17 results about "EGG SHELL MEMBRANE" patented technology

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The invention discloses an eggshell membrane source antioxidant umami peptide and application thereof, the amino acid sequence of the eggshell membrane source umami peptide is at least one of CDDDF, CWDVYR and WHDCHR, and relates to the technical field of bioactive peptides. The eggshell membrane umami peptides with antioxidant activity are screened by using a method of combining virtual enzymolysis, machine learning, molecular docking and molecular dynamics simulation, and three eggshell membrane umami peptides are screened and can be combined with umami receptor T1R1 / T1R3 subunits, and the composite conformation is stable. Sensory evaluation and electronic tongue analysis prove that the eggshell membrane source antioxidant umami peptide has good umami characteristics, the umami threshold value is 0.04-0.10 mmol / L, the eggshell membrane source antioxidant umami peptide has DPPH free radical scavenging capacity, ABTS + free radical scavenging capacity and Fe < 3 + > reduction activity, a new raw material is provided for development of functional seasonings, and the eggshell membrane source antioxidant umami peptide has important application value.

The application provides a flexible functionalized eggshell membrane and a preparation method and application thereof. The preparation method comprises the following steps: dispersing graphene oxide in deionized water to form a graphene oxide dispersion solution, adding sodium borohydride as a reducing agent for reduction, and centrifuging to obtain reduced graphene oxide nano powder; washing the eggshell, placing the eggshell in white vinegar for ultrasonic treatment, peeling the eggshell membrane, rinsing the eggshell membrane with ultrapure water to remove residual substances, and then soaking the eggshell membrane in sodium hydroxide for ultrasonic treatment, and rinsing the eggshell membrane with deionized water to obtain a pretreated eggshell membrane; adding dopamine into Tris buffer solution and fully oscillating to obtain a polydopamine precursor solution; adding the pretreated eggshell membrane and the reduced graphene oxide nano powder into the polydopamine precursor solution, and oscillating for polymerization at a constant temperature to obtain the flexible functionalized eggshell membrane. The application functionalizes the waste eggshell membrane and converts the waste eggshell membrane into a material with high value, thereby providing a method for high-value recycling of the waste eggshell membrane, and the application has great value.

The invention belongs to the technical field of polypeptide preparation and biological pharmacy, and particularly relates to eggshell membrane polypeptide with thrombin inhibition activity and application of the eggshell membrane polypeptide. According to the invention, two polypeptides are separated and purified from an eggshell membrane, and the two polypeptides are a nonapeptide YDGQHGRVS and an octapeptide EVPPSAL respectively. Tests prove that the nonapeptide YDGQHGRVS and the octapeptide EVPPSAL have thrombin inhibition activity equivalent to that of positive control heparin sodium, and discover that the nonapeptide YDGQHGRVS prolongs the activated partial thromboplastin time and the prothrombin time. The nonapeptide YDGQHGRVS and the octapeptide EVPPSAL provided by the invention are derived from eggshell membranes, are natural in source, good in thrombin inhibition activity and high in safety, and have a good application prospect.

The application discloses an ionic liquid functionalized eggshell membrane protein material and a preparation method and application thereof, and belongs to the technical field of biological piezoelectric materials. The eggshell membrane is pretreated and then grinded into powder; the eggshell membrane powder is treated with a NaOH solution and then cleaned to neutral; the eggshell membrane powder is dissolved in an aqueous solution of 3-mercaptopropionic acid and acetic acid to react, the pH is adjusted to an isoelectric point, and a precipitate is collected and dried to obtain soluble eggshell membrane; the soluble eggshell membrane is added into ionic liquid and fully stirred and dissolved to obtain an eggshell membrane protein solution; dialysis and freeze-drying are performed to obtain ionic liquid functionalized eggshell membrane protein; and the ionic liquid functionalized eggshell membrane protein is redissolved in an aqueous solution, subjected to static defoaming, dried, and immersed in anhydrous ethanol for cross-linking to obtain an ionic liquid functionalized eggshell membrane protein film. The eggshell membrane protein film prepared by the application has good mechanical properties, high thermal stability and piezoelectricity, and the degradable protein material can be applied to the fields of biological tissue engineering, intelligent sensing and energy collection.

The invention relates to a pharmaceutical composition for reducing dentin hypersensitivity and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises chanterelle extract, rabdosia rubescens leaf extract, eggshell membrane protein peptide and bifidobacterium thermophile fermentation extract; the cantharellus cibarius extract is obtained by sequentially performing enzymolysis on cantharellus cibarius through compound enzyme, ginger protease and serratipeptidase and purifying enzymatic hydrolysate through an AB-8 macroporous resin column. The rabdosia rubescens leaf extract is obtained by ultrasonically extracting rabdosia rubescens leaves with an ethanol water solution and purifying an extracting solution with a composite filler adsorption column. The eggshell membrane protein peptide is obtained by carrying out enzymolysis on a shell membrane by virtue of ginger protease and ficin and purifying an enzymatic hydrolysate by virtue of a G-25 sephadex column. The fermentation extract of the bifidobacterium thermophilus is obtained by purifying fermentation liquor of the bifidobacterium thermophilus through an SP agarose gel FF column. All the components are compounded according to a specific ratio and have a synergistic effect, so that the dentin hypersensitivity can be relieved safely in a multi-effect manner.

The present application provides a chitosan sulfate and oligopeptide-containing eggshell membrane extract and a preparation method thereof. The preparation method of the chitosan sulfate and oligopeptide-containing eggshell membrane extract comprises the following steps: adding water with a pH value of 7.5±0.2 into an eggshell membrane, then adding a mixed preparation containing alkaline protease, cellulase, pectinase and sodium sulfide, performing enzymolysis, filtering a crude product, and drying a filtrate, and the chitosan sulfate and oligopeptide-containing eggshell membrane extract is obtained. The chitosan sulfate and oligopeptide-containing eggshell membrane extract can be used as a low-cost high-protein raw material additive, can replace high-priced protein raw materials, and can improve the immune capacity of a body.

The present invention discloses an eggshell membrane nonapeptide and its use in preventing and treating osteoporosis, belonging to the field of biotechnology. The amino acid sequence of the eggshell membrane nonapeptide is shown in SEQ ID NO. 1, specifically: Val-Pro-Pro-Gly-Asp-Tyr-Leu-Phe-Gln. The eggshell membrane nonapeptide of the present invention can repair and improve the antioxidant capacity, differentiation activity, and bone formation capacity of osteoblasts affected by oxidative stress, providing a new therapeutic approach for preventing and treating osteoporosis caused by oxidative stress.

The present application belongs to the technical field of polypeptide preparation and biopharmacy, and particularly relates to eggshell membrane polypeptides with thrombin inhibitory activity and application thereof. Two polypeptides are obtained by separation and purification from eggshell membranes, which are nonapeptide YDGQHGRVS and octapeptide EVPPSPAL. It is proved by experiments that the nonapeptide YDGQHGRVS and the octapeptide EVPPSPAL have thrombin inhibitory activity equivalent to that of the positive control heparin sodium, and it is found that the nonapeptide YDGQHGRVS prolongs activated partial thromboplastin time and prothrombin time. The nonapeptide YDGQHGRVS and the octapeptide EVPPSPAL provided by the present application are derived from eggshell membranes, are natural in origin, have good thrombin inhibitory activity, high safety, and have good application prospect.

The application discloses an eggshell membrane peptide for enhancing bone density and a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of an eggshell membrane peptide for enhancing bone density, which comprises the following steps: vacuum freeze-drying eggshell membranes, crushing the eggshell membranes to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernatant; adding collagenase, performing enzymolysis, centrifuging to take supernatant, and obtaining a primary enzymolysis liquid; adding alkaline phosphatase, performing enzymolysis, centrifuging to take supernatant, and obtaining an enzymolysis liquid; performing ultrafiltration, and drying to obtain the eggshell membrane peptide. The application utilizes keratinase to break the cross-linking of keratin by enzymolysis, utilizes collagenase to release active peptides capable of stimulating the proliferation of osteoblasts by enzymolysis, further utilizes alkaline phosphatase to activate phosphopeptide calcium binding sites by enzymolysis, promotes the deposition of calcium salt, and provides the eggshell membrane peptide for enhancing bone density.

Disclosed in the present invention is a synergistic phytomedical mind and body wellbeing formulation involving a botanical composition with a dual approach to inhibit Cyclooxygenase (COX) and Lipoxygenase (LOX) enzymes that enhances nervine function for therapeutic intervention encompassing phytoceutical compositions and methods for treating inflammatory conditions, neurological and gut microbiome disorders. The formulation integrates a selected blend of natural ingredients with main active ingredients, including Stabilized Flax Meal, Cynatine® FLX, Sunflower Lecithin, Boswellia Extract, Turmeric / Curcumin C-3 Reduct®, Egg Shell Membrane, Green Oat Extract-Neuravena®, Hyaluronic Acid, Manganese Chelate and AstaReal® Astaxanthin are carefully chosen for their documented efficacy in addressing various dimensions of chronic pain and inflammation, as well as promoting joint / tissue health, gut health and cognitive function. Through synergistic action, these ingredients work in tandem to provide holistic relief from chronic inflammation, thereby improving quality of life without reliance on harsh pharmaceutical interventions.

The invention belongs to the technical field of glucose sensing, and discloses an enzyme-free glucose sensing electrode based on a porous biological membrane, a preparation method and application, and the sensing electrode comprises a graphite substrate, a conductive silver layer, an egg shell membrane layer, a platinum conductive layer and a ferroferric oxide magnetic nanoparticle layer from bottom to top. The graphite substrate and the egg shell membrane layer are conductively glued through the conductive silver layer, and the structural advantages of the three-dimensional porous microstructure of the egg shell membrane are fully reserved. The platinum conducting layer improves the conductivity of the electrode substrate, and achieves efficient electron transmission performance through the synergistic effect of the platinum conducting layer and ferroferric oxide serving as nano-enzyme. The three-dimensional porous microstructure of the egg shell membrane increases the specific surface area of the surface of the sensor electrode, can provide a stable and solidified substrate for a nano-enzyme catalytic material, greatly enriches the active sites of the sensor working electrode, and ensures rapid and accurate enzyme-free glucose sensing detection. Meanwhile, the sensing electrode has the advantages of being easy to degrade, high in biocompatibility, environmentally friendly and the like.

The present application relates to the field of eggshell membrane peptides, and provides an anti-aging eggshell membrane peptide, a solid-phase synthesis method and application thereof, an anti-aging eggshell membrane peptide composition and a preparation method thereof, wherein the anti-aging eggshell membrane peptide is a polypeptide with an amino acid sequence as shown in SEQ ID NO. 1. The amino acid sequence is LGPVGYPKLR, i.e. Leu-Gly-Pro-Val-Gly-Tyr-Pro-Lys-Leu-Arg. The present application screens an eggshell membrane peptide with good anti-aging effect from an eggshell membrane hydrolysate, and synthesizes a polypeptide with a corresponding amino acid sequence by using a solid-phase synthesis method, so that the industrialization of the anti-aging eggshell membrane peptide can be simply realized, and the eggshell membrane peptide can be applied to anti-aging products.

A novel biocompatible composite scaffold that has been designed to support the repair and regeneration of damaged or lost bone tissue in clinical applications. The scaffold is primarily made from two natural biomaterials: polysaccharides extracted from Padina pavonica — an eatable algi fungus — and the eggshell membrane (ESM), a thin biological layer located between the eggshell and the egg white.

The application relates to a method for improving the piezoelectric constant and piezoelectric performance of eggshell membrane protein, and relates to the field of protein extraction. The eggshell membrane protein-based material prepared in the application has good biocompatibility and air permeability, can adapt to complex deformation and more comfortably contact human skin; the eggshell membrane is treated by ultrasonic treatment and crosslinked by using glutaraldehyde, the molecular structure of the eggshell membrane protein is optimized, and the piezoelectric constant and piezoelectric performance of the eggshell membrane protein-based material are effectively improved; by improving the piezoelectric constant and piezoelectric performance of the eggshell membrane protein, the resource utilization of the waste eggshell membrane can be realized, which not only helps to reduce environmental pollution, but also can bring economic benefits and social benefits to related industries; in addition, the application provides a new high-performance material for the field of material science, which not only helps to promote the progress and development of material science, but also helps to promote the innovation of wearable devices and sensors and realize the resource utilization of waste.

This invention discloses a method for promoting the dissolution of eggshell membrane proteins and preparing chelated calcium through high-pressure crushing. The method includes the following steps: separating and drying the eggshell and eggshell membrane, then pulverizing them separately to obtain eggshell powder and eggshell membrane powder; adding water to the eggshell membrane powder and homogenizing under high pressure, followed by centrifugation to obtain an eggshell membrane solution; adding eggshell powder to the eggshell membrane solution, adjusting the pH to 1-3, and adding pepsin to react; after the reaction, adjusting the pH of the eggshell membrane solution to 6.5-7.5, and adding trypsin to react; after the reaction, inactivating the enzymes, centrifuging to collect the supernatant, adding anhydrous ethanol to the supernatant for alcohol precipitation, centrifuging to obtain the precipitate, which is chelated calcium. This invention overcomes the technical difficulty of dissolving eggshell membrane proteins, providing a new approach for the industrial extraction of soluble eggshell membrane proteins.

The invention discloses drinking water based on natural extracts and a preparation method thereof, and belongs to the technical field of drinking water, the drinking water is prepared from the following raw materials by weight: 91.3-94.5% of purified water, 2.6-3.7% of an eggshell membrane-tea leaf co-extract, 2.6-3.5% of sea buckthorn seed normal juice, and the balance sodium bicarbonate; a preparation method of the eggshell membrane-tea leaf co-extracting solution comprises the following steps: firstly carrying out rapid cooling and co-extraction on eggshell membranes and tea leaves to obtain an extracting solution and a solid product, and then adding cellulase and neutral protease into the solid product to carry out enzymolysis treatment to obtain an enzymolysis mixture; performing freezing-unfreezing circulation treatment on the enzymolysis mixture, performing fermentation treatment on the enzymolysis mixture, saccharomycetes and lactic acid bacteria in a fermentation tank, collecting fermentation liquor, and mixing the fermentation liquor with extract liquor to obtain eggshell membrane-tea leaf co-extract liquor. Natural components can be effectively extracted and stably fused into drinking water, the product is not prone to layering and precipitation, meanwhile, the drinking water is good in taste, the body weight is reduced by reducing the weight of fat in the body, and the fat reducing function is taken into account.