The invention discloses a compound premix for laying hens and a preparation method and a using method thereof. The content of main nutrition in every kilo of the compound premix is 170,000IU of vitamin A, 350,000IU of vitamin D, 200IU of vitamin E, 30mg of vitamin K3 (calculating according to menaquinone), 140mg of vitamin B1, 110mg of vitamin B2, 680mg of vitamin B, 120.2mg of vitamin B, 15mg of folic acid, 3.6mg of biotin, 700mg of niacin, 250mg of pantothemic acid, 1,800mg of ferrum, 160mg of copper, 1,400mag of zinc, 1,300mg of manganese, 10mg of iodine, 7mg of selenium, 18,000mg of methionine, 6,000mg of choline chloride, 4,000mg of phytase and 100,000mg of bacillus subtilis. The compound premix can obviously improve laying rate of laying hens of 35 weeks, reduces the ratio between the feed and eggs and improves quality of eggs.