The invention discloses a preparation method of a soft-shelled turtle granulated feed. The method comprises the following steps that 1, fish meal, chicken meal, beer yeast, bean pulp, flour and a mineral element mixture are mixed, smashed, blended, puffed and granulated under the temperature of 90-110 DEG C and dried under the temperature of 50-100 DEG C; 2, egg powder, wet sand insects and shrimp mince are mixed, homogenizing is performed through a colloid mill, and then glycine betaine, fish oil and vitamin mixtures are added and subjected to emulsification and homogenizing; 3, under vacuum, the emulsion obtained in the step 2 is sprayed to surfaces of semi-finished dried granules obtained in the step 1 in a vacuumized mode, and the feed is obtained through drying, cooling, smashing, screening, weighing and packaging. The method is suitable for large-scale production, the biological active components of fresh feed raw materials and instable nutrient elements such as fish oil and vitamins in the feed formula can be effectively kept, pathogens in the feed are reduced, the aquatic water quality environment can be improved, the food calling performance and utilizing efficiency of the feed can be improved, and the soft-shelled turtle survival rate and growth performance can be improved.