This invention discloses broad-spectrum antibacterial peptides WP-4 and WP-6, their compositions, and applications, belonging to the fields of
antimicrobial peptide technology and
biomedicine. This invention obtains a series of
antimicrobial peptides by truncating and rationally modifying template peptides to balance the relationship between
charge number, hydrophobicity, and amphiphilicity. By measuring
antibacterial activity, stability, hemolytic activity, and
cytotoxicity, two novel
antimicrobial peptides, WP-4 and WP-6, with
high activity and
low toxicity were obtained, and their
amino acid sequences are shown in SEQ ID NO. 5 and SEQ ID NO. 7. The novel
antimicrobial peptides obtained by this invention possess broad-spectrum
antibacterial activity, good stability under temperature, pH, salt
ion,
pepsin, and serum conditions, and exhibit no significant hemolytic activity and low
cytotoxicity. They are characterized by rapid bactericidal action and low likelihood of inducing
drug resistance. Furthermore, the
antimicrobial peptides of this invention can enter cells and have a strong
inhibitory effect on
intracellular bacteria, showing broad application prospects.