116results about How to "The inspection method is convenient and easy" patented technology

The enzyme-linked immunological kit for detecting 3-methyl quinoxaline-2-carboxylic acid (MQCA) as the quinoxaline medicine residue marker includes specific antibody for detecting MQCA and standard enzyme plate, standard enzyme secondary antibody and other enzyme-linked immunological agent coated with MQCA derivative and carrier protein coupler. The present invention is suitable for detecting mass samples, and has main reagent in liquid form, fast and convenient detecting process, high specificity, high sensitivity, high precision, high accuracy and other features.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting carbofuran. The ELISA kit comprises an ELISA plate coated with a carbofuran conjugated antigen, a carbofuran monoclonal antibody, an enzyme labeled antibody, a carbofuran standard solution, a substrate color development solution, a stop buffer, a cleaning solution and a re-dissolution solution. The invention further discloses a method for applying the ELISA kit to detection of the carbofuran. The method comprises the following steps: a sample is pretreated firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of the carbofuran in vegetables and tea leaves, the operation is simple and convenient, the cost is low, the sensitivity is high, on-site supervision can be realized, and the kit is suitable for screening of a large quantity of samples.

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

The invention relates to a magnetic particle chemiluminescence immune assay kit of tumor marker AFP (alpha fetal protein) and a detection method of the magnetic particle chemiluminescence immune assay kit, which belongs to the technical field of the immune detection and analysis. An AFP monoclonal antibody marked by fluorescein isothiocyanate (FITC) and a monoclonal antibody marked by alkaline phosphatase (AP) are combined with an antigen to form a sandwiched immune compound of an FITC marked antibody-antigen-AP marked antibody, and the sandwiched immune compound is similar to a sandwich structure. Then the magnetic particles which are connected with the anti-FITC antibody are added, the specific binding of the anti-FITC antibody and the FITC enables antigen-antibody complex to be connected onto the magnetic particles, the magnetic particles are directly precipitated in an external magnetic field, and the compound formed in the immune reaction manner can be separated from other non-combined substances without centrifuging. According to the kit, the chemiluminescence is combined with the magnetic particles, a reaction system which is approximate to the homogenous phase is provided, and compared with the prior art, the kit has the advantages of high sensitivity, wide linear range, high speed and the like; moreover, the product cost is greatly reduced, and the application prospect on the aspects such as the clinical inspection is promising.

The invention provides an imidacloprid detection ELISA (enzyme linked immunosorbent assay) kit which includes an ELISA plate coated with a coating antigen, a specific antibody, an enzyme marker, an imidacloprid standard solution, a substrate coloring liquid, a terminating liquid, a washing liquid and a complex solution, the coating antigen is an imidacloprid coupling antigen, and the enzyme marker is an enzyme labeled antibody. The invention also discloses an imidacloprid detection method using the ELISA kit, and the method includes first, pretreatment of a sample, then detection with the ELISA kit and final detection result analyzing. The ELISA kit can be used to detect the content of imidacloprid in vegetables and fruits, and has the advantages of being simple in operation, low in cost, high in sensitivity, capable of monitoring on site and suitable for screening a large number of samples.

The invention discloses a method for detecting maduramicin and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a maduramicin monoclonal antibody. The maduramicin monoclonal antibody is secreted by the hybridoma cell line MAD of the maduramicin monoclonal antibody, whose preservation number is CGMCC No. 3391. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the maduramicin in animal muscles and livers. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the maduramicin monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The present invention provides an enzyme-linked immunoassay kit for detecting dimethomorph. The enzyme-linked immunoassay kit contains an enzyme labeled plate coated with a dimethomorph-conjugated antigen, a dimethomorph standard substance solution, a concentrated enzyme conjugate, an enzyme conjugate working solution, a substrate chromogenic solution and a terminating solution. The invention further discloses a method for detecting dimethomorph in a sample by using the enzyme-linked immunoassay kit, wherein the method comprises: pre-treating a sample, detecting by using the kit, and analyzingthe detection result. According to the present invention, the enzyme-linked immunoassay kit can be used for detecting the residual dimethomorph in vegetable samples (raw materials, matching materialsand concentrated materials), has advantages of simple operation, low cost, high sensitivity and on-site monitoring, and is suitable for screening of a large number of samples.

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for detecting vitamin B12. The enzyme linked immunosorbent assay kit comprises an ELISA plate coated with a vitamin B12 coupling antigen, an enzyme labeling ant-antibody working solution, a vitamin B12 specific antibody working solution, a vitamin B12 standard substance solution, substrate developing liquid, a stop solution, a concentration scrubbing solution and redissolution liquid. The invention also discloses a method for detecting the vitamin B12 by using the enzyme linked immunosorbent assay kit. The method comprises the following steps: firstly carrying out sample pretreatment, then detecting by using the kit, and finally analyzing detection results. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of vitamin B12 in cereals (maize meal, soybean meal, millet meal and rice meal), milk and milk powder, is simple and convenient to operate, low in cost and high in sensitivity, can realize on-site monitoring and is suitable for screening lots of samples.

The invention provides an enzyme-linked immunoassay kit for detecting chlorpyrifos. The kit comprises an enzyme label board coated with chlorpyrifos coupling antigen, a chlorpyrifos monoclonal antibody, an enzyme labeling antiantibody, a chlorpyrifos standard product solution, a substrate developing solution, TMAH, a cleaning solution and a combination solution. The invention further discloses a method for detecting chlorpyrifos by means of the enzyme-linked immunoassay kit. The method includes the steps that firstly, sample pretreatment is conducted; secondly, detection is conducted by means of the kit; finally, a detection result is analyzed. The enzyme-linked immunoassay kit can be used for detecting the content of chlorpyrifos in vegetables, fruits and tea leaves, is easy to operate, low in cost, high in sensitivity, capable of achieving in-situ monitoring and suitable for screening a lot of samples.

The invention provides an enzyme linked immunosorbent assay kit for detecting nitroimidazole drugs. The kit comprises an enzyme labeling plate coated with metronidazole coupling antigen, enzyme labeling antiantibody working solution, specific antibody working solution of the nitroimidazole drugs, metronidazole standard substance solution, substrate developing solution, stop solution, concentrated washing solution and redissolving solution. The invention further discloses a method for detecting the nitroimidazole drugs by utilizing the enzyme linked immunosorbent assay kit. The method comprises the following steps of: firstly, carrying out sample preprocessing; secondly, carrying out detection by utilizing the kit; and thirdly, analyzing a detection result. The enzyme linked immunosorbent assay kit provided by the invention can be used for detecting the content of the nitroimidazole drugs in a honey sample, is simple and convenient to operate, is low in cost, can realize on-site monitoring and is suitable for screening of a large number of samples.

The invention relates to an enzyme immune agent box for detecting destradiol which comprises: enzyme mark plate which coats antibody or destradiol antigen, enzyme mark material, standard solution, antibody working solution, base material color developing solution, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.