Rapid tumor tissue discrimination method based on tissue surface lipid fingerprint spectrogram

Abstract

The invention discloses a rapid tumor tissue discrimination method based on a tissue surface lipid fingerprint spectrogram, and the method comprises the following steps: firstly, collecting lipid molecules on the surface of a tissue and keeping in-situ information of the lipid molecules; then, acquiring a lipid molecular characteristic fingerprint spectrum of the surface of the tissue by means of a laser desorption / ionization mass spectrometry platform under the condition of no matrix participation; then, establishing a discrimination model of various tumors based on a lipid characteristic fingerprint spectrogram of the surface of the tissue by combining a statistical analysis method, and judging subtypes of tumor tissues, normal tissues and various tumor tissues; finally, realizing the tissue molecular digital imaging without the slice by combining the mass spectrum imaging technology, and using the tissue molecular digital imaging to distinguish the tumor boundary; and the rapid tumor tissue discrimination method realizes rapid, efficient and high-accuracy clinical tissue identification, tumor subtype discrimination and tumor boundary imaging, improves the detection flux, and increases the stability of lipid molecule information on the surface of the tissue and the difference dimension in tumor tissue identification.

Description

technical field [0001] The invention relates to the technical field of precise molecular biopsy of clinical tissues, in particular to a rapid tumor tissue discrimination method based on tissue surface lipid fingerprints. Background technique [0002] Malignant tumor (cancer) is one of the important killers that endanger the life and health of people in China and even the world in today's society. Every year, there are more than 14 million cases of malignant tumors worldwide, and more than 8 million deaths caused by malignant tumors. In China, an average of 10,000 people are diagnosed with cancer every day, and the death rate exceeds that of heart disease and cerebrovascular diseases. These two major diseases. Surgical resection is one of the main treatments for most patients with malignant solid tumors, especially early and mid-stage patients. However, the overall high recurrence rate of malignant tumors after surgery is still a challenging problem in medicine, especially ...

AI Technical Summary

Problems solved by technology

However, most of the existing MALDI-MS-based lipid analysis on the tissue surface requires spraying of organic or inorganic nano-matrix, which greatly consumes the sample preparation time, because of the surface inhomogeneity caused by crystallization, the ion suppression effect is obvious, the stability of the lipid spectrum and poor repeatability

On the other hand, operations such as slicing of tissue samples are still necessary, which greatly reduces its "high-throughput" performance, and greatly reduces the feasibility of quickly and effectively identifying a large number of tissue samples

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