Biological feed additive capable of raising lean meat frequency of animals, its preparation method and application
A technology of biological feed and lean meat rate, which is applied in the direction of biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of increased number of underweight, increased chance of disease occurrence, and poor population uniformity, etc., to achieve Improves body condition, reduces animal immune response, highly targeted effects
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[0079] 1. Preparation of β-mannanase
[0080] (1) Fermentation culture
[0081] a. Bacteria and culture medium
[0082] Strain Bacillus Lentus GL-5 seed medium (mass concentration, g / L): locust bean gum 15, beef peptone 8, yeast extract 4, KH 2 PO 4 and MgSO 4 ·7H 2 O 0.25; fermentation medium (mass concentration, g / L): locust bean gum 15, beef peptone 8, yeast extract 4, KH 2 PO 4 and MgSO 4 ·7H 2 O 0.25.
[0083] b. Bacteria culture
[0084] Pick a single colony of Bacillus Lentus GL-5 and inoculate it in the seed medium, culture at 35°C, shaker speed 200r / min, time 24h, the viable count is greater than 10 10 a / ml;
[0085] c. Fermentation culture
[0086] According to the inoculation amount of 5% to 10%, the shake flask culture strains were inserted into the fermentation medium, the culture time was 48 hours, the temperature was 37°C, the ventilation rate was 1L / L / min, and the enzyme activity of the fermentation broth was measured in about 48 hours. The enzyme a...
Embodiment 1
[0091] 1. Weigh 100 g of freshly prepared β-mannanase;
[0092] 2. Take by weighing lysine (feed grade) 20g, soybean oil (feed grade) 20g, 33% ethoxyquinoline powder (feed grade) 0.5g, corn gluten powder (feed grade) 859.5g, add respectively successively β-mannanase, fully stirred and mixed to obtain a feed additive product of the present invention.
Embodiment 2
[0094] 1. Weigh 120 g of freshly prepared β-mannanase;
[0095] 2. Take by weighing lysine (feed grade) 25g, soybean oil (feed grade) 15g, 33% ethoxyquinoline powder (feed grade) 0.6g, corn gluten powder (feed grade) 839.4g, add respectively successively β-mannanase, fully stirred and mixed to obtain a feed additive product of the present invention.
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