116results about How to "The inspection method is convenient and easy" patented technology

The invention discloses a method for detecting a sulfanilamide medicine and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a sulfanilamide medicine monoclonal antibody and the sulfanilamide medicine. The sulfanilamide medicine monoclonal antibody is secreted by the hybridoma cell line SAs of a sulfanilamide monoclonal antibody, whose preservation number is CGMCC No. 3393. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the sulfanilamide medicine in products (especially milk, pork, chicken, eggs, honey, fish, shrimp and the like) eaten by animals or people. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the sulfanilamide medicine monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The invention provides an elisa kit for detecting cephalo-type medicine, containing: an ELIAS plate peridium of which is provided with peridium source, an enzyme label, cephalo-type medicine specific antibody working solution (contained when the peridium on the ELIAS plate is antigen and the enzyme label is enzyme label anti antibody or when the peridium on the ELIAS plate is anti antibody and theenzyme label is enzyme label antigen), ceftiofur standard solution, substrate developer, stop solution, concentrated cleaning solution and concentrated complex solution. The invention also disclosesa method applying the elisa kit for detecting cephalo-type medicine, including: firstly sample preprocessing is carried out, then the elisa kit is used for detection, and finally the detection result is analyzed. The elisa kit provided by the invention can be applied to detecting residual quantity of cephalo-type medicine in samples such as animal tissue (chicken, pork, fish and shrimp) and milk, etc, the operation is easy, the cost is low, the sensitivity is high, and the elisa kit is applied to on-site supervision and numerous sample screening.

The invention provides an enzyme-linked immunosorbent kit for inspecting sulfa drugs, comprising an ELISA plate which is coated with coating antigen, an enzyme label, sulfa drug specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), sulfamethoxy-isoxazole standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the sulfa drugs, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the residual amount of the sulfa drugs in animal tissues(chicken, pork, fish and shrimp), honey, eggs, milk, feeds and other samples, the operation is simple, the cost is low, the sensitivity is high and the enzyme-linked immunosorbent kit can be monitore d on-site and is applicable in screening mass samples.

The invention provides a detecting furanketone metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, furanketone metabolite specific antibody or furanketone metabolite derivative antibody, furanketone metabolite standard solution or furanketone metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting furanketone metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the furanketone metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.

The invention provides a detecting Furacilin metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, Furacilin metabolite specific antibody or Furacilin metabolite derivative antibody, Furacilin metabolite standard solution or Furacilin metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting Furacilin metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the Furacilin metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.

The enzyme-linked immunological kit for detecting 3-methyl quinoxaline-2-carboxylic acid (MQCA) as the quinoxaline medicine residue marker includes specific antibody for detecting MQCA and standard enzyme plate, standard enzyme secondary antibody and other enzyme-linked immunological agent coated with MQCA derivative and carrier protein coupler. The present invention is suitable for detecting mass samples, and has main reagent in liquid form, fast and convenient detecting process, high specificity, high sensitivity, high precision, high accuracy and other features.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting carbofuran. The ELISA kit comprises an ELISA plate coated with a carbofuran conjugated antigen, a carbofuran monoclonal antibody, an enzyme labeled antibody, a carbofuran standard solution, a substrate color development solution, a stop buffer, a cleaning solution and a re-dissolution solution. The invention further discloses a method for applying the ELISA kit to detection of the carbofuran. The method comprises the following steps: a sample is pretreated firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of the carbofuran in vegetables and tea leaves, the operation is simple and convenient, the cost is low, the sensitivity is high, on-site supervision can be realized, and the kit is suitable for screening of a large quantity of samples.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

The invention relates to a magnetic particle chemiluminescence immune assay kit of tumor marker AFP (alpha fetal protein) and a detection method of the magnetic particle chemiluminescence immune assay kit, which belongs to the technical field of the immune detection and analysis. An AFP monoclonal antibody marked by fluorescein isothiocyanate (FITC) and a monoclonal antibody marked by alkaline phosphatase (AP) are combined with an antigen to form a sandwiched immune compound of an FITC marked antibody-antigen-AP marked antibody, and the sandwiched immune compound is similar to a sandwich structure. Then the magnetic particles which are connected with the anti-FITC antibody are added, the specific binding of the anti-FITC antibody and the FITC enables antigen-antibody complex to be connected onto the magnetic particles, the magnetic particles are directly precipitated in an external magnetic field, and the compound formed in the immune reaction manner can be separated from other non-combined substances without centrifuging. According to the kit, the chemiluminescence is combined with the magnetic particles, a reaction system which is approximate to the homogenous phase is provided, and compared with the prior art, the kit has the advantages of high sensitivity, wide linear range, high speed and the like; moreover, the product cost is greatly reduced, and the application prospect on the aspects such as the clinical inspection is promising.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content ofthe porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is appl icable in screening mass samples.

The invention relates to an enzyme immune agent box for detecting ampicillin G, which comprises: enzyme mark plate which coats ampicillin G antibody or antigen, enzyme mark material, ampicillin G standard solution, base material color developing solution, compression cleaning liquid, ending solution, compression twin solution and antibody working solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The present invention discloses a method for detecting sulfaquinoxaline and its special-purpose ELIA kit. Said ELIA kit includes sulfaquinoxaline specific antibody, coating source and enzyme label. The described coating source is the conjugate of sulfaquinoxaline semiantigen and carrier protein or sulfaquinoxaline antiantibody, the described enzyme label is enzyme labeled sulfaquinoxaline antiantibody or enzyme labeled fulfaquinoxaline semiantigen. When the coating source is conjugate of the sulfaquinoxaline semiantigen and carrier protein, the described enzyme label is enzyme labeled sulfaquinoxaline antiantibody; and when the described coating source is sulfaquinoxaline antiantibody, the described cenzyme label is enzyme-labelled sulfaquinoxaline semiantigen.

The invention provides an imidacloprid detection ELISA (enzyme linked immunosorbent assay) kit which includes an ELISA plate coated with a coating antigen, a specific antibody, an enzyme marker, an imidacloprid standard solution, a substrate coloring liquid, a terminating liquid, a washing liquid and a complex solution, the coating antigen is an imidacloprid coupling antigen, and the enzyme marker is an enzyme labeled antibody. The invention also discloses an imidacloprid detection method using the ELISA kit, and the method includes first, pretreatment of a sample, then detection with the ELISA kit and final detection result analyzing. The ELISA kit can be used to detect the content of imidacloprid in vegetables and fruits, and has the advantages of being simple in operation, low in cost, high in sensitivity, capable of monitoring on site and suitable for screening a large number of samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting triadimenol. The enzyme linked immunosorbent assay kit comprises an enzyme-labeled plate coated with a triadimenol coupling antigen, a triadimenol monoclonal antibody, an enzyme-labeled antibody, a triadimenol standard solution, substrate coloring liquid, a stop solution, washing liquid and reconstitution fluid. The invention further discloses a method using the enzyme linked immunosorbent assay kit to detect triadimenol residual quantity. The method includes: preprocessing a sample, detecting with the kit, and analyzing the detection result. The enzyme linked immunosorbent assay kit can be used for detecting the residual quantity of the triadimenol in tobacco and is simple to operate, low in cost, high in sensitivity, capable of achieving on-site monitoring and suitable for the screening of a large amount of samples.

The invention discloses a method for detecting maduramicin and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a maduramicin monoclonal antibody. The maduramicin monoclonal antibody is secreted by the hybridoma cell line MAD of the maduramicin monoclonal antibody, whose preservation number is CGMCC No. 3391. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the maduramicin in animal muscles and livers. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the maduramicin monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The invention provides an enzyme linked immunosorbent assay kit detecting folic acid. The enzyme linked immunosorbent assay kit comprises an elisa plate coated with a coating antigen, a folic acid antibody working solution, an enzyme marker, a folic acid standard substance solution, a substrate developing solution, a stop solution, a scrubbing solution and a compound solution. The coating antigen is a folic acid coupling antigen, and the enzyme marker is an enzyme marking antibody. The invention further discloses a method applying the enzyme linked immunosorbent assay kit detecting the folic acid. The method comprises the steps that sample pretreatment is carried out at first, then the kit is used for detection, and finally the detection result is analyzed. The enzyme linked immunosorbent assay kit can be used for detecting the content of the folic acid in finished milk, milk powder and cereals such as rice, millet, corns, soybeans and flour. The kit is easy and convenient to operate, low in cost, high in sensitivity, capable of monitoring the folic acid on site, and suitable for screening a large number of samples.

The invention discloses a method for rapid screening the residues of antibiotics in milk-like liquid samples, including: preparation of bacillus bacteria culture used in detection, preparation of agar medium detector tube containing working bacteria and indicator, preparation of liquid culture, with the principle that antimicrobial agents can inhibit growth or propagation of bacterial sensitive to it, aspirate the liquid sample with quantitative pipet and drop in to the agar medium detector tube and add the liquid medium used for detection, in a certain temperature conditions culture vertically for a certain period of time, according to the nature and extent of color changes of solid agar medium in the tube to determine whether samples contain residues of antibiotics. Compared with the existing technology, the invention has characteristics of quick and easy, small interference, wide detection spectrum, high sensitivity, high accuracy, and good stability, applicable for high-flux and rapid detection of antibiotics residues in milk-like liquid agricultural products.

The present invention provides an enzyme-linked immunoassay kit for detecting dimethomorph. The enzyme-linked immunoassay kit contains an enzyme labeled plate coated with a dimethomorph-conjugated antigen, a dimethomorph standard substance solution, a concentrated enzyme conjugate, an enzyme conjugate working solution, a substrate chromogenic solution and a terminating solution. The invention further discloses a method for detecting dimethomorph in a sample by using the enzyme-linked immunoassay kit, wherein the method comprises: pre-treating a sample, detecting by using the kit, and analyzingthe detection result. According to the present invention, the enzyme-linked immunoassay kit can be used for detecting the residual dimethomorph in vegetable samples (raw materials, matching materialsand concentrated materials), has advantages of simple operation, low cost, high sensitivity and on-site monitoring, and is suitable for screening of a large number of samples.

The invention discloses a rapid detection method for detecting the activity of inorganic polymer cement used metakaolin. The method comprises the steps of metakaolin preparation by hydrochloric acid dissolution and calcinations, complex reaction and titration; the hydrochloric acid is the standard hydrochloric acid with the mass percentage concentration of 20%, the dissolution is carried out for 2 hours at 80 DEG C; 2-3 ml of nitroso salt indicator is added to the solution obtained after the complex reaction, 0.035 mol / L of bluestone standard volumetric solution is used for titration with the end point that the yellow indicator is changed from emerald to grass green, and the activity of the metakaolin obtained through calcinations is evaluated through calculating the dissolution rate of alumina. The detection shows that a positive correlation exists between the intensity of a hardenite and the diction method when the same metakaolin is used with a sodium silicate solution for preparing inorganic polymer cement, The method for detecting the metakaolin has the characteristics of convenience, rapidness, exactness, low cost and so on, and only three hours is required for detecting a sample.

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for detecting vitamin B12. The enzyme linked immunosorbent assay kit comprises an ELISA plate coated with a vitamin B12 coupling antigen, an enzyme labeling ant-antibody working solution, a vitamin B12 specific antibody working solution, a vitamin B12 standard substance solution, substrate developing liquid, a stop solution, a concentration scrubbing solution and redissolution liquid. The invention also discloses a method for detecting the vitamin B12 by using the enzyme linked immunosorbent assay kit. The method comprises the following steps: firstly carrying out sample pretreatment, then detecting by using the kit, and finally analyzing detection results. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of vitamin B12 in cereals (maize meal, soybean meal, millet meal and rice meal), milk and milk powder, is simple and convenient to operate, low in cost and high in sensitivity, can realize on-site monitoring and is suitable for screening lots of samples.

The invention provides an enzyme-linked immunoassay kit for detecting chlorpyrifos. The kit comprises an enzyme label board coated with chlorpyrifos coupling antigen, a chlorpyrifos monoclonal antibody, an enzyme labeling antiantibody, a chlorpyrifos standard product solution, a substrate developing solution, TMAH, a cleaning solution and a combination solution. The invention further discloses a method for detecting chlorpyrifos by means of the enzyme-linked immunoassay kit. The method includes the steps that firstly, sample pretreatment is conducted; secondly, detection is conducted by means of the kit; finally, a detection result is analyzed. The enzyme-linked immunoassay kit can be used for detecting the content of chlorpyrifos in vegetables, fruits and tea leaves, is easy to operate, low in cost, high in sensitivity, capable of achieving in-situ monitoring and suitable for screening a lot of samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting nitroimidazole drugs. The kit comprises an enzyme labeling plate coated with metronidazole coupling antigen, enzyme labeling antiantibody working solution, specific antibody working solution of the nitroimidazole drugs, metronidazole standard substance solution, substrate developing solution, stop solution, concentrated washing solution and redissolving solution. The invention further discloses a method for detecting the nitroimidazole drugs by utilizing the enzyme linked immunosorbent assay kit. The method comprises the following steps of: firstly, carrying out sample preprocessing; secondly, carrying out detection by utilizing the kit; and thirdly, analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of the nitroimidazole drugs in a honey sample, is simple and convenient to operate, is low in cost, can realize on-site monitoring and is suitable for screening of a large number of samples.

The invention relates to an enzyme immune agent box for detecting destradiol which comprises: enzyme mark plate which coats antibody or destradiol antigen, enzyme mark material, standard solution, antibody working solution, base material color developing solution, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The present invention provides an enzyme-linked immunoassay kit for chloramphenicol detection. The enzyme-linked immunoassay kit comprises an enzyme label plate coated with coating antigen, an enzyme marker, a specific chloramphenicol antibody working solution (when the enzyme label plate is coated with antigen and the enzyme marker is enzyme-labeled anti-antibody or the enzyme label plate is coated with anti-antibody and the enzyme marker is enzyme-labeled antigen, the kit contains the specific chloramphenicol antibody working solution), chloramphenicol standard solutions, a substrate coloration solution, a termination solution, a concentration washing solution and a concentration reconstituted solution. The present invention further discloses a method for detecting chloramphenicol by using the enzyme-linked immunoassay kit. The method comprises: carrying out a sample pretreatment, adopting the kit to detect, and finally analyzing a detection result. With the enzyme-linked immunoassay kit, chloramphenicol residue in animal tissues (muscles, livers and the like), delicatessen, aquatic products, royal jelly, urine, feeds, milk and other samples can be detected, characteristics of simple operation, low cost and high sensitivity are provided, and the kit and the method can be applicable for screening and on-site monitoring of a large number of samples.

The invention provides an agent box for detecting fluorine drugs of animal foodstuff, which uses enzyme immune method to detect the preprocessed animal organization, shrimp, fish and blood product. The enzyme immune agent box comprises: enzyme mark plate which coats fluorine drugs antigen, enzyme mark antibody, ciproloxacin standard solution, base material color developing solution, fluorine drugs antibody, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention provides an enzyme-linked immunosorbent assay kit for detecting diazepam. The enzyme-linked immunosorbent assay kit comprises an ELISA (Enzyme Linked Immunosorbent Assay) plate coated with a diazepam coupled antigen, a diazepam monoclonal antibody, an enzyme-labeled anti-antibody, a diazepam standard product solution, a substrate color developing solution, a stopping solution, a washing solution and a dissolution solution. The invention further discloses a method for utilizing the enzyme-linked immunosorbent assay kit to detect the diazepam. The method comprises the following steps: firstly, pre-treating a sample; secondly, detecting by utilizing the kit; finally, analyzing a detection result. The enzyme-linked immunosorbent assay kit provided by the invention can be used for detecting the content of the diazepam in animal tissues, urine, feed and water, is simple and convenient to operate, low in cost and high in sensitivity, can be monitored in field and is suitable for screening a lot of samples.

The invention discloses an enzyme immunity test agent box for testing the apurone drug, which comprises: an enzyme mark plate that coated by coat source; enzyme mark matter; apurone specific antibody working solution (when the enzyme mark plate is coated by antibody and the enzyme mark matter is the enzyme mark antibody or the enzyme mark plate is coated by the anti antibody and the enzyme mark matter is the enzyme mark antigen); apurone standard solution; base color display solution; stop solution; concentrated washing solution; and concentrated compound solution. The invention also discloses a method for using said agent box to test apurone, which comprises: processing pretreatment on the sample; then using test box to test; at last analyzing the test result. The inventive agent box can be used to test the left amount of apurone in chicken and shrimp, etc, with simple operation, lower cost, and high sensitivity.