5 results about "Epidemiologic study" patented technology

The application discloses a triple fluorescent quantitative PCR detection kit for simultaneously detecting CHIKV, BFV and RRV, which contains a specific primer probe composition for simultaneously detecting chikungunya virus, Barmah Forest virus and Ross River virus, can simultaneously detect three viruses which are transmitted by insect vectors and cause acute fever with arthralgia symptoms in human beings in one PCR reaction tube, and has excellent specificity and repeatability, and is suitable for routine laboratory diagnosis of suspected samples of alphavirus infection, and provides a rapid and accurate detection method for the epidemiological study of CHIKV, BFV and RRV.

The application discloses a kind of detection group of triple RT-qPCR primers of group A porcine rotavirus G4, G5 and G9 strain, kit and its application, belong to molecular biology field.Primer group includes primer and probe, nucleotide sequence is as shown in SEQ ID NO.1-SEQ ID NO.8.The application can realize the simultaneous detection of group A porcine rotavirus G4, G5 and G9 genotype strain in one PCR reaction tube, and has good specificity, sensitivity and repeatability, and can be directly applied to the laboratory diagnosis of pig clinical diarrhea sample pathogen, provides a kind of fast, accurate detection method for the epidemiological study and clinical differential diagnosis of group A porcine rotavirus G4, G5 and G9 genotype, especially suitable for scientific research and clinical application, with good commercial application value.

This application relates to the field of in vitro diagnostics and microbial detection technology, and in particular to a method and kit for optimizing reaction reagents for vaginal flora detection. The method includes serial dilution of 2×One-Step SYBR Green reagent and iScript reverse transcriptase in an RT-qPCR system, and supplementing the diluent with 0.2 mmol / L dNTPs and 1 mmol / L Mg. 2+ Achieving the reaction of 10 in an 8 μL reaction system 3 -10 8 Stable amplification of cellular equivalent RNA. This application reduces reagent usage to one-third of the original protocol while maintaining amplification efficiency, sensitivity, and reproducibility, significantly lowering detection costs and making it suitable for high-throughput screening and epidemiological studies.

The present application relates to the technical field of nucleic acid detection, and discloses a general primer pair for detecting the genus of Segniliparus and application thereof. The detection primer pair and identification method provided by the present application are applicable to all species of the genus of Segniliparus, including but not limited to Segniliparus rugosus, and especially unnamed ANI species. The amplification method provided by the present application has low requirements for templates, and is applicable to clinical original samples such as sputum, alveolar lavage fluid, pleural and peritoneal fluid, culture, and environmental original samples such as water, soil and air, and extracted nucleic acid, and does not depend on culture. The method is fast and low-cost, and has low requirements for equipment and detection personnel, and is applicable to laboratories at all levels. The detection reagent based on the present application can be widely applied to related fields such as environmental monitoring, clinical precise detection and epidemiological research.

The present invention relates to a highly sensitive nested multiplex PCR-based primer panel for the detection of Lumpy Skin Disease Virus (LSDV) in a biological sample obtained from bovine species and livestock animals In particular, the present invention provides a nested multiplex detection primer panel and a kit comprising the same, for the detection of Lumpy Skin Disease Virus (LSDV) and its variants thereof. The present invention also provides a multiplex primer panel for the PCR whole genome amplification and genotyping of Lumpy Skin Disease Virus (LSDV) to obtain genomic data, which can be useful in tracking the evolution of the Lumpy Skin Disease virus and correlating with disease severity. Therefore, the present invention emphasizes its utility in veterinary diagnostics, epidemiological studies, and disease management strategies related to vaccine development for Lumpy Skin Disease Virus (LSDV) infections in livestock.