Malaria serum with anti-tumor function and preparation method and application of malaria serum with anti-tumor function
An anti-tumor effect, malaria technology, applied in the field of tumor treatment
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Embodiment 1
[0022] Example 1 Experiment of Inducing Early Apoptosis of 7721 Liver Cancer Cells by Serum from Malaria Patients
[0023] 1. Experimental materials
[0024] 1.1 Materials
[0025] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.
[0026] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.
[0027] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.
[0028] 1.2.1 Cell line: 7721 liver cancer cells were provided by Shanghai Cell.
[0029] 1.2.2 Reagents: 1640 cu...
Embodiment 2
[0044] Example 2 Serum from Malaria Patients Induced Early Apoptosis of 7901 Gastric Cancer Cells
[0045] 1. Experimental materials
[0046] 1.1 Materials
[0047] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.
[0048] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.
[0049] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.
[0050] 1.2.1 Cell line: 7901 gastric cancer cells were provided by Shanghai Cell.
[0051] 1.2.2 Reagents: 1640 culture medium: ...
Embodiment 3
[0062] Example 3 Serum from Malaria Patients Induced Jurkat Cells (Leukemia Cells) Early Apoptosis Experiment
[0063] 1. Experimental materials
[0064] 1.1 Materials
[0065] 1.1.1 Collection of serum from malaria patients: extract whole blood from hospitalized malaria patients (including patients infected with different types of Plasmodium) accompanied by thrombocytopenia in blood routine examination.
[0066] 1.1.2 Separation and storage of serum from malaria patients: after obtaining whole blood from malaria patients, centrifuge within a short time to sort and aliquot the serum and store it at low temperature for later use.
[0067] 1.1.3 Malaria patient serum preparation before the experiment: before the experiment, the malaria patient serum stored at low temperature was taken out and warmed up at room temperature before being used for the experiment.
[0068] 1.2.1 Cell line: Jurkat cells (leukemia cells) were provided by Shanghai Cell.
[0069] 1.2.2 Reagents: 1640 ...
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