Magnetic particle-isolated chemiluminescence immunoassay for detecting Tau protein (TAU)

A technology of chemiluminescence immunity and magnetic particles, which is applied in the direction of chemiluminescence/bioluminescence, analysis of materials through chemical reactions, analysis of materials, etc., can solve complicated and cumbersome operations, low automation of ELISA methods, and large pollution of RIA methods question
CN109580955AInactive Publication Date: 2019-04-05BEIJING LEADMAN BIOCHEM
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING LEADMAN BIOCHEM
Publication Date
2019-04-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a magnetic particle-isolated chemiluminescence immunoassay for detecting Tau protein (TAU). A kit composition comprises a calibration product, a quality control product reagentA, reagent B, concentrated cleanout fluid, and luminescent substrate solution, wherein the calibration product is Tau protein (TAU) antigen in a series of concentration and used for establishing a standard curve; the quality control product is prepared from the buffer solution containing Tau protein (TAU) antigen in a series of concentration; the reagent A is the magnetic particle marked Tau protein (TAU) antibody solution in a series of concentration; the reagent B is the alkaline phosphatase marked Tau protein (TAU) antibody solution in a certain concentration; the concentrated cleanout fluid is used for preparing the cleanout fluid; the luminescent substrate solution is the alkaline phosphatase (ALP) catalyzed luminescent substrate solution. The signal intensity and sensitivity of theimmunoreactions are greatly improved, the strong chemiluminiscence signal can be produced when the low-content substance performs the immune binding, a more accurate, precise, convenient, faster and simpler method is provided for the detection of the human body Tau protein (TAU).
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Description

technical field

[0001] The invention belongs to the technical field of immune detection and analysis, and provides a magnetic separation chemiluminescence immunoassay method for detecting Tau protein (TAU), which is suitable for quantitative detection of human serum Tau protein (TAU). Background technique

[0002] Tau protein, also known as microtubule association protein (MAP), exists in the axons of neurons in normal brain tissue, and has the function of synthesizing and stabilizing the skeleton of neurons. The cellular function of Tau protein in the normal brain is to bind to tubulin to promote its polymerization to form microtubules; bind to the formed microtubules to maintain microtubule stability, reduce the dissociation of tubulin molecules, and induce microtubule bundles. The Tau protein molecule in normal brain contains 2-3 phosphate groups, while the Tau protein in the brain of Alzheimer's disease (AD) patients is abnormally hyperphosphorylated, each molecule of Ta...

Claims

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