38 results about "Epidemiologic study" patented technology

Methods and apparatus for improving measurements of cardiovascular health status in a given individual are provided. The comprehensive assessment of cardiovascular health includes at least two components: risk factor assessment based on epidemiologic studies and functional status of the individual. Structural studies of the individual can also be included in the comprehensive assessment of cardiovascular health. The invention aims to improve detection, treatment, devices, and administration of cardiovascular risk assessment.

The invention discloses a hybridoma cell strain 6D6 of a specificity monoclonal antibody capable of continuously and stably secreting foot-and-mouth disease resistant O-type (O/GX/09-7) viruses, and the specificity monoclonal antibody secreted by the hybridoma cell strain. The 6D6anti can recognize the foot-and-mouth disease O-type (O/GX/09-7) viruses in a specificity mode, the 6D6anti is used as a coating and an enzyme labeled antibody in ELISA detection and can be used for detecting the foot-and-mouth disease O-type (O/GX/09-7) viruses, and the advantages of high specificity and high sensitivity are achieved; the hybridoma cell strain, the antibody and the application can play an important role in detection of the foot-and-mouth disease O-type (O/GX/09-7) viruses, vaccine production and epidemiologic study.

The invention discloses a primer combination used for detecting SARS-CoV-2 whole genome. The primer combination comprises specific primers used for SARS-CoV-2RNA reverse transcription and a primer group for amplification of the whole genome; by adoption of the primer combination, after target genes are amplified by reverse transcription and nest type PCR, on the basis of a next-generation semiconductor sequencing platform, a next-generation semiconductor sequencing technology used for detecting the SARS-CoV-2 whole genome and mutation sites is constructed; the method has the advantages of being wide in applicability, high in flux and good in accuracy, and can detect multiple samples one time; the convenient method is provided for deep analysis of the SARS-CoV-2 genome; and the method has important meanings for epidemiologic study, clinical early molecular diagnosis and the like.

The invention discloses a malaria serum with an anti-tumor function and a preparation method and application of the malaria serum with the anti-tumor function.According to long-term arduous scientific experiments, the serum of patients with malaria has a certain anti-tumor function, and particularly the serum of patients with a syndrome of malaria and thrombocytopenia is effective in treatment or prevention of tumors, especially liver cancers, under specific concentration conditions.According to experiments, the serum has an evident apoptosis induction function on hepatoma cell strains (7721) cultured in vitro, and the hepatoma cell apoptosis induction ratio is increased along with increase of serum concentration.The malaria serum with the anti-tumor function and the preparation method and application of the malaria serum with the anti-tumor function provide theoretical and experimental bases for further researches on tumor cell apoptosis induction factors generated by plasmodia, breaks a new path for rediscovery pathogenesis of critical and cerebral malaria cases and epidemiologic researches of various plasmodium species and strains, and make new effort in application of the malaria serum or preparations thereof to preparation of medicines for treatment or prevention of the tumors, thereby having great scientific research values and significances.

The invention discloses a method for detecting canine distemper virus by using a one-step reverse transcription-polymerase chain reaction (RT-PCR) method. The method comprises the following steps of: preprocessing a sample for the RT-PCR detection; extracting total ribonucleic acid (RNA); obtaining a specific amplification product by one-step RT-PCR reaction; and performing 1 percent agarose gel electrophoresis identification on the amplification product. The method of the invention has the characteristics of high flexibility, high specificity, simple and convenient operation procedure, low contamination rate and reliable detection result; the acquired sequence information is directly used for analyzing genetic derivation relation of an epidemic strain; and the method is suitable for etiological diagnosis and epidemiological research of canine distemper.

The invention discloses a PCR kit for detecting ST251-type aeromonas hydrophila and application. The kit comprises a PCR Premix, a primer pair, a standard positive DNA template, a standard negative DNA template and sterilization double distilled water. An upstream primer sequence is 5'-GCGGCGTAAGCGGATTTGTAGC-3', a downstream primer sequence is 5'-CCGGTCGGATTGGTCAGGTTCAT-3', the annealing temperature is 63 DEG C, a correct amplification product is 826bp in size, the standard positive DNA template is genomic DNA extract of an ST251-type aeromonas hydrophila XS91-4-1 strain, and the DNA concentration is 500 nanograms per microliter; the negative DNA template is genomic extractive of an aeromonas veronii IB340 strain, and the DNA concentration is 500 nanograms per microliter. The kit is applicable to all kinds of gene amplification instruments, high in detection specificity and sensitivity, quick, accurate and good in stability. The kit can be used for quickly diagnosing freshwater fish outbreak diseases and blood poisoning caused by aeromonas hydrophila of other fishes and quickly detecting and identifying bacteria and then is widely applied to disease prediction and forecast and epidemiologic studies.

The invention is to provide a method and kit based on recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) for detection of caprine arthritis-encephalitis virus (CAEV) infection. The method and kit are suitable for both laboratory and field application, and are specific and sensitive for detecting CAEV proviral DNA in goats in a fast manner. The method and lit of the invention are also applicable for on-site utilization at farms and should be useful in both eradication programs and epidemiological studies.

The invention discloses a method for simultaneously detecting three groups of rotaviruses A, B and C, and belongs to the field of biological detection. A pair of specific primers and a specific probewith different fluorescent labels are designed for target genes of group A rotavirus, group B rotavirus and group C rotavirus, a pair of universal primers is further designed, and during PCR amplification, the specific primers with universal primer tags are used for enrichment and amplification; and then universal primer tags are used for exponential amplification, and fluorescence signals of exponential amplification products are collected for detection. The method can simultaneously detect the group A rotavirus, the group B rotavirus, and the group C rotavirus. The method is high in specificity, high in sensitivity and short in detection time, and can be used for early rapid diagnosis of rotavirus infection and epidemiological study of the rotavirus.

A monoclonal antibody for H1N1 swine influenza A virus hemagglutinin protein and a double-antibody sandwich ELISA kit are disclosed. The kit comprises a solid phase vector coated with the monoclonal antibody, a rabbit polyclonal antibody labeled by horseradish peroxidase, an H1N1 hemagglutinin protein standard substance, a sample diluting liquid, a washing liquid, a substrate coloring liquid and a reaction terminating liquid. The kit is good in sensitivity, capable of performing quantitative detection for the swine influenza virus hemagglutinin protein, and specifically recognizing main prevalent strains of A(H1N1) influenza outbreak in 2009, and free of cross reactions with other strains of the H1N1 influenza viruses and other influenza virus subtypes. The kit is simple to operate, can be used for supporting fundamental research of the H1N1 swine influenza virus, and has important significance for performing epidemiologic study on the H1N1 influenza viruses in different zones and different years.

The invention provides a related gene, method, primer group and kit for identifying mycobacterium tuberculosis complex flora and detecting drug resistance. According to the related gene, method, primer group and kit, not only can the mycobacterium tuberculosis complex flora be identified, but also 16 common anti-tuberculosis first-line and second-line drug resistance gene mutations of the mycobacterium tuberculosis complex flora can be distinguished and detected at high resolution, and rapid, simultaneous and accurate drug resistance detection is achieved. The related gene, method, primer group and kit can be used for clinical treatment reference and epidemiological research.

Device, method, and system for nanoparticle capture, tracking, and/or detection. A functional paper-based platform is modified with capture ligands to create binding sites for nanoparticles. According to an embodiment, nanoparticle binding produces visual images of the particle content and distribution on the modified sensing surface, which provides capabilities for both NP sequestration and real-time detection. According to an embodiment the system may be utilized for environmental decontamination, fabrication of personal protective equipment, field monitoring, and epidemiological studies. The availability of inexpensive and easy-to-use quantitative methods can facilitate rapid assessment and measurement of NPs concentration and the level of exposure for large scale toxicological and epidemiological testing

The invention provides a human enterovirus71-type C4-subtype lethal strain SD095 with the accession number of CGMCC (China General Microbiological Culture Collection Center) NO.6601 and its application. A rat adaptive lethal strain is acquired by conventional virus isolation cultivation, animal infection and a series of technical means, and an animal lethal model based on the virus strain SD095 can be established. The acquisition of the lethal virus strain and the establishment of the animal lethal model have important significance for promoting clinical treatment and researches in China on diseases and death cases caused by EV71 (human enterovirus 71) virus, and have important values for further promotion of experimental and clinical studies and epidemiologic studies on the EV71, and new vaccine development and evaluation.

The invention discloses an epidemiological research population screening method based on medical big data and a storage medium. The method comprises the following steps: acquiring health medical data; determining to-be-constructed target population types according to requirements of epidemiological research, wherein the to-be-constructed target population types comprise a cross section research population, a fixed queue research population and a dynamic queue research population; setting screening conditions including a research time range, follow-up visit time, a time window, an inclusion rule and an ending rule; and screening records meeting the requirements of the target crowd from the health medical data, and constructing the target population. According to the medical big data-based epidemiological research population screening method and the storage medium, the target population is directly screened from the existing massive medical data to be brought into epidemiological research, and the target population comprises a cross section research population, a fixed queue research population and a dynamic array research population; the efficiency and correctness of epidemiological research population screening based on medical big data are improved, and manpower is saved.

The invention belongs to the technical field of oxidative stress biomarker detection, in particular to a method for testing oxidative stress biomarkers in blood plasma with a low coefficient of variation. The method comprises the following steps: taking a mixture of blood plasma and an extractant with the mixed volume ratio of 1: 20 as a to-be-detected substance solution, taking a supernatant of the to-be-detected substance solution and carrying out fluorescence determination by adopting a fluorescence microplate reader under the conditions of three excitation or emission wavelengths. According to the method, the coefficient of variation of oxidative stress biomarkers in blood plasma is remarkably reduced, the inter-batch coefficient of variation of FlOPs is reduced to be less than 3.6%, and the intra-batch coefficient of variation is reduced to be less than 2.7%, so that a more reliable FlOPs measurement method is provided for future epidemiological research and potential clinical practice.

The invention discloses a cognition behavior intervention system for depression of a senile dementia family caregiver. The intervention comprises the components of a caregiver training module which isused for training professional nurses so that the professional nurses master an intervention program, an intervention method and a therapeutic communication skill; an intervention pointing object module which is used for screening the caregiver with a depression symptom; an intervention content module which is used for performing integrated cognition behavior intervention according to the characteristics of the Chinese local senile dementia family caregiver; an intervention time length module which is used for determining an intervention time length of five hours, a face-to-face interventiontime length of 1 hour in each month and a telephone follow-up intervention time length of 20-30 minutes; an intervention interval module which is used for face-to-face intervention in a frequency of one time in each month, telephone follow-up surveying intervention in a frequency of one time in each month, wherein the interval time between the face-to-face intervention and the telephone follow-upsurveying intervention is two weeks; and an intervention form module which is used for performing the face-to-face intervention and the telephone intervention. Ten centers for epidemiologic studies depression scale are used for testing and screening the caregiver who accords with a preset depression standard, thereby realizing a purpose of mitigating or alleviating depression of the caregiver.

The invention discloses fluorescent RT-PCR primers and a fluorescent probe for detecting bovine torovirus. The primers and the fluorescent probe include a pair of specific forward and reverse primers and a fluorescent probe which are designed in accordance with the conserved sequence of an M gene of the bovine torovirus. The invention also discloses a kit and a detection method for detecting the bovine torovirus. The pair of specific probes and primers are designed are in accordance with the conserved sequence of the gene of the bovine torovirus; it can make a definite diagnosis that a sample is infected with the bovine torovirus when a result of PCR detection is positive; the probe and the primers have the characteristics of being convenient and rapid, high in specificity and high in sensitivity; and the probe and the primers are applicable to the rapid diagnosis of the bovine torovirus in living cattle and cattle meat quarantine as well as to epidemiology studies.