39 results about "Epidemiologic study" patented technology

The invention encompasses methods, including computer-implemented methods, of defining a virtual patient population and mapping the virtual patient population to a population of real patients. The invention utilizes virtual measures from one or more virtual patients, and data representative of multiple real subjects in a sample population, such as data collected from patients in a clinical trial or epidemiological study of a real population. The invention includes evaluating the similarity between the virtual patients and the real subjects, and assigning prevalences to the virtual patients based on the evaluation. The similarity can be assessed using some or all of the virtual measures of the virtual patients and some or all of the data obtained for the real subjects. Any of various goodness-of-fit measures can be used to evaluate the similarity or to help identify prevalences. The virtual patient population is defined as the virtual patients according to their respective prevalences.

Methods and apparatus for improving measurements of cardiovascular health status in a given individual are provided. The comprehensive assessment of cardiovascular health includes at least two components: risk factor assessment based on epidemiologic studies and functional status of the individual. Structural studies of the individual can also be included in the comprehensive assessment of cardiovascular health. The invention aims to improve detection, treatment, devices, and administration of cardiovascular risk assessment.

Methods and apparatus for improving measurements of cardiovascular health status in a given individual are provided. The comprehensive assessment of cardiovascular health includes at least two components: risk factor assessment based on epidemiologic studies and functional status of the individual. Structural studies of the individual can also be included in the comprehensive assessment of cardiovascular health. The invention aims to improve detection, treatment, devices, and administration of cardiovascular risk assessment.

The invention discloses a hybridoma cell strain 6D6 of a specificity monoclonal antibody capable of continuously and stably secreting foot-and-mouth disease resistant O-type (O / GX / 09-7) viruses, and the specificity monoclonal antibody secreted by the hybridoma cell strain. The 6D6anti can recognize the foot-and-mouth disease O-type (O / GX / 09-7) viruses in a specificity mode, the 6D6anti is used as a coating and an enzyme labeled antibody in ELISA detection and can be used for detecting the foot-and-mouth disease O-type (O / GX / 09-7) viruses, and the advantages of high specificity and high sensitivity are achieved; the hybridoma cell strain, the antibody and the application can play an important role in detection of the foot-and-mouth disease O-type (O / GX / 09-7) viruses, vaccine production and epidemiologic study.

The invention discloses a primer combination used for detecting SARS-CoV-2 whole genome. The primer combination comprises specific primers used for SARS-CoV-2RNA reverse transcription and a primer group for amplification of the whole genome; by adoption of the primer combination, after target genes are amplified by reverse transcription and nest type PCR, on the basis of a next-generation semiconductor sequencing platform, a next-generation semiconductor sequencing technology used for detecting the SARS-CoV-2 whole genome and mutation sites is constructed; the method has the advantages of being wide in applicability, high in flux and good in accuracy, and can detect multiple samples one time; the convenient method is provided for deep analysis of the SARS-CoV-2 genome; and the method has important meanings for epidemiologic study, clinical early molecular diagnosis and the like.

The invention discloses a malaria serum with an anti-tumor function and a preparation method and application of the malaria serum with the anti-tumor function.According to long-term arduous scientific experiments, the serum of patients with malaria has a certain anti-tumor function, and particularly the serum of patients with a syndrome of malaria and thrombocytopenia is effective in treatment or prevention of tumors, especially liver cancers, under specific concentration conditions.According to experiments, the serum has an evident apoptosis induction function on hepatoma cell strains (7721) cultured in vitro, and the hepatoma cell apoptosis induction ratio is increased along with increase of serum concentration.The malaria serum with the anti-tumor function and the preparation method and application of the malaria serum with the anti-tumor function provide theoretical and experimental bases for further researches on tumor cell apoptosis induction factors generated by plasmodia, breaks a new path for rediscovery pathogenesis of critical and cerebral malaria cases and epidemiologic researches of various plasmodium species and strains, and make new effort in application of the malaria serum or preparations thereof to preparation of medicines for treatment or prevention of the tumors, thereby having great scientific research values and significances.

The invention discloses a method for detecting canine distemper virus by using a one-step reverse transcription-polymerase chain reaction (RT-PCR) method. The method comprises the following steps of: preprocessing a sample for the RT-PCR detection; extracting total ribonucleic acid (RNA); obtaining a specific amplification product by one-step RT-PCR reaction; and performing 1 percent agarose gel electrophoresis identification on the amplification product. The method of the invention has the characteristics of high flexibility, high specificity, simple and convenient operation procedure, low contamination rate and reliable detection result; the acquired sequence information is directly used for analyzing genetic derivation relation of an epidemic strain; and the method is suitable for etiological diagnosis and epidemiological research of canine distemper.

The invention discloses a PCR kit for detecting ST251-type aeromonas hydrophila and application. The kit comprises a PCR Premix, a primer pair, a standard positive DNA template, a standard negative DNA template and sterilization double distilled water. An upstream primer sequence is 5'-GCGGCGTAAGCGGATTTGTAGC-3', a downstream primer sequence is 5'-CCGGTCGGATTGGTCAGGTTCAT-3', the annealing temperature is 63 DEG C, a correct amplification product is 826bp in size, the standard positive DNA template is genomic DNA extract of an ST251-type aeromonas hydrophila XS91-4-1 strain, and the DNA concentration is 500 nanograms per microliter; the negative DNA template is genomic extractive of an aeromonas veronii IB340 strain, and the DNA concentration is 500 nanograms per microliter. The kit is applicable to all kinds of gene amplification instruments, high in detection specificity and sensitivity, quick, accurate and good in stability. The kit can be used for quickly diagnosing freshwater fish outbreak diseases and blood poisoning caused by aeromonas hydrophila of other fishes and quickly detecting and identifying bacteria and then is widely applied to disease prediction and forecast and epidemiologic studies.

The invention is to provide a method and kit based on recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) for detection of caprine arthritis-encephalitis virus (CAEV) infection. The method and kit are suitable for both laboratory and field application, and are specific and sensitive for detecting CAEV proviral DNA in goats in a fast manner. The method and lit of the invention are also applicable for on-site utilization at farms and should be useful in both eradication programs and epidemiological studies.

The invention discloses a hybrid snakehead rhabdovirus fluorescent quantitative PCR (polymerase chain reaction) detection kit and a detection method thereof. The kit contains specific primers HSHRV-2F and HSHRV-2R and a probe HSHRV-2probe. When the kit is used for detecting hybrid snakehead rhabdovirus, the kit has the characteristics of high sensitivity, high repetitiveness and good specificity, can quickly and accurately realize qualitative and quantitative detection of hybrid snakehead rhabdovirus, and is of far reaching importance in early diagnosis of hybrid snakehead virus diseases, molecular epidemiology research, prevention and control technology research and the like.

The invention discloses a method for simultaneously detecting three groups of rotaviruses A, B and C, and belongs to the field of biological detection. A pair of specific primers and a specific probewith different fluorescent labels are designed for target genes of group A rotavirus, group B rotavirus and group C rotavirus, a pair of universal primers is further designed, and during PCR amplification, the specific primers with universal primer tags are used for enrichment and amplification; and then universal primer tags are used for exponential amplification, and fluorescence signals of exponential amplification products are collected for detection. The method can simultaneously detect the group A rotavirus, the group B rotavirus, and the group C rotavirus. The method is high in specificity, high in sensitivity and short in detection time, and can be used for early rapid diagnosis of rotavirus infection and epidemiological study of the rotavirus.

A monoclonal antibody for H1N1 swine influenza A virus hemagglutinin protein and a double-antibody sandwich ELISA kit are disclosed. The kit comprises a solid phase vector coated with the monoclonal antibody, a rabbit polyclonal antibody labeled by horseradish peroxidase, an H1N1 hemagglutinin protein standard substance, a sample diluting liquid, a washing liquid, a substrate coloring liquid and a reaction terminating liquid. The kit is good in sensitivity, capable of performing quantitative detection for the swine influenza virus hemagglutinin protein, and specifically recognizing main prevalent strains of A(H1N1) influenza outbreak in 2009, and free of cross reactions with other strains of the H1N1 influenza viruses and other influenza virus subtypes. The kit is simple to operate, can be used for supporting fundamental research of the H1N1 swine influenza virus, and has important significance for performing epidemiologic study on the H1N1 influenza viruses in different zones and different years.

The invention discloses a duck-sourced H4N6 subtype avian influenza virus strain and an application thereof. The preservation No. of the virus strain is CGMCC NO.7606. The virus strain is duck-sourced H4N6 subtype avian influenza virus containing 8 fragments of which the CDS sequence shown in SEQ ID NO.1-SEQ ID NO.8, wherein the 8 fragments respectively correspond to PB2, PB1, PA, HA, NP, NA, M and NS. Whole genome sequencing is carried out on the virus strain, and the information is used for research on the detection, distribution and epidemic rule of the avian influenza virus as well as control of prevalence and spreading of the avian influenza. The virus strain supplements valuable genetic information of the H4N6 subtype avian influenza virus in China, provides valuable information for molecular evolution and epidemiologic study of H4N6 subtype avian influenza virus and can be used for preparing reagents for rapidly diagnosing H4N6 subtype influenza virus infection and vaccine study.

The invention provides a related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance. According to the related gene, method, primer group and kit, not only can the mycobacterium tuberculosis complex flora be identified, but also 16 common anti-tuberculosis first-line and second-line drug resistance gene mutations of the mycobacterium tuberculosis complex flora can be distinguished and detected at high resolution, and rapid, simultaneous and accurate drug resistance detection is achieved. The related gene, method, primer group and kit can be used for clinical treatment reference and epidemiological research.

Device, method, and system for nanoparticle capture, tracking, and / or detection. A functional paper-based platform is modified with capture ligands to create binding sites for nanoparticles. According to an embodiment, nanoparticle binding produces visual images of the particle content and distribution on the modified sensing surface, which provides capabilities for both NP sequestration and real-time detection. According to an embodiment the system may be utilized for environmental decontamination, fabrication of personal protective equipment, field monitoring, and epidemiological studies. The availability of inexpensive and easy-to-use quantitative methods can facilitate rapid assessment and measurement of NPs concentration and the level of exposure for large scale toxicological and epidemiological testing

The invention discloses a kit for detecting a foot-and-mouth disease type-A virus, a preparation method of the kit and a using method of the kit used for achieving aims except for disease diagnosis. Primers for amplifying the foot-and-mouth disease type-A virus have four gene sequences; and the kit for detecting the foot-and-mouth disease type-A virus internally comprises the four primers with the gene sequences. Relevant experiments prove that nucleic acid capable of specifically and rapidly amplifying the foot-and-mouth disease type-A virus is capable of detecting a specific band through nucleic acid electrophoresis and also has specificity. The relevant experiments hint that the sequences can be applied to preparation of GeXP diagnosis kits for rapidly identifying the foot-and-mouth disease type-A virus and epidemiologic study of the foot-and-mouth disease virus.

The invention provides a human enterovirus71-type C4-subtype lethal strain SD095 with the accession number of CGMCC (China General Microbiological Culture Collection Center) NO.6601 and its application. A rat adaptive lethal strain is acquired by conventional virus isolation cultivation, animal infection and a series of technical means, and an animal lethal model based on the virus strain SD095 can be established. The acquisition of the lethal virus strain and the establishment of the animal lethal model have important significance for promoting clinical treatment and researches in China on diseases and death cases caused by EV71 (human enterovirus 71) virus, and have important values for further promotion of experimental and clinical studies and epidemiologic studies on the EV71, and new vaccine development and evaluation.

The invention relates to an algal bloom prediction method based on remote sensing total algae quantity, which comprises the following steps: performing big data analysis on algal bloom occurrence condition, remote sensing total algae quantity and different meteorological indexes monitored by remote sensing in historical periods, and then constructing a Logistic prediction model of algal bloom occurrence probabilities in different lake areas, thereby realizing algal bloom occurrence probability prediction based on the remote sensing total algae quantity. According to the method, Logistic regression analysis which is mostly used in epidemiological research is adopted, and the (continuous) prediction of the algal bloom occurrence probability is realized.

The invention discloses an epidemiological research population screening method based on medical big data and a storage medium. The method comprises the following steps: acquiring health medical data; determining to-be-constructed target population types according to requirements of epidemiological research, wherein the to-be-constructed target population types comprise a cross section research population, a fixed queue research population and a dynamic queue research population; setting screening conditions including a research time range, follow-up visit time, a time window, an inclusion rule and an ending rule; and screening records meeting the requirements of the target crowd from the health medical data, and constructing the target population. According to the medical big data-based epidemiological research population screening method and the storage medium, the target population is directly screened from the existing massive medical data to be brought into epidemiological research, and the target population comprises a cross section research population, a fixed queue research population and a dynamic array research population; the efficiency and correctness of epidemiological research population screening based on medical big data are improved, and manpower is saved.

The invention discloses a PCR kit for detecting ST251-type aeromonas hydrophila and application. The kit comprises a PCR Premix, a primer pair, a standard positive DNA template, a standard negative DNA template and sterilization double distilled water. An upstream primer sequence is 5'-GCGGCGTAAGCGGATTTGTAGC-3', a downstream primer sequence is 5'-CCGGTCGGATTGGTCAGGTTCAT-3', the annealing temperature is 63 DEG C, a correct amplification product is 826bp in size, the standard positive DNA template is genomic DNA extract of an ST251-type aeromonas hydrophila XS91-4-1 strain, and the DNA concentration is 500 nanograms per microliter; the negative DNA template is genomic extractive of an aeromonas veronii IB340 strain, and the DNA concentration is 500 nanograms per microliter. The kit is applicable to all kinds of gene amplification instruments, high in detection specificity and sensitivity, quick, accurate and good in stability. The kit can be used for quickly diagnosing freshwater fish outbreak diseases and blood poisoning caused by aeromonas hydrophila of other fishes and quickly detecting and identifying bacteria and then is widely applied to disease prediction and forecast and epidemiologic studies.

The invention relates to the technical field of biology, and discloses a chemiluminiscence kit for detecting anti-envelope glycoprotein (anti-E1 and anti-E2) antibodies in serum of an HCV infected person. The kit comprises a microwell plate obtained by coating with a recombinant HCV envelope glycoprotein (E1, E2) antigen, and a sample diluent, a negative control, a positive control, an enzyme marker working solution, a substrate solution A, a substrate solution B and a washing solution which are independently packaged, wherein the substrate solution A comprises luminol, o-phenylphenol, 4-imidazole phenol and a carbonic acid buffer solution (CB), and the substrate solution B comprises urea peroxide and a phosphate buffer solution (PB). According to the invention, the kit can be used for carrying out in-vitro qualitative / quantitative determination on anti-E1 and anti-E2 antibodies in serum of an HCV infected person, establishing a detection technology of the anti-E1 and anti-E2 antibodies in the serum of the HCV infected person and carrying out methodological evaluation, and can be applied to screening diagnosis, disease monitoring, prognosis evaluation, disease mechanism and epidemiological research of the HCV infected person.

The invention discloses a quadruple fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit for detecting porcine epidemic diarrhea virus and porcine rotavirus and application of the quadruple fluorescent quantitative PCR detection kit. The kit comprises a hot start Taq DNA polymerase, enzyme-free water, a PCR reaction solution and a probe method fluorescent quantitative PCR matched Buffer, the four pairs of specific primers, the corresponding TaqMan probes and the reference substances are used for detecting the G1 genotype porcine epidemic diarrhea virus, the G2 genotype porcine epidemic diarrhea virus, the porcine group A rotavirus and the porcine group C rotavirus. The kit provided by the invention realizes simultaneous detection of four viruses capable of causing porcine diarrhea in one PCR reaction tube, has excellent specificity and repeatability, and can be directly applied to conventional laboratory diagnosis of porcine clinical diarrhea samples; and a rapid and accurate detection method is provided for epidemiological research of the porcine epidemic diarrhea virus and the porcine rotavirus.

The invention is to provide a method and kit based on recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) for detection of caprine arthritis-encephalitis virus (CAEV) infection. The method and kit are suitable for both laboratory and field application, and are specific and sensitive for detecting CAEV proviral DNA in goats in a fast manner. The method and lit of the invention are also applicable for on-site utilization at farms and should be useful in both eradication programs and epidemiological studies.

The invention belongs to the technical field of oxidative stress biomarker detection, in particular to a method for testing oxidative stress biomarkers in blood plasma with a low coefficient of variation. The method comprises the following steps: taking a mixture of blood plasma and an extractant with the mixed volume ratio of 1: 20 as a to-be-detected substance solution, taking a supernatant of the to-be-detected substance solution and carrying out fluorescence determination by adopting a fluorescence microplate reader under the conditions of three excitation or emission wavelengths. According to the method, the coefficient of variation of oxidative stress biomarkers in blood plasma is remarkably reduced, the inter-batch coefficient of variation of FlOPs is reduced to be less than 3.6%, and the intra-batch coefficient of variation is reduced to be less than 2.7%, so that a more reliable FlOPs measurement method is provided for future epidemiological research and potential clinical practice.

The invention discloses a cognition behavior intervention system for depression of a senile dementia family caregiver. The intervention comprises the components of a caregiver training module which isused for training professional nurses so that the professional nurses master an intervention program, an intervention method and a therapeutic communication skill; an intervention pointing object module which is used for screening the caregiver with a depression symptom; an intervention content module which is used for performing integrated cognition behavior intervention according to the characteristics of the Chinese local senile dementia family caregiver; an intervention time length module which is used for determining an intervention time length of five hours, a face-to-face interventiontime length of 1 hour in each month and a telephone follow-up intervention time length of 20-30 minutes; an intervention interval module which is used for face-to-face intervention in a frequency of one time in each month, telephone follow-up surveying intervention in a frequency of one time in each month, wherein the interval time between the face-to-face intervention and the telephone follow-upsurveying intervention is two weeks; and an intervention form module which is used for performing the face-to-face intervention and the telephone intervention. Ten centers for epidemiologic studies depression scale are used for testing and screening the caregiver who accords with a preset depression standard, thereby realizing a purpose of mitigating or alleviating depression of the caregiver.