60 results about "Lipid molecule" patented technology

The invention discloses a preparation method and application of bioactive lipid and lipid nanoparticles thereof. The bioactive lipid molecule is formed by bonding long-chain fatty acid (LCFA) with different chain lengths with two functional small molecules, namely phenylboronic acid pinacol ester (PBAP) and Tempol (Tpl) respectively, so that two types of lipid molecules, namely PBAP-LCFA and Tpl-LCFA, with anti-inflammatory activity are constructed. The lipids are used as functional components, and the anti-inflammatory lipid nanoparticles with good biocompatibility, including PLP, TLP and TPLP, can be prepared through a film dispersion method. The lipidoid and the lipid nanoparticles are simple and convenient in preparation process, controllable in structure and function and easy for large-scale production, and have treatment potential in various acute and chronic inflammatory diseases. In an animal model, the lipid nanoparticles have remarkable curative effects on acute peritonitis, acute lung injury, acute hepatic failure, asthma and other diseases. Besides, the lipid bilayer structure of the TPLP can efficiently entrap hydrophilic / hydrophobic drugs, can synergistically deliver treatment drugs while exerting the anti-inflammatory effect of the TPLP, and realizes a synergistic combined treatment strategy for chronic lung inflammation and other diseases.

The invention relates to a lipid molecule, and a composition and application thereof. The core structure of the lipid-like molecule covers a two-dimensional plane structure and a plurality of three-dimensional structures, and endows the LNP with adjustable membrane tissue characteristics and intracellular transport behaviors, so that a new strategy of adjusting delivery performance from the structural level is realized. In addition, part of the ionizable lipid is introduced into a fluorine substituent group, so that the internal hydrophobic interaction and colloidal stability of the LNP are improved, and the in-vivo delivery persistence is prolonged. The invention also provides a component-simplified LNP delivery system based on programmable regulation and control of ionizable lipid chemical bonds, which is different from the traditional strategy of needing to remove hepatic tropism components (such as cholesterol) or additionally introduce targeted regulation and control lipid (a fifth component) to realize organ targeting. Selective delivery of organs such as liver, spleen, lung, pancreas and the like can be realized under the condition of retaining cholesterol only by changing the type of a chemical bond between an aliphatic chain and a core structure in the ionizable lipid.

This invention discloses a cationic lipid molecule based on lipoic acid derivatives for mRNA delivery, its preparation method, and its uses. The cationic lipid molecule is prepared by reacting lipoic acid with a hydrophilic amine, wherein the hydrophilic amine is one of 4A1, 5A1, or 6A2. The cationic lipid molecule synthesized in this invention contains a protonated nitrogen atom that can bind to negatively charged nucleic acids and form highly stable nanoparticles with polyethyleneimine (PEI), thus improving nucleic acid loading and demonstrating its excellent application in mRNA delivery.

The present disclosure describes a lipid-polymer hybrid nanoparticle and a method of synthesizing such a nanoparticle or a composition containing such a nanoparticle. These nanoparticles are made from a biodegradable polymer-based micellar core surrounded by a lipid-based shell, wherein a majority of the agent is present on the inner periphery of such nanoparticles due to physical adhesion to lipid molecules. Only a small amount of medicament is encapsulated in the micelle core. Thus, the lipid-based shell becomes the primary excipient part of the nanoparticle, and the core containing the biodegradable polymer becomes the secondary excipient part of the nanoparticle.

The invention discloses a post-AMI heart failure oxidized lipid screening method based on solid phase extraction, LC-MS / MS and AI. The method comprises the steps of blood sample pretreatment, solid phase extraction and purification, LC-MS / MS detection and AI model screening. According to the method, high-sensitivity qualitative and quantitative detection of oxidized lipid is realized through optimized LC-MS / MS parameters, and key oxidized lipid molecules and risk levels of heart failure after AMI emergency treatment blood supply reconstruction can be quickly and accurately screened in combination with a trained random forest AI model; the kit solves the problems that in the prior art, trace oxidized lipid detection sensitivity is low, specificity is poor, and heart failure risk screening lags, has the advantages of being high in detection efficiency, good in accuracy and high in practicability, can provide a scientific basis for early warning and intervention of clinical heart failure after AMI, and has high clinical application value.

The invention discloses an imidazolyl lipid assembly as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: mixing diphenyl phosphate, alcohol and pyridine, and heating for reaction to obtain alkyl phosphate; mixing N, N-diethylethylenediamine, 5-imidazole formaldehyde and a solvent, carrying out a heating reaction in a protective atmosphere, then adding the alkyl phosphate, and continuing the heating reaction to obtain an imidazolyl lipid molecule; dissolving the imidazolyl lipid molecules in an organic solvent to obtain a mother solution; the imidazolyl lipid assembly is obtained from the mother liquor through a film hydration method. The imidazole-based lipid assembly constructed by the invention can utilize the imidazole group to destroy the normal structure of bacteria under the condition of no antibiotic load, and utilizes the catalytic action of the imidazole group to complete the destroy of a biological membrane matrix and an inflammation activation medium, so that the synergism of antibiosis and anti-inflammation is realized, and the imidazole-based lipid assembly has the effect of buffering local acidic pH.

The invention relates to an ionizable lipid molecule containing an arginine structure, a lipid nanoparticle containing the ionizable lipid molecule and application of the ionizable lipid molecule. Specifically, the invention provides an ionizable lipid molecule containing arginine and a derivative structure thereof as shown in a formula (1), a lipid nanoparticle containing the ionizable lipid molecule, and a preparation method and application of the ionizable lipid molecule. Compared with ionizable lipid molecules conventionally used in the field, the lipid nanoparticles prepared from the ionizable lipid molecules shown in the formula (1) can achieve high-efficiency delivery and expression of nucleic acid.

The invention belongs to the field of myocardial infarction diagnosis and research, and particularly relates to a serum lipid biomarker for predicting the recurrence risk of myocardial infarction and application of the serum lipid biomarker. The invention discloses a serum lipid biomarker for predicting the recurrence risk of myocardial infarction. The serum lipid biomarker for predicting the recurrence risk of myocardial infarction is PI (18: 2), PI (16: 0), TG (20: 2), PC (22: 4) and PI (22: 6). According to the method, through lipid deconstruction analysis with higher resolution, lipid molecules of a specific chain structure closely related to the PRMI risk can be identified, and more accurate prediction of the recurrence risk is realized. The deconstructed lipid molecular marker provided by the invention has higher specificity and reliability in clinical application, not only can make up for the defects of the traditional lipid marker, but also provides a brand new technical means and application prospect for early recognition and individualized intervention of AMI postoperative recurrence risk.

Provided are a peroxide-containing liposome, a preparation method therefor, and use thereof. The peroxide-containing liposome is a spherical vesicle having a double-layer membrane structure and formed by lipid molecules containing one or more peroxy groups, and the lipid molecules contain carbon-carbon double bonds. The peroxide-containing liposome can be obtained by mixing peroxide-containing lipid molecules with an aqueous system, wherein the peroxide-containing lipid molecules are obtained by reacting unsaturated lipid molecules containing -CH=CH- and / or -C≡C- with an additive in the presence of oxygen and a solvent, and the additive is selected from one or more of lipoxygenase, an oxidation catalyst, and an oxidizing agent. By means of in-vitro experiments in cells and in-vivo experiments in animals, it is found that the peroxide-containing liposome can kill tumor cells and can be used as a sensitizing agent in combination with other tumor treatment methods to effectively improve the therapeutic effect on tumors, thus having good application prospects in resisting tumors.

The present invention belongs to the technical field of biochemistry, and specifically relates to a phosphoramidite lipid molecule and a use thereof in an RNAi molecule. In the present invention, a series of lipid molecules containing phosphoramidite are prepared and reacted with oligonucleotides to prepare an RNAi molecule. The RNAi molecule can effectively target extrahepatic tissues, while not interfering with the effect of inhibiting a target gene. The lipid molecule prepared in the present invention has good prospects in extrahepatic delivery vectors, and the RNAi molecule prepared in the present invention has important value for the research and development and clinical application of RNAi drugs.

The application discloses preparation and application of lipid molecules and compositions thereof for delivering active ingredients, and relates to the field of biological medicine. The novel lipid molecules comprise general formula (I), (II) or (III): and can be used for delivering active ingredients (such as nucleic acids, polypeptides, proteins) to cells and / or organs. Embodiments of the application provide nucleic acid-lipid nanoparticle compositions of various novel lipid molecules, and a lipid nanoparticle delivery system composed of the same is used for delivering mRNA; the delivery efficiency is superior to that of the currently marketed product DLin-MC3-DMA at both a cell level and an animal level, and the lipid molecules can be used as a novel method for delivering nucleic acid drugs and promote development of nucleic acid drugs.

This invention provides a lipid compound, its preparation method and application, and a pharmaceutical composition, belonging to the pharmaceutical field. Through innovative design of the chemical structure of lipid molecules, particularly by specific modification of the hydrophilic head group and / or hydrophobic tail structure, this invention enables specific expression of mRNA in the liver, effectively avoiding off-target effects in non-target organs, and has significant scientific value and broad application prospects.

The invention discloses a cationic lipid compound as well as a preparation method and application thereof, and the cationic lipid compound has the following structural characteristics: 1, the lipid compound has a multi-amino amidino head structure, so that the delivery efficiency of nucleic acid molecules can be improved; 2, the lipid compound has a phenylamidine structure, so that the structural stability of amidino can be improved, and the physical and chemical stability of lipid molecules can be improved; and 3, double biodegradation structures based on amidino and amidino bonds on two sides of a benzene ring in a lipid compound structure can be hydrolyzed and broken into small molecules after nucleic acid molecules are effectively delivered, so that the cell biocompatibility of lipid molecules can be improved, and the in-vivo safety can be improved. In conclusion, the multi-amino amidino cationic lipid molecule has the characteristics of high delivery efficiency, good cell biocompatibility and excellent storage stability, provides more choices for nucleic acid drug delivery, and has important significance for development and application of nucleic acid drugs.

This invention discloses a fluorinated membrane-fused liposome for modifying immune cells to regulate immunity, its preparation method, and its applications, belonging to the field of biomedical technology. This invention synthesizes a series of lipid molecules with different fluorinated chain lengths, including DOPE-F3, DOPE-F5, DOPE-F7, DOPE-F9, DOPE-F11, and DOPE-F13. Fluorinated membrane-fused liposomes are then prepared using these molecules in combination with other lipid components via a thin-film hydration method. These fluorinated membrane-fused liposomes exhibit excellent anti-protein adsorption capacity, efficient membrane fusion properties, and active spleen targeting. Experiments show that these fluorinated membrane-fused liposomes can achieve efficient membrane fusion with immune cells and effectively accumulate in the spleen in vivo, overcoming the shortcomings of traditional liposomes such as easy liver retention and low endocytosis efficiency. This invention has broad application prospects in the field of immunocellular therapy.

The invention provides an ionizable lipid molecule, a preparation method and application thereof, a lipid nanoparticle and application thereof, and belongs to the technical field of medicinal chemistry. The invention provides an ionizable lipid molecule, the ionizable lipid molecule is a novel ionizable lipid molecule, and LNP prepared from the ionizable lipid molecule, non-cationic lipid, sterol and polyethylene glycol lipid can improve the delivery efficiency of a load nucleic acid drug and is low in toxicity. The data of the embodiments show that in both in vitro and in vivo delivery, it has an excellent ability to deliver nucleic acids into cells, and is significantly superior to the positive control (SM-102) amino lipids approved by USFDA.

This application relates to the field of biomedical engineering, specifically disclosing a stem cell preparation for treating acute ischemic stroke and its preparation method. The preparation comprises a carrier core formed integrally through membrane fusion and an engineered stem cell membrane shell. The carrier core is a homologous stem cell exosome loaded with reactive oxygen species (ROS) scavenging nanoenzymes and can anchor responsive hydrogel microspheres encapsulating neurotrophic factors. The engineered shell covers the core, and its surface is covalently linked to brain-targeting molecules and thermosensitive lipid molecules, the latter of which can expose the target molecules at body temperature for intelligent navigation. The preparation method includes stepwise preparation of the drug-loaded core and functionalized shell, followed by programmed freeze-thaw treatment to induce membrane fusion of the two into a complete preparation. The product of this application can efficiently target ischemic lesions and achieve sequential synergistic treatment by first scavenging ROS and then releasing trophic factors. Its preparation method has advantages such as modularity, mild conditions, and good preservation of bioactivity.

The present application relates to ionizable lipid molecules containing a tocopherol structure, lipid nanoparticles comprising the same and uses thereof. Specifically, an ionizable lipid molecule containing a tocopherol and derivatives thereof structure represented by formula (1), lipid nanoparticles comprising the same, methods of preparation and uses thereof are provided. Compared with ionizable lipid molecules conventionally used in the art, the lipid nanoparticles prepared from the ionizable lipid molecules represented by formula (1) of the present application can significantly improve the delivery efficiency and expression of nucleic acids.

The application discloses a starch-lipid complex and a preparation method thereof, and relates to the field of modified starches. The preparation method comprises the following steps: homogenizing an ethanol solution and starch to prepare starch milk, stirring and adjusting the pH to 10-11, then adjusting to neutral to obtain treated starch milk, ultrasonic treatment, centrifugation and water washing to obtain treated starch; homogenizing the treated starch with water to prepare a suspension, stirring after adding lipids under the condition of a temperature of 80-100 DEG C, cooling and recrystallization to prepare the starch-lipid complex. The starch molecules are dispiraled into single helix structure by the ethanol and alkali liquor cooperated ultrasonic treatment, and a V-shaped hydrophobic helical cavity structure is formed, so that more binding sites are provided for the lipid molecules. Compared with the starch-lipid complex prepared by the traditional hydrothermal treatment method, the starch-lipid complex has higher complex index and anti-digestibility, and when added into food, the starch-lipid complex can provide satiety while reducing the blood glucose index, so the starch-lipid complex has good application prospect.

This invention provides an ionizable lipid molecule, its preparation method, and its application, as well as lipid nanoparticles and their applications, belonging to the field of medicinal chemistry technology. This invention provides an ionizable lipid molecule, a novel type of ionizable lipid molecule, LNP prepared by combining it with non-cationic lipids, sterols, and polyethylene glycol lipids, which can improve the delivery efficiency of nucleic acid drugs loaded with it and has low toxicity. Data from the examples show that it has excellent ability to deliver nucleic acids into cells in both in vitro and in vivo delivery, and is significantly superior to the USFDA-approved positive control (SM-102) aminolipids.

This invention discloses a method for screening key lipids during buckwheat storage based on lipidomics and machine learning, belonging to the field of food quality detection and metabolic biomarker screening technology. The method includes: collecting buckwheat samples under different storage conditions; obtaining volatile compound data through GC-MS analysis; acquiring lipidomics data of the buckwheat samples using lipidomics analysis; measuring quality-related indicators of the buckwheat samples; preprocessing the volatile compound data and lipidomics data; constructing a LASSO regression model to extract lipid molecules with non-zero coefficients as the first candidate set of key lipids; constructing a random forest regression model to calculate the importance score of each lipid molecule; and evaluating the predictive performance of key lipid molecules on the changing trends of marker volatile compounds. This invention is the first to combine LASSO regression and random forest regression for screening key lipids during buckwheat storage, overcoming the limitations of single methods, resulting in more scientific and reliable screening results, and providing a new method for revealing the molecular mechanism of buckwheat storage quality formation.

The invention discloses a lipid composition and application thereof as a characteristic marker of camellia oil and in identification of the camellia oil. The lipid composition is composed of 11 lipid molecules. The invention provides 11 characteristic lipids of the camellia oil, the characteristic lipids are used as detection markers to establish an identification and analysis method of the camellia oil, and detection personnel can quickly identify adulteration conditions of the camellia oil only by determining peak areas of the 11 characteristic lipids and combining the data analysis method established by the invention. The method is simple, convenient and rapid to operate, free of complex software, models and machine learning methods, high in identification result accuracy, good in sensitivity and suitable for large-scale real sample detection, and has popularization and application prospects.

The present invention provides a method for efficiently producing lipid particles that contain an active component and in which the surfaces of the lipid particles are conjugated with a ligand specific to a target cell. The method for producing lipid particles with a ligand conjugated to the surface thereof includes: (1) a step for mixing an organic solvent phase (a) containing a lipid component, an aqueous phase (b) containing an active component, and an organic solvent phase or an aqueous phase (c) containing a molecule (targeting lipid molecule) in which a ligand specific to a target cell and a lipid are covalently bonded; and (2) a step for purifying the mixture obtained in step (1).

The application relates to the technical field of biological medicine, and discloses a preparation method of a liposome with a pH response type membrane fusion effect and application of the liposome. The pyridium betaine-terminated lipid molecule provided by the application can be quickly and reversibly protonated in a weak acid microenvironment of a tumor, the surface charge of the liposome is changed from negative to positive, and the precise switch of the membrane fusion behavior is realized; the self-adaptive fusion liposome constructed based on the pyridium betaine-terminated lipid molecule successfully converts the non-specific toxicity risk of the traditional fusion liposome into a space-limited intelligent response, remains stable and inert in blood circulation (pH=7.4), and specifically activates the membrane fusion at a tumor site, realizes direct cytoplasmic delivery of a drug and avoids lysosome degradation; the STING nano agonist constructed by loading the nano composite of a STING pathway agonist and a divalent metal ion on the platform exhibits a strong pH-dependent anti-tumor immune activation effect in vitro and in vivo, and significantly inhibits tumor growth while the systemic toxicity is extremely low.

The present invention relates to a dendritic cell-mimicked nanostructure and a fabrication method therefor and, more specifically, to a nanostructure in which a shell including a cell membrane of dendritic cell-derived lipid molecules is introduced to a nanoparticle core in order to take advantage of the surface antigen-presenting ability of dendritic cells and which enables targeting without disappearance in vivo, thereby providing an effect of inducing an effective immune response.

The application discloses a kind of lipid molecules for nucleic acid delivery and liver microenvironment repair and application, belong to medical biotechnology field, and the lipid molecule is the compound shown in formula (1) or its pharmaceutically acceptable salt, it contains tocopherol or tocol trienol fragment and zinc ion coordination group in its structure, with the chemical and biological function of ionizable lipid and the antioxidant, anti-inflammatory activity of vitamin E and zinc.The lipid nanoparticle comprising the compound can efficiently deliver various nucleic acids, and while releasing in liver target, by eliminating active oxygen, inhibiting inflammatory pathways, actively improving liver microenvironment, reducing oxidative stress and fibrosis damage.The delivery system realizes the synergistic treatment concept of "carrier is medicine", and has wide application prospect in nucleic acid drug treatment and gene vaccine development in chronic liver disease, liver fibrosis, liver cancer and other diseases.

The application provides a kind of light-responsive lipid carrier based on amino acid structure derivation and a synthesis method thereof, by covalent coupling of natural amino acid molecules and light-responsive molecules, form a lipid molecule with light-responsive activity, and can be assembled to form a light-responsive lipid delivery carrier, design and develop a light-responsive lipid delivery carrier to meet the light-controlled drug release requirements of precision medicine. The carrier can improve the biological safety of the material through molecular design, and improve the photo-thermal conversion efficiency of the photosensitizer; on the other hand, it can be orderly assembled with phospholipid molecules, realize uniform and controllable distribution, and has significant metabolic safety and excellent targeted delivery performance. In addition, this method overcomes the drug loading limit problem in traditional drug loading method, realizes efficient and controllable loading of photosensitizer.

The invention discloses a method for screening lipid markers for obesity of children. The method comprises the steps of sample collection, lipid mixture extraction, lipidomics detection analysis and data processing and statistical analysis. According to the invention, lipidomics detection can be carried out on serum of an obese child through a high performance liquid chromatography-mass spectrometry technology, then differential lipid molecules in serum of the obese child and serum of a healthy child are screened by means of a statistical method, and correlation between the differential lipid molecules and obesity indexes is evaluated. And the screened differential lipid molecules are used as lipid markers for screening, early warning, predicting or assisting in diagnosing obesity of children.

The invention provides a composite immunologic adjuvant, and the composite immunologic adjuvant comprises an immunologic adjuvant auxiliary material and a biolipid. The biological lipid molecules are natural substances generated by microbial metabolism and comprise one or more of lipopeptide, glycolipid and phospholipid. Researches find that the novel composite immunologic adjuvant containing the biolipid component can promote the generation of effector cells and antibodies, enhance the immune response ability of organisms to antigens, enable the antigens to be easily phagocytized by antigen presenting cells, show better biocompatibility, improve the bioavailability of vaccines and improve the bioavailability of the vaccines. Furthermore, the toxic and side effects in the existing traditional immunologic adjuvant are reduced.

Provided herein are methods and compositions for transfecting cells or an organism comprising the cells for use as therapeutic, medicinal products, or consumption products or generating thereof. The compositions may comprise a saccharide, a nucleic acid molecule, and / or a lipid molecule. The compositions and methods may promote or facilitate uptake of a nucleic acid by the cells.

The invention discloses a zebra fish-based spatial resolution lipidomics method, and belongs to the technical field of biological medicines. The zebrafish-based spatial resolution lipidomics method provided by the invention comprises the following steps: separating a zebrafish trunk from yolk; sample pretreatment and chromatography-mass spectrometry detection. According to the method, yolk is sucked by a pipettor, trunks are cleaned in batches by a vortex instrument, after the trunks and the yolk are pretreated, a sample is subjected to lipidomics detection by an ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap high-resolution mass spectrometer, and further data analysis is performed. According to the method, the trunk and yolk samples which can be used for high-quality and high-coverage lipidomics analysis are obtained while high sample flux is maintained for the first time, and regionalized analysis of zebrafish embryo / larva lipid metabolism is realized. The method has the advantages of simple sample mechanism, low lipid signal interference, high sensitivity and the like, and can detect inrecognizable subtle changes and key lipid molecules in the whole sample.