11 results about "Epidemiologic study" patented technology

The application discloses a triple fluorescent quantitative PCR detection kit for simultaneously detecting CHIKV, BFV and RRV, which contains a specific primer probe composition for simultaneously detecting chikungunya virus, Barmah Forest virus and Ross River virus, can simultaneously detect three viruses which are transmitted by insect vectors and cause acute fever with arthralgia symptoms in human beings in one PCR reaction tube, and has excellent specificity and repeatability, and is suitable for routine laboratory diagnosis of suspected samples of alphavirus infection, and provides a rapid and accurate detection method for the epidemiological study of CHIKV, BFV and RRV.

The application discloses a kind of detection group of triple RT-qPCR primers of group A porcine rotavirus G4, G5 and G9 strain, kit and its application, belong to molecular biology field.Primer group includes primer and probe, nucleotide sequence is as shown in SEQ ID NO.1-SEQ ID NO.8.The application can realize the simultaneous detection of group A porcine rotavirus G4, G5 and G9 genotype strain in one PCR reaction tube, and has good specificity, sensitivity and repeatability, and can be directly applied to the laboratory diagnosis of pig clinical diarrhea sample pathogen, provides a kind of fast, accurate detection method for the epidemiological study and clinical differential diagnosis of group A porcine rotavirus G4, G5 and G9 genotype, especially suitable for scientific research and clinical application, with good commercial application value.

The invention discloses a triple fluorescent quantitative PCR detection kit for simultaneously detecting CHIKV, BFV and RRV, and the kit comprises a specific primer probe composition for simultaneously detecting chikungunya virus, Bama forest virus and Rosc river virus, three viruses which are transmitted through entomophilous and cause human acute fever with arthralgia symptoms can be simultaneously detected in one PCR reaction tube, the lowest detection concentration of CHIKV plasmid samples is 20 copies / microliter, the lowest detection concentration of RRV and BFV positive samples is 10 copies / microliter, and the kit has excellent specificity and repeatability, and can be used for detecting the acute fever with arthralgia symptoms of the human acute fever with the arthralgia symptoms of the human acute fever with the arthralgia symptoms of the human acute fever with the arthralgia symptoms. The kit is suitable for conventional laboratory diagnosis of suspected alphavirus infection samples, and a rapid and accurate detection method is provided for epidemiological research of CHIKV, BFV and RRV.

The invention discloses a HIV (human immunodeficiency virus), HBV (hepatitis B virus), HCV (hepatitis C virus) and TP (total protein) hypersensitive detection kit and detection method based on a digital PCR (polymerase chain reaction) platform. The kit comprises a primer and a probe, the design of the primer and the probe is optimized, and the molecular detection system has higher sensitivity by utilizing high affinity and strong mismatch recognition capability of the LNA probe, so that a single copy can be detected. The method possibly has huge potential in the aspects of large-scale screening and clinical diagnosis of blood donors and epidemiological research of pathogens.

This invention relates to the field of medical animal model construction technology, and in particular to a method for constructing an animal model of cardiomyopathy induced by the circulatory system and its application. This invention, through adventitia-35 injection, establishes for the first time an animal model of "Aβ25-35-induced cardiomyopathy via the adventitia-35 circulatory system" in SD rats. In this model, Aβ25-35 perfusion via the adventitia-35 myocardium of the lower limbs induces diffuse myocardial fibrosis and a decrease in ejection fraction in SD rats, consistent with epidemiological data. This model can be used to further study the pathogenic mechanism of Aβ25-35-induced cardiomyopathy and novel treatment methods using adventitia-35 transport as a pathway, while also providing a new perspective for studying the pathogenic mechanisms and diagnostic and treatment methods of AD or cerebrovascular diseases.

This application relates to the field of in vitro diagnostics and microbial detection technology, and in particular to a method and kit for optimizing reaction reagents for vaginal flora detection. The method includes serial dilution of 2×One-Step SYBR Green reagent and iScript reverse transcriptase in an RT-qPCR system, and supplementing the diluent with 0.2 mmol / L dNTPs and 1 mmol / L Mg. 2+ Achieving the reaction of 10 in an 8 μL reaction system 3 -10 8 Stable amplification of cellular equivalent RNA. This application reduces reagent usage to one-third of the original protocol while maintaining amplification efficiency, sensitivity, and reproducibility, significantly lowering detection costs and making it suitable for high-throughput screening and epidemiological studies.

The invention relates to the technical field of biological virus detection, in particular to a primer, a probe and a kit for constant-temperature fluorescence rapid detection of mycoplasma bovis. Based on a multi-enzyme constant-temperature rapid amplification technology and in combination with real-time fluorescence detection, the constant-temperature fluorescence rapid detection kit is established, the reaction is completed within 20 minutes under the constant-temperature condition of 39 DEG C, the sensitivity reaches 0.1 fg / mu L, standard strains of escherichia coli, salmonella and common golden yellow coccus and streptococcus and escherichia coli separated from cattle farms are not detected, and the specificity is high; the positive plasmid sample is repeatedly detected for 6 times, the results are consistent, and the coefficient of variation is 2.93%. The kit can be applied to rapid diagnosis and on-site monitoring of mycoplasma bovis, and provides technical support for epidemic situation control and epidemiological research.

The present application relates to the technical field of nucleic acid detection, and discloses a general primer pair for detecting the genus of Segniliparus and application thereof. The detection primer pair and identification method provided by the present application are applicable to all species of the genus of Segniliparus, including but not limited to Segniliparus rugosus, and especially unnamed ANI species. The amplification method provided by the present application has low requirements for templates, and is applicable to clinical original samples such as sputum, alveolar lavage fluid, pleural and peritoneal fluid, culture, and environmental original samples such as water, soil and air, and extracted nucleic acid, and does not depend on culture. The method is fast and low-cost, and has low requirements for equipment and detection personnel, and is applicable to laboratories at all levels. The detection reagent based on the present application can be widely applied to related fields such as environmental monitoring, clinical precise detection and epidemiological research.

The present invention relates to a highly sensitive nested multiplex PCR-based primer panel for the detection of Lumpy Skin Disease Virus (LSDV) in a biological sample obtained from bovine species and livestock animals In particular, the present invention provides a nested multiplex detection primer panel and a kit comprising the same, for the detection of Lumpy Skin Disease Virus (LSDV) and its variants thereof. The present invention also provides a multiplex primer panel for the PCR whole genome amplification and genotyping of Lumpy Skin Disease Virus (LSDV) to obtain genomic data, which can be useful in tracking the evolution of the Lumpy Skin Disease virus and correlating with disease severity. Therefore, the present invention emphasizes its utility in veterinary diagnostics, epidemiological studies, and disease management strategies related to vaccine development for Lumpy Skin Disease Virus (LSDV) infections in livestock.

The invention relates to the technical field of nucleic acid detection, and discloses a universal primer pair for detecting stenotrophomonas and application of the universal primer pair. The detection primer pair and the identification method provided by the invention are generally used for all strains of stenotrophomonas, including but not limited to stenotrophomonas maltophilia, especially unnamed ANI strains. The amplification method provided by the invention has low requirements on a template, is suitable for clinical original samples such as sputum, alveolar lavage fluid, hydrothorax and ascites, culture and the like, environment original samples such as water samples, soil, air and the like and extracted nucleic acid, and does not depend on culture. The method is rapid and low in cost, has low requirements on equipment and detection personnel, and is applicable to all levels of laboratories. The detection reagent based on the invention can be widely applied to the related fields of environmental monitoring, clinical precise detection, epidemiological research and the like.

1. The name of the design product: investigation board (for epidemiology and health statistics). 2. The use of the design product: for data collection, organization and display in epidemiological research and public health statistics. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.