43 results about "Biological immunity" patented technology

The invention belongs to the field of biological immunity, and discloses a linear B cell epitope of African swine fever virus NP419L protein and application. The epitope sequence shows good immunoreactivity through ASFV positive pig serum recognition verification. The African swine fever positive serum can be identified by using the B cell epitope identified by the invention to carry out ELISA (Enzyme-Linked Immunosorbent Assay) experiment, and the sensitivity of the African swine fever positive serum is superior to that of NP419L protein full length and other truncated fragments. The epitope identified by the invention has high conservative property in reference ASFV strains in a plurality of popular regions in China, and has wide universality and adaptability. The kit can be widely applied to etiological diagnosis, serological diagnosis, immunological detection and disease prevention and treatment of African swine fever viruses, and can be applied to preparation of African swine fever vaccines, pathogenesis research and the like.

The present invention relates to a circular RNA for targeted inhibition of fish neural necrosis virus replication, as well as a preparation method and application thereof. The circular RNA is NNV-circRNA-1, whose nucleotide sequence is shown in SEQ ID NO:1. The circular RNA is prepared by cyclizing linear NNV-circRNA-1 with T4 RNA ligase 1 and then removing residual linear RNA in the reaction system using RNase R enzyme. The circular RNA prepared by the present invention has good stability and high purity. NNV-circRNA-1 has a strong binding ability with NNV-RdRp protein, can effectively inhibit the replication of NNV virus, and can be used in the preparation of vaccines, antiviral drugs and other biological immune products for fish neural necrosis virus in the field of aquaculture.

The application belongs to the field of biological medicine, and particularly relates to a pharmaceutical composition for treating inflammatory bowel disease and application of the pharmaceutical composition. The disclosed pharmaceutical composition comprises anti-TNF monoclonal antibody, phosphoketolase-producing bacteria or a pharmaceutically acceptable viable bacterial preparation or extract thereof. The application comprehensively utilizes multi-dimensional data analysis and experimental verification of'microbe-immune', and finds that the phosphoketolase-producing bacteria can regulate host immune metabolic microenvironment, actively stabilize blood drug concentration of the anti-TNF monoclonal antibody and reduce immunogenicity, so as to achieve the purposes of stabilizing blood drug concentration of the anti-TNF monoclonal antibody and delaying clearance, significantly improve clinical response rate and remission rate, and is particularly suitable for a patient population with primary non-response or secondary non-response to the anti-TNF monoclonal antibody, thereby providing an effective solution for solving this clinical difficulty.

The invention provides a method for realizing accurate regeneration and functional reconstruction of a gingival structure in combination with regeneration induction, relates to the field of traction gingival structure regeneration and functional reconstruction, and relates to 4D bionic scaffold design, a cell activation and directional regeneration system, a microorganism-immune regulation and control network and real-time monitoring and self-adaptive repair. The 4D bionic stent is designed into a shape memory hydrogel matrix, a 4D response mechanism and a bionic micro-gradient structure. The shape memory hydrogel matrix is specifically composed of materials; according to the material, a shape memory hydrogel matrix is prepared from 60% of PVA, 30% of gelatin and 10% of an elastin system, temperature triggering is carried out, a response mechanism is started when the oral temperature is lower than 37 DEG C, the crystal glue matrix is attached to the curved surface of the gingiva, and the shape memory hydrogel matrix and the curved surface of the gingiva are in contact. The shrinkage rate of the hydrogel matrix is 15%. The method has the advantage that the regeneration and functional reconstruction of the gingival structure can be accurately realized.

The invention discloses an anti-human OX40 inhibitory antibody or an antigen binding fragment thereof, a preparation method and application, and belongs to the technical field of biological immunity. The EC50 of the human-mouse chimeric antibody Y07-L2 and the EC50 of the combination of the humanized antibody Y07-C2 and the human OX40, which are prepared by the invention, are close to those of a targeted antibody drug KHK-4083 for inhibiting the OX40. A heavy chain variable region of the human-mouse chimeric antibody Y07-L2 is as shown in SEQ ID NO.14, and a light chain variable region of the human-mouse chimeric antibody Y07-L2 is as shown in SEQ ID NO.15; the heavy chain variable region of the humanized antibody Y07-C2 is as shown in SEQ ID NO.20, and the light chain variable region of the humanized antibody Y07-C2 is as shown in SEQ ID NO.21; the CDR region of the humanized antibody Y07-C2 is mutated, and the mutant antibodies Y07-C2MH4 and Y07-C2MH4L3 are obviously superior to other antibodies.

The invention relates to preparation of a cyclic adenosine monophosphate (cAMP) detection kit by using a chemiluminescence immunoassay technology. Cyclic adenosine monophosphate is an important second messenger for cell signal transduction and has wide physiological functions. Cyclic adenosine monophosphate is small in molecular weight and poor in immunogenicity, so that when a corresponding antibody is prepared, a proper derivative site needs to be selected firstly, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic adenosine monophosphate hapten, preparation of a cyclic adenosine monophosphate complete antigen and an anti-cyclic adenosine monophosphate antibody, a method for measuring the concentration of cyclic adenosine monophosphate, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic adenylate derivative. The cyclic adenylic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention discloses a composition for improving the immunity of aquatic organisms, and particularly belongs to the field of functional feeds, and the composition for improving the immunity of aquatic organisms comprises the following components in parts by mass: 20-30 parts of sedum sarmentosum extract, 30-40 parts of chenopodium ambrosioides extract and 10-15 parts of perilla frutescens extract. The composition further comprises 4-6 parts by mass of astaxanthin and 7-9 parts by mass of vitamin C phosphate. In the composition provided by the invention, the sedum sarmentosum extract, the chenopodium ambrosioides extract and the perilla frutescens extract have a certain synergistic effect in the aspect of improving the immunity of tilapia mossambica, and after astaxanthin and vitamin C phosphate are added into the composition, the effect is better. The feed containing the composition for improving the immunity of aquatic organisms can significantly improve the non-specific immunity of tilapia mossambica.

The invention relates to the technical field of magnetic sensors, in particular to a non-diagonal giant magneto-impedance sensor for detecting biomarkers and a preparation method thereof.The non-diagonal giant magneto-impedance sensor comprises an impedance sensor, a PI film, a SiO2 film, a self-assembly monomolecular film and an array microcavity, and the impedance sensor comprises a sensitive element and an induction coil; a-aminopropyltriethoxysilane self-assembled monomolecular film prepared on a SiO2 film can be used for fixing a monoclonal antibody, a microcavity array is placed on the self-assembled monomolecular film and is used for biological immune reaction, the magnetic label adopts streptavidin magnetic beads, the surface of the magnetic bead is modified by streptavidin, and the magnetic label can be used for biological immune reaction. Streptavidin molecules having multiple peptide chains are shown, which can be used to specifically bind biotinylated antibodies. According to the impedance sensor, a thin strip with a parallel structure is adopted as a sensitive element, and high-sensitivity biomarker detection can be realized on the basis of the principle of a double-antibody sandwich method.

The present invention relates to a fish circular RNA with antibacterial infection function, its preparation method, and application. The circular RNA is circRNA-AntiB1, whose nucleotide sequence is shown in SEQ ID NO:1. The circular RNA is prepared by cyclizing linear circRNA-AntiB1 with T4 RNA ligase 1 and then removing the residual linear RNA in the reaction system using RNase R. The circRNA-AntiB1 prepared by the present invention can effectively inhibit Vibrio infection and can be used in the preparation of Vibrio vaccines, antibacterial drugs, and other biological immune products in the field of aquaculture. It has good stability and good market application prospects.

The invention relates to the technical field of electrochemical immunosensors, and provides an MXene (at) PANI (at) Au composite material and application thereof in detection of sepsis biomarkers. Through research and development of the MXene-coated PANI-coated Au composite material, the problems of high operation complexity, high cost, potential cross reaction, need of professional operation and need of detection in a specific place in the current detection process are solved. According to the MXene-coated PANI-coated Au biological immunosensor disclosed by the invention, the two electrodes can share the same sample, the same time and the same detection environment. The results of the two markers can be obtained at the same time through one-time sample adding. The method not only realizes fast, but also shortens the total detection time fundamentally, which has revolutionary significance for clinical scenes needing fast diagnosis.

The invention belongs to the technical field of biological immunity, and particularly relates to a monoclonal antibody for resisting pseudorabies virus gC protein and application of the monoclonal antibody. The affinity of the anti-PRV monoclonal antibody is greater than or equal to 5 * 10 < 9 > L / mol, the ELISA titer is greater than or equal to 1: 5.12 * 10 < 5 >, the IPMA titer is 1: 6.4 * 10 < 3 >, and the anti-PRV monoclonal antibody has the characteristic of high sensitivity. And the kit is suitable for various detection platforms such as ELISA, IPMA, Western Blot and the like, and a reliable tool is provided for rapid detection of the PRV antigen.

The present invention relates to the field of biological immunity, and discloses a method and system for analyzing and evaluating immunotoxicity, the method comprising: culturing immune cells and non-immune cells, constructing an immune microenvironment of immune cell tissue, and constructing an in vitro immune system model of the human body; inputting exogenous substances into the in vitro immune system model, and detecting the cellular immune parameters of the experimental in vitro immune system model; extracting the mouse genome of mice, constructing the gRNA-Cas9 complex of the target immune gene, implanting the gRNA-Cas9 complex into a preset mouse fertilized egg, cultivating the target gene fertilized egg into a target gene mouse, inputting exogenous substances into the body of the target gene mouse, and analyzing the humoral immune parameters of the blood sample; detecting the organ parameters of the immune organ, calculating the organ coefficient of the immune organ, and analyzing the immune organ status of the immune organ; analyzing the immunotoxicity evaluation report of the exogenous substance on the human immune system. The present invention can improve the accuracy of immunotoxicity analysis.

The invention discloses a multifunctional intravascular stent for diabetics and a preparation method of the multifunctional intravascular stent, and relates to the technical field of cardiovascular interventional therapy instruments. The polycitrate is used as a carrier on the surface of the intravascular stent body to form the drug-loaded slow-release coating, and the drug-loaded slow-release coating has high antioxidant content, can provide oxidative stress attenuation continuously for a long time, provides a good microenvironment for cell tissue recovery, and promotes regeneration and repair of blood vessels. The biological immunosuppressor and the diabetes specific drug are loaded in the drug-loaded slow-release coating, the biological immunosuppressor is a drug having an excellent effect on common patients, the diabetes specific drug is a drug having a specific effect on diabetic patients, and the two drugs have a synergistic effect, so that the effect of treating diabetes is achieved; the coating on the surface of the intravascular stent has the effects of resisting inflammation, improving endothelial dysfunction and inhibiting proliferation and migration of smooth muscle cells aiming at diabetic patients so as to promote regeneration and repair of diseased blood vessels of the diabetic patients.

The invention relates to preparation of a Uric acid detection kit by using a chemiluminescence immunoassay technology. Uric acid is small in molecular weight, poor in immunogenicity and many in-vivo analogues, so that an antibody prepared from a uric acid derivative designed by the uric acid is poor in specificity and is difficult to meet reagent detection requirements. According to the present invention, allantoin is innovatively used to prepare the hapten and the antibody, in the detection process, the uric acid in the sample only needs to be converted into the allantoin through the specific sample treatment liquid, and the content of the uric acid in the body is indirectly reflected by determining the concentration of the allantoin in the sample; the invention mainly relates to design and synthesis of an allantoin hapten, preparation of an allantoin complete antigen and an anti-allantoin antibody, a method for measuring the concentration of uric acid, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of an allantoin derivative. The allantoin derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention relates to a Trop2 antibody or an antigen binding fragment thereof and application thereof, and belongs to the technical field of biological immunotherapy, the antibody has good safety and a Trop2-targeting treatment effect, can be used for preparing a Trop2-targeting immune effector cell, and provides a treatment or improvement approach for diseases related to Trop2 expression.

This invention discloses a monoclonal antibody against RSV NS2 protein and its applications, relating to the field of bioimmunotechnology. The monoclonal antibody comprises the following amino acid sequences: CDR-H1 (SEQ ID NO. 7), CDR-H2 (SEQ ID NO. 9), CDR-H3 (SEQ ID NO. 11), CDR-L1 (SEQ ID NO. 14), CDR-L2 (LVS), and CDR-L3 (SEQ ID NO. 17). This monoclonal antibody can specifically bind to the RSV NS2 protein and can be applied to various detection methods for RSV NS2 protein, providing an important research tool for studying RSV infection mechanisms and establishing related detection technologies.

The present invention discloses a monoclonal antibody against duck reovirus σC protein and its hybridoma cell line and application, belonging to the technical field of biological immunology. The present invention immunizes BALB / c mice with recombinant immune σC protein of purified duck reovirus DRV-TH11 strain, and screens hybridoma cells that can stably secrete duck reovirus σC protein monoclonal antibodies through cell fusion technology to obtain duck reovirus σC protein monoclonal antibodies; by sequencing the hybridoma cells, the light chain and heavy chain sequences of the duck reovirus σC protein monoclonal antibodies are obtained, the amino acid sequence of the heavy chain variable region is shown in SEQ ID NO.2, and the amino acid sequence of the light chain variable region is shown in SEQ ID NO.4. The antibody prepared by the present invention has high specificity and stability, can specifically identify cells infected with duck reovirus, and is suitable for immunological detection.

The invention relates to a bionic immune dynamic defense algorithm applied to the aspect of text map privacy protection. Aiming at the limitations of the existing generative model in privacy protection and against attack defense, the invention realizes active and adaptive protection of privacy risks by simulating a biological immune mechanism, and mainly comprises the following steps of: processing cue words by using an epigenetic coding technology to enhance the privacy protection capability of text embedding; identifying and evaluating a potential privacy risk through a privacy detection unit, and dynamically disturbing a potential space of the text graph model according to a risk level; the immune memory module is used for storing and recalling a historical threat mode so as to improve the response efficiency to repeated attacks; and when the risk is continuously higher than a preset threshold value, triggering a component operation mechanism, and selectively adjusting or disabling the model component to quickly block high risk generation. Compared with the prior art, the privacy protection capability and the antagonistic robustness can be remarkably improved while the image generation quality is maintained, and the balance between the performance and the safety is realized.

The invention relates to preparation of a xanthine (Xanthine) detection kit by using a chemiluminescence immunoassay technology. Xanthine is purine widely existing in body fluid, tissues and plant cells and is small in molecular weight and poor in immunogenicity, and when a corresponding antibody is prepared, a hapten needs to be coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a xanthine hapten, preparation of a xanthine complete antigen and an anti-xanthine antibody, a method for measuring the concentration of xanthine, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise the design and chemical synthesis of xanthine derivatives. The xanthine derivative disclosed by the invention has a structure as shown in a formula (I): # imgabs0 #, wherein R is-(CH2) n-COOH, and n is any integer between 1 and 10.

The invention relates to a preparation method of a lactic acid (lactic acid) detection kit by using a chemiluminescence immunoassay technology. As lactic acid is small in molecular weight and poor in immunogenicity, when a corresponding antibody is prepared, firstly, a proper derivative site needs to be selected, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a lactic acid hapten, preparation of a lactic acid complete antigen and an anti-lactic acid antibody, a method for measuring the concentration of lactic acid, and composition and components of a reagent. The lactic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The present invention relates to a circular RNA for targeted inhibition of fish rhabdovirus replication, as well as its preparation method and application. The circular RNA is SCRV-circRNA-1, whose nucleotide sequence is shown in SEQ ID NO:1. The circular RNA is prepared by cyclizing linear SCRV-circRNA-1 with T4 RNA ligase 1, followed by removal of residual linear RNA in the reaction system using RNase R. SCRV-circRNA-1 has strong binding ability with SCRV-N protein, can effectively inhibit the replication of SCRV virus, and can be used in the field of aquaculture to prepare vaccines, antiviral drugs, and other biological immune products for fish rhabdovirus.

The invention belongs to the technical field of biological immunity, and particularly relates to a monoclonal antibody for cat Fel d7 and application of the monoclonal antibody. By screening the high-affinity monoclonal antibody aiming at the Fel d7, the detection of the Fel d7 as low as nanogram level (ng / mL) is realized, meanwhile, the cross reaction with other proteins in a cat body is avoided by utilizing the high uniformity of the monoclonal antibody and only recognizing a certain specific epitope, so that the detection specificity is improved, and compared with a polyclonal antibody, the Fel d7 monoclonal antibody is more stable and stronger in specificity; according to the present invention, the monoclonal antibody is adopted to detect the cat Fel d7, the interference of the non-specific reaction is avoided, the double-antibody sandwich ELISA kit formed based on the monoclonal antibody and used for detecting the cat Fel d7 is adopted as the coating antibody and the detection antibody so as to achieve the synergistic effect to amplify the detection signal, and the biotin-streptavidin-HRP three-stage signal amplification system is adopted so as to achieve the high-precision detection of the Fel d7;

The invention belongs to the technical field of biological immunodetection and nano sensing, and particularly discloses a colorimetric / SERS (Surface Enhanced Raman Scattering) dual-mode detection method for a digestive tract tumor marker based on gold coral at platinum nano enzyme, which comprises the following steps: preparing an Au NCs at Pt nano enzyme probe labeled with a labeled antibody (Ab2); the method comprises the following steps: mixing and incubating a sample to be detected, a nano-enzyme probe and a magnetic bead coated with a capture antibody (Ab1) in an optimized incubation solution, and forming an immune sandwich compound through a specific immune reaction; carrying out magnetic separation and washing on the immune sandwich compound, and finally concentrating and fixing the volume to a small volume; adding an enzyme substrate TMB (Tetramethylbenzidine) solution, and catalyzing TMB to be oxidized into oxTMB by utilizing peroxidase-like activity of the nano-enzyme; and S5, observing the color change of the oxTMB by adopting a colorimetric method and detecting a characteristic Raman signal by adopting SERS (Surface Enhanced Raman Scattering), so as to realize rapid, high-sensitivity and high-specificity dual-mode detection on the CA19-9. According to the method, the detection sensitivity and efficiency are remarkably improved by utilizing the excellent catalysis and SERS enhancement dual effects of the Au NCs (at) Pt nano-enzyme and combining a magnetic bead separation and enrichment technology.

The application relates to a preparation of a galactose detection kit by using a chemiluminescence immunoassay technique. Since the galactose has small molecular weight, poor immunogenicity, strong immunological tolerance, and has a D / L chiral structure, exists in a six-membered ring form in a solution, and can be converted into alpha and beta anomers. Therefore, when preparing a corresponding antibody, firstly, a suitable anomer and a derivative site need to be selected, and a derived hapten and a specific macromolecular carrier are coupled to prepare a complete antigen. The application successfully prepares an antibody with good specificity and strong affinity by designing and reforming the hapten. The application mainly relates to the design and synthesis of a galactose hapten, the preparation of a galactose complete antigen and an anti-galactose antibody, and a method for determining the concentration of galactose, the composition and ingredients of a reagent. The galactose derivative has the structure shown in formula (I).

The invention relates to a preparation method of a prourobilinogen (Urobilinogen) detection kit by using a chemiluminescence immunoassay technology. Joint detection of the prourobilirubin and the bilirubin is an important basis for clinically judging intrahepatic cholestasis, completely obstructive jaundice and hepatitis, and is also an important index of liver functions. Due to small molecular weight and poor immunogenicity of the urobilinogen, when a corresponding antibody is prepared, firstly, a proper derivative site needs to be selected, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. The invention mainly relates to design and synthesis of a urobilisin hapten, preparation of a urobilisin complete antigen and an anti-urobilisin antibody, a method for measuring the concentration of urobilisin, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise the design and chemical synthesis of a urobilinogen derivative. The urobilinogen derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention discloses an anti-human P14 polypeptide rabbit monoclonal antibody and application thereof, and relates to the technical field of biological immunodetection, the anti-human P14 polypeptide rabbit monoclonal antibody comprises a first group: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, DAS and SEQ ID NO.5; or, a second group: SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, DAS, and SEQ ID NO. 10; or, a third group: SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, DTS and SEQ ID NO.15. The invention also discloses a method for preparing the traditional Chinese medicine composition. The anti-human P14 polypeptide rabbit monoclonal antibody disclosed by the invention can specifically recognize the human P14 polypeptide and can be widely applied to detection of the human P14 polypeptide.

The application discloses an inhibitory antibody or antigen-binding fragment thereof against human OX40, a preparation method and application, and belongs to the technical field of biological immunity. The human-mouse chimeric antibody Y07-L2 and the humanized antibody Y07-C2 prepared by the application have similar EC50 in combination with human OX40, and are similar to the targeted antibody drug KHK-4083 in inhibiting OX40. The heavy chain variable region of the human-mouse chimeric antibody Y07-L2 is shown as SEQ ID NO. 14, and the light chain variable region is shown as SEQ ID NO. 15; the heavy chain variable region of the humanized antibody Y07-C2 is shown as SEQ ID NO. 20, and the light chain variable region of the humanized antibody Y07-C2 is shown as SEQ ID NO. 21; the CDR region of the humanized antibody Y07-C2 is subjected to mutation, and the mutant antibody Y07-C2MH4 and Y07-C2MH4L3 are obviously superior to other antibodies.

The invention relates to a preparation method of a gamma-aminobutyric acid (gamma-aminobutyric acid) detection kit by using a chemiluminescence immunoassay technology. Due to the fact that gamma-aminobutyric acid is small in molecular weight and poor in immunogenicity, the specificity and affinity of the prepared antibody cannot meet the requirements of an immunodetection reagent, and 4-acetaminobutyric acid with the large molecular weight is innovatively used for hapten design. In the detection process, gamma-aminobutyric acid in a sample is converted into 4-acetaminobutyric acid only by acetylation treatment through a specific sample treatment solution, and the content of gamma-aminobutyric acid in the body is indirectly reflected by measuring the concentration of 4-acetaminobutyric acid in the sample. The invention mainly relates to design and synthesis of a gamma-aminobutyric acid hapten, preparation of a gamma-aminobutyric acid complete antigen and an anti-gamma-aminobutyric acid antibody, a method for measuring the concentration of gamma-aminobutyric acid, and composition and components of a reagent. The gamma-aminobutyric acid derivative disclosed by the invention has a structure as shown in a formula (I).

This invention relates to a method for preparing a levodopa detection kit using chemiluminescent immunoassay. Due to the small molecular weight and poor immunogenicity of levodopa, and its presence of catechol in its structure, it is easily oxidized in neutral or alkaline environments. Therefore, when preparing the corresponding antibody, it is necessary to first select a suitable derivatization site and conjugate the derived hapten to a specific macromolecular carrier to prepare the complete antigen. This invention successfully prepared antibodies with good specificity and strong affinity through the design and modification of the hapten. This invention mainly relates to the design and synthesis of levodopa haptens, the preparation of complete levodopa antigens and anti-levodopa antibodies, and the methods, reagent composition, and components for determining levodopa concentration. The hapten design and synthesis of this invention mainly includes the design and chemical synthesis of levodopa derivatives. The levodopa derivative of this invention has the structure shown in formula (I): Formula (I).

The invention belongs to the technical field of biological immunity, and particularly relates to a monoclonal antibody for cat Fel d1 and application of the monoclonal antibody. By screening the high-affinity monoclonal antibody aiming at the Fel d1, the detection of the Fel d1 as low as a pictogram level (pg / mL) is realized, meanwhile, the cross reaction with other proteins in a cat body is avoided by utilizing the high uniformity of the monoclonal antibody and only recognizing a certain specific epitope, so that the detection specificity is improved, and compared with a polyclonal antibody, the Fel d1 monoclonal antibody is more stable and stronger in specificity; according to the present invention, the monoclonal antibody is adopted to detect the cat Fel d1, the interference of the non-specific reaction is avoided, the double-antibody sandwich ELISA kit formed based on the monoclonal antibody and used for detecting the cat Fel d1 is adopted as the coating antibody and the detection antibody so as to achieve the synergistic effect to amplify the detection signal, and the biotin-streptavidin-HRP three-stage signal amplification system is adopted so as to achieve the high-precision detection of the Fel d1.