59 results about "Rhabdovirus carpio" patented technology

This invention relates to a composition comprising a recombinant or genetically engineered Rhabdovirus that expresses a Fusion Protein, such as the F protein of the Paramyxovirus SV5 strain. This recombinant Rhabdovirus may express other non-Rhabdovirus attachment proteins and/or an enhancer protein. The invention also relates to methods of making recombinant Rhabdoviruses which express an F Protein. These recombinant compositions can be used for purposes of research, as well as for diagnostic and therapeutic compositions for treatment of diseases.

The invention belongs to the technical field of virus detection, and specifically relates to a triple fluorescent PCR detection kit for the infectious spleen and kidney necrosis virus, largemouth bassranavirus and siniperca chuatsi rhabdovirus. The kit of the invention comprises virus-specific amplification primers and probes, a positive standard, a negative standard, Taq enzyme, reverse transcriptase, a RNA enzyme inhibitor, a reaction buffer, dNTP, nuclease-free water and a freeze-drying protective agent. The kit of the invention can perform multiple channel detection at the same time by using triple fluorescent PCR, uses the different fluorescently labeled probes, can detect the three viruses simultaneously in one reaction system, reduces the detection difficulty, shortens the detection time, and can help raisers accurately get detection results in time. The kit of the invention has high detection sensitivity, high stability and strong specificity, has no cross-reactions with otheraquatic viruses, and can be used for early monitoring and prevention and control of epidemic diseases.

The invention relates to a siniperca chuatsi ranairidovirus and rhabdovirus duplex PCR detection kit and detection method, and belongs to the technical field of PCR. The siniperca chuatsi ranairidovirus and rhabdovirus duplex PCR detection kit provided by the invention comprises primers, EasyTaq PCR SuperMix, a negative control solution and a positive control solution, wherein the primers comprisea primer designed by aiming at an MCP gene conserved region of the siniperca chuatsi ranairidovirus, and a primer designed by aiming at an N gene conserved region of the siniperca chuatsi rhabdovirus. The siniperca chuatsi ranairidovirus and rhabdovirus duplex PCR detection kit and detection method provided by the invention can be used for fast, efficiently and accurately detecting the sinipercachuatsi ranairidovirus and the siniperca chuatsi rhabdovirus at the same time; the time and the labor are saved; more treatment time is won for sick siniperca chuatsi; and important significance is realized on subsequent study, prevention and control.

Part of protein fragment G2 of MSRV glycoprotein is selected, G2 protein is obtained through prokaryotic recombinant expression, and mannosylation modification is carried out on the G2 protein to prepare a perch rhabdovirus nano-carrier targeting vaccine, and research shows that for perch after immunized by the subunit vaccine for 21 days is attacked by an MSRV FJ985 strain, the perch immune protection rate is 94%-96%, and the vaccine is safe and effective. After the perch is immunized, a better protection effect can be achieved, and the perch can effectively resist the attack of MSRV virulent virus.

The invention belongs to the virus detection field, in particular, discloses a Chinese rice virus-Field eel rhabdovirus Cr-ERV and RT-PCR detection primer and an application thereof. The Monopterus albus rhabdovirus is isolated from diseased Monopterus albus seedlings, and the virus is (Chinese rice virus-Field eel rhabdovirus) CrERV, deposited as CCTCC NO: V201819, is of pioneering significance for the study of Monopterus albus rhabdovirus. Primers are designed to detect rhabdovirus of Monopterus albus. The primers have good specificity and high sensitivity.

The Siniperca chuatsi rhabdovirus, SCRV0208, with preservation number of CCTCC No. V202008, is prepared through culture of GCF0208 cell in TC199 culture medium with ox serum to grow single layer of cell, inoculation with virus liquid, sampling in different stages, slicing, electronic microscope observation to determine the dot picking time, picking dot and inoculating to the single layer of cell for enlarged culture, and centrifugating to obtain pure virus. The said process is simple and practical, can separate rhabdovirus from accompanied virus to obtain high purity virus antigen for application in cell engineering vaccine and genetic engineering vaccine.

A Scophthalmus maximus rahbdovirus (SMRV0207, CCTCC No.202006) is prepared through sampling the liver, kidney, heart and spleen tissues from the scophthalmus maximus in ill, shearing, adding PBS, homogenizing, adding double antibody, freezing, thawing, centrifugal extracting, filtering, inoculating it to CLC 0207 cell, and constant-temp. continuous culture. Its advantages are high reproduction speed, and high reproduction valency up to 10 to the power 8 TCID 50/ml. It can be used to prepare vaccine.

The invention discloses a preparation method of mandarin fish rhabdovirus glycoprotein expressed by recombinant baculovirus. The preparation method comprises the following steps: designing and synthesizing primers; amplifying and purifying a target gene; constructing a recombinant donor plasmid pFastBac-G; constructing a recombinant shuttle plasmid rBacmid-G; preparing a recombinant baculovirus; purifying the target protein; and carrying out Western blot detection. According to the invention, a large amount of soluble recombinant proteins with good antigenicity and immunogenicity and similar functions to natural proteins can be obtained, and the method is suitable for high-efficiency expression of mandarin fish rhabdovirus glycoproteins and preparation of DNA vaccines.

The present invention includes bicyclic compounds that are useful in preventing or treating viral infections, such as viral infections caused by a filovirus, arenavirus, rhabdovirus, paramyxovirus, and/or retrovirus. The present invention further includes compositions comprising such compounds, and methods of treating a viral infection in a subject using such compounds.

The invention discloses application of a PARP inhibitor Rucaparib in preparation of fish rhabdovirus resisting products. According to the application, a plurality of PARP inhibitors are screened, andthe result shows that the PARP inhibitor Rucaparib can inhibit mRNA replication of viruses IHNV, VHSV and SVCV in cells and reduce the titer (TCID50) of the viruses IHNV, VHSV and SVCV; the inhibitioneffect of Rucaparib on IHNV, VHSV and SVCV is in dose-dependent relation. The PARP inhibitor Rucaparib is expected to become a novel drug for resisting fish rhabdovirus.

Disclosed herein is an oncolytic recombinant Maraba virus whose genome comprises one or more nucleic acid sequences that, in combination, encode an interleukin-12 (IL12) protein or a functional portion thereof. A method for treating a cancer in a patient using the oncolytic recombinant Maraba virus is also disclosed. The present disclosure also provides a tumour cell infected with an oncolytic rhabdovirus whose genome comprises one or more nucleic acid sequences that, in combination, encode an interleukin-12 (IL12) protein or a functional portion thereof, for use as an infected cell vaccine (ICV) for the treatment of a cancer. A method for treating a cancer in a patient using the infected cell vaccine is also disclosed.

The invention discloses a method and a kit for detecting reverse transcription-loop mediated isothermal amplification (RT-LAMP) of pike fry rhabdovirus (RFRV), wherein an adopted outer primer contains sequences indicated by Seq ID No.1 and Seq ID No.2 respectively. According to the method and the kit which are provided by the invention, PFRV can be detected specifically; and the sensitivity is high and particularly reaches 100.5TCID50 when the kit is used for detecting the extracted nucleic acid in a virus suspension.

The invention discloses an application of a PARP inhibitor PJ34 in preparation of an anti-fish rhabdovirus product. The invention provides an application of a PARP inhibitor PJ34 or a derivative thereof or a pharmaceutically acceptable salt thereof or a substance taking the PARP inhibitor PJ34 or the derivative thereof or the pharmaceutically acceptable salt thereof as an active ingredient in preparation of an anti-fish rhabdovirus product. In the invention, a plurality of PARP inhibitors are screened, and results show that the PARP inhibitor PJ34 can inhibit mRNA replication of IHNV, VHSV andSVCV viruses in cells and reduce titer (TCID50) of the IHNV, the VHSV and the SVCV viruses at the same time; and the inhibition effect of the PJ34 on the IHNV, the VHSV and the SVCV shows a dose-dependent relationship. The PARP inhibitor PJ34 is expected to become a novel drug for resisting fish rhabdovirus.

The invention belongs to the field of aquatic organism cells and the technical field of aquaculture disease prevention and control, and discloses a rhabdovirus-sensitive monopterus albus kidney tissuecell line and application. The cell line in the invention is preserved in the China centre for type culture collection; and the preservation number is CCTCC NO:C2019286. The monopterus albus kidney tissue cell line (CrE-K) has a good growth state and is sensitive to CrERV newly discovered, separated and identified at present; after continuous passage of CrERV on CrE-K cells to the 16th generation, viral nucleic acid can still be detected; cytopathy is stable; the cells with the pathological change effect are subjected to ultrathin electron microscopy slicing; and a large number of CrERV mature virus particles and the replication process thereof can be observed in the CrE-K cells. The construction method of the monopterus albus kidney tissue cell line in the invention is high in repeatability, scientific and reasonable in condition and suitable for in-vitro culture of the rhabdovirus; and a technical platform is provided for separation, detection and culture of the rhabdovirus and complete biological characteristic research of the rhabdovirus.

The invention discloses an LAMP detection primer group for Siniperca chuatsi-perch rhabdovirus, application of the LAMP detection primer group and a detection kit. The primer group comprises a pair ofouter primers, a pair of inner primers and a pair of loop primers; the outer primers comprise a sequence L-F3 and a sequence L-B3, the sequence L-F3 of the outer primers is shown in SEQ ID NO: 1, andthe sequence L-B3 of the outer primers is shown in SEQ ID NO: 2; the inner primers comprise a sequence L-FIP and a sequence L-BIP, the sequence L-FIP of the inner primers is shown in SEQ ID NO: 3, and the sequence L-BIP of the inner primers is shown in SEQ ID NO: 4; and the loop primers comprise a sequence L-LF and a sequence L-LB, the sequence L-LF of the loop primers is shown in SEQ ID NO: 5, and the sequence L-LB of the loop primers is shown in SEQ ID NO: 6. The invention further provides application of the primer group in preparation of a Siniperca chuatsi-perch rhabdovirus detection kitand the kit. The primer group and kit, provided by the invention, have the advantages of simplicity in operation, high detection speed, good specificity, high sensitivity, reliable result, and the like.

The invention discloses a triple PCR primer group for rapidly detecting three fish viruses and application thereof, and relates to the technical field of gene detection. The invention discloses a triple PCR primer group for rapidly detecting three fish viruses. The triple PCR primer group comprises an infectious spleen and kidney necrosis virus ISKNV primer, a mandarin fish frog virus SCRIV primerand a mandarin fish rhabdovirus SCRV primer, the triple PCR primer group has high sensitivity, the detection time is 2-3 hours earlier than that of conventional PCR, the efficiency of clinical diagnosis is greatly improved, richer reference bases can be provided for epidemiological investigation of ISKNV, SCRIV and SCRV infection, and the triple PCR primer group has important significance for subsequent research and prevention and treatment.

The invention relates to a pharmaceutical composition for treating tumors or cancers and application thereof. Particularly, the pharmaceutical composition comprises an oncolytic rhabdovirus deliveredthrough direct local injection or systemic administration or intratumoral delivery, and the oncolytic rhabdovirus can generate a synergistic inhibition effect on tumors and/or cancers in combination with administration of a CD38 small-molecule inhibitor. Besides, the oncolytic rhabdovirus has the characteristic of recognizing tumor cells and cannot damage normal cells, meanwhile, the CD38 small-molecule inhibitor has the specific T cell receptor molecule inhibiting activity, and the safety and the curative effect are remarkably excellent through combined use of the oncolytic rhabdovirus and the CD38 small-molecule inhibitor.

The invention relates to cell screening, in order to solve rhabdovirus pollution in an sf9 cell strain, the invention provides a serum-independent rhabdovirus-pollution-free sf9 cell strain, the cell strain is named as an sf9-RF cell strain, and the biological preservation number of the cell strain is CGMCC No.45028. The invention also provides a method for preparing the sf9 cell strain. The invention further provides a screening method of the serum-free rhabdovirus-pollution-free sf9 cell strain, and screening is carried out under a serum-free condition. The invention also provides an application of the serum-independent rhabdovirus-pollution-free sf9 cell strain in foreign protein expression or AAV production based on a baculovirus expression system. According to the serum-free rhabdovirus-pollution-free sf9 cell strain disclosed by the invention, a serum-free insect cell culture medium is used in the whole cell culture and screening process, and any exogenous virus is not introduced, so that various indexes of the cell line meet the requirements of cell matrixes for production, and when the cell line is applied to an expression system of baculovirus, the cell line can be used for preparing the rhabdovirus-pollution-free sf9 cell strain. And the safety of biological products can be effectively ensured.

The invention discloses a fluorescent quantitation PCR detection primer and kit for a Sf-rhabdovirus. The nucleotide sequence of the fluorescent quantitation PCR primer is disclosed by SEQ ID NO.1-SEQID NO.2. The fluorescent quantitation PCR primer disclosed by the invention can specifically detect the Sf-rhabdovirus, is high in detection sensitivity, has an extremely good linear relationship ina copy range of 8E*10-10, efficiently detects the Sf-rhabdovirus and improves detection efficiency.

The invention discloses a method for preparing and purifying oncolytic virus, and a recombinant oncolytic rhabdovirus. According to the preparation method, Vero cells are utilized to prepare oncolyticviruses (especially VSV viruses). Firstly, virus amplification conditions are optimized by utilizing the Vero cells, wherein the optimized conditions include virus infection complex number (MOI), virus diluent, virus amplification liquid, serum concentration during amplification, cell culture volume, virus harvesting time and the like; furthermore, virus purification conditions are optimized, wherein the optimized conditions include centrifugal force, sucrose bottoming centrifugation, centrifugation time, step-by-step centrifugation and the like; the VSV virus particles infect the vero cellsfor 48 h in a 3% FBS culture medium according to a proportion that MOI is equal to 5, and virus liquid is harvested; and after 26000*g centrifugation is conducted for 1 h at a temperature of 4 DEG C,28000*g centrifugation is conducted (30 min to 45 min), and it is determined that the high-titer VSV virus can be stably prepared under the step-by-step centrifugation condition. The preparation method is efficient, stable and cost-saving, provides technical support for subsequent large-scale production of the VSV virus, and can be used for preparing anti-tumor virus drugs.