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Enzyme-linked immunological kit for detecting quinoxaline medicine residue

An enzyme-linked immunosorbent reagent, quinoxaline-based technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to reflect olaquindox, cannot use olaquindox to monitor, and does not form a large-scale monitoring of quinoxaline drug immunity Kits and other problems, to achieve the effect of convenient inspection method, simple pretreatment process and high accuracy

Inactive Publication Date: 2007-10-03
中国农业科学院上海家畜寄生虫病研究所
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AI Technical Summary

Problems solved by technology

Therefore, it cannot reflect the true situation of olaquindox residues, and these methods cannot be used for the monitoring of olaquindox and its residue markers, and there is no immune kit that can monitor quinoxaline drug residues in large quantities.

Method used

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  • Enzyme-linked immunological kit for detecting quinoxaline medicine residue
  • Enzyme-linked immunological kit for detecting quinoxaline medicine residue
  • Enzyme-linked immunological kit for detecting quinoxaline medicine residue

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Experimental program
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Embodiment Construction

[0023] 1. The preparation of MQCA derivatives (when R is 6-CH 2 OH as an example to introduce the preparation of MQCA derivatives in detail)

[0024] a) Step 1: Dissolve 1 part of 5-hydroxymethylbenzofurazan in 2 to 6 parts of ethyl acetoacetate, and keep stirring for 6 to 8 hours in the presence of 2 to 3 parts of alkali to obtain a yellow precipitate. For 3-methyl-6-hydroxymethyl-2-ethoxycarbonylquinoxaline-1,4-dioxide. The reaction temperature is -10 to 50°C; the base used for the catalyst in the reaction can be an organic base or an inorganic base, and the organic base includes diethylamine, triethylamine, trimethylamine, n-propylamine, pyridine, picoline, piperidine, etc. Inorganic bases include sodium hydroxide, potassium hydroxide, calcium hydroxide, amine hydroxide and the like.

[0025]

[0026] b) The second step: 1 part of 3-methyl-6-hydroxymethyl-2-ethoxycarbonylquinoxaline-1,4-dioxide reacts with 3 to 6 parts of reducing agent in an appropriate amount of wate...

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Abstract

The enzyme-linked immunological kit for detecting 3-methyl quinoxaline-2-carboxylic acid (MQCA) as the quinoxaline medicine residue marker includes specific antibody for detecting MQCA and standard enzyme plate, standard enzyme secondary antibody and other enzyme-linked immunological agent coated with MQCA derivative and carrier protein coupler. The present invention is suitable for detecting mass samples, and has main reagent in liquid form, fast and convenient detecting process, high specificity, high sensitivity, high precision, high accuracy and other features.

Description

technical field [0001] The invention relates to the technical field of detection and analysis of veterinary drug residues, in particular to an enzyme immunoassay kit for detection of quinoxaline drug residues. Background technique [0002] Quinoxaline drugs are the most widely used feed drug additives in my country. They have the functions of antibacterial, growth promotion, increasing feed remuneration, improving the quality of livestock products, etc. But there is a large amount of illegal use of drugs such as carbadox. [0003] Studies have shown that quinoxaline prototype drugs and their metabolites have obvious safety problems. For example, olaquindox and its residues can produce photosensitive toxicity, adrenal cortex damage and water-salt metabolism disorders, chromosomal aberrations, genotoxicity, intestinal flora disorder, liver and kidney damage, etc.; Tract and respiratory tract mucosal bleeding, hypothermia, poor blood coagulation, liver and splenomegaly and oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577
Inventor 薛飞群张丽芳裘敏琪费陈忠张可煜晁博郑文丽
Owner 中国农业科学院上海家畜寄生虫病研究所
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