175 results about "Porcine blood" patented technology

The invention discloses a preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and a product thereof. The preparation method comprises steps of: (1) carrying out micro-filtering on pig serum protein; (2) inoculating the filtered pig serum protein with bacillus subtilis bacterial suspension and carrying out fermentation; and (3) decentralizing fermentation liquor, collecting liquid supernatant, and carrying out concentrating, freezing and drying to obtain the finished product. The pig serum protein antioxidant peptide prepared by the method has higher antioxidation activity, capability of eliminating free radical and better antioxidation effect both in vitro and in vivo. The preparation method is simple for operation, and is safe and reliable compared with the method of synthesizing antioxidant; and the preparation method is applicable to industrial production and can greatly enhance the utilization rate of pig serum protein resource and raise the comprehensive utilization level of pig blood so as to effectively increase additional value of pig blood resource.

The invention discloses a fluorescent carbon quantum dot having peroxidase catalytic activity and a preparation method of same. The preparation method includes the following steps: 1) grinding animalblood, (such as hog blood, sheep blood, cattle blood, chicken blood and the like) which are purchased form market, in a mortar into paste, adding deionized water to prepare a turbid liquid, performingultrasonic treatment for 20 min, and adding a less amount of the turbid liquid into a high-pressure reaction kettle to perform a hydrothermal reaction; 2) after the reaction finished, cooling a reaction product to room temperature, filtering the reaction product to remove residues through a filter membrane being of 0.22 [mu]m, and centrifuging a filtrate to remove non-soluble precipitates; 3) performing rotary evaporation to a supernatant to increase the concentration of the carbon quantum dot solution, and freeze-drying the solution to obtain blue fluorescent carbon quantum dots which have strong peroxidase catalytic activity. The synthesized carbon quantum dot powder can be stored for a long time and can be blended according to actual concentration demand when being used. The preparation method has simple operation process and low cost. The carbon quantum dot is high in fluorescence yield, can be directly used in the fields of cell and biological imaging, bio-detection, catalysis, etc.

The invention relates to a method for extracting superoxide dismutase (SOD) from porcine blood red cells, in the method, anticoagulation treatment is adopted for preventing blood coagulation and breaking of blood cells. Colloid mill breaking and low-temperature swelling are adopted for releasing matters dissolved in the blood cells to the maximum extent so as to get hemolysate. Thermal change is performed on the hemolysate twice for removing impurities, removing hemoglobin and a large number of impurity proteins so as to get SOD crude enzyme liquid, and ultrafiltration concentration, gradient elution, dialysis, salt removal and freeze-drying are further performed on the SOD crude enzyme liquid so as to get an SOD product. The method is simple to operate and easy to master; furthermore, by adopting the method, the product quality can be effectively improved, the production cost can be reduced, the production cycle can be shortened, the production efficiency can be improved, and the method is applicable to industrialized production.

The invention discloses a cleaning effect monitoring card for cleaning and disinfecting equipment and a preparing method thereof. The cleaning effect monitoring card comprises a novel simulant pollutant and a stainless steel carrier. The novel simulant pollutant is prepared from protein, starch and fat. As protein, starch and a chromogenic substance are connected through chemical bonds, the problem of binding fastness between a chromogenic substance and a stimulant substance on a traditional indicator card is solved fundamentally. Pig blood protein is adopted as the protein component in the simulant pollutant, and human body blood components are highly simulated. The novel stimulant pollutant prepared in the formula is printed on the stainless steel carrier according to a specific pattern to prepare the cleaning effect monitoring card, and the two characteristics of blood are simulated further. Blood after operation is dried up on the surface of a metal instrument, the dried blood cannot be thoroughly cleaned away with water, and therefore the purposes of high simulation and accurate indication of the whole cleaning effect monitoring card are achieved. The cleaning effect monitoring card is easy and convenient to use, and results are easy to judge and read. The cleaning effect monitoring card is free of blood products, safe, nontoxic and suitable for all cleaning processes.

The invention discloses a method for extracting heme. The method comprises the following steps: carrying out centrifugal separation on fresh pig blood and collecting porcine blood corpuscles; after mixing the porcine blood corpuscles with water at a certain ratio, and stirring at a high speed so that membranes of the porcine blood corpuscles rupture; adjusting the temperature to 30-70 DEG C and the pH to 4.0-9.0, adding trypsase for enzymolysis for 6-24 hours; then, adjusting the pH of enzymatic hydrolysate after enzymolysis to 4.0-9.0 and heating to 50-90 DEG C, centrifugally separating the enzymatic hydrolysate and collecting sediment, wherein the sediment is a crude heme product; adding a sodium hydroxide solution to the crude heme product, heating to 50-90 DEG C, and stirring for dissolving the crude heme product; after cooling the solution, adding CMC, stirring evenly, centrifugally separating, and collecting CMC-heme; and separating the CMC with an ultrafiltration membrane and drying the heme, thus obtaining the finished product of heme. According to the method, no organic solvent is needed, thus achieving good safety and environmental friendliness; and production process is simple and controllable, and the purity is over 85%.

The invention relates to the technical field of feed production, particularly to soft-shelled turtle feed with spray-dried pig blood cells added. A formula of the feed comprises components in parts by weight as follows: 0.5-0.8 part of fish meal, 35-42 parts of corn starch, 6-9 parts of the spray-dried pig blood cells, 18-22 parts of soybean meal, 5-8 parts of wheat bran, 4-8 parts of fly maggot powder, 2-3 parts of bone meal, 3-5 parts of shell powder, 2-3 parts of fish oil, 3-5 parts of spirulina powder, 0.2-0.3 part of chlorine calcium carbonate and 0.02-0.03 part of compound vitamins. A preparation method of the spray-dried pig blood cells comprises steps as follows: preparation of a pig blood hemoglobin solution : performing primary ultrasonic treatment; pretreatment: performing physical deoxidation, adjusting the pH value and performing secondary ultrasonic treatment; segmented enzymolysis decoloration; activated carbon decoloration: adding activated carbon accounting for 1-2% of the weight of an enzymolysis decoloration solution for decoloration treatment at the temperature of 25-35 DEG C for 5-10 min; aftertreatment; spray drying. The method is good in decoloration effect, the spray-dried pig blood cells with high protein content and low ash content can be prepared, and the prepared soft-shelled turtle feed is high in safety and high in nutritional value and does not affect the color and the quality of a water body.

The invention discloses a method for preparing an ABP (antibacterial peptide) from porcine hemoglobin. The method comprises steps as follows: porcine blood corpuscles are obtained through separation of porcine whole blood and then subjected to hemolysis, enzymolysis, decoloration, ultrafiltration, vacuum concentration and freeze-drying, and the ABP is obtained; the dosage of trypsin is 3000 U/g, and the enzymolysis reaction time is 4-6 h under conditions that the pH is 8 and the temperature ranges from 45 DEG C to 55 DEG C. The method for preparing the ABP from the porcine hemoglobin is low in cost and good in effect.

The invention provides a method for extracting immunoglobulin G from swine blood, comprising the following steps of: separating out blood plasma liquid from the swine blood subjected to anticoagulant treatment by using a tubular separator; mixing the blood plasma liquid and purified water in a proportion; uniformly stirring; adding a 20-30% saturated ammonium sulfate solution to carry out primary salt precipitation; adjusting a pH value to be 5.50-5.80; standing at constant temperature; centrifuging at high speed; adjusting the concentration of ammonium sulfate to be 30-40% by using supernate and then carrying out secondary salt precipitation; adjusting a pH value to be 7.20-7.60; standing at constant temperature; centrifuging at high speed to obtain an immunoglobulin G precipitate; dissolving the precipitate by using the purified water; and carrying out ultrafiltration and carrying out spray drying at low temperature to obtain an immunoglobulin G finished product. The extracting process is simple to operate, is capable of effectively shortening a production period, reducing the production cost and improving the product quality, and is suitable for industrial production, and the produced immunoglobulin G has the purity of 75% and the recycling rate of 88%..

The present invention discloses a technique for extracting high-purity porcine serum albumin. The technique includes the following steps: sodium citrate and sodium chloride are added into porcine blood to separate plasma and erythrocytes; after being centrifugated, filtered by a membrane and extracted, the plasma is put into an interlayered reactor; alcohol, sodium caprylate and sodium chloride are added into the plasma and stirred slowly after the interlayered reactor is heated; hydrochloric acid is added to adjust the pH value for two times; after centrifugation, supernatant is added into precipitating reagent, ground into paste and stirred; after being refrigerated overnight, the mixed solution is centrifugated and left for precipitation; the precipitate is resolved in water and, after the pH is adjusted by sodium hydroxide solution, kept still; supernatant is separated out by centrifugation; and after membrane filtration and virus inactivation, the filtrate is frozen out into the finished product. The technique has the advantages of little material usage, short process flow, high production efficiency, easy operation, low production cost and high product quality and is suitable for mass production. The product can be used as an auxiliary material for the preparation of fibrin glue sealant, a biochemical material and a substitute for other albumins.

The invention discloses a preparation method of environment-friendly bait for pinna and belongs to the field of aquaculture. The method is characterized in that high quality benthic diatom is cultured by an optimized culture medium, magnetized water, green light, palm bark, a glass goldfish bowl and the like, and then the mixed bait for the pinna is prepared by taking the benthic diatom as a major component and adding a small amount of sheep manure, rabbit manure, porcine blood powder, yeast powder, corn distiller, potamogeton malaianus powder, water caltrop powder, hydrilla varticillata powder, bone tankage, earthworm powder, fly maggot powder, artemia powder, kelp powder, garlic powder, methionine, lysine, potato powder, locust powder, lucerne meal, clam worm powder, trash fish and spiral shell meat meal. The bait is scientific and reasonable in formulation, nutrient-rich and lower in cost, is used for feeding the pinna and does not pollute water, the pinna grows quickly, has a strong constitution and is high in survival rate, and the yield is increased by 160%.

The invention discloses a method for preparing swine blood active peptide by microwave-accelerated enzymolysis, belonging to the field of finish and deep processing and comprehensive utilization of animal byproducts. The method comprises the steps of anticoagulation of swine blood, centrifugal separation, ultrasonic wall breaking, microwave-accelerated enzymolysis, secondary enzymolysis, inactivation, membrane filtration and spray drying. The product can be used as a raw material or additive for various foods. Compared with the traditional methods, the protein molecules move violently due to microwave-accelerated enzymolysis, the enzymolysis velocity can be obviously improved and the enzymolysis time is shortened. The method has simple process, low cost and environment friendliness and is suitable for industrial production of deep processing of swine blood.

The present invention relates to a pig blood treatment method, particularly to a pig blood extracting and using method, which comprises: preparing a pig blood hemoglobin solution, wherein an additive is added to pig blood, centrifuging separation is performed to obtain the first supernatant layer and the first precipitation layer, separating is performed to obtain the first precipitate, and a first ultrasonic wave treatment is performed; pre-treating, wherein physical deoxidation is performed, the pH value is adjusted, and a second ultrasonic wave treatment is performed; carrying out sectional enzymolysis decolorization; carrying out active carbon decoloration, wherein active carbon with the weight of 1-2% of the mass of the solution is added, and a decoloration treatment is performed for 5-10 min at a temperature of 25-35 DEG C; post-treating, wherein the pH value is adjusted, and centrifuging separation is performed to obtain the second supernatant layer and the second precipitation layer; taking the second supernatant layer, and carrying out ultra-filtration concentration separation to obtain the second concentrated solution; preparing pig blood plasma protein powder, wherein the first supernatant layer is subjected to spray-drying; preparing pig hemoglobin powder, wherein the second concentrated solution is subjected to spray-drying; and preparing a heme peptide, wherein the second precipitation layer substance is taken and is subjected to roller drying. The method of the present invention has the good decolorization effect, and the product with the high protein content and the low ash content can be prepared.

The invention belongs to the field of food processes and discloses a method for preparing pig blood nitrosohemoglobin. The method for preparing the pig blood nitrosohemoglobin comprises the following steps: (1) preparing pig blood and preparing hemoglobin solution; and (2) adding 0.2M NaOH solution into the hemoglobin solution, adding sodium nitrite and ascorbic acid at the same time, stirring, heating to 83 to 88 DEG C, reacting for 10 to 18 minutes, cooling to 23 to 28 DEG C after reaction, centrifuging for 1 to 5 minutes at a speed of 2,500 to 32,000r/min, removing supernate, washing a precipitate with solution of ascorbic acid, centrifuging again, repeatedly washing, and obtaining the precipitate which is the nitrosohemoglobin, wherein the molar ratio of pig blood nitrosohemoglobin to sodium nitrite to ascorbic acid is 1:(2-3):(10-13). In the invention, a new method for preparing pig blood nitrosohemoglobin is provided, the byproduct of animal blood is utilized effectively, environmental pollution is relieved, waste materials are changed into valuable materials, and economic benefit is improved.

The invention discloses a cream-flavored leech culture feed and a preparation method thereof. The cream-flavored leech culture feed comprises the following raw materials in parts by weight: 150-170 parts of shrimp bran, 10-20 parts of Pu'er tea-leaf, 40-60 parts of fresh porcine blood, 70-80 parts of soybean meal, 30-40 parts of porcine bone marrow, 2-3 parts of yoghourt, 20-30 parts of rice washing water, 0.4-0.6 part of molasses, 0.2-0.3 part of a yeast extract, 0.1-0.2 part of EM zymocyte, 0.3-0.5 part of dairy salt, 3-5 parts of watery cream, 0.1-0.2 part of sucrose fatty acid ester and 0.4-0.6 part of glucose syrup. The end product is in the state of micro-powder, can be easily dissolved or suspended in water, can be adsorbed on sponge for feeding, is easy for ingestion and digestion in comparison with common large-particle-diameter feeds, and is easy to store and long in guarantee period.

The invention discloses a blood anticoagulant which consists of the following raw materials in parts by weight: 2.5-3 parts of sodium citrate, 0.3-0.4 part of citric acid, 2.2-2.6 parts of glucose, 0.2-0.25 part of sodium dihydrogen phosphate, 1-1.5 parts of ethylenediamine tetraacetic acid disodium salt and 100 parts of water, wherein the raw materials are sequentially added into 100 parts of water, dissolved and uniformly stirred at normal temperature, and 14ml of the blood anticoagulant is dissolved in 100ml of pig blood. The anticoagulation effect of the blood anticoagulant disclosed by the invention is not less than 3 days, and then the phenomenon of blood hemolysis is solved. By adopting the blood anticoagulant, the fibrinogen aggregation phenomenon of the blood is solved.

The invention discloses soluble plasma protein powder and a preparation method thereof. The soluble plasma protein powder is a mixture prepared by uniformly mixing milk powder and plasma protein powder, wherein the milk powder accounts for 10-40% of the total mass of the soluble plasma protein, pig blood accounts for 40-84% of the total mass of the plasma protein powder, and sugar accounts for 6-20% of the total mass of the plasma protein powder; the plasma protein powder is rich in fragrance, free of blood fishy smell and good in solubility and brewing stability. The preparation method comprises the preparation and operation steps of vacuum blood collection, blood anticoagulation, step-by-step enzymolysis deodorization, membrane concentration, addition of ingredients, high-pressure homogenization, spray drying, and baking and aroma extraction. The soluble plasma protein powder is fragrant, sweet and mellow in taste, has strong milk fragrance and uniform particles, and is easy to dissolve and free of caking and bad fishy smell after being brewed. The whole set of technology is adopted to process the pig blood into the convenient and instant plasma protein powder, so that the development and utilization of blood resources are realized, the utilization value of by-products such as the pig blood is greatly improved, and the market vacancy of the food plasma protein in China is supplemented.

The invention relates to a pig blood reuse processing device, and particularly relates to a pig blood globulin powder preparation device including an air filter connected with a dryer; the dryer is connected with a spraying device; an air heater is connected between the air filter and the dryer; the spraying device comprises a granulation fluidized bed and knives, and both sides of each knife are symmetrically provided with two-fluid atomization spray guns; material liquid inlets of the two-fluid atomization spray guns are connected with a disc type separator comprising a disc type separator rotary drum, an inlet and out device, a deslagging part and a wastewater outlet, and the dryer is connected with a dust collector; a shunt sieving device is arranged between the dryer and the dust collector; the shunt sieving device comprises a shunt tube and rotary vibration sieves; the top parts of the rotary vibration sieves are communicated with the dust collector; the dust collector comprises a cyclone separating device and dust removal devices; an outlet of the cyclone separating device is connected with a heat exchanger. The pig blood globulin powder preparation device has the advantages of uniform granulation, good separation effect, good recovery performance, and full recovery of waste heat.

The invention discloses a fermenting method for a porcine haemoglobin feed. The fermenting method comprises the following steps: (1) uniformly mixing bran mixture, fresh porcine blood and dried blood in a ratio of 1:1:0.15 or 1:1:0.15; (2) uniformly mixing a yellow mould family strain No.6, a yellow mould family strain No.7 and a lactobacillus acidophilus PB1 in a ratio of 3:3:4, thus obtaining a leavening agent; (3) adding the leavening agent obtained in the step (2) accounting for 0.1-0.2% of the total material weight, uniformly mixing with the material obtained in the step (1), then adding water, and uniformly stirring; and (4) putting the material obtained in the step (3) into a container, then exhausting the air, covering with a plastic film and carrying out sealed fermentation, and then carrying out spray drying, thus obtaining the porcine haemoglobin feed. According to the fermenting method disclosed by the invention, the soluble substances can be increased by 45.2% and the digestibility can be improved by 25-30%, protein absorption and utilization rate is high, palatability is good, and the developed porcine haemoglobin feed is detected to contain no aflatoxin B1 and is safe and reliable; meanwhile, the porcine haemoglobin feed has the advantages of rich raw material sources, low price, simple production method and long storage life and has very broad market prospect.

The invention relates to an industrial production method of low peptides and amino acids through enzymolysis of pig whole blood by taking proteins of pig whole blood as raw materials. The industrial production method comprises the links of clean blood sampling, whole blood enzymolysis, evaporation and concentration, spray drying, product packaging and the like. A glassed steel reaction kettle and a glassed steel evaporating pot are creatively selected as core equipment in the method. A steam-warm water circulating system is invented, and the industrial production for high temperature sterilization of pig blood as well as low temperature enzymolysis and high temperature enzyme deactivation in the same equipment is realized. A three-step production process is shortened into one-step production, and the number of the equipment is reduced from three to one. Therefore, the quality of products is improved, the digestibility is up to over 96%, the production period is greatly shortened, and the cost is reduced. A production technology is easy to master, the environment is not polluted in the production process, the quality of the products conforms to the national food safety standard, and the products can be widely used as food nutritive fortifiers and high-grade feed additives.