid protein as well as an establishment method and application of the hybridoma cell strain. The hybridoma cell strain nCoVN3G6 capable of stably secreting the anti-SARS-CoV-2 monoclonal antibody is obtained by expressing and purifying a recombinant SARS-COV-2 virus N protein in escherichia coli and performing animal immunization by adopting the purified recombinant nucleoprotein. The nCoVN3G6 monoclonal antibody is utilized to establish a kit for detecting novel coronavirus antigens through a novel coronavirus capture method IgM ELISA, a kit for detecting the novel coronavirus antigens through a double-antibody sandwich method ELISA, a kit for detecting the novel coronavirus antigens through colloidal gold and a kit for detecting the novel coronavirus antigens through time resolution fluorescence chromatography, and a foundation is laid for diagnosis of SARS-COV-2 virus infection and research of infection mechanisms and vaccine development in the future.

The invention discloses a group of proteins with allergen activity from metupcllu ensis, a preparation technology thereof and application thereof in medicine, and belongs to the field of natural bioactive substances and preparation technologies and medical applications thereof. The invention provides a group of proteins with allergen activity in a tissue of the metupcllu ensis, and molecular weights of the proteins are 21.6KDa, 36KDa, 46.2KDa, 60KDa, 64.8KDa, 68KDa, 78KDa and 88.5KDa, respectively. The operation according to the invention can obtain main allergen protein compositions of the metupcllu ensis which have high-purity and maintain good immunobiologic activity. The main allergen protein compositions of the metupcllu ensis produced by the invention can be used to prepare diagnostic reagents and desensitizing preparations for clinical shrimp-induced anaphylaxis and prepare reagents used to detect shrimp food allergen.

The invention discloses a preparation method of a biological immunosensor which has an electrochemiluminescence and photoelectrochemistry dual signal developed strategy and is constructed on the basis of a functional nano material.The prepared immunosensor is easy to operate, convenient to carry, rapid in detection, low in cost and capable of being used for rapid and sensitive detection on parathion-methyl in the fields of daily production and life and the like.

The invention provides a biological probe and a biological immunosensor for detecting bungarus multicinctus venom and a method for detecting coral snake venom based on proportional signals, and belongs to the technical field of biological immunosensing. AuNPs / COF serves as a signal amplification substance, the probe has a large number of catalytic active sites, and has a catalytic reduction effect and an enrichment effect on a guest signal molecule p-nitrophenol when the probe is used for detection by an ELISA (enzyme-linked immunosorbent assay), and a proportional chemical biological immunosensor constructed by the probe can realize accurate measurement of bungarus multicinctus venom, has the advantages of good stability, good repeatability, wide detection range, short detection time and low cost. According to the biological immunosensor, S-GQD serves as a reducing agent, the stability of the sensor can be effectively improved, meanwhile, the reducing capacity of the sensor can be catalyzed and improved by the gold nanoparticles, the S-GQD and the AuNPs / COF have the synergistic positive feedback amplification effect on signals, and therefore the sensitivity of the sensor is improved.

A clonazepam colloidal gold method test paper and a preparation method belong to the testing technology field of the biological immunity method. The test paper is composed of a sample pad, a combined release pad, a cellulose nitrate membrane, a water absorbing pad and a plastic base plate; the sample pad, the combined release pad, the cellulose nitrate membrane and the water absorbing pad are sequentially stuck to the plastic base plate; the combined release pad is coated with an anti-clonazepam multi-resistance colloidal gold label; the cellulose nitrate membrane is sequentially coated with clonazepam coupling antigen and goat anti-rabbit IgG antibody, wherein the clonazepam coupling antigen is taken as the test line and the goat anti-rabbit IgG antibody is taken as the quality control line. The clonazepam colloidal gold method test paper and the preparation method have the characteristics of convenient use, economy, rapidness, simple preparation and low cost.

The invention discloses a preparation method of a functional nanomaterial based biological immunosensor with dual signal sending strategies of electrochemiluminescence and photoelectrochemistry.The sensor prepared according to the method has the advantages of simplicity in operation, portability, quickness in detection and low in cost and can be used for rapid and sensitive detection of diethylstilbestrol in fields of daily production, daily life and the like.

The invention belongs to the field of immunobiology, relates to immunogenic polypeptide for an enterovirus 71 type VP1 antigen as well as a preparation method and application of immunogenic polypeptide, in particular to immunogenic polypeptide for the enterovirus 71 type VP1 antigen, which has an amino acid sequence shown in any of sequence tables SEQ ID No.1-SEQ ID No.5 or has an amino acid sequence which has the same function and formed by replacing, deleting or adding one or more amino acids. The invention further relates to the preparation method of immunogenic polypeptide and application of immunogenic polypeptide to preparation of diagnostic reagents for EV71 viruses. The immunogenic polypeptide has good antigenicity and immunogenicity and provides a research foundation for development of diagnostic reagents and research on vaccines of EV71 at an early stage.

The invention discloses a preparation method of a biological immunity sensor which is based on functional nano-material construction and has dual signal development strategies of electrogenerated chemiluminescence and photoelectrochemistry, and the prepared sensor is easy to operate, convenient to carry, fast in detection, low in cost and capable of being used for fast and sensitive detection of estradiol in the fields of routine production and life and the like.

The invention discloses an application of a metallic ion specific response based fluorescence detection method in immune detection. According to the invention, a traditional metallic ion fluorescence detection method and a metal nanoparticle-label immune technology are combined and applied to the immune detection, the problem of difficulty in connection of traditional fluorescent molecules and biological protein molecules is solved, detection signals are effectively amplified, and the problems caused by strict requirements of the enzyme-catalyzed reaction for experimental conditions are solved; magnetic beads are taken as carriers of antigens and antibodies in the immune detection, rapid separation of to-be-detected samples can be realized, the detection process is simpler, more convenient and more rapid, and accuracy and sensitivity of the detection are improved greatly; a traditional enzyme catalysis process is replaced with the chemical reaction, the fluorescence detection method is introduced into the biological immune detection, so that the reaction time is shortened, the system stability is improved, the detection cost is reduced, and the application can play an important role in immune analysis and detection and clinical research.

The invention invents a group of mitten crab-derived proteins with allergen activity and a preparation process thereof, and belongs to the technical field of natural bioactive substances and preparation technologies thereof. The invention provides a group of proteins with the allergen activity in a tissue of a mitten crab, and the molecular weights of the proteins are respectively 76KDa, 66KDa, 60KDa, 53KDa, 48KDa, 41KDa, 35KDa, 34KDa and 29KDa. High-purity mitten crab allergen protein components with better immunobiologic activity can be obtained on the premise of operation according to the preparation process. Main mitten crab allergen protein components prepared according to the preparation process can be used for preparing diagnostic reagents and desensitizers of clinical crab allergy and preparing reagents for detecting crab food allergens.

The invention relates to the technical field of kits, and discloses a microbial immunofluorescence color developing kit which comprises a kit body, a placing groove is formed in the center of the top end of the kit body, a plurality of evenly-distributed inserting grooves are formed in the left side and the right side of the lower surface in the placing groove, and clamping assemblies are arranged in the inserting grooves. An insertion plate is mounted in the insertion groove through the clamping assembly, a placement plate is fixedly connected to the top end of the insertion plate, a plurality of through holes which are uniformly distributed and penetrate through are formed in the placement plate, reagent tubes are inserted into the through holes, and a plurality of through mounting grooves II which are uniformly distributed and penetrate through are formed in the center of the lower surface in the placement groove. When the microbial immunofluorescence developing kit is used, the reagent tubes are convenient to take out, so that the kit is convenient to use, meanwhile, the kit is convenient to carry when the microbial immunofluorescence developing kit is used, and the kit is relatively high in overall portability and relatively good in practicability.

The invention relates to an oestrone derivative for detecting oestrone content in a biological sample and a preparation method of the oestrone derivative. The structural formula of the oestrone derivative is shown as a formula (I). Oestrone immunogens with high immunogenicity and corresponding antibodies are prepared in succession by using the oestrone derivative. The oestrone immunogen is high inspecificity and high in immunogenicity, and the anti-oestrone specific antibody is high in specificity and high in titer, is capable of well recognizing and binding oestrone, and does not have any cross reaction with common 92 chaff interferents. The invention further discloses an oestrone enzyme-labeled conjugate, and an oestrone homogeneous enzyme immunoassay detection reagent is prepared by utilizing the anti-oestrone specific antibody and the oestrone enzyme-labeled conjugate. The reagent is capable of conveniently, rapidly, and accurately measuring the oestrone content in the biologicalsample, multiple samples are determined on a full-automatic biochemical analyzer, high throughput and rapid determination of the oestrone is realized, the accuracy is high, the specificity is high, and the accuracy and detection efficiency are greatly improved compared with the previous detection.

The invention belongs to the technical field of genetic engineering and biological immunotherapy, and constructs a novel chimeric antigen receptor (CAR), and a costimulatory signal domain of the novel chimeric antigen receptor comprises a novel costimulatory molecule, namely a reverse Dectin-1 (RD-1) intracellular domain. CAR-T formed by combining the newly constructed chimeric antigen receptor with T cells can secrete cell factors at a higher level and has better anti-tumor activity.

The invention belongs to the field of biological immunology, and more particularly relates to a fully human monoclonal antibody for neutralizing enterovirus 71 (EV71) and application of the fully human monoclonal antibody. An amino acid sequence of a heavy chain variable region is shown as any one of SEQ ID NO:14-20, and an amino acid sequence of a light chain variable region is shown as any one of SEQ ID NO:21-26. The invention further provides a nucleic acid molecule encoding the antibody, an expression vector, application and a composition. Binding molecules can prevent the EV71 from infecting susceptible cells and are fully human derived, compared with other animal derived (such as rat-derived) anti-EV71 molecules, immunogenicity is low, affinity is good, the treating effect is good, and side effects are low.

For the first time individual (free from impurities of penta- and hexa-acetylated derivatives) di-, tri- and tetra-acetylated S-LPS of endotoxic bacteria and combinations thereof were obtained and their immunobiological, physical-chemical and chemical-pharmaceutical properties were studied.For the first time the principal possibility of their clinical application was directly demonstrated as vaccines and pharmaceutical compositions containing the modified S-LPS individual as monocomponent or combinations thereof as two and three component active substance, respectively.The modified S-LPS and combinations thereof have high safety profile and provide low pyrogenicity and high immunogenicity. Developed on their basis vaccines and pharmaceutical compositions demonstrate anti-shock activity, high efficiency and specificity, broad-spectrum action and also good chemical-pharmaceutical parameters.

The invention discloses a manufacturing method of a biological immunity sensor which is constructed on the basis of a functional nanometer material and has electrogenerated chemiluminescence and photoeletrochemistry double signal developing strategies.The manufactured sensor is easy to operate, convenient to carry, high in detection speed, low in cost and capable of being used for detecting furazolidone in the fields of daily production and life fast and sensitively.

The invention relates to the field of biological immune cell culture solution extraction, in particular to a biological immune cell culture solution extraction system. The system comprises a base, a culture solution storage box is fixedly connected to the middle of the top of the base, and a booster air pump is fixedly connected to the right side of the top of the culture solution storage box. Two clamping plates and an arc-shaped block are used in cooperation, when a filling bottle falls between the two clamping plates and the arc-shaped block and a rotating circular truncated cone rotates, the two clamping plates are opened towards the two sides under the action of a guide block, and due to the fact that the two clamping plates can elastically rotate, when the filling bottle completely makes contact with a sponge plate, under the elastic action of a reset spring, the two clamping plates and the arc-shaped block can wrap the filling bottle, so that the moving stability of the filling bottle is improved, it can be guaranteed that the filling bottle is always located at the position of a filling point when the filling bottle passes through the guide block every time, the stability of liquid filling is guaranteed, and liquid overflowing is avoided.

The invention belongs to the field of immunobiology and relates to immunogenic polypeptide of 71-type VP1 antigen of enterovirus, a preparation method and application thereof, and particularly relatesto the immunogenic polypeptide of the 71-type VP1 antigen of the enterovirus. The immunogenic polypeptide has an amino acid sequence shown as any sequence of SEQ ID NO.1-SEQ ID NO.5 in a sequence table, or an amino acid sequence formed by substituting, deleting or adding one or more amino acids for the sequence and with equal functions. The invention also relates to the preparation method of theimmunogenic polypeptide and the application to preparation of a diagnostic reagent for EV71 viruses. The immunogenic polypeptide has good antigenicity and immunogenicity and provides research basis for early development of the diagnostic reagent and vaccine research and development of EV71.

The invention discloses an apple seedling cultivation method, and belongs to the technical field of plantation of apples. An apple free from a bag is cultivated through the four steps of collecting pollen, preparing a pollen nutrient solution, breeding a rootstock and cultivating a seedling. The pollen nutrient solution is combined in use with a fruit brightening biological immune membrane after pollination, the traditional whole-process bagging technique is not used in the treatment, the collected pollen and other nutritional substances are used together to prepare the nutrient solution afterthe early treatment, so that the utilization efficiency of the pollen is improved on one side, and the cost for a paper bag is saved on the other side. The acre yield of the red Fuji apple reaches 7100-7116 kg per acre, and is increased by 18.3-18.6% compared with the acre yield of 6000 kg per acre in the conventional bagging, the apple is crispy and tasty, the bagging cost is reduced, about 825yuan is saved for each acre, the disease incidence is only 0.9-1.2%, the environment is not polluted, and the economic benefit is remarkable.

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of ethinyl estradiol in daily production, life and other fields.

The invention discloses an oxcarbazepine derivative, an immunogen, an anti-oxcarbazepine specific antibody as well as a preparation method and application of the oxcarbazepine specific antibody. Firstly, a novel oxcarbazepine derivative and recombinant human serum albumin obtained through genetic engineering modification are coupled to prepare an oxcarbazepine artificial antigen, then the oxcarbazepine artificial antigen is used for immunizing an experimental animal to obtain an anti-oxcarbazepine specific antibody, and ELISA detection shows that the specific antibody is high in specificity and sensitivity and can be used for preparing the oxcarbazepine specific antibody. An interference experiment shows that the specific antibody does not have any cross reaction with 100 common drugs; the anti-oxcarbazepine specific antibody is applied to preparation of an oxcarbazepine detection reagent, the oxcarbazepine detection reagent comprises an oxcarbazepine average phase enzyme immunoassay reagent and an oxcarbazepine latex enhanced immunoturbidimetry detection reagent, and the detection reagent can realize high-flux and rapid detection of oxcarbazepine on a full-automatic biochemical analyzer.