The invention provides a method for measuring internal force of a guy cable in active service, which comprises the following steps: selecting two points on a guy cable to be measured, and fixing the two points so that a cable section to be measured between the two points is formed into a string pattern; selecting a point on the cable section to be measured between the two points, jacking a height at the point, and measuring the height and the jacking force required for jacking the height; and according to the height, the jacking force and the distance between the two points, using the stress rigidization effect of the guy cable and a principle of statical equilibrium to evaluate the internal force of cable section to be measured. The method has the advantages of convenient measurement, high precision and low measuring cost. The invention also provides a measuring device using the method.