The invention discloses a collagen-based and high-transparency matrix for
cornea thickening and a preparation method of the collagen-based and high-transparency matrix. The preparation method comprises the following steps: assembling a collagen composite solution into a collagen matrix
implant on a set curvature mold through electrochemical deposition; the collagen composite solution is prepared from collagen, glacial
acetic acid,
hydrogen peroxide,
heparin and water; putting the obtained collagen matrix
implant and a mold into a cross-linking agent solution for cross-linking; cleaning the residual cross-linking agent after completion, separating the collagen matrix
implant from the mold, and placing the collagen matrix implant under an
ultraviolet light source for cross-linking to obtain a double-cross-linked collagen matrix implant; and soaking the double-crosslinked collagen matrix implant in a
nerve growth factor solution, and performing absorption and combination to obtain the collagen-based and high-transparency matrix for
cornea thickening. According to the invention, an electro-deposition
assembly technical scheme is adopted, the
diameter and thickness of collagen
assembly fibers are adjusted and controlled through
electric field energy, and the collagen
assembly fibers are orderly and regularly arranged, so that scattering and
refraction of light are reduced, the light
transmittance of
cornea is increased and can reach more than 90%, and the problem of light blocking caused by materials is effectively avoided. Operation is matched with
femtosecond equipment, operation is easy, consumed time is short, and trauma is small.