The invention discloses a PCR kit for detecting ST251-type
aeromonas hydrophila and application. The kit comprises a PCR Premix, a primer pair, a standard positive
DNA template, a standard negative
DNA template and sterilization double
distilled water. An upstream primer sequence is 5'-GCGGCGTAAGCGGATTTGTAGC-3', a downstream primer sequence is 5'-CCGGTCGGATTGGTCAGGTTCAT-3', the annealing temperature is 63 DEG C, a correct amplification product is 826bp in size, the standard positive
DNA template is
genomic DNA extract of an ST251-type
aeromonas hydrophila XS91-4-1 strain, and the
DNA concentration is 500 nanograms per microliter; the negative
DNA template is genomic extractive of an
aeromonas veronii IB340 strain, and the
DNA concentration is 500 nanograms per microliter. The kit is applicable to all kinds of
gene amplification instruments, high in detection specificity and sensitivity, quick, accurate and good in stability. The kit can be used for quickly diagnosing
freshwater fish outbreak diseases and blood poisoning caused by
aeromonas hydrophila of other fishes and quickly detecting and identifying
bacteria and then is widely applied to
disease prediction and forecast and epidemiologic studies.