45 results about "Porcine plasma" patented technology

A preparation method of porcine thrombin relates to a preparation method of thrombin. The preparation method of porcine thrombin is used for solving the problems of low yield and unsuitability for large-scale production of the existing preparation method of porcine thrombin. The method provided by the invention comprises the following steps of: 1, separating porcine plasma; 2, inactivating virus by a chemical method; 3, absorbing prothrombin; 4, collecting the prothrombin; 4, nano-filtering; 6, activating the prothrombin; and 7, freeze-drying and storing the prothrombin. The preparation method provided by the invention is capable of improving the biosecurity of the thrombin product, and simultaneously, obtains higher thrombin yield rate by employing a special ion exchange resin extraction process; specifically, 80000 IU / L plasma can be obtained. According to the invention, the method of directly and proportionally feeding the ion exchange resin into the plasma is adopted, one ton to several tons of plasma can be processed each time; therefore, large-scale production is realized. The preparation method of porcine thrombin is applied to the industrial production field of thrombin.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content ofthe porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is appl icable in screening mass samples.

The invention provides a preparation method for anti-lymphocyte immunoglobulin, which has the steps that human peripheral blood T lymphocytes or human thymocyte is used for immunizing healthy pigs for collecting and separating porcine plasma; after the manufacturing with 20 percent of ethanol, components I plus II plus III deposit is separated by a low-temperature high-speed centrifuge or a filter press; after the manufacturing with 14 percent of ethanol, supernatant fluid is separated and collected; after the manufacturing with 25 percent of ethanol and component II deposit is separated; subsequently, anti-T cell porcine immunoglobulin is obtained after the steps of refinement, configuration and virus inactivation of immunoglobulin, etc. Compared with the currently used ammonium sulfate precipitation method, the technology of the invention has the advantages of mass production, simple operation, easy command, short production period, little reagent usage, low cost, no pollution, and the like, which overcomes the disadvantages of the existing technology of hard realization of large-scale production, low yield, serious waste, environment pollution, high production cost, complex production process, various service chemical agents, long production period, high equipment occupation rate, etc.

The invention provides ash oil putty for an ancient architecture material and a preparation method thereof. The ash oil putty contains ash oil, a blood material and brick dust, wherein the mass ratio of the ash oil to the blood material to the brick dust is 1 to 1-3 to 5-10, the ash oil is prepared from raw tung oil, moringa seed oil, linseed oil, catalpa oil, Chinese herbal medicine volatile oil, minium powder, manganese dioxide metal drier, litharge, graphene and other raw materials. The blood material is prepared from pig plasma, quick lime water, activated carbon powder, light calcium powder, aerogel powder and hypocrellin. The brick dust is obtained by smashing oil-immersed black brick coarse granules. The ash oil, the blood material and the brick dust are mixed in proportion. The ash oil putty has good adhesion and toughness, increased tension, good coating continuity and is easy to construct. Dried ash oil putty does not crack, is easy to polish, also has excellent waterproof, anticorrosive, antibacterial and ultraviolet-proof properties, is especially suitable for repair and filling of the ancient architecture material, is especially used for ancient architecture wood materials and plays an excellent wood material filling effect.

The present invention provides a method of administering porcine B-domainless factor VIII (OBI-1) to a patient having factor VIII deficiency to provide more rapid and effective protection against bleeding episodes, compared to formerly available methods, or to provide more effective protection to such patients during non-bleeding periods. This invention is based on the discovery that the recombinant B-domainless porcine fVIII, termed OBI-1, has greater bioavailability compared to the natural porcine fVIII partially purified from porcine plasma, termed HYATE:C. Therefore, the inventive method employs lower unit doses of OBI-1, including, alternatively, omission of antibody-neutralizing dosage, or has longer intervals between the administration, compared to HYATE:C, to provide equivalent protection in patients having fVIII deficiency. The invention further provides pharmaceutical compositions and kits containing OBI-1 in combination with a pharmaceutically acceptable carrier, that are useful for treating patients in need of fVIII more effectively.

The present invention relates to a pig blood treatment method, particularly to a pig blood extracting and using method, which comprises: preparing a pig blood hemoglobin solution, wherein an additive is added to pig blood, centrifuging separation is performed to obtain the first supernatant layer and the first precipitation layer, separating is performed to obtain the first precipitate, and a first ultrasonic wave treatment is performed; pre-treating, wherein physical deoxidation is performed, the pH value is adjusted, and a second ultrasonic wave treatment is performed; carrying out sectional enzymolysis decolorization; carrying out active carbon decoloration, wherein active carbon with the weight of 1-2% of the mass of the solution is added, and a decoloration treatment is performed for 5-10 min at a temperature of 25-35 DEG C; post-treating, wherein the pH value is adjusted, and centrifuging separation is performed to obtain the second supernatant layer and the second precipitation layer; taking the second supernatant layer, and carrying out ultra-filtration concentration separation to obtain the second concentrated solution; preparing pig blood plasma protein powder, wherein the first supernatant layer is subjected to spray-drying; preparing pig hemoglobin powder, wherein the second concentrated solution is subjected to spray-drying; and preparing a heme peptide, wherein the second precipitation layer substance is taken and is subjected to roller drying. The method of the present invention has the good decolorization effect, and the product with the high protein content and the low ash content can be prepared.

The invention provides a feed for preventing ectoparasites of trachinotus ovatus. The feed comprises the following components in parts by weight: 30-35 parts of steamed fish meal, 10-20 parts of hermetia illucens insect, 8-16 parts of antarctic krill powder, 10-20 parts of soybean meal, 10-20 parts of pig plasma protein, 10-15 parts of flour, 5-10 parts of yeast extract, 6-10 parts of compound grease, 0.5-0.6 part of compound mineral, 0.1-0.2 part of compound vitamin and 0.15-0.25 part of Australian eucalyptus tereticornis plant essential oil. The feed for preventing ectoparasites of trachinotus ovatus is rich in amino acids, small peptides, high unsaturated fatty acids and other nutrients required by growth of the trachinotus ovatus, can promote rapid growth of the trachinotus ovatus, andcan effectively prevent and treat ectoparasites of the trachinotus ovatus, improve survival rate and immunity, and effectively protect animal health.

The invention discloses a culture medium achieving high-effective cultivation of flammulina velutipes through a bio-magnetic effect. The culture medium includes following components, by weight, bamboo vinegar, gelatin / hyaluronic acid composite micro-spheres being controllable in appearance, xylitol, porous silicon dioxide micro-spheres with nano Fe3O4 supported thereon, silkworm chrysalis powder, Muyu stone powder, porcine plasma protein powder, bran, corncob, peanut shell, cotton seed hull, orange residue, yeast, protease and a proper amount of magnetized water. The culture medium for flammulina velutipes is prepared from the silkworm chrysalis powder and the like materials, thereby achieving proportion of nitrogen source and carbon source in different types and further increasing the activity of lignin-degrading enzyme of flammulina velutipes. Meanwhile, by means of the porous silicon dioxide micro-spheres with the nano Fe3O4 supported thereon, the magnetized water and the composite yeast supported by the gelatin / hyaluronic acid composite micro-spheres, the influence on activity of the lignin-degrading enzyme of the flammulina velutipes is effectively improved. During the fermentation process, magnetic stirring is carried out so that the raw materials are completely decomposed and the culture medium is balanced in nutrition.

The invention relates to the technical field of food processing, in particular to a Jirem blood sausage and a making method of the Jirem blood sausage. The Jirem blood sausage is unique in flavor, high in nutritive value and convenient to eat. The Jirem blood sausage comprises corn flour, buckwheat, animal blood and seasoning, the animal blood is the blood of poultry and livestock, pig blood is adopted preferably, and by weight, the corn flour accounts for 350-450 parts, the buckwheat accounts for 600-700 parts, and the animal blood accounts for 1000-1300 parts. Compared with the prior art, the Jirem blood sausage is simple in processing technology, good in taste and capable of fully maintaining the nutritive value of blood food.

The invention provides a safety evaluation method for plasma protein peptide. According to the method, national seriously-wasted pig blood is selected as a raw material for extracting plasma protein, and pig plasma bioactive peptide is prepared by enzymolysis. Experimental study on acute toxicity and genetic toxicity in a mouse is performed for evaluating the safety of the pig plasma bioactive peptide and providing scientific proof for development and reasonable and safe use of animal blood polypeptide health-care food.

The invention relates to the technical field of feed, in particular to an antibiotic-free cured nursing material suitable for piglets. The antibiotic-free cured nursing material is characterized by comprising the following raw materials in parts by weight: 49-50 parts of a corn-soybean meal mixture, 0.2-0.3 part of methionine, 2-3 parts of fish meal, 0.9-1.1 parts of defatted rice bran, 0.9-1.2 parts of threonine, 0.2-0.3 part of tryptophan, 2-2.5 parts of fish meal, 5-6 parts of glucose, 2-3 parts of plasma protein, 2-3 parts of porcine plasma protein powder, 11-12 parts of whey powder, 2-3 parts of butyrin, 2-3 parts of cheese, 14-16 parts of rice, 0.5-0.6 part of compound vitamin, 0.5-0.6 part of organic trace element for pigs, 0.10-0.15 part of salt, 0.1-0.15 part of a mildew preventive, 0.5-1 part of a compound enzyme preparation and 0.2-0.8 part of calcium formate.

The invention discloses a method for detecting difloxacin mesylate in pig plasma and alveolar fluid. The method includes the following steps that (1) an objective compound in the pig plasma and the alveolar fluid is extracted by adopting acetonitrile, and supernatant is obtained after centrifugation; (2) blowing is conducted with nitrogen at 50 DEG C until drying, redissolution, centrifugation, and filtration are conducted, and sample extractive is obtained; and (3) a sample is fed, high performance liquid chromatography detection is conducted, a mobile phase is acetonitrile-0.05% phosphoric acid, a phase A is the acetonitrile, a phase B is the 0. 05% phosphoric acid, and gradient elution is conducted. The method for extracting the sample and detection the difloxacin mesylate in the pig plasma and the alveolar fluid is established, and the method has the characteristics of being simple, fast and high in sensitivity.

The invention discloses a method and device for extracting plasma protein powder from pig blood, and the method comprises the steps: S01, primarily filtering pig blood, S02, carrying out preliminary centrifugal separation of the filtered pig blood, S03, carrying out complete separation of the pig blood after the preliminary centrifugal separation in a separator, S04, carrying out secondary centrifugal separation of the separated pig blood, thereby obtaining the pig plasma, S05, pressurizing, drying and filtering the pig plasma, S06, drying the pressurized and dried pig plasma in a drying machine, and S07, finally drying the dried pig plasma in a finished product box to obtain a finished product. In S01, the impurities are removed, the processed pig plasma does not contain any impurity, after centrifugation, the plasma does not contain any impurity, the quality of the pig plasma is ensured; Due to S03, the plasma subjected to primary centrifugation has higher purity during secondary centrifugation; due to S05, water in plasma is reduced, and thus the plasma becomes viscous; in S06, fully dried plasma powder is obtained, and in S07 plasma powder is finally dried to obtain plasma protein powder.

The invention relates to a compound for preventing and controlling porcine virus diseases and a preparation method thereof, in particular to a compound for preventing and controlling porcine virus diseases. The compound is formed by mixing and preparing the following raw materials proportioned by weight portion: 1-5 portion(s) of porcine plasma protein powder and 1-3 portion(s) of astragutus pokysacharin. The compound has reasonable prescription and strong swine antivirus ability. The preparation method has advanced process, ensures the preparation to be stable and realizes long storage period. The drugs in the compound have extensive sources, have no side effect, difficultly generate the drug resistance and conform to the international health inspection standard. The compound is particularly suitable for treating infectious gastroenteritis diseases, atypical swine fever, rotavirus diseases, porcine epizootic diarrhea and other diseases.

The invention relates to the field of cell biology, in particular to a pig platelet lysate and a preparation method thereof. The preparation method comprises the following steps: re-suspending platelets collected from pig whole blood in pig plasma from which fibrinogen is removed through centrifugation, conducting repeated freezing and thawing, then performing centrifuging, collecting supernate, and filtering and sterilizing the supernate through a filter to obtain a filtrate, namely the pig platelet lysate. According to the invention, the platelet lysate is prepared from the pig blood, so the risk of heterologous protein residues caused by heterologous serum is reduced, and the hidden danger that unknown bovine endogenous infectious diseases possibly exist and influence cultured pig cells is reduced.

The present invention discloses a coating feed with an effect of leech yield increasing, wherein the coating feed is prepared from the following raw materials by weight: 30-40 parts of corn powder, 20-30 parts of walnut cake meal, 30-40 parts of scallop meat, 2-3 parts of fish sauce, 40-50 parts of porcine plasma, 5-8 parts of cheese, 40-50 parts of amaranthus hypochondriacus, 30-40 parts of tenebrio molitor, 5-8 parts of plasma protein powder, a proper amount of edible oil, a proper amount of yeast, a proper amount of Bacillus natto, a proper amount of sodium hydroxide, a proper amount of chitosan, and a proper amount of water. According to the present invention, the tenebrio molitor and the amaranthus hypochondriacus in the feed are subjected to extraction, centrifugation fermentation and other processes to prepare the protein peptide easily digested and absorbed by leeches, and advantages of good physiological functions, feed aroma and easy storage are provided; and the scallop meat has characteristics of delicious taste and rich nutrition, and the mixing fermentation is performed to make the feed have the attractant mellow aroma, such that the food intake of the leeches is increased, the growth and development of the leeches is promoted, and the leech yield is improved.

The invention relates to the technical field of experimental medicaments, and discloses a preparation method and application of anticoagulant pig blood and pig plasma for a thrombus test; the anticoagulant pig blood and the anticoagulant platelet-rich pig plasma prepared by the invention can replace human blood to carry out the thrombus test and simulate human bleeding and hemostasis conditions, so that the thrombus test is successfully carried out, the test cost is reduced, human blood resources can be saved, and meanwhile instrument research and development and performance testing of a thrombelastogram and a blood clotting instrument and development of some clinical drugs are facilitated.

The invention relates to an anti-aging health-care compound. The compound is prepared from the following raw materials in parts by weight: 10-30 parts of tomatoes, 10-30 parts of blueberries, 5-10 parts of porcine plasma protein powder and 3-5 parts of grape pips. Meanwhile, the invention further discloses a preparation method of the compound. The health-care compound has obvious antioxidant and anti-aging effects, the raw material compatibility is precise and appropriate, the raw materials have synergistic interaction, and the anti-aging health-care compound does not have any side effects, and can be taken for a long term.

The invention discloses a decolorizing, deodorizing and antioxidant yak meat blood sausage. The decolorizing, deodorizing and antioxidant yak meat blood sausage consists of the following components inparts by weight: 100 parts of raw materials, 3.5 parts of edible salt, 0.45 part of five spice powder, 0.4 part of chicken essence and 0.2 part of cooking wine, wherein the weight ratio of yak meat to pig fat is 8: 2, porcine hemoglobin polypeptide accounts for 10-40g / kg of the dosage of the raw materials, and the dosage of the porcine plasma accounts for 10-40g / kg of the dosage of the raw materials. The invention further discloses a preparation method of the decolorizing, deodorizing and antioxidant yak meat blood sausage. According to the yak meat blood sausage obtained according to the technical scheme, dark black and blood smell of traditional blood sausages are removed, the color is yellowish, and the yak meat blood sausage has good color, flavor and chewiness. Besides, the product has a certain antioxidant capacity, can remarkably reduce oxidation of fat and protein, also has a certain antibacterial capacity, and can obviously prolong the shelf life. Theoretical basis and effective ways are provided for deep development and utilization of livestock and poultry blood.

The invention discloses feed for microptenus salmoides wintering and a preparation method thereof. The feed is prepared from steam fish meal, insect protein porcine plasma protein, cassava starch, seaweed gel, yeast hydrolysate, grease, immune complex, mineral complex, vitamin complex and glycine betaine, wherein the immune complex consists of a yeast cell wall and selenium methionine. The feed can meet the nutritional requirement of microptenus salmoides during winters, and can improve the capacity of the microptenus salmoides for resisting nocardia infection and improve the wintering survival rate of the microptenus salmoides.

The invention discloses pomegranate and cherry lung-clearing honey which is prepared from the following raw materials in parts by weight: 700-750 parts of honey, 9-10 parts of nutrition additives, 16-20 parts of grenadine juice, 3-5 parts of pineberry, 3-4 parts of corn kernels, 10-14 parts of fresh prune, 3-5 parts of bread, 10-20 parts of monstera deliciosa fruits, 2-3 parts of porcine plasma protein, 18-20 parts of hericium erinaceus, 10-14 parts of cherries, 10-14 parts of tomatoes, 0.8-1.2 parts of narcissus bulb, 1.5-2.2 parts of scindapsus aureus, 1-2 parts of fourleaf peperomia herb, 1-1.2 parts of Trichosanthes kirilowii Maxim, 1-1.2 parts of bunge cherry seed and 2-3 parts of dried tangerine or orange peel. On the basis of remaining the natural nutritional ingredients of honey, a food with nutrition and health care effects is produced through the scientific formula and advanced manufacturing process. After being eaten frequently, the effects of removing heat from the lung to relieve cough, dispelling wind and eliminating dampness and activating blood circulation to dissipate blood stasis can be achieved, and thus the product is an optimal health food for modern women in pursuit of beauty.

The invention discloses a protein composite edible film for fried food and a preparation method thereof. During preparation of the film, distilled water is used as a solvent, the content of pig plasmaprotein powder is 8% (w / w), the content of ovalbumin powder is 3% (w / w), the addition amount of glycerol is 20% of the total mass of the protein powder and the ovalbumin powder, a uniform and stableliquid is prepared through magnetic stirring and ultrasonic dissolution promotion, and film treatment is carried out before food is oiled. Through testing of the fried meatballs, the protein compositefilm can effectively reduce oil content and water loss, and the defect of high oil content of the fried food can be effectively improved while the crispy outside and tender inside taste of the friedfood is guaranteed. According to the invention, the problem that fried foods are easy to absorb oil is efficiently solved, and the effect of the composite film is better than that of a single-component film.

The invention discloses a method for separating and purifying pig plasma globulin, wherein the method comprises the steps: step 1, preparation of a precipitate rich in globulin: adjusting the proteincontent to 5%; adjusting the pH value and temperature; adjusting the pH after completion of addition of ethanol; carrying out stirring reaction; and swing to wash a centrifugal cup with a 14.5% ethanol balance solution, precooling the centrifugal cup, centrifuging the product, taking a supernatant, and cooling the supernatant to -4.8 to -5.5 DEG C, and filtering for standby application; step 2, separation of the globulin: preparing and separating the globulin; and carrying out press filtration separation of the globulin; and step 3, purification of the globulin. The extraction process is established for the pig plasma globulin, and the obtained product is a single protein; by adjustment and control of the temperature, pH and other parameters, and comprehensive use of the ethanol precipitation and dialysis technology, the target protein has high purity and high extraction rate; the price of the used materials is low, so the cost is greatly reduced; the whole separation and purificationprocess takes less than 10 h, so the separation efficiency is high; the pig plasma globulin is a high value added product and the utilization value of pig blood can be significantly improved.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content of the porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is applicable in screening mass samples.

The invention discloses a feeding bean material capable of improving the meat quality and taste of grass carp. The feeding bean material is prepared from the following raw materials by weight: 80 to 90 parts of soybean, 15 to 17 parts of broad bean, 7 to 8 parts of bamboo leaf, 4 to 5 parts of sweet fermented flour sauce, 3 to 4 parts of cooking wine, 1 to 2 parts of salt, 8 to 12 parts of tea leaf, 20 to 30 parts of leaf of Daphniphyllum macropodum Miq., 5 to 6 parts of lotus wine, 2 to 3 parts of aquilaria wood and a proper amount of porcine plasma protein powder, calcium carbonate and water. The prepared bean material presents the fragrance of bamboo leaf, is capable of clearing heat and lowering fire, has abundant and balanced nutrients, enables grass carp to grow healthily and rapidly and improves the meat quality of grass carp.

The invention relates to a low-fish-meal Micropterus salmoides compound feed as well as a preparation method and application thereof. The compound feed comprises the following components in parts by weight: fish meal, chicken meal, pork meal, spray-dried porcine blood globulin powder, spray-dried porcine plasma protein powder, high temperature and high pressure hydrolyzed feather meal, squid extract, shrimp extract, corn gluten meal, soybean meal, fish oil, soybean phospholipid oil, brewer yeast, a traditional Chinese medicine complexing agent, pea starch, gluten powder, calcium dihydrogen phosphate, taurine, chlorine choline, multivitamins, compound minerals, an antioxidant, a mold inhibitor and rice bran meal; the traditional Chinese medicine complexing agent includes radix scutellariae,cortex phellodendri and rhubarb in parts by weight of (1-3):(2-4):(4-6). The low-fish-meal Micropterus salmoides compound feed can substantially reduce the use amount of fish meal while fully meetingthe nutritional needs of growth of Micropterus salmoides, maintain the growth rate of Micropterus salmoides and the feed utilization rate, and alleviates the consumption of the fish meal in feed industrial production.

The invention discloses a preparation method of solid fat based on pig plasma protein, and belongs to the field of food processing. The method comprises the following steps: (1) adjusting the pH value of a pig plasma protein solution with the concentration of 1.0-3.0% to 6.0-6.5, adding kappa-carrageenan with the final concentration of 0.1-0.7%, and stirring for dissolving; (2) heating the solution obtained in the step (1) at 82-90 DEG C for 10-30 minutes; and (3) cooling the solution obtained in the step (2), adding plant oil with the volume 3-4 times that of the solution, and performing high-speed shearing to prepare solid grease with good stability. The obtained product can be used as non-frying fat to replace animal fat or hydrogenated vegetable oil, the health problem caused by high cholesterol content in the animal fat and trans-fatty acid in the hydrogenated vegetable oil is avoided, and the product is good in stability, has a very good protection effect on functional active ingredients and unsaturated fatty acid, and can be used for preparing non-frying oil. The method has wide application value in the fields of food, medicine and cosmetics.

The invention provides a preparation method of anti-human lymphocyte immunoglobulin, which comprises the steps of: immunizing healthy pigs with human peripheral blood T lymphocytes or human thymocytes, collecting and separating pig plasma, making it with 20% ethanol, and using A low-temperature high-speed centrifuge or a filter press separates the precipitate of component I+II+III, and after making it with 14% ethanol, separates and collects the supernatant, and then makes it with 25% ethanol, separates the precipitate of component II, and then The anti-human T cell porcine immunoglobulin can be obtained through steps such as immunoglobulin purification, configuration and virus inactivation. Compared with the currently used ammonium sulfate salting-out method, the process of the present invention has the advantages of mass production, simple operation and easy mastery, short production cycle, less reagents used, low cost, no environmental pollution, etc., and overcomes the difficulties existing in the existing process. Large-scale production, low output, large waste, environmental pollution, high production cost, complicated production process, various chemical reagents used, long production cycle, high equipment occupancy rate, etc.