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This invention discloses a three-dimensional imaging method for biological tissue samples based on CUBIC clearing antibody staining, comprising: placing mouse brain tissue in CUBIC-1 solution for degreasing and dehydration; subjecting the degreased and dehydrated mouse brain tissue to sequential cell membrane permeation and blocking treatments; incubating the blocked mouse brain tissue with diluted primary antibody GFAP and secondary antibody Alexa488; washing the mouse brain tissue after two antibody incubations; placing the washed mouse brain tissue in CUBIC-2 solution for matching, and then embedding the mouse brain tissue in resin; placing the resin-embedded sample in a buffer bath, and acquiring images using a TDI-fMOST camera. This invention achieves overall staining of large biological tissue samples using CUBIC clearing antibody staining.

The application relates to a magnetic resonance imaging method. The magnetic resonance imaging method comprises the following steps: performing forward blood flow acquisition on a 3D volume of a detection object by using a TOF 3D sequence to obtain a first group of magnetic resonance signals; the detection object is a blood vessel; performing reverse blood flow acquisition on the 3D volume of the detection object by using the TOF 3D sequence to obtain a second group of magnetic resonance signals; and reconstructing the first group of magnetic resonance signals and the second group of magnetic resonance signals to obtain a magnetic resonance image of the detection object. In the method, the blood flow signal acquisition is performed twice, the blood flow saturation effect can be effectively reduced, the blood flow signal intensity of the whole 3D volume is homogenized, and the influence of the signal intensity fluctuation in the blood vessel on the magnetic resonance imaging is reduced.