196 results about "Particle sorting" patented technology

The invention provides a flow system and method for reliable multiparameter data acquisition and particle sorting. In accordance with the invention, a flow system assesses changes in the pattern of data collected in successive time intervals and actuates one or more corrective actions whenever the changes exceed predetermined limits. The present invention overcomes problems associated with collecting data and sorting and enumerating particles in flow cytometry systems that operate for prolonged periods or that must accommodate samples that vary widely in quality.

An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.

A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.

An automated monitoring and alignment system to position the mechanical and optical components of a flow cytometer to enhance the consistency, performance, and efficiency of particle sorting and analysis applications.

An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.

The invention relates to a sorter and a sorting method for separating cells and particles. The sorter comprises a Raman spectrum acquisition analysis system, a micro-flow control system and a cell / particle sorting system, wherein the micro-flow control system is connected with the Raman spectrum acquisition analysis system; the Raman spectrum acquisition analysis system is connected with the cell / particle sorting system; and the cell / particle sorting system is connected with the micro-flow control system. The invention also provides a sorting method for separating the cells and the particles.The sorter can effectively identify and sort nanometer-micron sized cells, nano materials, particles and cell contents, and because the Raman spectrum has the characteristics of high speed, non-invasive property, high information content and the like, the sorting method provides richer characteristic information of the cells or the particles in the same time period compared with methods of fluorescence or radio isotope labeling and the like.

A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.

Most automatic particle classification methods produce errors. The invention provides a method for improving the accuracy of particle classification while shortening the amount of manual review time required from the operator. The method uses class weights, which are statistically-derived correction factors that accounts for frequency of classification errors. A first class weight and a second class weight are assigned to the first class and the second class, respectively. The number of particles in each of the first and the second classes is multiplied by the first class weight and the second class weight, respectively, to generate a corrected number of particles in each of the classes. If particles are reclassified, the class weights are recalculated in response to the reclassification. The method is usable with a complete classification where all the particles in a sample are classified, or a selective classification of a subset of the particles in the sample.

The present invention provides a fine-particle sorting technique that uses optical pressure (optical force) and that can be applied in place of heretofore known cell sorting techniques; and a fine-particle recovering technique therefor. The fine-particle recovering method of the present invention comprises directing a laser beam toward a flow path of fine particles in such a manner that the laser beam crosses the flow direction of the fine particles to thereby deflect the direction of movement of the fine particles to be recovered, in the direction of convergence of the laser beam. The sorting method of the present invention comprises performing flow cytometric sorting of fine particles according to the above recovering method of the present invention.