2260 results about "Oedogonium" patented technology

A high efficiency recirculating marine aquaculture process for producing fish at variable yield densities of up to 60 kg / meter3 of the aquaculture tank, including fish that spawn under short photoperiods such as gilthead seabream (Sparus aurata) as well as fish that spawn under long photoperiods such as striped bass (Morone saxatilis). The process involves broodstock conditioning, spawning / reproduction, larval growth, nursery, and adult grow-out operations. The process is characterized by high-rate growth at optimal process conditions requiring less than 10% daily water exchange, thereby enabling effluent waste from the system to be discharged to a municipal sewer after disinfection treatment, which in turn permits siting of the aquaculture process facility in urban / suburban locations where aquaculture processes have been previously infeasible.

The invention provides systems and methods for producing biofuel from algae wherein the algae and fishes are co-cultured in a body of water. The methods further comprise inducing the algae to accumulate lipids by environmental stress, and concentrating the algae prior to extraction of the algal oil. The systems of the invention comprise at least one growth enclosure, means for concentrating algae, and means for subjecting algae to environmental stress.

Provided herein are systems and methods for producing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and / or derivatives and / or mixtures thereof by growing algae that produce the oils containing EPA and / or DHA and / or derivatives and / or mixtures thereof, harvesting the algae with fish in one or more enclosed systems, and then processing fish to separate and purify the EPA and / or DHA. The multi-trophic systems provided herein comprise at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. Also provided herein are the lipid compositions extracted from the fishes.

The present invention belongs to an industrialization aquiculture field, particularly relates to a closed cycle water industrialized fish cultivating system. The system separates solid and liquid in a storage sedimentation basin and a backwater channel sedimentation and rotational flow pool sedimentation, the tiny particulate matter is separated and purified by a sandrock filtering bed of a one level biological filter based on the water particulate matter separation, the water purified by the one level biological filter is feed into a two stage biological filter, the biological film of biologic filling material in the one level and the two stage biological filters is degraded with ammonia nitrogen and azote nitrous acid, the total purifying rate of the water particulate matter reaches more than 99%, the culturing water is added with oxygen mechanically or by liquid oxygen, finally the water is disinfected and sterilized through a ultraviolet ray sterilizer, and the clean ecological water is feed into a fish pool, thus the waste water of the fish pool may be purified and used in repeat. The fish-cultivating system implements completely closed cycle water industrialized fish cultivation, the fish is out of a disease or less, and the fish survival is more than 80%.

A scalable fish rearing raceway system is provided, incorporating a fish containment structure having two semi-circular end sections, and two or more parallel fish raceways, surrounding a central zone for housing water treatment systems and a secondary fish crop. Heavy particulates are eliminated from the main fish rearing channels by use of conical areas located at either end of the parallel elongated raceways. Continuous removal of dead or dying fish from the raceway is accomplished by means of a floating mortality catcher consisting of a screened ramp at the surface of the cones which continuously collect moribund and dead fish. Grading bars separate and move fish underwater to an adjacent raceway through a common fish transfer channel. This larger scalable fish production system substantially reduces the direct labor and capital costs associated with the production of fish as compared with conventional circular fish rearing tanks. Continuous removal of dead fish from the raceway is accomplished by means of a sinking fish mortality catcher consisting of a screened ramp at the entrance to the hydrocones which collect both moribund (sunken) and “long” dead (floating) fish. A similar device is mounted at the main drain box on the outside of the raceway to capture dead and floating fish. The continuous removal of dead and dying fish significantly reduces labor costs associated with the frequent manual removal of such fish and reduces the transmittal of infectious diseases by the immediate removal of the dead and dying fish.

The invention relates to a general-purpose composite Chinese herbal medicine immune enhancer for freshwater cultured fish, which is made of raw materials with the following weight ratios, based on the amount added per ton of feed: rhubarb 800-2000g; Sanqi 350-1000g; water Silybum 1200-2500g; Cassia 600-800g; Astragalus 600-2000g; Scutellaria baicalensis 500-900g; 500-1500g; Garlic 1000-2000g. After many tests in the laboratory, it has been proved that the general-purpose compound Chinese herbal medicine immune enhancer for freshwater aquaculture fish can improve the survival rate of a variety of freshwater aquaculture fish in the process of attacking viruses; The long-term breeding experiment of farmed fish feed proves that the general-purpose compound Chinese herbal medicine immune enhancer for freshwater farmed fish can effectively reduce the incidence of a variety of high-density farmed freshwater fish and improve the survival of the breed; the additive is safe, efficient, and has no side effects for long-term use. The breeding objects have strong vitality, rapid growth, bright body color, high feed conversion rate, and good breeding benefits.

Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian, avian, or piscian reproductive sperm cells. The compositions comprise a suitable reproductive cell media and a transforming growth factor, an insulin-like growth factor, or zinc, and, optionally, inositol, transferrin, or fructose, or combinations thereof.

A system and method of sustainable aquaponics that vertically integrates unique aquaponic system designs with alternative aquaculture fish feed sources, fingerling production methods, alternative aquaculture / farmed fish grow out models, and green energy sources that yield organic produce in the form of fruits and vegetables. A raceway system serves for grow-out throughout the warm and cold months. During the summer months, fish can be spawned and fed for steady growth, while during the winter months, the fish continue to grow at slower quite acceptable growth rates. The present invention derives its water from an open, natural, established pond or lake ecosystem to ensure that the raceways are adequately seeded with all of nature's good and bad bacteria and algae. The present invention can include winter quarters for the fish that is well-insulated and heated to ensure that the fish are comfortable and will continue growing and spawning throughout winter months.

The invention discloses a method for removing water eutrophication, which comprises the following steps of: investigating a community structure of aquatic organisms in water body, and finding a missing link or broken link in a food chain according to an investigation result; stocking predacious fishes in the water body, removing small trash fishes and herbivorous fishes from the water body by adopting a medicament mode or a manual fishing mode, and regulating the community structure of the fishes in the water body; planting submerged plants, floating plants and emergent aquatic plants in the water with the depth of 0 to 1.5m; and stocking filter-feeding fishes, filter-feeding shellfishes, detritus feeding benthonic animals and herbivorous fishes in the water body. The method for removing water eutrophication can realize the removal of the water eutrophication and ecological restoration for large-scale water body of which the parameters cannot be manually controlled, effectively inhibit the outburst of algal bloom and keep the stable quality of the water body.

The present invention discloses a composition and method of providing ionic forms or compounds of any combination of three metals to produce a product that can be used as a antimicrobial as defined by the U.S. Environmental Protection Agency (EPA) such as a hard surface disinfectant and as a foliar spray or water treatment for the control of various diseases in garden row, field and tree crops, on hard surface areas such as equipment from infected fields or in hospitals, homes, etc. as well as against a wide range of human, plant and animal diseases. Additionally, the composition of the present invention is able to be used to coat and otherwise treat and disinfect food products such as but not limited to fish and shellfish, meat, milk, poultry, eggs and irrigated crops (both food and nonfood crops) as well as non food products as defined in the EPA exemption from tolerance for copper and copper products. The composition may include inerts such as surfactants, detergents and buffers to adjust the pH. The composition remains soluble in water and is useful efficacious against the spread of bacteria, viruses and fungi and other pathogens within a source of water such as a reservoir or pool. Alternatively, the composition may be in aerosolized, misted, vaporized, fogged, humidified forms to produce micronized particles which are able to remain in suspension in the air for long periods of time in order to act on air-borne fungal spores and / or pathogens.

A controlled eutrophication system and process are disclosed. The system includes the combination of a partitioned aquaculture system in conjunction with an anaerobic digester. Wastewater containing pollutants, such as nitrogen and phosphorus, are fed to the partitioned aquaculture system. Algae within the system converts the pollutants into algal biomass. Fish populations, in turn, control the algal populations. The fish populations may then be periodically harvested for human or animal consumption. A polishing chamber is contained in the system in which aquatic organisms remove substantial amounts of the algae from batch fed additions of water. The water is then discharged to an external water source containing virtually no pollutants. In one embodiment, the biomass excreted by the aquatic organisms in the system are collected and fed to a digester. In the digester, the biomass is converted to a hydrocarbon gas and collected for its fuel value, while the liquid fraction is collected for its fertilizer value.

The invention provides a breeding method for knocking out fish MC4R gene by using a CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system. The method comprises the following steps: determination of MC4R gene targeted site, preparation of gRNA, in-vitro microinjection and knock-out verification, screening of MC4R-gene-knock-out fish, culture of hybrid fish, and the like. The breeding method is suitable for all commercial fishes, and can implement quick growth and breeding of fishes by knocking out the fish MC4R gene. Compared with the traditional breeding process, the method provided by the invention has the characteristics of high accuracy, low cost and short pure line acquisition time. Compared with the transgenic breeding, the gene knock-out can cause gene malfunction of the fish, and can not bring in any external gene, thereby avoiding the problem of transgenic safety.

The present invention belongs to the field of aquatic cultivation technology, and is one purified system for high density green cultivation of sea water and fresh water fish in a complexly closed circulation facility. The lone purified water system consists of cultivation pond, deposition pond, aerating pond and biologically purifying pond to constitute one complete circulation system. Each of the cultivation pond, deposition pond and biologically purifying pond is provided with blowdown pipeline to exhaust deposited solid dirt. The present invention has raised fish survival rate and growth speed.

The present invention relates to the technical field of biology, particularly to development and applications of a heat shock induced Cas9 enzyme transgene danio rerio. The present invention firstly provides a Cas9 enzyme expression vector, which utilizes a heat shock induced promoter HSP70 to drive the expression of a downstream Cas9 gene. According to the present invention, the Cas9 enzyme expression vector and Tol2 mRNA are co-injected into wild type danio rerio single cell fertilized egg, and selection is performed to obtain the heat shock induced Cas9 enzyme transgene danio rerio, such that the gene editing research of the CRISPR-Cas9 system in the danio rerio is successfully achieved, and the know-out of the MC4R gene in the transgene danio rerio is firstly achieved; and the Cas9 enzyme expression vector is further suitable for heat shock induced gene knockout, gene knock-in, gene expression modification and other applications of other fishes.

The invention provides a compound Chinese herbal medicine immunopotentiator for fish, containing by weight of: 40-50% of astragalus, 10-20% of angelica, 15-20% of glycyrrhiza, 5-10% of hawthorn, 5-12% of honeysuckle and 5-12% of isatis. Advantages of the invention is that: (1) the immunopotentiator of the invention has no adverse effect on palatability of feedstuff, (2) body immunity of culture fishes can be substantially enhanced, and immunoprotection rate to fresh water aquiculture fishes and marine culture fishes reaches to 65-71%, and (3) growth of pathogenic bacteria like escherichia coli in intestinal tract of culture fishes can be substantially restrained, growth of fish can be promoted rapidly, and weighting rate and disease resistance of culture fishes can be raised. The immunopotentiator is suitable for various of culture fishes, has a broad future, and is easy for industrialization.

The present invention relates to a pond cultivation combination system with water-saving and safety function, belonging to the field of aquaculture technology. Said system is composed of fish pond, artificial wet land and canal which are mutually communicated by means of water flow. The fish pond, artificial wet land and canal are successively series-connected so as to form the invented stereoscopic multi-stage utilized, closed and circulated pond cultivation combination system. It is characterized by that it can make waste water of fish pond be passed through artificial wet land and canal to purify water quality, increase dissolved oxyger and change static water of fish pond into microflow water to make cultivation so as to attain the goal of implementing high-effective cultivation of fishes and saving water resources.

The invention relates to a method for constructing a deep pool and a shoal in a straight river channel. In the method, a spur dike is arranged on the left bank side of the straight river channel, a bank slope on the opposite bank of the spur dike is protected by riprap, a diving guide wall is arranged on the left and right banks of the downstream side of the spur dike respectively, the discharge cross section of the river channel is shortened by the spur dike to increase the flow rate of water flow, the bottom of a river bed of a river reach between the diving guide walls on the downstream side of the spur dike is continuously scoured to naturally form the deep pool, and a dead water area or slow flow water area formed between the diving guide wall and the river bank becomes the shoal through long-term deposition. The method has the advantages that: the deep pool is naturally formed and becomes a rest place for aquatic organisms such as fishes and the like, and can become a shelter for the aquatic organisms such as the fishes and the like in a flood period; plants can be planted on the shoal, and the shoal can become an organism habitat different from the deep pool; and the complexity of a river channel structure and water conservation condition diversity can be increased or repaired, and river biodiversity, population composition and rare species conservation are facilitated.

The invention provides a deep sea net cage culture integrated platform which comprises culture net cage subsystems which perform the fish culture function and composed of anti-wind wave net cages, a power supply subsystem using solar energy and wind energy to generate electricity, a supporting platform for fixing the power supply subsystem and the culture net cage subsystems in the special space and an anchoring subsystem. The power supply sub-system is installed on the supporting platform; the net cages are connected with the supporting platform in a flexible connecting mode, the anchoring subsystem is composed of a power supply subsystem anchoring device and a net cage subsystem anchoring device. The supporting platform is further provided with feed storage rooms, bait feeding devices, monitoring devices and a communication device. The platform can realize automatic net cage fish culture production in a sea area under highoceanic condition, and has the characteristics of strong wind wave resistance, environmental protection, and high production efficiency and the like, and is a comprehensive platform for supporting sea water fish culture from near-shore to deep sea.

The invention discloses a waste recycling and tail water treatment method for water tank intensively cultured fishes. The method mainly comprises the following main steps: treating cultured tail waterin a culture pond, precipitating and recycling impurities in the tail water, accelerating tail water to precipitate through a flocculant in a sedimentation tank, and absorbing and utilizing nutritivesalt in the water through aquatic plant, degrading the precipitated culture tail water through aeration, degrading pollutant in the water mainly through a biological agent, ensuring that pollutant ina filter dam can be blocked by fine sand and floating dregs can be removed, and filtered culture tail water finally passes a biological purification tank and is purified again through plant and biological action, and finally discharging the tail water to the culture pond and external river channels separately according to different standards. The method has the advantages that the culture tail water can be effectively circularly utilized, polluted tail water is filtered successively and enters culture finally after meeting standards, recycled culture waste is effectively utilized for the biological and plant growth, and ecological culture circulation is performed.

This invention provides a vessel system and methodology that can be used to promote growth of phytoplankton in the oceans. Unmanned self-controlled wave-powered vessels are equipped with storage units for dispensing a fertilizer, and with sensors to monitor ocean conditions and effects. Fleets of vessels move autonomously by on-board processing of GPS and directional information, piloting a path that is coordinated by a central processing unit. The vessels travel through a defined target area, creating a detailed survey of chemical and biological characteristics that affect grown. The data are processed in a computer model to identify precise locations and precise amounts of fertilizer that will produce the best results. Projected benefits of fertilizing plankton include sequestering CO2 from the atmosphere, and enhancing the marine food chain to improve the fish stock in and around the treated area.

The invention discloses a multifunctional artificial fish reef, and aims to provide an artificial reef which is beneficial to formation of different flow field environments with upwelling, can raise nutrient substances deposited at the seabed, can improve the primary sea field productivity near the artificial reef and can provide sufficient feed to fishes. The multifunctional artificial fish reef comprises a reef body, an inner reef cavity and a plurality of inlets and outlets, wherein the inner reef cavity is arranged inside the reef body; the plurality of inlets and outlets are formed in the side surface of the reef body and are communicated with the inner reef cavity; the outer side surface of the reef body extends downwards from top to bottom towards the outer side of the reef body in an inclined manner; a partitioning plate which extends upwards and downwards is arranged inside the inner reef cavity.

A recirculated marine aquaculture system and process for growing crustaceans or other fish within a body of water. Water from the body of water is treated by stripping carbon dioxide and biological byproducts and by oxygenating the water. A water treatment unit may be provided to treat the water and provide movement to the body water. The water treatment unit can be configured to provide cavitation to the water, which aids in the removal of carbon dioxide and biological byproducts. Water may also be cycled through a deflocculation tank to reduce the floc of bacteria within the body of water to acceptable levels for optimal growth of crustaceans or other fish within a body of water. Using the system and the deflocculation tank has the advantage of significantly reducing the water exchange rates and even the water effluent, while maintaining acceptable water quality for growing crustaceans or other fish.

The invention relates to an ecological repairing method of eutrophicated water bodies in rivers and lakes and sediments, and provides a construction method of an organism structural system for synchronously repairing eutrophicated water bodies in rivers and lakes and sediments in situ by means of emergent aquatic plants, submerged plants, benthonic animals, fishes and microorganisms. The light enhancing oxygen-enriched organism structural system for synchronously repairing eutrophicated water bodies in rivers and lakes and sediments in situ has the double effects of synchronously and ecologically repairing polluted water bodies and sediments and providing habitats for aquatic organisms and microorganisms. The system comprises a lens reflective light enhancing surface water emergent aquatic plant survival area, a hollow prismoid free sliding middle water purifying area, a sediment-water interface submerged plant, a benthonic animal and fish survival area, a microorganism enriched intensified sediment purifying area and an oxygen-enriching fixed rod. The system provided by the invention can synchronously and ecologically repair the water bodies and the surface sediments, has the characteristics of good pollutant removing effect, stable and lasting treatment effect and the like, and is suitable for synchronously repairing water bodies in rivers and lakes and sediments in situ.

The invention provides an alga / fish mixed type three-dimensional ecological fish shelter and a feeding method of the fish shelter, wherein the fish shelter comprises an anchoring system, a fish shelter top consisting of a floating ball and a fixed frame, a fish shelter middle layer consisting of a horizontal grid and a fixed frame suspended with a middle spherical fish shelter, and a fish shelterbottom consisting of a horizontal grid and a fixed frame suspended with a bottom spherical fish shelter; and the fish shelter middle layer is respectively connected with the fish shelter top and the fish shelter bottom by a connecting rope and the lengthways crossed grid. The feeding method comprises the following steps of: shipping to a feeding sea area, firstly throwing a first anchor along with the direction of incident flow, sequentially throwing a second anchor by means of fair current, tying an anchor tail rope and a mark float at the back end of the anchor, dragging the anchor tail rope by means of fair current, tensioning a whole system, and completing feeding. According to the invention, the fish shelter can be used for culturing and proliferating the alga with different water depths, is taken as a perching and refuge occasion of the small size medium-upper layer fish and a large size medium-upper layer juvenile fish, and is simple in structure, and convenient to assemble andrecover, so that the novel artificial fish shelter can be provided for building an ecological marine ranch.

A method is provided for producing prey organisms such as Artermia and rotifers, for feeding aquacultural organisms in particular at the larval stage. The method comprises cultivating the prey organisms during at least part of their life cycle in an aqueous medium comprising at least one lipid component having a DHA content of at least 30 wt %. The enriched prey organisms preferably have a DHA content of at least 12 wt % of their total lipid content. The prey organisms are suitable feed for larvae of fish including halibut, turbot, bass, and flounder, and crustaceans and molluscs.

The invention discloses a method for ecologically poly-culturing fishes with different feeding habits in net cages of a shrimp culture pond. A plurality of net cages are arranged in the shrimp culture pond; one or more omnivorous fishes, filter-feeding fishes and carnivorous fishes are put into the net cages; shrimps are put outside the net cages; the putting density of the shrimps is reduced; and the fishes and the shrimps are cultured in a separated manner by the net cages. The method can be used for culturing the fishes and shrimps in a separated manner, so that the shrimps are prevented from being preyed by the fishes, the fish baits are sufficiently utilized, and the catching is easy.

The invention provides an aquatic product feed additive composition, a preparation method and application thereof. The composition comprises the following raw materials in parts by weight: 30-45 parts of plant extract and 5-10 parts of montmorillonite, wherein the plant extract is prepared from the bulk pharmaceutical chemicals in parts by weight: 6-18 parts of wormwood, 6-18 parts of mintkit, 6-18 parts of astragalus root, 5-15 parts of white paeony root and 5-15 parts of fleece-flower root. The additive composition has the advantages of being good in palatability, enhancing animal appetite,improving fish intestinal tract functions, maintaining long-term health of fish alimentary canals, promoting digestive absorption of fishes, increasing fish organism immunocompetence, and strengthening health and stress resistance capacity; and after excrement enters a water body, water quality can be continuously improved, and problem of water degradation is relieved.

The present invention discloses an apparatus and a method for the ecological fishing cultivation based on the composite vertical flow artificial wetland; wherein, the apparatus adopts the water stream as the carrier among a cultivating fish pond, an artificial wetland, an ecological channel and a water compensation pond that are serially connected with each other; the cultivating fish pond is provided with a water pump and is communicated with the artificial wetland through a culvert pipe, the artificial wetland is connected with the ecological channel, and the communication position between the ecological channel and the cultivating pond is provided with a stream fall about 50 cm. The apparatus has simple structure, is convenient to cultivate the fish and obviously improves the cultivating yield, which protects the water environment while realizing the high-quality and high-efficient health cultivating of the fishes so as to realize a pond cultivating composite apparatus that can be sustainable to use.

An ecological fish-culturing system by use of the water drained from factory features that the reverse filter is used for the deposition pool to remove solid substance, the natural ore is used as the adsorbing, filtering and bio-membrane-attaching material, the novel artificial nunja and the appreciative plant growing in said nunja are used to degrade NH4, Cl and nitrate, the microbes are inoculated for biochemical reaction, and the resultant water is aerated for enriching oxygen gas.