The invention relates to a lipidosome
nucleic acid vaccine adjuvant as well as a preparation method and application thereof. The lipidosome
nucleic acid vaccine adjuvant is composed of cationic lipid, auxiliary lipid, polycarboxylic abromine
neutral lipid and targeting molecule modified polycarboxylic abromine lipid molecules. The targeting molecules facilitate the
system to target the immune cells; the cationic lipid supports the
nucleic acid vaccine through electrostatic action; polycarboxylic abromine
neutral lipid facilitates the escape of the nucleic acid vaccine from
cell endosomes, so that the nucleic acid vaccine can enter
cell nucleus for expression, and the nucleic acid is improved in presentation efficiency, generates an
adjuvant effect, and greatly improves immune response. According to the invention, the problems of
vaccination infection caused by inactivation failure during conventional attenuation and inactivation of the vaccine and the strict requirement for
biosafety during the production process are solved; in addition, the lipidosome nucleic acid
vaccine adjuvant provided by the invention is simplified in preparation technology and good in
biosafety, and can be developed and applied to the prevention and
immunity treatment of human serious diseases, such as AIDS and tumors.