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Application of rust rot disease ginseng extract in preparation of medicine for treating tumor

A technology of ginseng extraction and rust rot, which is applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of no research report on the anti-cancer effect of ginseng in patients with rust rot, and achieve reduced survival rate and strong anti-cancer activity. Effect

Active Publication Date: 2021-08-06
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the anticancer effect of ginseng with rust-rot disease

Method used

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  • Application of rust rot disease ginseng extract in preparation of medicine for treating tumor
  • Application of rust rot disease ginseng extract in preparation of medicine for treating tumor
  • Application of rust rot disease ginseng extract in preparation of medicine for treating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 rust rot disease ginseng extract

[0044] First, weigh 75.6g of ginseng skin from rust-rot patients, wash and chop it; then add 300mL of 95% ethanol to a 500mL round bottom flask, and use Soxhlet extraction method to reflux at 110°C for 6h; finally, extract The solution was concentrated under reduced pressure and freeze-dried using a freeze dryer to obtain 6.8 g of a solid extract of rust-rot diseased ginseng.

[0045] Take 58.7g of normal ginseng skin parts, and extract by the same method as the above-mentioned ginseng extract of rust-rot patients, to obtain 5.9g of normal ginseng extract solids.

Embodiment 2

[0046] Example 2 Rust rot disease ginseng extract reduces cancer cell survival rate

[0047] Liver cancer cells in the logarithmic growth phase and normal cells HL-7702 were inoculated in 96-well plates, and the cell density per well was about 6×10 3 indivual. Dosing (the rust-rot disease ginseng extract prepared in Example 1) after the cells adhered to the wall, the test sample was set to 6 additive concentrations of the rust-rot disease ginseng extract (0, 30.75, 61.5, 125, 250 and 500 μg mL -1 ), at 37°C and 5% CO 2 Cultivate in the incubator for 48h. Then, 20 μL of MTT solution was added to each well to continue culturing. After 4 hours, the supernatant was discarded, 150 μL of DMSO solution was added to each well, and the absorbance was measured at a wavelength of 490 nm after sufficient shaking. The experiment was repeated three times.

[0048] The results showed that when the ginseng extracts of rust and rot disease were added to the culture system of cancer cells...

Embodiment 3

[0049] Example 3 Ginseng Extract from Rust and Rot Diseases Reduces the Number of Cancer Cells

[0050] Liver cancer cells in the logarithmic growth phase and normal cells HL-7702 were inoculated in 12-well plates, and the number of cells per well was about 3×10 4 indivual. After the cells adhere to the wall, add medicine (the ginseng extract of rust-rot patients and the extract of normal ginseng prepared in Example 1), and the tested samples are respectively 6 samples of ginseng extracts of patients with rust-rot and 6 normal ginseng extracts. Added concentration (respectively 0, 30.75, 61.5, 125, 250 and 500μg mL -1), at 37°C and 5% CO 2 Cultivate in the incubator for 48h. Discard the supernatant, digest with 0.25% trypsin, and count.

[0051] When the concentration of 0, 30.75, 61.5 and 125 μg mL of rust-rot disease ginseng extract and normal ginseng extract were added to the cell culture system -1 , the number of cells did not change significantly. When the concentra...

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Abstract

The invention relates to the technical field of pharmaceutical preparations, in particular to the application of rust rot disease ginseng extracts in the preparation of medicines for treating tumors. The invention provides the application of the rust rot disease ginseng extract in the preparation of medicines for treating tumors. The present invention creatively finds that the extract of ginseng with rust and rot disease has anticancer activity. Compared with the extract of normal ginseng, the extract obtained from ginseng with rust and rot disease shows stronger anticancer activity against various cancer cells. It can effectively induce the apoptosis of cancer cells and inhibit the proliferation of cancerous cells, and can be used as a broad-spectrum anti-cancer active substance to prepare anti-cancer drugs and provide a new drug solution for treating cancer.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to extracts of ginseng from rust disease and its application in preparing medicines for treating tumors. Background technique [0002] Globally, cancer morbidity and mortality are on the rise. Among various cancers, liver cancer has a high degree of malignancy and high incidence, and is considered to be one of the most common malignant tumors in the world. At present, chemotherapy and radiotherapy are the main methods for treating cancer, but patients will experience varying degrees of nausea, vomiting, fatigue, anorexia and other symptoms, which seriously affect the quality of life. [0003] Ginseng is a perennial herbaceous plant of the Araliaceae ginseng genus. It is a well-known precious Chinese medicine and rare food material at home and abroad. Ginseng has always been regarded as the king of all herbs, and has an indispensable position and role in the dev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258A61P35/00
CPCA61K36/258A61P35/00
Inventor 王作斌胡婧陈玉娟焦洁王莹高明燕卢征程杨帆胡翠华宋正勋
Owner CHANGCHUN UNIV OF SCI & TECH
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