Processing method before imbedding biological specimen

Abstract

The invention relates to a processing method of biological specimens before embedment. The method is that the biological specimens is dipped into a processing solution prepared with formaldehyde withthe concentration of 37 to 40 percent per 37.5 to 42.5ml and 57.5 to 62.5g of carbamide for 1 to 3 days and then the specimens are transferred to place in an incubator of 30 to 40 DEG C for 1 to 4 hours and then the specimens are embedded with transparent urea-formaldehyde resin. The processing method has the advantages of effectively moisture removing, lower cost, simple operation, better embedment effect and anti-mildew, anti-pest and color preserving, and is especially applicable to the embedment of biological specimens with more water content, such as Flammulina, Craspedacusta, earthworms,small mollusks and fish, etc.

Description

technical field [0001] The invention relates to an embedding method of a biological sample, in particular to a treatment method of the biological sample before embedding. Background technique [0002] At present, the treatment method of biological specimens before embedding generally adopts direct dipping after drying specimens or embedding without any treatment. When this method is processed, the specimen with high water content is easily deformed during the drying process, and the original shape of the specimen cannot be fully expressed. The Chinese patent with application number 200510020434.9 discloses a method of plasticizing specimens with polyethylene glycol. This method uses different concentration gradients of polyethylene glycol for treatment. Although it can effectively remove water, the cost is high and the operation is relatively complicated. . Contents of the invention [0003] Aiming at the deficiencies of the prior art, the present invention provides a tr...

AI Technical Summary

Problems solved by technology

[0002] At present, the treatment method of biological specimens before embedding generally adopts direct dipping after drying specimens or embedding without any treatment. When this method is used for processing, the specimen with high water content is easily deformed during the drying process, and the original shape of the specimen cannot be fully expressed.

The Chinese patent with application number 200510020434.9 discloses a method of plasticizing specimens with polyethylene glycol. This method uses different concentration gradients of polyethylene glycol for treatment. Although it can effectively remove water, the cost is high and the operation is relatively complicated.