146 results about "Lipid molecule" patented technology

An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.

An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.

Naturally occurring lipids of vegetable and animal origin are broken into smaller molecules, and used as dewatering aids. The process of breaking the molecules include transesterification, interesterification, and saponification followed by acidulation. The modified lipid molecules can adsorb on the surface of the particles to be dewatered and greatly enhance their hydrophobicity, which will help increase the rate of dewatering and hence reduce cake moisture. The modified lipids are more effective dewatering aids than the naturally occurring unmodified lipids, possibly because they can more readily form close-packed monolayers of hydrophobes on the surface of the particles.

The invention discloses a nanometer medicine particle. The nanometer medicine particle comprises a hydrophobic polymer, a single-layer lipid molecule, an amphipathy macromolecular compound, at least one chemotherapy drug attached to the hydrophobic polymer, and at least one near-infrared photothermal conversion agent, wherein the hydrophobic polymer, the at least one chemotherapy drug attached to the hydrophobic polymer, and the at least one near-infrared photothermal conversion agent form a hydrophobic core; the single-layer lipid molecule surrounds the surface of the hydrophobic core to form an intermediate layer; and the amphipathy macromolecular compound intersperses in the intermediate layer to form a shell. The invention also provides a preparation method of the nanometer medicine particle. The nanometer medicine particle can realize the combined treatment of thermotherapy and chemotherapy and has high biocompatibility; and the preparation method is simple, convenient and easy to carry out.

The invention discloses a novel cationic liposome, a cationic liposome nucleic acid pharmaceutical preparation having the same as well as a preparation method and application thereof. The cationic liposome comprises cationic lipid, assisted lipo, polycarboxyl betaine lipid molecule and a freezing protective agent. The cationic liposome nucleic acid pharmaceutical preparation comprises cationic liposome modified by neutral lipid of polycarboxyl betaine and nucleic acid pharmaceutical preparation working solution. According to the cationic liposome, the defects of high toxicity, bad blood stability and low transfection efficiency are overcome and the pharmaceutical effects of the nucleic acid drugs are effectively improved; and at the same time, as the preparation is undisturbed by blood serum during the use, the operations in the experiment and the treatment are simplified.

The invention relates to the measurement for the lipid molecule content in a human blood sample as well as the preparation and the application of a colon cancer or rectum cancer diagnosis reagent kit, in particular to the extraction of lipid molecules in a human blood sample and the content measurement thereof as well as the definition of colon cancer or rectum cancer lipid molecule marks. In addition, the invention also discloses the details about the detection of a few colon cancer or rectum cancer lipid molecule marks in a blood sample and provides a reagent kit which is formed according to the lipid molecule marks and used for colon cancer or rectum cancer diagnosis. According to the content of the lipid molecule marks in the sample, the possibility for a patient suffering the colon cancer or rectum cancer can be diagnosed from the sample through special calculation and analysis, and the invention also provides a new means for the healing efficacy analysis in the colon cancer or rectum cancer treatment.

A method is disclosed for encapsulating plasmids, oligonucleotides or negatively-charged drugs into liposomes having a different lipid composition between their inner and outer membrane bilayers and able to reach primary tumors and their metastases after intravenous injection to animals and humans. The formulation method includes complex formation between DNA with cationic lipid molecules and fusogenic/NLS peptide conjugates composed of a hydrophobic chain of about 10-20 amino acids and also containing four or more histidine residues or NLS at their one end. The encapsulated molecules display therapeutic efficacy in eradicating a variety of solid human tumors including but not limited to breast carcinoma and prostate carcinoma. Combination of the plasmids, oligonucleotides or negatively-charged drugs with other anti-neoplastic drugs (the positively-charged cis-platin, doxorubicin) encapsulated into liposomes are of therapeutic value. Also of therapeutic value in cancer eradication are combinations of encapsulated the plasmids, oligonucleotides or negatively-charged drugs with HSV-tk plus encapsulated ganciclovir.

The invention relates to a core-shell type nano medical granule targeting to tumor cells. The core-shell type nano medical granule comprises an inner hydrophobic polymer with chemotherapeutics medicines and epigenetics medicines in an adsorbing manner, a single-layer lipid molecule layer wrapping the inner hydrophobic core, and an hydrophilic outer shell targeting to the tumor cells. The invention further relates to a preparation method of the core-shell type nano medical granule and the application of the core-shell type nano medical granule to preparing anti-tumor medicines. The core-shell type nano medical granule combines the nano wrapping, the chemotherapeutics medicines and the epigenetics medicines, thereby having the advantages of improving the medicine delivery targeting, improving the sensitivity of the tumor cells to the chemotherapeutics medicines, suppressing the medicine resistance of the tumor cells, and the like.

The invention discloses a method for detecting lipid molecules in salmon. The method includes the following steps that firstly, a sample is processed in advance, wherein salmon meat is cleaned and ground into meal, an organic solvent I is added into the salmon meal to be shaken and evenly mixed, ultrasonic ice bath extraction is conducted, pure water is added after extraction to be shaken and evenly mixed, the obtained mixture is centrifuged by a freezing centrifugal machine, a bottom-phase solution is obtained, dried at low temperature and dissolved again by means of an organic solvent II, and a lipid crude extract is obtained; secondly, enrichment and purification are conducted, wherein a solid-phase extraction column is activated, then the lipid crude extract is subjected to sample loading, spraying and elution, and the collected elution solution is a lipid extraction solution; thirdly, the lipid extraction solution is subjected to mass spectrometric detection and analysis. By means of the method, structure verification and quantitative analysis of phosphatidylcholine (PC) and phosphatidyl ethanolamine (PE) in salmon can be achieved.

Lipidic compound (lipidic molecule)-telodendrimer hybrid nanoparticles. For example, the lipidic compound-telodendrimer hybrid nanoparticles are lipid/lipidoid-telodendrimer hybrid nanoparticles. The nanoparticles can comprise a plurality of lipidic molecules (e.g., lipid molecules, lipidoid molecules, or mixtures of different lipid molecules or different lipidoid molecules). The hybrid nanoparticles can comprise one or more lipid or lipidoid and one or more telodendrimer. The hybrid nanoparticles can also comprise cholesterol. In various examples, the hybrid nanoparticles also comprise a small molecule, peptide, protein, or a combination thereof. In various examples, lipid-telodendrimer hybrid nanoparticles comprising one or more small molecules or lipidoid-telodendrimer hybrid nanoparticles comprising one or more protein(s) and/or peptide(s) are used in methods of small-molecule or protein/peptide delivery.

The invention provides superparamagnetism nanoparticles, a preparation method therefor and applications. Each superparamagnetism nanoparticle comprises an oleic-acid-coated ferroferric oxide nanoparticle located in the core, PEG-modified lipid molecules coating the surface of the oleic-acid-coated ferroferric oxide nanoparticle and target molecules connected with PEG. PEG-modified lipid molecules are employed as a modification agent, iron-containing compounds are subjected to thermal decomposition, then the target molecules are connected onto PEG, and a superparamagnetism nanoparticle is obtained. The superparamagnetism nanoparticles raise stability and biocompatibility of magnetic nanoparticles in vivo. Through connection of the target molecules, the nano particle diameter of the product is decreased further, the particle size distribution scope is narrowed, further the stability of the product is raised, the targeting of the product is raised, and imaging effects of a formed superparamagnetism contrast agent in lesion tissues of the Alzheimer disease are improved.

The invention discloses a rapid tumor tissue discrimination method based on a tissue surface lipid fingerprint spectrogram, and the method comprises the following steps: firstly, collecting lipid molecules on the surface of a tissue and keeping in-situ information of the lipid molecules; then, acquiring a lipid molecular characteristic fingerprint spectrum of the surface of the tissue by means of a laser desorption/ionization mass spectrometry platform under the condition of no matrix participation; then, establishing a discrimination model of various tumors based on a lipid characteristic fingerprint spectrogram of the surface of the tissue by combining a statistical analysis method, and judging subtypes of tumor tissues, normal tissues and various tumor tissues; finally, realizing the tissue molecular digital imaging without the slice by combining the mass spectrum imaging technology, and using the tissue molecular digital imaging to distinguish the tumor boundary; and the rapid tumor tissue discrimination method realizes rapid, efficient and high-accuracy clinical tissue identification, tumor subtype discrimination and tumor boundary imaging, improves the detection flux, and increases the stability of lipid molecule information on the surface of the tissue and the difference dimension in tumor tissue identification.

The invention discloses a method for improving germination of stevia rebaudiana seeds, which relates to a crop seed treatment technique in the technical field of agriculture. The method comprises the following steps of: selecting and sterilizing seeds, preparing 6 to 8 percent aqueous solution of polyethylene glycol 4000, soaking the seeds into the prepared solution at the temperature of between 20 and 30 DEG C for 24 to 48 hours, then filtering the seeds, cleaning the seeds by using clear water, and airing the seeds for later sowing. In the method, the used polyethylene glycol is generally recognized that molecules of the polyethylene glycol can change biological membrane structures of various cells in cell engineering so that lipid molecules on a plasma membrane at a contact point of two cells are dispersed and recombined, can change the osmotic regulation capability of plants on plant physiology and are favorable for absorbing nutrition and inducing the activity of stimulation cells. The method can remarkably improve the capability of resisting adverse situations when the stevia rebaudiana seeds are germinated so that the stevia rebaudiana seeds still maintain higher germination rate and germination energy in adverse situation harm and the seedlings have good quality.

The invention discloses a GAG (glycosaminoglycan) analogue realizing cell membrane modification, a synthetic method of the GAG analogue and an application method of the GAG analogue in in-vitro induced stem cell directional differentiation. The GAG analogue can be inserted into the surface of a cell membrane and can prompt a stem cell to be directionally and efficiently differentiated into a mature neuronal cell in a shorter cycle. The phospholipid anchored GAG analogue is prepared firstly through RAFT (reversible addition-fragmentation chain transfer) polymerization, and then phospholipidation of the GAG analogue is realized through maleimidized lipid molecules; the phospholipidized GAG analogue is introduced to the surface of the stem cell through affinity interaction between phospholipid groups and the cell membrane, so that the stem cell can be better prompted to be efficiently differentiated into the mature neuronal cell. A synthetic process is simple, and a powerful means capable of realizing phospholipidation of almost every RAFT polymer is provided.

Compositions useful for reconstitution of concentrated formulations containing protein/peptide pharmaceuticals are provided. The composition generally includes one or more lipids, as well as one or more alcohols that promote and stabilize the formation of (a) lipid molecular assemblies with greater protein encapsulation; (b) protein-lipid complexes and (c) protein and lipid solutions. The reconstitution medium improves the protein-lipid association that in turn alters the pharmaceutical properties.

A method for preparing a nano-liposome food flavor comprises the steps as follows: a, 5-50 parts by mass of natural phospholipid molecules are dissolved in 100 parts by mass of an ethanol solution with mass concentration of 1%-20% to form a first solution; b, 5-30 parts by mass of polyethylene glycol modified lipid molecules are dissolved in 100 parts by mass of an ethanol solution with the mass concentration of 1%-20% to form a second solution; c, 20-80 parts by mass of polylactic acid and 10-80 parts by mass of food flavor are magnetically stirred and dissolved in 10-50 parts by mass of an organic solvent to form a third solution; d, the second solution and the third solution are slowly added in the first solution to obtain a food flavor solution; and e, the food flavor solution is subjected to ultrafiltration centrifugation and dialysis and then is homogenized or/and extruded by a high-pressure homogenizer or/and a high-pressure extruder so as to obtain the nano-liposome food flavor. The nano-liposome food flavor prepared with the method is stable in system, long in fragrance remaining time and high in fragrance concentration.

The invention relates to a lipid molecule, namely poly-carboxyl oxyneurine (R1-L-PCB), which is represented by the formula (I), and a preparation method and application thereof. The method for preparing the R1-L-PCB comprises the following steps of: reacting the lipid molecule with halogenated hydrocarbon to obtain R1-L-X, (II) for short; and then with the R1-L-X as an initiator and carboxyl oxyneurine CB as a monomer, performing polymerization reaction on an atom transfer radical to obtain the R1-L-PCB, (I) for short. The R1-L-PCB lipid molecule derivate has high nonspecific protein adsorption resistance performance, can be used in nonspecific protein adsorption of a liposome, is favorable for improving the stability and the transferring efficiency of the liposome and can be applied to the fields of medicines, cosmetics and the like.

The invention relates to a lipidosome nucleic acid vaccine adjuvant as well as a preparation method and application thereof. The lipidosome nucleic acid vaccine adjuvant is composed of cationic lipid, auxiliary lipid, polycarboxylic abromine neutral lipid and targeting molecule modified polycarboxylic abromine lipid molecules. The targeting molecules facilitate the system to target the immune cells; the cationic lipid supports the nucleic acid vaccine through electrostatic action; polycarboxylic abromine neutral lipid facilitates the escape of the nucleic acid vaccine from cell endosomes, so that the nucleic acid vaccine can enter cell nucleus for expression, and the nucleic acid is improved in presentation efficiency, generates an adjuvant effect, and greatly improves immune response. According to the invention, the problems of vaccination infection caused by inactivation failure during conventional attenuation and inactivation of the vaccine and the strict requirement for biosafety during the production process are solved; in addition, the lipidosome nucleic acid vaccine adjuvant provided by the invention is simplified in preparation technology and good in biosafety, and can be developed and applied to the prevention and immunity treatment of human serious diseases, such as AIDS and tumors.

The present invention discloses a simple method for identifying unknown lipid components in lipidomics based on CID fragmentation. The main technical route comprises: 1, establishing a lipidomics analysis and determination method; 2, establishing the lipidomics analysis and determination method by using a multi-stage fragmentation mass spectrometry hybrid high resolution mass spectrometer; 3, searching an online spectrum library according to the accurate mass number, and attributing the lipid component types corresponding to the lipid chromatographic peaks to be identified; 4, determining the total carbon content and the unsaturation degree of the fatty acyl part in the lipid molecule; 5, according to a simple formula, determining the characteristic fragment information of each chromatographic peak; 6, deriving the carbon number and the unsaturation degree of each fatty acyl group according to the obtained characteristic fragment information; and 7, finally determining the lipid molecule structure. According to the present invention, the method is accurate and sensitive, is independent of the standard compound, and can rapidly identify the structure of the unknown lipid molecule in the lipidomics data.

The invention discloses a kit for screening colorectal cancer, which comprises a device and a comparison card, wherein the device is used for detecting the content of phosphatide; the comparison card is established by utilizing the content of phosphatide; and the phosphatide is SPC (sphingosylphosphorylcholine), 14:0 LPC (lysophosphatidylcholine), 16:0 LPC, 18:2 LPC, 18:1 LPC, 18:0 LPC, 20:4 LPC, 20:0 LPC, 22:6 LPC and 22:0 LPC. A test of the kit proves that an effective early diagnosis molecule mark for the colorectal cancer is developed, a molecule detection model is established, the detection sensitivity and specificity can reach 88% and 80% according to the model, and a distinguishing effect for comparing the colorectal cancer with the normal state obtained by utilizing the lipid molecules is better than the one obtained by using the marks for researching the early diagnosis of the colorectal cancer in recent years.

The invention discloses a hydrophobic drug entrapped lipid-polymer with dual functions of targeting and radiosensitivity. The lipid-polymer comprises lipid molecules with a targeting function, a poly-nitroimidazole organic polymer with radiosensitivity and a hydrophobic anti-cancer drug, wherein the lipid molecules (DSPE-PEG-target) with the targeting function, soybean lecithin and DSPE-PEG are prepared from polypeptide and targeting molecules with the targeting function in the lipid molecules through chemical reactions, the hydrophobic poly-nitroimidazole organic polymer is prepared from functional groups, namely nitroimidazole, with the radiosensitivity under the action of polymerization, and the polymer is prepared from the targeting lipid molecules, the soybean lecithin, the poly-nitroimidazole organic polymer and the hydrophobic anti-cancer drug through hydrophilic/hydrophobic compounding. The radiosensitivity is improved, the synergetic treatment effects of radiotherapy and chemotherapy upon tumor are improved, and a toxic and side effect reduction function is achieved; and a synchronous treatment function of radiotherapy sensitizers and anti-cancer medicine molecules is achieved, and clinical practicability and practical treatment significances can be achieved.