26 results about "Invertebrate" patented technology

The invention belongs to the technical field of biomolecular markers, and particularly relates to a cross-animal general skeleton-derived hematopoietic stem cell marker gene set and a screening method thereof. According to the invention, a bone-derived hematopoietic stem cell marker gene set universal across animal categories is constructed for the first time, and the bone-derived hematopoietic stem cell marker gene set comprises at least five of Cdc42, Cbx, Tfam, Denr, Mcts1, Ak2, Ruvbl, Ahcy, Nna and Vdac; the hematopoietic stem cell marker gene set is obtained through cross-species homologous gene screening, the defect that a traditional vertebrate marker has no orthohomology in invertebrates is overcome, accurate recognition of HSC in shells is achieved, the hematopoietic stem cell marker gene set has species universality and cell specificity, the immune state of aquatic animals can be evaluated, disease-resistant breeding can be guided, and the application prospect is wide. And molecular evidence is provided for analyzing an evolution path of a hematopoietic system from invertebrates to vertebrates.

To provide a low-cost land-based aquaculture system for marine invertebrates that does not depend on natural seaweeds.SOLUTION: This land-based aquaculture system comprises: a feed tank for culturing seaweeds serving as feed for marine invertebrates by using seawater; a rearing tank for rearing the marine invertebrates by using seawater; and a gas supply device for supplying a gas containing carbon dioxide at a higher concentration than that in the atmosphere to the seawater in the feed tank.SELECTED DRAWING: Figure 1

The application discloses a kind of micro invertebrate automatic detection method, comprising the following steps: step S1: obtaining water sample;Step S2: sample concentration;Step S3: optical detection;Step S4: filter detection unit cleaning;Step S5: detect filter detection unit filter membrane pollution;Step S6: replace filter detection unit;Step S7: manually replace filter detection unit;The application is concentrated sample for detecting chironomid larvae by realizing automatic filtration collection to water sample in step S2, detects chironomid larvae in concentrated sample in filter detection unit by sample imaging unit in step S3, and filter detection unit is cleaned by step S4;Filter detection unit with filter membrane pollution is replaced by step S5 and step S6;The application does not need manual collection and detection chironomid larvae, reduces the cumbersome step of manual operation, and is simple to operate, step is simple, improves the frequency and efficiency of sample collection, sample detection.

To provide a feed for marine invertebrates such as abalones and urchins, to provide a method for producing the feed for marine invertebrates, to provide a method for farming marine invertebrates, to provide a method for accelerating the length of shells, and to provide a method for increasing the content of fatty acids.SOLUTION: Provided are: a feed for marine invertebrates, characterized by containing microalgae belonging to the genus Euglena; a method for producing the feed for marine invertebrates, characterized by performing a solidification step of solidifying microalgae belonging to the genus Euglena; a method for farming marine invertebrates and a method for accelerating the length of shells, which comprise feeding microalgae belonging to the genus Euglena to marine invertebrates; and a method for increasing the content of fatty acids, which comprises feeding microalgae belonging to the genus Euglena to increase the content of fatty acids contained in edible parts of marine invertebrates.SELECTED DRAWING: Figure 5

The utility model relates to the technical field of invertebrate breeding, and discloses an earthworm collecting device for earthworm breeding, which comprises an earthworm breeding box and pulleys arranged on the bottom surface of the earthworm breeding box, vent holes are arranged on two sides of the earthworm breeding box, a breeding collecting device is arranged in the earthworm breeding box, and the breeding collecting device is arranged in the earthworm breeding box. A placement plate is arranged above the breeding collection device, and a breeding induction device is arranged on the top surface of the placement plate. By arranging the two symmetrical earthworm breeding boxes and arranging the telescopic bases and the electric telescopic rods at the bottoms of the breeding boxes, when earthworms are collected, the two breeding boxes only need to be pulled open towards the two sides, and then a color temperature adjusting and controlling lamp and a water spraying plate on one side are controlled, so that the earthworms are attracted by the breeding box on the other side, move to an earthworm outlet and fall into a collecting box; the earthworms can be collected without arranging a suction box, the space in the device is saved, the breeding efficiency is improved, and the device is simple in structure, convenient to operate and high in practicability.

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.

The utility model discloses a high-flux small aquatic animal metabolic rate measuring device which comprises a test cylinder, a placing table, a breathing chamber and a breathing cup, a temperature controller is connected in the test cylinder, a main body pipeline is connected to the upper side of the placing table, a water pump is fixedly connected in the test cylinder, and a water conveying pipe is fixedly connected between the water pump and the main body pipeline; covers are arranged on the front side and the rear side of the breathing chamber, a round hole is formed in the exterior of the main body pipeline, a single-pass small pipe is fixedly connected to the exterior of the round hole, a flow stabilizing mechanism is installed in the breathing chamber, a water outlet pipe is fixedly connected to the exterior of the breathing chamber, a regulating valve is fixedly connected to the exterior of the water outlet pipe, and an oxygen dissolving instrument is installed in the breathing cup; the device can be used for measuring the metabolic rate of various aquatic invertebrates, tadpoles, fishes and other small aquatic animals, can realize high-flux measurement of a plurality of samples at the same time, has portability, is easy to assemble and carry, and can ensure the experiment precision by adjusting the water flow speeds of the pipelines to be relatively consistent.

The present invention provides an attachment base for sessile aquatic invertebrates that improves the structure of conventional coral attachment plugs and facilitates the promotion of growth and breeding management of sessile aquatic invertebrates in general. [Solution] The attachment substrate 10 for sessile aquatic invertebrates serves as a base for attaching sessile aquatic invertebrates, and includes a frame 1, an alkaline mineral plate 2 which is a member containing alkaline minerals and is placed on and near the attachment surface to which the sessile aquatic invertebrates attach, an internal magnet 3 which is a first magnetic piece and is placed inside the alkaline mineral plate 2, and an external magnet 4 which is a fixing member. The attachment substrate 10 for sessile aquatic invertebrates is fixed onto an in-aquarium shelf 7 which is a non-magnetic object placed between the frame 1 and the external magnet 4 via the attractive force between the internal magnet 3 and the external magnet 4.

The utility model discloses a marine invertebrate polysaccharide extraction device which comprises an extraction tank, a sealing cover is arranged at the top end of the extraction tank, and the top of the sealing cover is communicated with a feeding pipe. The marine invertebrate tissue can be subjected to efficient centrifugal separation, polysaccharide components can be fully released and separated, the extraction efficiency is improved, the extracted polysaccharide components can uniformly flow out through centrifugal holes, and a water bath heat preservation assembly accurately controls the temperature in the extraction tank through a circulating system of a heating water tank, a water pump, a heating coil and a backflow pipe. It is ensured that the polysaccharide extraction process is carried out under the optimal temperature condition, the stable temperature environment is beneficial to polysaccharide extraction and activity maintenance, the negative effects of temperature fluctuation on the extraction efficiency and the polysaccharide structure are avoided, the heating coils are evenly distributed in the heat preservation cavity, and the temperature stability and uniformity of the extraction process are ensured.

Low doses of fluralaner or another isoxazoline derivative are administered to an animal such as a pet, typically a dog or a cat, preferentially by administration of a repeated dose, at regular intervals or at varying intervals, each dose being of the same or different amount, which leads to efficacy against arthropods, especially fleas and ticks. Various regimens, galenic forms, posologies, therapeutic indications, combinations with other active or synergistic ingredients, and smart devices or methods for release of doses to the animal can be provided, alone or in combinations.

The invention discloses a device and method for invertebrate behavior ecology research, and relates to the technical field of animal behavior research, the device comprises a starting pipe, a heat insulation assembly and an environmental factor regulation and control assembly, and the heat insulation assembly is internally provided with a plurality of channels communicated with an inlet and an outlet; the inlet is connected with the starting pipe; the environmental factor regulation and control assembly comprises a temperature control assembly and at least two branch pipe groups, the branch pipe groups are in one-to-one correspondence with the outlets, and each branch pipe group is connected and communicated with each outlet; the temperature control assembly can adjust the temperature of each branch pipe group; and an environment stimulation source is placed in the branch pipe group. Single-factor environmental stimulation or multi-factor environmental stimulation is applied to the branch pipe groups, so that the environmental stimulation presented in different branch pipe groups is different; enabling the tested animal in the starting tube to autonomously move in the invertebrate behavior ecology research device; and obtaining selection behavior characteristics and data of the tested animal. According to the method, more systematic and comprehensive behavioral data can be obtained, and the experiment accuracy is improved.

The present invention discloses a field survey device and method for assessing the status of invertebrate habitats. The device comprises a first float, a bracket mounted on the first float, a winding device mounted on the bracket, a pull rope mounted on the winding device, and an in-water survey assembly mounted on one end of the pull rope. This technical solution places the float in the waters of the invertebrate habitat to be surveyed, allowing the in-water survey assembly to enter the waters. A wireless module transmits water quality data of the invertebrate habitat collected by a water quality detection sensor and image data of the invertebrate habitat collected by an image acquisition device to an external monitoring terminal for processing, allowing users to assess the status of the invertebrate habitat. This method can replace manual real-time collection of habitat status, reducing the difficulty of the survey and the intensity of manual labor.

The present invention relates to methods of isolating and / or culturing a population of mesangiogenic progenitor cells (MPCs) or cells of the endothelial lineage, wherein the methods comprise the use of marine invertebrate collagen. The invention also relates to compositions comprising MPCs and marine invertebrate collagen and medical uses of such compositions, and medical devices and kits comprising the same.

The present invention belongs to the technical field of benthic animal monitoring, and in particular to a method for monitoring large benthic invertebrates in different types of wadable rivers. The method provides a method for monitoring large benthic invertebrates in wadable rivers through five main steps, namely, classifying rivers by type, laying out monitoring points, selecting sampling areas, sampling, and counting the types and numbers of large benthic invertebrates in samples. The method can effectively and practically monitor large benthic invertebrates in different types of wadable rivers, and provide a reference and reference for carrying out benthic animal monitoring in different types of rivers.

The invention discloses multi-stage selection equipment for aquatic organisms, and relates to the field of aquatic organism selection, the multi-stage selection equipment comprises a selection box and a partition plate arranged in the selection box, the partition plate is provided with a plurality of bottom mud uniform laying devices, and one side of each bottom mud uniform laying device is provided with a plurality of secondary dilution uniform laying devices; and a plurality of suction selecting devices are arranged at the top of the inner wall of the selecting box. According to the selection equipment, efficient, accurate and automatic screening and collection of the benthic large invertebrates are achieved through collaborative design of modules such as bottom mud uniform laying, secondary dilution, suction selection and automatic discharging.

The utility model relates to the technical field of water ecological restoration, and provides an adjustable biofilm culturing device for improving nitrogen and phosphorus retention potential of a small and micro water body. The suspended ball filler on the biological filler filter frame and the MBBR filler in the suspended ball filler can provide good attachment conditions and habitat for periphytic algae, bacteria, fungi, invertebrates and the like, a periphytic biocenosis structure with rich biodiversity is constructed, and the biofilm biomass of the periphytic algae, bacteria, fungi, invertebrates and the like is improved by improving the biofilm biomass of the periphytic algae, bacteria, fungi, invertebrates and the like. The nitrogen and phosphorus retention capacity of the small and micro water body is improved; the height of the device in water can be adjusted according to the depth of a water body through the design of multiple embedded baffles of the fixed plate; according to the utility model, each group of suspended ball fillers can be freely detached, so that damaged suspended balls can be replaced or aged biological membranes can be updated; the material used by the utility model has the advantages of simplicity in manufacturing, low operation cost, large surface area, easiness in biofilm formation, easiness in cleaning, no harm to the environment and the like.

The utility model relates to a demersal invertebrate flotation device which comprises a flotation frame, an installation frame and a supporting frame, a first screen plate is arranged in a flotation cavity of the flotation frame, the flotation frame is sleeved with the first screen plate in cooperation with the installation frame, and an intercepting screen plate on the installation frame is aligned with the first screen plate. According to the screening device for the benthic invertebrates, due to the fact that the blocking net plate is arranged in the screening device, and the hole diameter of the blocking net holes is larger than that of the first screening net holes, when the benthic invertebrates are sorted, gravels and aquatic plant residues large in size can be blocked through the blocking net plate firstly, then further screening is conducted through the first screening net plate, non-target substances mixed in the benthic invertebrates can be effectively reduced, and the screening efficiency is improved. According to the benthic invertebrate collecting device, the possibility that benthic invertebrates are attached and hidden is reduced, the sorting difficulty is reduced, the sorting efficiency of the benthic invertebrates is improved, and therefore the collecting efficiency of benthic invertebrate samples is improved.

The utility model relates to the field of insect pest investigation and prevention, in particular to an invertebrate early warning or long-term monitoring device. And a support device. The container is a hollow circular truncated cone, the area of the bottom face of the hollow circular truncated cone is smaller than that of the top face of the hollow circular truncated cone, the top face is open, a wide edge wider than the wall thickness of the container is arranged on the edge of the top face, and a plurality of through holes are formed in the side wall of the container. The supporting device is a hollow cylinder with an opening, and two through holes which are in axial symmetry are formed in the side wall of the supporting device. A suspension device is arranged on the two through holes, and the early warning or long-term monitoring device can be suspended on a tree branch according to the requirements of a user so as to trap winged insects; in the using process, corresponding trapping liquid is added into the container according to the requirement of a user, the device is collected according to a sampling plan, and a new round of trapping can be started only by replacing a new container.

The invention relates to the technical field of biological enzymes, and discloses an N-acyl homoserine lactone degrading enzyme as well as a preparation method and application thereof. Wherein the amino acid sequence of the N-acyl homoserine lactone degrading enzyme is as shown in SEQ ID NO. 1. The N-acyl homoserine lactone degrading enzyme disclosed by the invention is derived from a co-epiphytic bacterium Pseudomonas onchidi XY-99 of a marine invertebrate organism, i.e., calf stone, has wide signal molecule degradation activity, can block a quorum sensing pathway of pathogenic bacteria and inhibit the generation of virulence factors and the formation of a biofilm, and can be used for preparing a product for preventing and controlling pseudomonas aeruginosa.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

The application provides a method for controlling filamentous algae pollution in water bodies based on biological control, which comprises the following steps: putting filter-feeding invertebrates into the water body to be controlled, so as to reduce the spores and biomass of early growth stages of filamentous algae; putting algivorous animals into the water body to be controlled, so as to feed on filamentous algae and inhibit the formation of algal mats of filamentous algae; and putting floating ecological carriers into the water body to be controlled, so as to induce filamentous algae to form algal mats on the floating ecological carriers. The application controls filamentous algae pollution in water bodies by comprehensively using filter-feeding invertebrates, algivorous animals and floating ecological carriers, the three have strong ecological synergy, can control the growth of filamentous algae in water bodies and improve water quality without relying on chemical agents through the interaction between multiple organisms, and has good environmental protection benefits and sustainability.

The present invention belongs to the field of animal disease-resistant breeding, and specifically relates to a method for breeding oysters with Vibrio resistance based on whole genome selection. In view of the problem that the existing whole genome selection technology is mostly used for vertebrates with specific immune functions, but the effect on invertebrates lacking specific immunity is not obvious, phenotypic evaluation and reference population construction are carried out in the summer when the disease breaks out, and individuals with the same genetic background, the same growth period and the same physiological stage as the reference population are selected as candidate parents. The optimal Bayes A model is used to estimate the genomic breeding value in the actual breeding process, thereby overcoming the problems that the existing genomic selection technology is directly applied to the breeding of oysters with Vibrio resistance, which cannot achieve the expected technical effect, and the problems such as reduced accuracy of genomic breeding value estimation, insufficient selection precision, and overall poor disease resistance of the selected oyster strains. This breeding scheme has higher selection accuracy for oysters.

The present invention belongs to the technical field of wetland ecological restoration technology, and relates to a method for restoring swamp invertebrate populations using Carex truncatum. The method comprises the following steps: first, selecting a degraded swamp area to be restored; second, collecting truncatum with mature Carex plants from a healthy swamp for transplanting; third, transplanting truncatum in a cluster pattern; and fourth, artificially excavating some depressions between dense groups of truncatum. The present invention constructs a truncatum micro-topography with water-land gradient characteristics by transplanting Carex truncatum, providing facultative habitats for aquatic and terrestrial invertebrates, thereby simultaneously restoring the two types of animal populations, improving overall biodiversity and the food supply capacity for water birds, and solving the fundamental problem that terrestrial animals in swamp wetlands have nowhere to live in the wet season and aquatic animals have nowhere to hide in the dry season.

This invention discloses a microneedle patch loaded with scallop-derived exosomes, its preparation method, and its application. The microneedle patch comprises microneedles and a substrate; the microneedles are biocompatible polymeric materials loaded with scallop-derived exosomes; the substrate is also a biocompatible polymeric material. The microneedle patch prepared by this invention has intact needle tips, high molding rate, and can effectively load exosomes and maintain their biological activity. The marine invertebrate-derived exosomes involved in this invention possess abundant biological activity, significantly promoting cell migration and proliferation. Combined with soluble microneedle transdermal drug delivery technology, it will accelerate wound healing and effectively prevent scar formation, demonstrating clear clinical translational potential.

The invention belongs to the technical field of benthonic animal monitoring, and particularly relates to a large benthonic invertebrate monitoring method capable of wading into different types of rivers, which comprises five main steps of river type division, monitoring point position arrangement, sampling area selection, sampling, and statistics of types and quantity of large benthonic invertebrates in samples. The method for monitoring the large benthonic invertebrates in the wading rivers can effectively and practically monitor the large benthonic invertebrates in different types of wading rivers, and provides reference for monitoring the benthonic invertebrates in different types of rivers.