18 results about "Epidemiologic study" patented technology

The invention relates to the technical field of artificial intelligence prediction and evaluation, and discloses a Mendel randomization evaluation system and method for risk of social activities to breast cancer, the system comprises a data acquisition module, a data processing module, a double-sample Mendel randomization analysis module and a result output module; the data acquisition module serves as an information source of the whole system, has the capability of accurately collecting data from a diversified and high-quality data source, and can retrieve genetic polymorphic data related to social isolation and interaction from a public or private database. According to the system and method for evaluating the risk of the social activity on the breast cancer through Mendel randomization, by integrating multi-source genetic data and applying a double-sample Mendel randomization analysis technology, the problems of confounding factors and inherent deviation in traditional epidemiological research are solved, and the risk of the social activity on the breast cancer is evaluated. According to the system, the potential causal relationship between the breast cancer and social activities such as social isolation and social interaction can be accurately evaluated, and a reliable analysis result is provided for a user.

The application discloses a triple fluorescent quantitative PCR detection kit for simultaneously detecting CHIKV, BFV and RRV, which contains a specific primer probe composition for simultaneously detecting chikungunya virus, Barmah Forest virus and Ross River virus, can simultaneously detect three viruses which are transmitted by insect vectors and cause acute fever with arthralgia symptoms in human beings in one PCR reaction tube, and has excellent specificity and repeatability, and is suitable for routine laboratory diagnosis of suspected samples of alphavirus infection, and provides a rapid and accurate detection method for the epidemiological study of CHIKV, BFV and RRV.

The invention relates to the technical field of medical animal model construction, in particular to a construction method and application of a cardiomyopathy animal model induced by a circulating system. According to the invention, the Abeta25-35 is injected into the adventitia, so that the animal model of the cardiomyopathy induced by the Abeta25-35 through the tissue fluid circulatory system of the adventitia is constructed in the SD rat for the first time. In the model, A beta 25-35 is subjected to myocardial perfusion through lower limb vascular adventitia to induce myocardial diffuse fibrosis and ejection fraction reduction of SD rats, which are in accordance with epidemiological research data. The model can be used for subsequently researching the pathogenic mechanism of A beta25-35 induced cardiomyopathy and a novel treatment method taking vascular adventitia tissue fluid transmission as a way, and also provides a brand new view angle for researching the pathogenic mechanism of AD or cerebrovascular diseases and a diagnosis and treatment method.

The application discloses a kind of detection group of triple RT-qPCR primers of group A porcine rotavirus G4, G5 and G9 strain, kit and its application, belong to molecular biology field.Primer group includes primer and probe, nucleotide sequence is as shown in SEQ ID NO.1-SEQ ID NO.8.The application can realize the simultaneous detection of group A porcine rotavirus G4, G5 and G9 genotype strain in one PCR reaction tube, and has good specificity, sensitivity and repeatability, and can be directly applied to the laboratory diagnosis of pig clinical diarrhea sample pathogen, provides a kind of fast, accurate detection method for the epidemiological study and clinical differential diagnosis of group A porcine rotavirus G4, G5 and G9 genotype, especially suitable for scientific research and clinical application, with good commercial application value.

The invention discloses a triple fluorescent quantitative PCR detection kit for simultaneously detecting CHIKV, BFV and RRV, and the kit comprises a specific primer probe composition for simultaneously detecting chikungunya virus, Bama forest virus and Rosc river virus, three viruses which are transmitted through entomophilous and cause human acute fever with arthralgia symptoms can be simultaneously detected in one PCR reaction tube, the lowest detection concentration of CHIKV plasmid samples is 20 copies / microliter, the lowest detection concentration of RRV and BFV positive samples is 10 copies / microliter, and the kit has excellent specificity and repeatability, and can be used for detecting the acute fever with arthralgia symptoms of the human acute fever with the arthralgia symptoms of the human acute fever with the arthralgia symptoms of the human acute fever with the arthralgia symptoms. The kit is suitable for conventional laboratory diagnosis of suspected alphavirus infection samples, and a rapid and accurate detection method is provided for epidemiological research of CHIKV, BFV and RRV.

The invention discloses a HIV (human immunodeficiency virus), HBV (hepatitis B virus), HCV (hepatitis C virus) and TP (total protein) hypersensitive detection kit and detection method based on a digital PCR (polymerase chain reaction) platform. The kit comprises a primer and a probe, the design of the primer and the probe is optimized, and the molecular detection system has higher sensitivity by utilizing high affinity and strong mismatch recognition capability of the LNA probe, so that a single copy can be detected. The method possibly has huge potential in the aspects of large-scale screening and clinical diagnosis of blood donors and epidemiological research of pathogens.

The invention discloses a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) primer group for detecting G4, G5 and G9 strains of group A porcine rotaviruses, a kit and application of the triple RT-qPCR primer group and the kit, and belongs to the field of molecular biology. The primer group comprises a primer and a probe, and nucleotide sequences of the primer and the probe are shown as SEQ ID NO.1-SEQ ID NO.8. According to the present invention, the group A porcine rotavirus G4, G5 and G9 genotype strains can be simultaneously detected in the same PCR reaction tube, and the kit has characteristics of good specificity, good sensitivity and good repeatability, and can be directly used for the laboratory diagnosis of pig clinical diarrhea sample pathogens, the invention provides a rapid and accurate detection method for epidemiological research and clinical differential diagnosis of G4, G5 and G9 genotypes of group A porcine rotaviruses, is especially suitable for scientific research and clinical application, and has good commercial application value.

This invention relates to the field of medical animal model construction technology, and in particular to a method for constructing an animal model of cardiomyopathy induced by the circulatory system and its application. This invention, through adventitia-35 injection, establishes for the first time an animal model of "Aβ25-35-induced cardiomyopathy via the adventitia-35 circulatory system" in SD rats. In this model, Aβ25-35 perfusion via the adventitia-35 myocardium of the lower limbs induces diffuse myocardial fibrosis and a decrease in ejection fraction in SD rats, consistent with epidemiological data. This model can be used to further study the pathogenic mechanism of Aβ25-35-induced cardiomyopathy and novel treatment methods using adventitia-35 transport as a pathway, while also providing a new perspective for studying the pathogenic mechanisms and diagnostic and treatment methods of AD or cerebrovascular diseases.

This application relates to the field of in vitro diagnostics and microbial detection technology, and in particular to a method and kit for optimizing reaction reagents for vaginal flora detection. The method includes serial dilution of 2×One-Step SYBR Green reagent and iScript reverse transcriptase in an RT-qPCR system, and supplementing the diluent with 0.2 mmol / L dNTPs and 1 mmol / L Mg. 2+ Achieving the reaction of 10 in an 8 μL reaction system 3 -10 8 Stable amplification of cellular equivalent RNA. This application reduces reagent usage to one-third of the original protocol while maintaining amplification efficiency, sensitivity, and reproducibility, significantly lowering detection costs and making it suitable for high-throughput screening and epidemiological studies.

The invention discloses RPA (Recombinase Polymerase Amplification) primers for synchronously detecting Elizabingia milieri, a virulence gene and a sulfonamide drug-resistant gene and application of the RPA primers. The sequences of the RPA primers are respectively shown as SEQ ID NO.1-6. The invention further discloses a kit for synchronously detecting the Elizabingia milieri and the virulence gene. According to the invention, RPA isothermal amplification is combined with agarose electrophoresis, so that Elizabethria milieri, a virulence gene (fepA) and a sulfanilamide drug-resistant gene (sul2) can be synchronously identified in one reaction within a short time, and the sizes of amplification products are respectively 110bp, 766bp and 243bp. The primer is high in conservative property in species, free of cross reaction among species, free of complex instruments and suitable for clinical rapid diagnosis, drug-resistant gene monitoring and epidemiological research.

The invention relates to the technical field of biological virus detection, in particular to a primer, a probe and a kit for constant-temperature fluorescence rapid detection of mycoplasma bovis. Based on a multi-enzyme constant-temperature rapid amplification technology and in combination with real-time fluorescence detection, the constant-temperature fluorescence rapid detection kit is established, the reaction is completed within 20 minutes under the constant-temperature condition of 39 DEG C, the sensitivity reaches 0.1 fg / mu L, standard strains of escherichia coli, salmonella and common golden yellow coccus and streptococcus and escherichia coli separated from cattle farms are not detected, and the specificity is high; the positive plasmid sample is repeatedly detected for 6 times, the results are consistent, and the coefficient of variation is 2.93%. The kit can be applied to rapid diagnosis and on-site monitoring of mycoplasma bovis, and provides technical support for epidemic situation control and epidemiological research.

The invention relates to a primer group, a method and a kit for detecting vibrio parahaemolyticus. The multiplex fluorescent quantitative PCR kit for detecting the vibrio parahaemolyticus comprises a primer group, a probe group and a PCR amplification premix solution. Wherein the primer group comprises at least one of specific genes gyrB and DAADH of vibrio parahaemolyticus and at least one of virulence genes TDH, T3SS1-VopS and T3SS2-VopT of vibrio parahaemolyticus, and the proportion of primers to probes in a PCR (Polymerase Chain Reaction) system is optimized. When the kit disclosed by the invention is used for detecting vibrio parahaemolyticus, microbial isolated culture and identification are not needed, so that the detection time is shortened, the detection efficiency is improved, meanwhile, the accuracy, sensitivity, stability and specificity of detection are greatly improved, and the kit has huge advantages in clinical specimen detection and epidemiological research.

The present application relates to the technical field of nucleic acid detection, and discloses a general primer pair for detecting the genus of Segniliparus and application thereof. The detection primer pair and identification method provided by the present application are applicable to all species of the genus of Segniliparus, including but not limited to Segniliparus rugosus, and especially unnamed ANI species. The amplification method provided by the present application has low requirements for templates, and is applicable to clinical original samples such as sputum, alveolar lavage fluid, pleural and peritoneal fluid, culture, and environmental original samples such as water, soil and air, and extracted nucleic acid, and does not depend on culture. The method is fast and low-cost, and has low requirements for equipment and detection personnel, and is applicable to laboratories at all levels. The detection reagent based on the present application can be widely applied to related fields such as environmental monitoring, clinical precise detection and epidemiological research.

The present invention relates to a highly sensitive nested multiplex PCR-based primer panel for the detection of Lumpy Skin Disease Virus (LSDV) in a biological sample obtained from bovine species and livestock animals In particular, the present invention provides a nested multiplex detection primer panel and a kit comprising the same, for the detection of Lumpy Skin Disease Virus (LSDV) and its variants thereof. The present invention also provides a multiplex primer panel for the PCR whole genome amplification and genotyping of Lumpy Skin Disease Virus (LSDV) to obtain genomic data, which can be useful in tracking the evolution of the Lumpy Skin Disease virus and correlating with disease severity. Therefore, the present invention emphasizes its utility in veterinary diagnostics, epidemiological studies, and disease management strategies related to vaccine development for Lumpy Skin Disease Virus (LSDV) infections in livestock.

The invention relates to the technical field of nucleic acid detection, and discloses a universal primer pair for detecting stenotrophomonas and application of the universal primer pair. The detection primer pair and the identification method provided by the invention are generally used for all strains of stenotrophomonas, including but not limited to stenotrophomonas maltophilia, especially unnamed ANI strains. The amplification method provided by the invention has low requirements on a template, is suitable for clinical original samples such as sputum, alveolar lavage fluid, hydrothorax and ascites, culture and the like, environment original samples such as water samples, soil, air and the like and extracted nucleic acid, and does not depend on culture. The method is rapid and low in cost, has low requirements on equipment and detection personnel, and is applicable to all levels of laboratories. The detection reagent based on the invention can be widely applied to the related fields of environmental monitoring, clinical precise detection, epidemiological research and the like.

The invention discloses an intestinal microbiological marker for diagnosing subtype infection of cystozoon and predicting load and application of the intestinal microbiological marker. According to the method, metagenome sequencing is carried out on microbial components in excrement of a cystoprotozoa infected person to obtain abundance of microorganisms, 22 specific strains are found through screening operation to serve as markers, a cystoprotozoa subtype infection risk prediction model is constructed, prediction sensitivity is high, specificity is good, and the accuracy is 90% or above. A detection product for detecting the abundance of the marker can be used for detecting the cystoplasma infection subtype. Furthermore, by carrying out metagenome sequencing on microbial components in excrement of a positive individual of the cystoprotozoa containing the marker, 32 microorganisms remarkably related to the cystoprotozoa loading amount are screened out, and related detection products for detecting the abundance of the intestinal microbial marker can be used for predicting the cystoprotozoa infection loading amount; therefore, a reliable detection tool is provided for epidemiological research and clinical diagnosis of the cystozoon subtype.

The present invention discloses a method for establishing an epidemiological ontology database, comprising a custom ontology database module for storing epidemiological ontology data; a database call module for loading the custom ontology database module into a survey form, setting a corresponding ontology database for a specific field, and implementing real-time call and retrieval of the custom ontology database module in the corresponding field during the survey or data input process; an instant marking module for acquiring and matching ontology data that matches the input content in real time through an array search function based on the JSON format, and using the ontology data to perform instant marking and matching on the input raw data; and a data storage management module for storing the ontology data annotated by the instant marking module in the custom ontology database module, thereby implementing standardized quality control of the ontology data. The present invention is simple to implement, efficient, and convenient for researchers to widely apply, and plays an important role in improving the quality of data standardization in epidemiological research.

1. The name of the design product: investigation board (for epidemiology and health statistics). 2. The use of the design product: for data collection, organization and display in epidemiological research and public health statistics. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.