Method for fast filtering antibacterial medicine residue in milk-like liquid sample

Abstract

The invention discloses a method for rapid screening the residues of antibiotics in milk-like liquid samples, including: preparation of bacillus bacteria culture used in detection, preparation of agar medium detector tube containing working bacteria and indicator, preparation of liquid culture, with the principle that antimicrobial agents can inhibit growth or propagation of bacterial sensitive to it, aspirate the liquid sample with quantitative pipet and drop in to the agar medium detector tube and add the liquid medium used for detection, in a certain temperature conditions culture vertically for a certain period of time, according to the nature and extent of color changes of solid agar medium in the tube to determine whether samples contain residues of antibiotics. Compared with the existing technology, the invention has characteristics of quick and easy, small interference, wide detection spectrum, high sensitivity, high accuracy, and good stability, applicable for high-flux and rapid detection of antibiotics residues in milk-like liquid agricultural products.

Description

Technical field [0001] The invention relates to the technical field of detection and analysis of antibacterial drug residues, and specifically belongs to a method for rapidly screening antibacterial drug residues in milk liquid samples by using the growth of bacteria to be inhibited by the residual antibacterial drugs in a sample to be tested. Background technique [0002] Antibacterial drug residues have always been a serious problem plaguing food safety. Dairy farms widely use antibacterial drugs to treat various infectious diseases, which can easily cause drug residues in milk. The methods currently used to detect antibacterial drugs in milk mainly include high-performance liquid chromatography, liquid-mass spectrometry, gas-mass spectrometry, thin-layer chromatography and other chromatographic methods. The main shortcomings of these chromatographic methods are: the need for professional equipment; the sample pretreatment procedures are cumbersome; the operation process is com...

AI Technical Summary

Problems solved by technology

The main disadvantages of these chromatographic methods are: professional equipment is required; sample pretreatment procedures are cumbersome; the operation process is complicated; time-consuming; professionally trained technicians are required to operate; there are many interference factors; analytical instruments are expensive; it is difficult to popularize at the grassroots level. Generally only suitable for laboratory testing; testing costs are high

However, in the specific operation process of this method, adding the number of Streptococcus thermophilus in the milk will have a great impact on the detection results, resulting in false negative or false positive results. At the same time, the detection time is also long, which is laborious