99 results about "Lipid molecule" patented technology

A vaccine adjuvant, and a preparation method therefor and a use thereof. The vaccine adjuvant is a MA105 immunologic adjuvant, and comprises (1) QS-21:50 μg / ml to 300 μg / ml; (2) Poly I:C: 400 μg / mL to 3000 μg / mL; and (3) lipid molecules constituting a vector, the vector being a mixture of a cationic liposome and a neutral liposome.

The invention relates to an auxiliary lipid molecule containing a tocopherol structure, a lipid nanoparticle containing the auxiliary lipid molecule and application of the auxiliary lipid molecule. Specifically, the invention provides an auxiliary lipid molecule containing tocopherol and a derivative structure thereof as shown in a formula (1), a lipid nanoparticle containing the auxiliary lipid molecule, and a preparation method and application of the auxiliary lipid molecule. Compared with auxiliary lipid molecules conventionally used in the field, the lipid nanoparticles prepared from the auxiliary lipid molecules shown in the formula (1) can significantly improve the delivery efficiency and expression of nucleic acid. # imgabs0 #

The invention discloses a preparation method and application of bioactive lipid and lipid nanoparticles thereof. The bioactive lipid molecule is formed by bonding long-chain fatty acid (LCFA) with different chain lengths with two functional small molecules, namely phenylboronic acid pinacol ester (PBAP) and Tempol (Tpl) respectively, so that two types of lipid molecules, namely PBAP-LCFA and Tpl-LCFA, with anti-inflammatory activity are constructed. The lipids are used as functional components, and the anti-inflammatory lipid nanoparticles with good biocompatibility, including PLP, TLP and TPLP, can be prepared through a film dispersion method. The lipidoid and the lipid nanoparticles are simple and convenient in preparation process, controllable in structure and function and easy for large-scale production, and have treatment potential in various acute and chronic inflammatory diseases. In an animal model, the lipid nanoparticles have remarkable curative effects on acute peritonitis, acute lung injury, acute hepatic failure, asthma and other diseases. Besides, the lipid bilayer structure of the TPLP can efficiently entrap hydrophilic / hydrophobic drugs, can synergistically deliver treatment drugs while exerting the anti-inflammatory effect of the TPLP, and realizes a synergistic combined treatment strategy for chronic lung inflammation and other diseases.

The invention relates to a lipid molecule, and a composition and application thereof. The core structure of the lipid-like molecule covers a two-dimensional plane structure and a plurality of three-dimensional structures, and endows the LNP with adjustable membrane tissue characteristics and intracellular transport behaviors, so that a new strategy of adjusting delivery performance from the structural level is realized. In addition, part of the ionizable lipid is introduced into a fluorine substituent group, so that the internal hydrophobic interaction and colloidal stability of the LNP are improved, and the in-vivo delivery persistence is prolonged. The invention also provides a component-simplified LNP delivery system based on programmable regulation and control of ionizable lipid chemical bonds, which is different from the traditional strategy of needing to remove hepatic tropism components (such as cholesterol) or additionally introduce targeted regulation and control lipid (a fifth component) to realize organ targeting. Selective delivery of organs such as liver, spleen, lung, pancreas and the like can be realized under the condition of retaining cholesterol only by changing the type of a chemical bond between an aliphatic chain and a core structure in the ionizable lipid.

The present invention discloses a lipid prodrug nanoassembly based on dynamic covalent chemistry, and its preparation method and application, which belong to the fields of dynamic binding chemistry and nanotechnology. The present invention designs a dynamic covalent chemical lipid nano drug delivery system, which is mainly obtained by self-assembly of lipid molecules with a hydrophilic head, a hydrophobic tail and a dynamic covalent side chain. The molecule has an o-diphenol side chain group and can be combined with anti-cancer, antibacterial and antifungal drugs, and has good anti-tumor and antibacterial effects. The present invention constructs a lipid drug delivery platform through dynamic covalent chemistry for the first time, which has the advantages of responsiveness, fixed point, on-demand release, etc. in terms of function. It combines a variety of drugs such as anti-cancer, antibacterial and antifungal drugs with lipid molecules through dynamic covalent chemistry to improve the therapeutic effect of drugs.

The present invention provides novel ionizable cationic lipid molecules, lipid nanoparticles composed of these lipid molecules, neutral lipid molecules, cholesterol-like lipid molecules, and PEGylated lipid molecules, and compositions containing these lipid nanoparticles. These lipid nanoparticles, as delivery vehicles for active ingredients, offer advantages such as small and uniform particle size, high encapsulation efficiency, and high cell transfection efficiency, making them particularly suitable for delivering nucleic acid molecules (e.g., mRNA).

This invention discloses a cationic lipid molecule based on lipoic acid derivatives for mRNA delivery, its preparation method, and its uses. The cationic lipid molecule is prepared by reacting lipoic acid with a hydrophilic amine, wherein the hydrophilic amine is one of 4A1, 5A1, or 6A2. The cationic lipid molecule synthesized in this invention contains a protonated nitrogen atom that can bind to negatively charged nucleic acids and form highly stable nanoparticles with polyethyleneimine (PEI), thus improving nucleic acid loading and demonstrating its excellent application in mRNA delivery.

The present disclosure describes a lipid-polymer hybrid nanoparticle and a method of synthesizing such a nanoparticle or a composition containing such a nanoparticle. These nanoparticles are made from a biodegradable polymer-based micellar core surrounded by a lipid-based shell, wherein a majority of the agent is present on the inner periphery of such nanoparticles due to physical adhesion to lipid molecules. Only a small amount of medicament is encapsulated in the micelle core. Thus, the lipid-based shell becomes the primary excipient part of the nanoparticle, and the core containing the biodegradable polymer becomes the secondary excipient part of the nanoparticle.

The invention belongs to the technical field of biological medicine, and particularly provides a branched polyamidoamine lipid molecule, a nucleic acid targeted delivery system based on the branched polyamidoamine lipid molecule and a preparation method of the branched polyamidoamine lipid molecule, the branched polyamidoamine lipid molecule and the nucleic acid targeted delivery system based on the branched polyamidoamine lipid molecule are prepared by synthesizing branched lipid molecules PAMAM / S, PAMAM / Se and PAMAM / C with reduction potential adjustable groups S-S, Se-Se and C-C, optimizing the formula proportion of PAMAM / S: DOPC: Chol: DMG-PEG in LNP, and combining a microflow control preparation technology to obtain the branched polyamidoamine lipid molecule with the particle size of 80 nm and PDIlt; and 0.2 of stable nanoparticles. The delivery system specifically targets liver Kupffer cells, the EGFP transfection efficiency reaches 47.1% and is remarkably higher than that of traditional LNP, the cytotoxicity and immunogenicity are low, and the delivery system is suitable for development of mRNA vaccines and gene therapy drugs.

The invention discloses a post-AMI heart failure oxidized lipid screening method based on solid phase extraction, LC-MS / MS and AI. The method comprises the steps of blood sample pretreatment, solid phase extraction and purification, LC-MS / MS detection and AI model screening. According to the method, high-sensitivity qualitative and quantitative detection of oxidized lipid is realized through optimized LC-MS / MS parameters, and key oxidized lipid molecules and risk levels of heart failure after AMI emergency treatment blood supply reconstruction can be quickly and accurately screened in combination with a trained random forest AI model; the kit solves the problems that in the prior art, trace oxidized lipid detection sensitivity is low, specificity is poor, and heart failure risk screening lags, has the advantages of being high in detection efficiency, good in accuracy and high in practicability, can provide a scientific basis for early warning and intervention of clinical heart failure after AMI, and has high clinical application value.

The invention discloses an imidazolyl lipid assembly as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: mixing diphenyl phosphate, alcohol and pyridine, and heating for reaction to obtain alkyl phosphate; mixing N, N-diethylethylenediamine, 5-imidazole formaldehyde and a solvent, carrying out a heating reaction in a protective atmosphere, then adding the alkyl phosphate, and continuing the heating reaction to obtain an imidazolyl lipid molecule; dissolving the imidazolyl lipid molecules in an organic solvent to obtain a mother solution; the imidazolyl lipid assembly is obtained from the mother liquor through a film hydration method. The imidazole-based lipid assembly constructed by the invention can utilize the imidazole group to destroy the normal structure of bacteria under the condition of no antibiotic load, and utilizes the catalytic action of the imidazole group to complete the destroy of a biological membrane matrix and an inflammation activation medium, so that the synergism of antibiosis and anti-inflammation is realized, and the imidazole-based lipid assembly has the effect of buffering local acidic pH.

The invention relates to an ionizable lipid molecule containing an arginine structure, a lipid nanoparticle containing the ionizable lipid molecule and application of the ionizable lipid molecule. Specifically, the invention provides an ionizable lipid molecule containing arginine and a derivative structure thereof as shown in a formula (1), a lipid nanoparticle containing the ionizable lipid molecule, and a preparation method and application of the ionizable lipid molecule. Compared with ionizable lipid molecules conventionally used in the field, the lipid nanoparticles prepared from the ionizable lipid molecules shown in the formula (1) can achieve high-efficiency delivery and expression of nucleic acid.

The invention belongs to the field of myocardial infarction diagnosis and research, and particularly relates to a serum lipid biomarker for predicting the recurrence risk of myocardial infarction and application of the serum lipid biomarker. The invention discloses a serum lipid biomarker for predicting the recurrence risk of myocardial infarction. The serum lipid biomarker for predicting the recurrence risk of myocardial infarction is PI (18: 2), PI (16: 0), TG (20: 2), PC (22: 4) and PI (22: 6). According to the method, through lipid deconstruction analysis with higher resolution, lipid molecules of a specific chain structure closely related to the PRMI risk can be identified, and more accurate prediction of the recurrence risk is realized. The deconstructed lipid molecular marker provided by the invention has higher specificity and reliability in clinical application, not only can make up for the defects of the traditional lipid marker, but also provides a brand new technical means and application prospect for early recognition and individualized intervention of AMI postoperative recurrence risk.

Provided are a peroxide-containing liposome, a preparation method therefor, and use thereof. The peroxide-containing liposome is a spherical vesicle having a double-layer membrane structure and formed by lipid molecules containing one or more peroxy groups, and the lipid molecules contain carbon-carbon double bonds. The peroxide-containing liposome can be obtained by mixing peroxide-containing lipid molecules with an aqueous system, wherein the peroxide-containing lipid molecules are obtained by reacting unsaturated lipid molecules containing -CH=CH- and / or -C≡C- with an additive in the presence of oxygen and a solvent, and the additive is selected from one or more of lipoxygenase, an oxidation catalyst, and an oxidizing agent. By means of in-vitro experiments in cells and in-vivo experiments in animals, it is found that the peroxide-containing liposome can kill tumor cells and can be used as a sensitizing agent in combination with other tumor treatment methods to effectively improve the therapeutic effect on tumors, thus having good application prospects in resisting tumors.

Provided is a novel cell capture method.　The present technology provides a bioparticle capture method, etc., in which at least a capture step, for capturing a bioparticle having a lipid membrane using a lipid-molecule-modified nucleic acid having a branched structure and a plurality of modified lipid molecules, is performed, and the distance from a branch point of the branched structure to a hydroxyl group that binds a lipid molecule is 3 Å or more.

The present invention belongs to the technical field of biochemistry, and specifically relates to a phosphoramidite lipid molecule and a use thereof in an RNAi molecule. In the present invention, a series of lipid molecules containing phosphoramidite are prepared and reacted with oligonucleotides to prepare an RNAi molecule. The RNAi molecule can effectively target extrahepatic tissues, while not interfering with the effect of inhibiting a target gene. The lipid molecule prepared in the present invention has good prospects in extrahepatic delivery vectors, and the RNAi molecule prepared in the present invention has important value for the research and development and clinical application of RNAi drugs.

The application discloses preparation and application of lipid molecules and compositions thereof for delivering active ingredients, and relates to the field of biological medicine. The novel lipid molecules comprise general formula (I), (II) or (III): and can be used for delivering active ingredients (such as nucleic acids, polypeptides, proteins) to cells and / or organs. Embodiments of the application provide nucleic acid-lipid nanoparticle compositions of various novel lipid molecules, and a lipid nanoparticle delivery system composed of the same is used for delivering mRNA; the delivery efficiency is superior to that of the currently marketed product DLin-MC3-DMA at both a cell level and an animal level, and the lipid molecules can be used as a novel method for delivering nucleic acid drugs and promote development of nucleic acid drugs.

This invention provides a lipid compound, its preparation method and application, and a pharmaceutical composition, belonging to the pharmaceutical field. Through innovative design of the chemical structure of lipid molecules, particularly by specific modification of the hydrophilic head group and / or hydrophobic tail structure, this invention enables specific expression of mRNA in the liver, effectively avoiding off-target effects in non-target organs, and has significant scientific value and broad application prospects.

The invention provides a preparation and delivery method of ionizable cationic lipid for a lung targeting LNP system, which comprises the following steps: acquiring a pre-established ionizable cationic lipid molecular structure database, and calculating the pKa value of lipid molecules and charge distribution characteristics in an acid environment by adopting a molecular dynamics simulation method to obtain the ionizable cationic lipid molecular structure database. Obtaining pKa regulation and control capability parameters and surface charge distribution data of the lipid molecules; molecular structure characteristics are obtained from the candidate lipid molecule set, a genetic algorithm is adopted to optimize the component proportion of lipid nanoparticles, and simulation is carried out according to the uptake efficiency and transfection capacity of lung tissue cells to obtain optimized component proportion parameters; and according to the optimized component proportioning parameters, constructing a lung targeting model of lipid nanoparticles by adopting a Monte Carlo simulation method, and calculating the mRNA release efficiency in a lung tissue environment to obtain quantitative indexes of the lung targeting capability and the mRNA release efficiency.

The invention discloses a cationic lipid compound as well as a preparation method and application thereof, and the cationic lipid compound has the following structural characteristics: 1, the lipid compound has a multi-amino amidino head structure, so that the delivery efficiency of nucleic acid molecules can be improved; 2, the lipid compound has a phenylamidine structure, so that the structural stability of amidino can be improved, and the physical and chemical stability of lipid molecules can be improved; and 3, double biodegradation structures based on amidino and amidino bonds on two sides of a benzene ring in a lipid compound structure can be hydrolyzed and broken into small molecules after nucleic acid molecules are effectively delivered, so that the cell biocompatibility of lipid molecules can be improved, and the in-vivo safety can be improved. In conclusion, the multi-amino amidino cationic lipid molecule has the characteristics of high delivery efficiency, good cell biocompatibility and excellent storage stability, provides more choices for nucleic acid drug delivery, and has important significance for development and application of nucleic acid drugs.

This invention discloses a fluorinated membrane-fused liposome for modifying immune cells to regulate immunity, its preparation method, and its applications, belonging to the field of biomedical technology. This invention synthesizes a series of lipid molecules with different fluorinated chain lengths, including DOPE-F3, DOPE-F5, DOPE-F7, DOPE-F9, DOPE-F11, and DOPE-F13. Fluorinated membrane-fused liposomes are then prepared using these molecules in combination with other lipid components via a thin-film hydration method. These fluorinated membrane-fused liposomes exhibit excellent anti-protein adsorption capacity, efficient membrane fusion properties, and active spleen targeting. Experiments show that these fluorinated membrane-fused liposomes can achieve efficient membrane fusion with immune cells and effectively accumulate in the spleen in vivo, overcoming the shortcomings of traditional liposomes such as easy liver retention and low endocytosis efficiency. This invention has broad application prospects in the field of immunocellular therapy.

The invention provides an ionizable lipid molecule, a preparation method and application thereof, a lipid nanoparticle and application thereof, and belongs to the technical field of medicinal chemistry. The invention provides an ionizable lipid molecule, the ionizable lipid molecule is a novel ionizable lipid molecule, and LNP prepared from the ionizable lipid molecule, non-cationic lipid, sterol and polyethylene glycol lipid can improve the delivery efficiency of a load nucleic acid drug and is low in toxicity. The data of the embodiments show that in both in vitro and in vivo delivery, it has an excellent ability to deliver nucleic acids into cells, and is significantly superior to the positive control (SM-102) amino lipids approved by USFDA.

This application relates to the field of biomedical engineering, specifically disclosing a stem cell preparation for treating acute ischemic stroke and its preparation method. The preparation comprises a carrier core formed integrally through membrane fusion and an engineered stem cell membrane shell. The carrier core is a homologous stem cell exosome loaded with reactive oxygen species (ROS) scavenging nanoenzymes and can anchor responsive hydrogel microspheres encapsulating neurotrophic factors. The engineered shell covers the core, and its surface is covalently linked to brain-targeting molecules and thermosensitive lipid molecules, the latter of which can expose the target molecules at body temperature for intelligent navigation. The preparation method includes stepwise preparation of the drug-loaded core and functionalized shell, followed by programmed freeze-thaw treatment to induce membrane fusion of the two into a complete preparation. The product of this application can efficiently target ischemic lesions and achieve sequential synergistic treatment by first scavenging ROS and then releasing trophic factors. Its preparation method has advantages such as modularity, mild conditions, and good preservation of bioactivity.

The present application relates to ionizable lipid molecules containing a tocopherol structure, lipid nanoparticles comprising the same and uses thereof. Specifically, an ionizable lipid molecule containing a tocopherol and derivatives thereof structure represented by formula (1), lipid nanoparticles comprising the same, methods of preparation and uses thereof are provided. Compared with ionizable lipid molecules conventionally used in the art, the lipid nanoparticles prepared from the ionizable lipid molecules represented by formula (1) of the present application can significantly improve the delivery efficiency and expression of nucleic acids.

The invention relates to a polyethylene glycol lipid molecule containing a tocopherol structure, lipid nanoparticles containing the polyethylene glycol lipid molecule and application of the polyethylene glycol lipid molecule. Specifically, the invention provides a polyethylene glycol lipid molecule containing tocopherol and a derivative structure thereof as shown in a formula (1), a lipid nanoparticle containing the polyethylene glycol lipid molecule, and a preparation method and application of the polyethylene glycol lipid molecule. Compared with a polyethylene glycol lipid molecule conventionally used in the field, the lipid nanoparticles prepared from the polyethylene glycol lipid molecule shown in the formula (1) can significantly improve the delivery efficiency and expression of nucleic acid. # imgabs0 #

The present invention discloses a cationic lipid compound, its preparation method, and its application. The synthesis of the cationic lipid compound in the present invention is unrestricted in the choice of isonitrile and can be performed normally without the presence of a catalyst. The method for synthesizing the cationic lipid compound in the present invention is simple, inexpensive, and easily applicable. Furthermore, the synthesized lipid molecular structures are diverse, and the synthesis yield is high, exceeding 70%. Gene vectors prepared using the cationic lipid compound in the present invention exhibit good gene transfection efficiency and low cytotoxicity, and have potential for clinical application.

The application discloses a starch-lipid complex and a preparation method thereof, and relates to the field of modified starches. The preparation method comprises the following steps: homogenizing an ethanol solution and starch to prepare starch milk, stirring and adjusting the pH to 10-11, then adjusting to neutral to obtain treated starch milk, ultrasonic treatment, centrifugation and water washing to obtain treated starch; homogenizing the treated starch with water to prepare a suspension, stirring after adding lipids under the condition of a temperature of 80-100 DEG C, cooling and recrystallization to prepare the starch-lipid complex. The starch molecules are dispiraled into single helix structure by the ethanol and alkali liquor cooperated ultrasonic treatment, and a V-shaped hydrophobic helical cavity structure is formed, so that more binding sites are provided for the lipid molecules. Compared with the starch-lipid complex prepared by the traditional hydrothermal treatment method, the starch-lipid complex has higher complex index and anti-digestibility, and when added into food, the starch-lipid complex can provide satiety while reducing the blood glucose index, so the starch-lipid complex has good application prospect.

This invention provides an ionizable lipid molecule, its preparation method, and its application, as well as lipid nanoparticles and their applications, belonging to the field of medicinal chemistry technology. This invention provides an ionizable lipid molecule, a novel type of ionizable lipid molecule, LNP prepared by combining it with non-cationic lipids, sterols, and polyethylene glycol lipids, which can improve the delivery efficiency of nucleic acid drugs loaded with it and has low toxicity. Data from the examples show that it has excellent ability to deliver nucleic acids into cells in both in vitro and in vivo delivery, and is significantly superior to the USFDA-approved positive control (SM-102) aminolipids.

This invention discloses a method for screening key lipids during buckwheat storage based on lipidomics and machine learning, belonging to the field of food quality detection and metabolic biomarker screening technology. The method includes: collecting buckwheat samples under different storage conditions; obtaining volatile compound data through GC-MS analysis; acquiring lipidomics data of the buckwheat samples using lipidomics analysis; measuring quality-related indicators of the buckwheat samples; preprocessing the volatile compound data and lipidomics data; constructing a LASSO regression model to extract lipid molecules with non-zero coefficients as the first candidate set of key lipids; constructing a random forest regression model to calculate the importance score of each lipid molecule; and evaluating the predictive performance of key lipid molecules on the changing trends of marker volatile compounds. This invention is the first to combine LASSO regression and random forest regression for screening key lipids during buckwheat storage, overcoming the limitations of single methods, resulting in more scientific and reliable screening results, and providing a new method for revealing the molecular mechanism of buckwheat storage quality formation.