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Malaria serum with anti-tumor function and preparation method and application of malaria serum with anti-tumor function

An anti-tumor effect, malaria technology, applied in the field of tumor treatment

Inactive Publication Date: 2016-07-13
GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, in the mid-1970s, the body of animals and humans infected with Plasmodium also produced tumor necrosis factor in foreign countries, but so far, domestic and foreign data are limited to studies on the toxicity of tumor necrosis factor produced by Plasmodium to Plasmodium There are no reports on the effects of tumor necrosis factor caused by general malaria parasites and tumor necrosis factors caused by malaria parasites that cause thrombocytopenia on various tumors

Method used

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  • Malaria serum with anti-tumor function and preparation method and application of malaria serum with anti-tumor function
  • Malaria serum with anti-tumor function and preparation method and application of malaria serum with anti-tumor function
  • Malaria serum with anti-tumor function and preparation method and application of malaria serum with anti-tumor function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Experiment of Inducing Early Apoptosis of 7721 Liver Cancer Cells by Serum from Malaria Patients

[0023] 1. Experimental materials

[0024] 1.1 Materials

[0025] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.

[0026] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.

[0027] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.

[0028] 1.2.1 Cell line: 7721 liver cancer cells were provided by Shanghai Cell.

[0029] 1.2.2 Reagents: 1640 cu...

Embodiment 2

[0044] Example 2 Serum from Malaria Patients Induced Early Apoptosis of 7901 Gastric Cancer Cells

[0045] 1. Experimental materials

[0046] 1.1 Materials

[0047] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.

[0048] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.

[0049] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.

[0050] 1.2.1 Cell line: 7901 gastric cancer cells were provided by Shanghai Cell.

[0051] 1.2.2 Reagents: 1640 culture medium: ...

Embodiment 3

[0062] Example 3 Serum from Malaria Patients Induced Jurkat Cells (Leukemia Cells) Early Apoptosis Experiment

[0063] 1. Experimental materials

[0064] 1.1 Materials

[0065] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.

[0066] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.

[0067] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.

[0068] 1.2.1 Cell line: Jurkat cells (leukemia cells) were provided by Shanghai Cell.

[0069] 1.2.2 Reagents: 1640 ...

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Abstract

The invention discloses a malaria serum with an anti-tumor function and a preparation method and application of the malaria serum with the anti-tumor function.According to long-term arduous scientific experiments, the serum of patients with malaria has a certain anti-tumor function, and particularly the serum of patients with a syndrome of malaria and thrombocytopenia is effective in treatment or prevention of tumors, especially liver cancers, under specific concentration conditions.According to experiments, the serum has an evident apoptosis induction function on hepatoma cell strains (7721) cultured in vitro, and the hepatoma cell apoptosis induction ratio is increased along with increase of serum concentration.The malaria serum with the anti-tumor function and the preparation method and application of the malaria serum with the anti-tumor function provide theoretical and experimental bases for further researches on tumor cell apoptosis induction factors generated by plasmodia, breaks a new path for rediscovery pathogenesis of critical and cerebral malaria cases and epidemiologic researches of various plasmodium species and strains, and make new effort in application of the malaria serum or preparations thereof to preparation of medicines for treatment or prevention of the tumors, thereby having great scientific research values and significances.

Description

technical field [0001] The invention belongs to the technical field of tumor treatment, and in particular relates to a malaria serum with anti-tumor effect and its preparation method and application. Background technique [0002] In 1927, the Nobel Prize in Medicine was awarded to Austrian psychiatrist Julius Wagner-Jauregg (1857-1940) for his invention of artificially infecting patients with General Paresis of the Insane GPI with Plasmodium. He was cured and relieved of the high fever caused by the patients who were helpless in the treatment of GPI diseases at that time. Therefore, he was called "the father of fever therapy" by the academic circles. Before the 1940s, "fever therapy" was widely used in the treatment of various difficult diseases at that time, such as tumors, syphilis and gonorrhea. It is still used in the treatment of tumors, and various physical heating methods have been developed to treat tumors, such as microwave thermal therapy and nano-thermal therapy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61P35/00
CPCA61K35/16Y02A50/30
Inventor 黄亚铭黄鼎铭邹春燕韦海艳黄超李黄羿林康明毛玮蒋智华黎军韦树娇张伟尉燕慧区德锦贺颖
Owner GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR DISEASE CONTROL & PREVENTION