37 results about "Invertebrate" patented technology

The invention relates to the technical field of pesticides, and discloses an isoxazoline compound, a preparation method and application thereof, an agricultural composition and a method for preventing and treating invertebrate pests. The invention provides an isoxazoline compound with a structure as shown in a formula (I), or an agriculturally acceptable salt thereof, or a stereoisomer thereof. The isoxazoline compound and the agriculturally acceptable salt thereof provided by the invention show very high prevention and control effects on various pests, especially spider mites represented by tetranychus cinnabarinus, tetranychus urticae, tetranychus shennieri, panonychus citri and the like, and lepidoptera insects represented by plutella xylostella and the like; the acaricidal composition can be used for preventing and treating various pests and harmful mites, has efficient acaricidal and insecticidal activity, and has a good application prospect. # imgabs0 #

The disclosure provides compositions and methods for treating and vaccinating invertebrates and invertebrate populations from diseases.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a cross-animal general skeleton-derived hematopoietic stem cell marker gene set and a screening method thereof. According to the invention, a bone-derived hematopoietic stem cell marker gene set universal across animal categories is constructed for the first time, and the bone-derived hematopoietic stem cell marker gene set comprises at least five of Cdc42, Cbx, Tfam, Denr, Mcts1, Ak2, Ruvbl, Ahcy, Nna and Vdac; the hematopoietic stem cell marker gene set is obtained through cross-species homologous gene screening, the defect that a traditional vertebrate marker has no orthohomology in invertebrates is overcome, accurate recognition of HSC in shells is achieved, the hematopoietic stem cell marker gene set has species universality and cell specificity, the immune state of aquatic animals can be evaluated, disease-resistant breeding can be guided, and the application prospect is wide. And molecular evidence is provided for analyzing an evolution path of a hematopoietic system from invertebrates to vertebrates.

To provide a low-cost land-based aquaculture system for marine invertebrates that does not depend on natural seaweeds.SOLUTION: This land-based aquaculture system comprises: a feed tank for culturing seaweeds serving as feed for marine invertebrates by using seawater; a rearing tank for rearing the marine invertebrates by using seawater; and a gas supply device for supplying a gas containing carbon dioxide at a higher concentration than that in the atmosphere to the seawater in the feed tank.SELECTED DRAWING: Figure 1

The application discloses a kind of micro invertebrate automatic detection method, comprising the following steps: step S1: obtaining water sample;Step S2: sample concentration;Step S3: optical detection;Step S4: filter detection unit cleaning;Step S5: detect filter detection unit filter membrane pollution;Step S6: replace filter detection unit;Step S7: manually replace filter detection unit;The application is concentrated sample for detecting chironomid larvae by realizing automatic filtration collection to water sample in step S2, detects chironomid larvae in concentrated sample in filter detection unit by sample imaging unit in step S3, and filter detection unit is cleaned by step S4;Filter detection unit with filter membrane pollution is replaced by step S5 and step S6;The application does not need manual collection and detection chironomid larvae, reduces the cumbersome step of manual operation, and is simple to operate, step is simple, improves the frequency and efficiency of sample collection, sample detection.

An apparatus and system for generating digital images of specimens and identifying the species of an invertebrate, such as a mosquito, are disclosed. The apparatus includes an imaging device capable of capturing images, light sources to illuminate the optical field of view of the imaging device, a housing enclosing the imaging device and the light source, and an opening in the housing where a specimen tray can be inserted. The specimen tray can include a lid and multiple wells separated by transparent walls, each well capable of holding a specimen. The wells are fully enclosed when the lid is placed on the tray and the tray includes markings on both sides indicating the tray orientation, as the tray is transparent on the lid and base of the tray to enable imaging from two sides of the tray. The housing and tray also includes detents and indents for locking the tray into the correct placement when inserted into the slot. The imaging device is capable of capturing high quality images of two distinct viewpoints of all specimens in the wells.

To provide a feed for marine invertebrates such as abalones and urchins, to provide a method for producing the feed for marine invertebrates, to provide a method for farming marine invertebrates, to provide a method for accelerating the length of shells, and to provide a method for increasing the content of fatty acids.SOLUTION: Provided are: a feed for marine invertebrates, characterized by containing microalgae belonging to the genus Euglena; a method for producing the feed for marine invertebrates, characterized by performing a solidification step of solidifying microalgae belonging to the genus Euglena; a method for farming marine invertebrates and a method for accelerating the length of shells, which comprise feeding microalgae belonging to the genus Euglena to marine invertebrates; and a method for increasing the content of fatty acids, which comprises feeding microalgae belonging to the genus Euglena to increase the content of fatty acids contained in edible parts of marine invertebrates.SELECTED DRAWING: Figure 5

The utility model relates to the technical field of invertebrate breeding, and discloses an earthworm collecting device for earthworm breeding, which comprises an earthworm breeding box and pulleys arranged on the bottom surface of the earthworm breeding box, vent holes are arranged on two sides of the earthworm breeding box, a breeding collecting device is arranged in the earthworm breeding box, and the breeding collecting device is arranged in the earthworm breeding box. A placement plate is arranged above the breeding collection device, and a breeding induction device is arranged on the top surface of the placement plate. By arranging the two symmetrical earthworm breeding boxes and arranging the telescopic bases and the electric telescopic rods at the bottoms of the breeding boxes, when earthworms are collected, the two breeding boxes only need to be pulled open towards the two sides, and then a color temperature adjusting and controlling lamp and a water spraying plate on one side are controlled, so that the earthworms are attracted by the breeding box on the other side, move to an earthworm outlet and fall into a collecting box; the earthworms can be collected without arranging a suction box, the space in the device is saved, the breeding efficiency is improved, and the device is simple in structure, convenient to operate and high in practicability.

The invention discloses a benthic invertebrate screening device based on ultrasonic waves. The benthic invertebrate screening device comprises a pre-screening bin; an ultrasonic assembly I; a plurality of high-definition cameras I; a display screen I; a controller I; a main screening bin; an ultrasonic assembly II; a plurality of high-definition cameras II; a controller II; the flushing assembly comprises at least two nozzles which are detachably arranged at the top of the main screening bin; and a pump. The invention further provides a screening method applying the benthic invertebrate screening device based on the ultrasonic waves, according to the screening method, the marine benthic invertebrates in the samples can be accurately and efficiently screened, damage to the marine benthic invertebrates caused by manual sorting is effectively avoided, and the screening efficiency is improved.

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.

The utility model discloses a high-flux small aquatic animal metabolic rate measuring device which comprises a test cylinder, a placing table, a breathing chamber and a breathing cup, a temperature controller is connected in the test cylinder, a main body pipeline is connected to the upper side of the placing table, a water pump is fixedly connected in the test cylinder, and a water conveying pipe is fixedly connected between the water pump and the main body pipeline; covers are arranged on the front side and the rear side of the breathing chamber, a round hole is formed in the exterior of the main body pipeline, a single-pass small pipe is fixedly connected to the exterior of the round hole, a flow stabilizing mechanism is installed in the breathing chamber, a water outlet pipe is fixedly connected to the exterior of the breathing chamber, a regulating valve is fixedly connected to the exterior of the water outlet pipe, and an oxygen dissolving instrument is installed in the breathing cup; the device can be used for measuring the metabolic rate of various aquatic invertebrates, tadpoles, fishes and other small aquatic animals, can realize high-flux measurement of a plurality of samples at the same time, has portability, is easy to assemble and carry, and can ensure the experiment precision by adjusting the water flow speeds of the pipelines to be relatively consistent.

The present invention provides an attachment base for sessile aquatic invertebrates that improves the structure of conventional coral attachment plugs and facilitates the promotion of growth and breeding management of sessile aquatic invertebrates in general. [Solution] The attachment substrate 10 for sessile aquatic invertebrates serves as a base for attaching sessile aquatic invertebrates, and includes a frame 1, an alkaline mineral plate 2 which is a member containing alkaline minerals and is placed on and near the attachment surface to which the sessile aquatic invertebrates attach, an internal magnet 3 which is a first magnetic piece and is placed inside the alkaline mineral plate 2, and an external magnet 4 which is a fixing member. The attachment substrate 10 for sessile aquatic invertebrates is fixed onto an in-aquarium shelf 7 which is a non-magnetic object placed between the frame 1 and the external magnet 4 via the attractive force between the internal magnet 3 and the external magnet 4.

The utility model discloses a marine invertebrate polysaccharide extraction device which comprises an extraction tank, a sealing cover is arranged at the top end of the extraction tank, and the top of the sealing cover is communicated with a feeding pipe. The marine invertebrate tissue can be subjected to efficient centrifugal separation, polysaccharide components can be fully released and separated, the extraction efficiency is improved, the extracted polysaccharide components can uniformly flow out through centrifugal holes, and a water bath heat preservation assembly accurately controls the temperature in the extraction tank through a circulating system of a heating water tank, a water pump, a heating coil and a backflow pipe. It is ensured that the polysaccharide extraction process is carried out under the optimal temperature condition, the stable temperature environment is beneficial to polysaccharide extraction and activity maintenance, the negative effects of temperature fluctuation on the extraction efficiency and the polysaccharide structure are avoided, the heating coils are evenly distributed in the heat preservation cavity, and the temperature stability and uniformity of the extraction process are ensured.

Low doses of fluralaner or another isoxazoline derivative are administered to an animal such as a pet, typically a dog or a cat, preferentially by administration of a repeated dose, at regular intervals or at varying intervals, each dose being of the same or different amount, which leads to efficacy against arthropods, especially fleas and ticks. Various regimens, galenic forms, posologies, therapeutic indications, combinations with other active or synergistic ingredients, and smart devices or methods for release of doses to the animal can be provided, alone or in combinations.

The invention discloses a device and method for invertebrate behavior ecology research, and relates to the technical field of animal behavior research, the device comprises a starting pipe, a heat insulation assembly and an environmental factor regulation and control assembly, and the heat insulation assembly is internally provided with a plurality of channels communicated with an inlet and an outlet; the inlet is connected with the starting pipe; the environmental factor regulation and control assembly comprises a temperature control assembly and at least two branch pipe groups, the branch pipe groups are in one-to-one correspondence with the outlets, and each branch pipe group is connected and communicated with each outlet; the temperature control assembly can adjust the temperature of each branch pipe group; and an environment stimulation source is placed in the branch pipe group. Single-factor environmental stimulation or multi-factor environmental stimulation is applied to the branch pipe groups, so that the environmental stimulation presented in different branch pipe groups is different; enabling the tested animal in the starting tube to autonomously move in the invertebrate behavior ecology research device; and obtaining selection behavior characteristics and data of the tested animal. According to the method, more systematic and comprehensive behavioral data can be obtained, and the experiment accuracy is improved.

The present invention discloses a field survey device and method for assessing the status of invertebrate habitats. The device comprises a first float, a bracket mounted on the first float, a winding device mounted on the bracket, a pull rope mounted on the winding device, and an in-water survey assembly mounted on one end of the pull rope. This technical solution places the float in the waters of the invertebrate habitat to be surveyed, allowing the in-water survey assembly to enter the waters. A wireless module transmits water quality data of the invertebrate habitat collected by a water quality detection sensor and image data of the invertebrate habitat collected by an image acquisition device to an external monitoring terminal for processing, allowing users to assess the status of the invertebrate habitat. This method can replace manual real-time collection of habitat status, reducing the difficulty of the survey and the intensity of manual labor.

The present invention relates to methods of isolating and / or culturing a population of mesangiogenic progenitor cells (MPCs) or cells of the endothelial lineage, wherein the methods comprise the use of marine invertebrate collagen. The invention also relates to compositions comprising MPCs and marine invertebrate collagen and medical uses of such compositions, and medical devices and kits comprising the same.

The present invention belongs to the technical field of benthic animal monitoring, and in particular to a method for monitoring large benthic invertebrates in different types of wadable rivers. The method provides a method for monitoring large benthic invertebrates in wadable rivers through five main steps, namely, classifying rivers by type, laying out monitoring points, selecting sampling areas, sampling, and counting the types and numbers of large benthic invertebrates in samples. The method can effectively and practically monitor large benthic invertebrates in different types of wadable rivers, and provide a reference and reference for carrying out benthic animal monitoring in different types of rivers.

The invention discloses multi-stage selection equipment for aquatic organisms, and relates to the field of aquatic organism selection, the multi-stage selection equipment comprises a selection box and a partition plate arranged in the selection box, the partition plate is provided with a plurality of bottom mud uniform laying devices, and one side of each bottom mud uniform laying device is provided with a plurality of secondary dilution uniform laying devices; and a plurality of suction selecting devices are arranged at the top of the inner wall of the selecting box. According to the selection equipment, efficient, accurate and automatic screening and collection of the benthic large invertebrates are achieved through collaborative design of modules such as bottom mud uniform laying, secondary dilution, suction selection and automatic discharging.

The utility model relates to the technical field of water ecological restoration, and provides an adjustable biofilm culturing device for improving nitrogen and phosphorus retention potential of a small and micro water body. The suspended ball filler on the biological filler filter frame and the MBBR filler in the suspended ball filler can provide good attachment conditions and habitat for periphytic algae, bacteria, fungi, invertebrates and the like, a periphytic biocenosis structure with rich biodiversity is constructed, and the biofilm biomass of the periphytic algae, bacteria, fungi, invertebrates and the like is improved by improving the biofilm biomass of the periphytic algae, bacteria, fungi, invertebrates and the like. The nitrogen and phosphorus retention capacity of the small and micro water body is improved; the height of the device in water can be adjusted according to the depth of a water body through the design of multiple embedded baffles of the fixed plate; according to the utility model, each group of suspended ball fillers can be freely detached, so that damaged suspended balls can be replaced or aged biological membranes can be updated; the material used by the utility model has the advantages of simplicity in manufacturing, low operation cost, large surface area, easiness in biofilm formation, easiness in cleaning, no harm to the environment and the like.

The invention discloses an artificial domestication method for odontobutis suchneri. The method comprises the steps of fishing, temporary rearing in a net cage, running water transportation, indoor temporary rearing and quarantine treatment and culture in a culture pond. According to the method, the dissolved oxygen condition, the pH water temperature, the photoperiod and other environmental regulation and control means in the domestication process are controlled, a three-stage medicated bath scheme of citric acid, glutaraldehyde benzalkonium bromide and oxytetracycline is adopted, and praziquantel is adopted for targeted parasite expelling, that is, environmental stimulation is effectively reduced through environmental regulation and control and use of animal protection products, loss caused by stress and fight is reduced, and the economic benefit is increased. Meanwhile, physical damage caused in the fishing and transporting processes can be effectively treated. In addition, in consideration of the fact that food of the humping head fishes mainly comprises small invertebrates such as shells, snails, crabs and shrimps, bait conversion is completed through specially-made artificial feed and a domestication material management technology in the artificial domestication process. Therefore, the artificial domestication of the wild odontella schuchnoi is realized.

The invention provides a method for biologically preventing and controlling filamentous algae pollution in a water body, which comprises the following steps of: putting filter-feeding invertebrates into the water body to be prevented and controlled to reduce spores of filamentous algae and biomass in an early growth stage; algae-eating animals are put into the water body to be prevented and controlled and used for ingestion of the filamentous algae and inhibition of formation of algae caps of the filamentous algae; the floating type ecological carrier is put into the water body to be prevented and controlled and used for inducing the filamentous algae to form algae covers on the floating type ecological carrier. The filter-feeding invertebrates, the algae-eating animals and the floating ecological carriers are comprehensively used for preventing and treating filamentous algae pollution in the water body, the filter-feeding invertebrates, the algae-eating animals and the floating ecological carriers have high ecological synergy, growth of filamentous algae in the water body can be controlled and the water quality can be improved under the condition of not depending on chemical agents through interaction of multiple organisms, and the pollution of the filamentous algae in the water body can be reduced. And the method has good environmental protection benefits and sustainability.

The utility model relates to a demersal invertebrate flotation device which comprises a flotation frame, an installation frame and a supporting frame, a first screen plate is arranged in a flotation cavity of the flotation frame, the flotation frame is sleeved with the first screen plate in cooperation with the installation frame, and an intercepting screen plate on the installation frame is aligned with the first screen plate. According to the screening device for the benthic invertebrates, due to the fact that the blocking net plate is arranged in the screening device, and the hole diameter of the blocking net holes is larger than that of the first screening net holes, when the benthic invertebrates are sorted, gravels and aquatic plant residues large in size can be blocked through the blocking net plate firstly, then further screening is conducted through the first screening net plate, non-target substances mixed in the benthic invertebrates can be effectively reduced, and the screening efficiency is improved. According to the benthic invertebrate collecting device, the possibility that benthic invertebrates are attached and hidden is reduced, the sorting difficulty is reduced, the sorting efficiency of the benthic invertebrates is improved, and therefore the collecting efficiency of benthic invertebrate samples is improved.

The utility model relates to the field of insect pest investigation and prevention, in particular to an invertebrate early warning or long-term monitoring device. And a support device. The container is a hollow circular truncated cone, the area of the bottom face of the hollow circular truncated cone is smaller than that of the top face of the hollow circular truncated cone, the top face is open, a wide edge wider than the wall thickness of the container is arranged on the edge of the top face, and a plurality of through holes are formed in the side wall of the container. The supporting device is a hollow cylinder with an opening, and two through holes which are in axial symmetry are formed in the side wall of the supporting device. A suspension device is arranged on the two through holes, and the early warning or long-term monitoring device can be suspended on a tree branch according to the requirements of a user so as to trap winged insects; in the using process, corresponding trapping liquid is added into the container according to the requirement of a user, the device is collected according to a sampling plan, and a new round of trapping can be started only by replacing a new container.

The invention relates to the technical field of biological enzymes, and discloses an N-acyl homoserine lactone degrading enzyme as well as a preparation method and application thereof. Wherein the amino acid sequence of the N-acyl homoserine lactone degrading enzyme is as shown in SEQ ID NO. 1. The N-acyl homoserine lactone degrading enzyme disclosed by the invention is derived from a co-epiphytic bacterium Pseudomonas onchidi XY-99 of a marine invertebrate organism, i.e., calf stone, has wide signal molecule degradation activity, can block a quorum sensing pathway of pathogenic bacteria and inhibit the generation of virulence factors and the formation of a biofilm, and can be used for preparing a product for preventing and controlling pseudomonas aeruginosa.

The present invention discloses an environmental monitoring system for water body ecological restoration, which relates to the technical field of water body ecological restoration. During the process of obtaining the water quality indexes of each sampling point of the water body to be restored, through laboratory analysis and measurement, the chlorophyll a concentration and benthic invertebrate information of each sample can be accurately obtained. During the process of monitoring the biological species information of the water body to be restored, by classifying the samples through a microscope and sorting equipment, the individual number of benthic invertebrates and the number of EPT individuals can be accurately obtained. By comparing the biodiversity assessment coefficient with the set interval, the complexity and stability of the ecosystem can be visually evaluated, providing a basis for taking corresponding biodiversity governance measures. During the process of obtaining hydrological change information, through the analysis of the hydrological change assessment coefficient, the flow condition and hydrodynamic characteristics of the water body can be evaluated, providing key data support for the restoration of the ecosystem.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

The application provides a method for controlling filamentous algae pollution in water bodies based on biological control, which comprises the following steps: putting filter-feeding invertebrates into the water body to be controlled, so as to reduce the spores and biomass of early growth stages of filamentous algae; putting algivorous animals into the water body to be controlled, so as to feed on filamentous algae and inhibit the formation of algal mats of filamentous algae; and putting floating ecological carriers into the water body to be controlled, so as to induce filamentous algae to form algal mats on the floating ecological carriers. The application controls filamentous algae pollution in water bodies by comprehensively using filter-feeding invertebrates, algivorous animals and floating ecological carriers, the three have strong ecological synergy, can control the growth of filamentous algae in water bodies and improve water quality without relying on chemical agents through the interaction between multiple organisms, and has good environmental protection benefits and sustainability.