The invention relates to a method for preparing antihypertensive peptides by fermenting marine organisms through probiotics. The method comprises the following steps: with marine organisms, such as
sea cucumber,
jellyfish, acaudina molpadioidea or sleeve-fish, rich in collagen, as raw materials, desalting, cleaning, carrying out pulping and
grinding treatment, and heating for killing microorganisms; cooling, adding
protease, carrying out
liquefaction treatment, carrying out
enzyme deactivation, adding glucose, and then putting into a
fermentation tank; sterilizing, inoculating a
probiotic leavening agent, fermenting, and filtering out residues; adding a
cryoprotectant to fermented filtrate, carrying out freeze-
drying to directly obtain a composite antihypertensive biological preparation containing active probiotics and bioactive peptides; and in addition, further carrying out
tubular membrane purification,
ultrafiltration membrane intercept classification and
nanofiltration membrane concentration and desalting on the fermented filtrate, and carrying out
spray drying or freeze-
drying to obtain antihypertensive
peptide products with relatively high purity. The marine organisms rich in collagen are adopted as the raw materials; the technical scheme of combining
protease liquefaction with
probiotic fermentation is adopted. Compared with a traditional enzymatic preparation technology, the method has the advantages that the preparation efficiency of high-activity antihypertensive
oligopeptide is improved and enhanced; and the preparation technology is reasonable and effective, and is high in
operability and suitable for industrialized production.