77results about How to "Great clinical value" patented technology

The invention provides a method and a system for predicting cardiovascular and cerebrovascular disease risks. The method comprises the following steps: step 1, defining problems of cardiovascular and cerebrovascular disease prognosis risk prediction, step 2, collecting health medical data of cardiovascular and cerebrovascular disease patients; step 3, preprocessing data, including data integration, data cleaning, and processing missing data; step 4, constructing features and selecting features, identifying potential risk factors; step 5, the identified risk factors and rehabilitation outcomes forming an input-output sample set, inputting the input-output sample set to a random forest algorithm for model training, and evaluating prediction performance of a model. The method and the system can obtain health medical data of cardiovascular and cerebrovascular disease patients, the data being required by a clinician input model, and a predicted rehabilitation outcome of a patient in a certain time period in future is obtained through the model. The method and the system can preferably predict prognosis risks, so as to realize personalized accurate rehabilitation treatment.

The invention discloses a method for preparing a meso-porous silicon nano medicine carrier with cell specificity target, reduction responsiveness and triple anticancer treatment effects. The method comprises the following steps: firstly, synthesizing meso-porous silicon nano particles by using a gel dissolution method, subsequently, introducing a disulfide bond onto the surface of a meso-porous silicon nano reservoir by using a chemical modification method, innovatively fixing cytochrome C with an apoptosis-inducing function onto the surface of the meso-porous silicon nano reservoir, blocking meso-porous channels with medicines, finally modifying DNA (Deoxyribose Nucleic Acid) aptamer single chain molecules (AS1411, with a cancer cell apoptosis-inducing function) onto the surface of a meso-porous silicon / cytochrome C nano composite system, and taking the system as specificity ligand of a receptor (nucleolin protein) which is overexpressed on the surface of liver cancer cytomembrane, thereby establishing a multifunctional composite type nano medicine carrier system for achieving triple anticancer treatment under combined action of medicines, blocking substances and target molecules inside meso-pores.

A method and instrument for detecting and analyzing the predication degree index of cardiacrhythm variability features that the predication degree is used as the index of detecting and analyzing cardiac rhythm variability. Said method includes such steps as using dynamic electrocardiograph to record cardioelectric signals, automatically recognizing dynamic cardioelectric RR gap, forming time sequence RRi, and calculating non-linear predication degree PI.

The invention discloses a rapid tumor tissue discrimination method based on a tissue surface lipid fingerprint spectrogram, and the method comprises the following steps: firstly, collecting lipid molecules on the surface of a tissue and keeping in-situ information of the lipid molecules; then, acquiring a lipid molecular characteristic fingerprint spectrum of the surface of the tissue by means of a laser desorption / ionization mass spectrometry platform under the condition of no matrix participation; then, establishing a discrimination model of various tumors based on a lipid characteristic fingerprint spectrogram of the surface of the tissue by combining a statistical analysis method, and judging subtypes of tumor tissues, normal tissues and various tumor tissues; finally, realizing the tissue molecular digital imaging without the slice by combining the mass spectrum imaging technology, and using the tissue molecular digital imaging to distinguish the tumor boundary; and the rapid tumor tissue discrimination method realizes rapid, efficient and high-accuracy clinical tissue identification, tumor subtype discrimination and tumor boundary imaging, improves the detection flux, and increases the stability of lipid molecule information on the surface of the tissue and the difference dimension in tumor tissue identification.

The invention discloses a preparation method of an individualized 3D printed high tibial osteotomy guide plate, for an osteotomy guide plate of a 3D printed high tibial osteotomy. Before a surgery, according to the CT data of the knee joint of a patient, through computer simulation modeling and a 3D reconstruction procedure, the deformity shape of the tibia shape of a patient is printed in proportion being 1 to 1, an osteotomy position is designed before the surgery through a 3D printing technique, and finally an osteotomy guider which is completely fitted to the near end of the tibia of the patient is designed through a model material; and in the surgery process, the osteotomy guider is directly fitted to the near end of the tibia in the surgery process, according to the preoperative osteotomy scheme, osteotomy surgery operations are immediately started, under regulation for guaranteeing accuracy, the surgery operation steps are simplified, the anaesthesia time of the surgery is shortened, the safety of the surgery of the patient is guaranteed, and the preparation method has great clinical promotion effects. A steel plate can be fixed according to tibia near ends provided by different factories, the position and the direction of the osteotomy of the tibia can be in individualized design, and the preparation method has definite diversified flexibility.

The invention provides an exosome-compounded collagen biological scaffold with a directional releasing function as well as a preparation method and application thereof. The exosome-compounded collagenbiological scaffold comprises nano collagen and exosomes dispersed in the nano-collagen. The preparation method of the biological scaffold comprises the following steps: separating collagen from animal tissue, carrying out ball milling till the collagen is nanoscale collagen; separating exosomes from culture of bone marrow or umbilical cord mesenchymal stem cells; mixing the exosomes with the collagen, freezing and drying. The preparation method of the biological scaffold is used for dispersing the exosomes in the nanoscale collagen to form a biological scaffold material with a function of directionally releasing exosomes; the targeted repairing and treating effects of the exosomes can be obviously improved; the development tendency of accurate drug administration can be met; the dosage forms are various; the biological scaffold has extensive clinical application values.

The invention discloses an application of an polypeptide in medicines for preventing or treating myocardial ischemia-reperfusion injury related diseases, and belongs to the field of biomedicines. The amino acid sequence of the polypeptide is SEQ ID NO.1 or SEQ ID NO.2. The polypeptide can substantially reduce the myocardial infarction area in myocardial ischemia-reperfusion injury, can be used as a medicine for preventing or treating the myocardial ischemia-reperfusion injury related diseases, and has clinic application values.

The invention provides a traction device bonded to impacted teeth. A ceramic bottom plate can be bonded onto the impacted teeth, a stainless steel plate is arranged on the ceramic bottom plate, a stainless steel ball is sunken in a concave ball groove of the stainless steel plate and can make rotary motion in the concave ball groove, a stainless steel rod is arranged at the top of the stainless steel ball, and an external thread is arranged on the upper half part of the stainless steel rod. A length adjusting unit comprises a stainless steel adjusting rod, the top of the stainless steel adjusting rod is inwards provided with an internal thread, the internal thread of the stainless steel adjusting rod is in matched connection with the external thread of the stainless steel rod, and a stainless steel ring is arranged at the bottom end of the stainless steel adjusting rod and connected with a fixed orthodontic appliance through an elastic chain or an auxiliary arch. The stainless steel adjusting rod can play a role in fixing and adjusting the length of the traction device so as to meet the requirement for traction of the impacted teeth at different depths. The traction device is smallin size, stimulation to surrounding tissue is little, and the comfort of a patient is good. Manufacturing is simple and standardized, and batch production is easy.

The invention relates to a construction method of replication-deficient recombinant adenovirus. Firstly, a double-stranded expression module of siGNA of the gene APE1 of human being is chemically synthesized and inserted under the prompter U6 of the shuttle plasmid of the adenovirus after restriction enzyme, then shuttle plasmid pDC316-EGFP-U6-APE1siRNA is constructed, and then shuttle plasmid pDC316-EGFP-U6-APE1siRNA and the bone plasmid of adenovirus pBHG-fiber5 / 35 are simultaneously cotransfected into cell 293, consequently the recombinant adenovirus Ad5 / F35-APE1siRNA with the expression sequence of siGNA of the gene APE1 of human being is achieved through the fixed-point recombination produced by the system function of Cre / loxP. The recombinant adenovirus is capable of effectively preventing the expression of the gene APE1 and effectively strengthening the sensibility of radiotheraphy and chemotherapy of tumour cell.

The invention discloses a high performance liquid chromatography tandem mass spectrum detection method of free catecholamine and metabolites in human urine. The method comprises the following steps of(1) preprocessing a sample, successively adding urine samples in an internal standard working solution and a phosphoric acid solution, and carrying out vortex mixing; (2) carrying out weak cation exchange solid phase extraction 96-well plate (SPE96 well plate) activation; (3) carrying out solid phase extraction processing, after step (1) processing, loading the samples to the SPE 96-well plate after step (2) activation, carrying out formic acid methanol solution elution, and drying eluant with nitrogen; and (4) redissolving and using a high performance liquid chromatography tandem mass spectrometry to carry out analysis detection. The method has the advantages of high accuracy, high reproducibility, high sensitivity, high specificity, high efficiency, high flux, low cost, simple and rapidoperation and the like.

This invention provides a method and a solution of separation and extraction of dissociative DNA in blood; it also provides a reagent box which is used to quantificational detecting of dissociative DNA in blood. This invention can detect the dissociative DNA in blood fast and sensitively and obtain the result.

The invention provides a traditional Chinese medicine composition for preventing urinary calculus, which comprises desmodium, waternut herb, semen abutili, ainsliaea spicata vant, nervilia fordii schltr, garden burnet, coix lachryma-jobi, calabash, securidaca inappendiculata hassk, chicken's gizzard membrane, lygodium japonicum, otolhum pseudoscinaenae, pyrrosia lingua, dianthus superbus, rhizome curcuma, gentrin knotweed, cassia twig, tree peony bark, fleece-flower root, cyathula root, plantain seed and licorice. Practice has proven that, the above traditional Chinese medicine composition is better in curative effect and small in toxic and side effect, thus being suitable for long-term administration. The traditional Chinese medicine composition is wide in wide in clinical application value. The traditional Chinese medicine composition serves as a safe and effective medicament used for treating urinary calculus.

The invention discloses a preparation method of a GdF3:Yb3+ and Er3+ to upconversion fluorescence nanomaterial. The preparation method comprises the following steps that (a) anhydrous ethanol, deionized water and a thiohydracrylic acid solution are mixed; (b) Gd(NO3)3.6H2O and Yb(NO3)3.5H2O powder is weighed and put in the mixed solution, and then a Er(NO3)3.5H2O solution is added; (c) an NH4F solid is weighed and added into the mixed solution in the step (b); (d) the liquid prepared in the step (c) is transferred to a polytetrafluoroethylene hydrothermal high-pressure reaction kettle, and continuous heating is performed at high temperature; (e) after the reaction kettle is cooled to room temperature, centrifugation is performed, and a precipitate is washed with ionized water and anhydrous ethanol and is dissolved in the deionized water; (f) the solution obtained in the step (e) is centrifuged, and the white precipitate is dried. Thiohydracrylic acid is used as a surface modification agent, GdF3:Yb3+ and Er3+ to upconversion nanoparticles are synthesized by adopting a one-step hydrothermal method, the varieties of modification agents for surface modification of upconversion nanoparticles are enriched, and a theoretical and experimental basis is provided for further probe establishment and targeted delivery of anti-cancer drugs.

A method and instrument for detecting and analyzing the variability of cardiac rhythm features that the Co mode degree PM is used as the non-linear analysis index of cardiac rhythm variability. Its method includes such steps as using dynamic electrocardiograph to record the cardioelectric signals within 24 hr, automatically recognizing the dynamic cardioelectric RR gaps, forming a time sequence RRi, and calculating non-linear Co mode degree PM.

The invention discloses a kit for detecting gene mutations in acute lymphoblastic leukemia based on fluorescence quantitative PCR. The kit comprises a PCR primer pair, a probe, a 2x PCR Nucleotide Mixreaction solution and RNase-Free ddH20. The kit has the advantages that a fluorescence quantitative PCR method is adopted to carry out specific amplification detection on PAX5 and IKZF1 gene mutationregion sequences, while high sensitivity and specificity are ensured, more mutation sites are considered. The detection kit provided by the embodiment is high in sensitivity, good in specificity andsimple to operate, and is a human PAX5 and IKZF1 gene mutation detection kit with excellent performance.

The invention discloses a high performance liquid chromatography detection method of free methoxy group noradrenaline and methoxy group in human plasma. The high performance liquid chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the human plasma comprises the following steps that: (1) sample pre-treatment is conducted, interior label working solution and spiritus mindereri are successively added into a plasma sample, vortex is mixed, and centrifugation is conducted; (2) weak positive ion exchange solid phase extraction 96 pore plate (SPE 96 poreplate) is activated; (3) solid phase extraction treatment is conducted, after the step (1) is conducted, the sample is loaded into the treated SPE 96 pore plate, a formic acid acetonitrile solution is eluted, and eluent is dried by nitrogen; and (4) redissolution is conducted, analysis and detection are conducted by the high performance liquid chromatography detection method. The high performanceliquid chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the human plasma has the advantages that accuracy is high, reproducibility is high, sensitivityis high, specificity is high, the efficiency is high, flux is high, the cost is low, and the operation is easy, convenient and fast.

The invention relates to a liver cancer tumour marker AFP, AFP variant and GP73 antigen synchronous immunodetection kit, a method and application, and belongs to the technical field of immunodetection. The kit comprises magnetic particles, AFP and GP73 antibody coupled mixed acridinium ester, GP73, an AFP calibration product, first stimulation solution, second stimulation solution, washing solution and all kinds of diluting solution; and the magnetic particles are respectively marked by AFP, LCA-BSA and GP73. By adoption of the kit and the detection method, the sensitivity, the specificity andthe accuracy are high; the detection time is short; identification on benign liver diseases and malignant liver diseases and early diagnosis on liver cancers can be easily carried out; and the kit and the detection method have an important purpose for increasing the early diagnosis rate of patients with liver cancers and prolonging the survival rate, and have wide clinical application value.

The invention belongs to the technical field of biology, and relates to an improved high performance liquid chromatography-tandem mass spectrometry detection method for 15 bile acids in human serum. The method comprises the steps of protein precipitation, sample dilution and analysis and detection by high performance liquid chromatography-tandem mass spectrometry. The method has the advantages ofhigh efficiency, low cost, simple operation, high accuracy and sensitivity and the like, and is suitable for rapid detection of a large number of clinical samples.

The invention relates to a usage of a replication-defective recombinant adenovirus which contains the expression sequence of human APE1 gene siRNA, and the invention can be used in the preparation of the drugs to play sensitizing effect in the radiotherapy and / or chemotherapy of malignant tumors. The expression sequence of human APE1 gene siRNA is inserted below the U6 promoter of adenovirus shuttle plasmid, so as to construct shuttle plasmid pDC316-EGFP-U6-APE1siRNA, and the latter and adenovirus backbone plasmid pBHG-fiber5 / 35 transfect 293 cells in total, and the Ad5 / F35-APE1siRNA recombinant adenovirus containing the expression sequence of human APE1 gene siRNA is obtained by fixed-point recombinant by a Cre / loxP system. The recombinant adenovirus can be made into the injection liquid with certain titer, be used in the combined application in the radiotherapy and / or chemotherapy of malignant tumors, effectively control the expression of APE1 gene and effectively enhance the sensitivity to the radiotherapy and / or chemotherapy of malignant tumors.

The invention discloses a compound Chinese traditional medicine preparation for inducing immune tolerance and preparing method and application thereof. The compound Chinese traditional medicine preparation comprises 2-30 parts of roots of Chinese thorowax, 3-31 parts of moutan bark, 4-32 parts of radix paeoniae alba, 5-33 parts of radix curcumae and 6-35 parts of liquorice. The above components are prepared according to the proportion, to be smashed, soaked with water, decocted, filtered, centrifuged and dried to be made into capsules, oral liquid, tablets and the like. The compound Chinese traditional medicine preparation can induce generation of DC regs of high-expression CD11b and low-expression HLA-DR / CD86 / CD80, the DCregs generated through inducing can effectively inhibit DC-mediated lymphopoiesis and promote secretion of cytokines IL-10; a receptor mice is induced to tolerate the iso-allotransplantation skin; no influence on the motility rate of DC cells and the hepatorenal function of the receptor mice is generated, and the compound Chinese traditional medicine preparation is effective and low-toxic and induces immune tolerance.

The present invention discloses an anti-tumor polypeptide and an application thereof in preparing anti-tumor drugs. Aiming at a molecular structure, a ligand binding mode and amino acid sequence characteristics of a NRP-1 transmembrane region, and the transmembrane region capable of specifically binding the human source NRP-1 molecular structure and binding ligands is designed to block binding ofNRP-1 with a variety of the ligands of VEGF, EGF, and HGF, thereby blocking the anti-tumor polypeptide drugs of downstream cellular signaling pathways. The anti-tumor polypeptide binds to the NRP-1 transmembrane region monomer competitively, interferes dimerization of NRP-1, inhibits the binding of the NRP-1 to the ligands, thus inhibits activation of various tumor cell signaling pathways, and achieves purposes of inhibiting tumor cell proliferation, migration and infiltration, and promoting apoptosis. The provided anti-tumor polypeptide provides a new technical means for treatment of tumors.

The invention relates to the technical field of medicines, in particular to Chinese medicine preparations for treating gastric malignant tumors. The invention adopts a traditional Chinese medicine which comprises the following ingredients: 0g to 6g of sesames, 0g to 6g of cicada skins, 10g to 15g of radix codonpis pilosulae, 6g to 8g of morindae radix officinalis, 10g to 6g of baicalin, 6g to 12g of sweet potato, 20g to 30g of dioscoreae rhizome, 0g to 6g of millet, 10g to 15g of sparrow meat, 0g to 6g of myrrh, 0g to 6g of peppermint, which are configured to preparations according to certain content and are comminuted to produce powders for refining the sweet into pills which are taken by warm water in post cibum three hours., 7 days for one course and 3 courses for tumor alleviation. The invention has a clinical value with wide application.

The invention discloses an anti-tumor polypeptide with double effects and an application thereof. The anti-tumor polypeptide with double effects has an amino acid sequence shown as SEQ ID NO.4. The polypeptide provided by the invention can specifically target NRP-1 molecules expressed on endothelial cells and tumor cells, improve the permeability of vascular endothelial cells and increase the permeability of tumor tissues, thereby improving the capacity of a drug penetrating through a vascular barrier and permeating into the tumor tissues. Meanwhile, proliferation of cancer cells can be inhibited and apoptosis of the cancer cells can be promoted by blocking a downstream cell signal path regulated and controlled by NRP-1. The polypeptide disclosed by the invention can be used for treating various solid tumors including pancreatic cancer, and can improve the effect of treating malignant tumors by using drugs, especially using a monoclonal antibody targeted drug.

The invention discloses an orthoester miscible pharmaceutical adjuvant, which relates to the field of pharmaceutical adjuvants and is mainly obtained by mixing and dissolving different orthoester compounds according to different proportions or mixing and dissolving orthoester compounds and biocompatible medical polymer materials according to different proportions. The chemical formula I of the orthoester compound is shown in the specification, wherein R represents hydrogen, methyl, ethyl, propyl, isopropyl, butyl or phenyl. The invention also provides a preparation method of the pharmaceutic adjuvant and a local sustained-release drug delivery preparation containing the adjuvant. The invention has the following beneficial effects: the fluidity of the orthoester miscible pharmaceutical adjuvant is adjustable; the degradation rate is adjustable, and the drug release rate is adjustable; a good solvent, which can dissolve small molecule and protein drugs; local injections, cream and ointment can be prepared; the biological compatibility is good, the metabolism is clear, and the clinical conversion use is easy.

The present invention belongs to the field of Chinese medicine technology, and is especially one kind of depression treating Chinese medicine preparation, which is honeyed bolus prepared with 11 kinds of Chinese medicinal materials, including white tuckahoe, cicada slough, lotus seed, skullcap root white peony root, etc in certain weight proportion. It possesses wide clinical application value.

The invention belongs to the technical fields of stem cells and organic chemistry, and discloses a drug for promoting the function recovery of rat spinal cord injuries, and an animal model thereof. Different doses of methylprednisolone, utilization of anti-inflammatory and anti-apoptotic neuroprotective effects and transplantation of amnion derived neural stem cells at different times after operation are combined, and whether the combination of the drug and the animal model can promote axonal regeneration and function recovery after experiment rat spinal cord injuries or not, so the drug explores a new therapeutic strategy for SCI repairing, and has great theoretical significance and broad clinical application prospect. Cell transplantation is combined; the neuroprotective effect of methylprednisolone and the nerve repairing effect of HAM-NSCs are combined to explore a new strategy for SCI treatment; the drug provides a theoretical basis for exploring the recovery mechanism of nerve injuries, and provides a feasible scheme for clinical treatment of the spinal cord injuries; and the drug effectively reduces the disability rate of SCI, improves the living quality of patients with thespinal cord injuries, and has broad clinical application values.

The invention belongs to the field of biological medicines, and particularly relates to application of iron death inhibition in preparation of a medicine for preventing lung injury caused by drowning.The iron death inhibitor is Ferrostatin-1. Mouse and cell experiments verify that the ferro-death inhibitor Ferrostatin-1 inhibits ferro-death, obviously improves mouse lung injury and cell injury caused by drowning, and a remarkable protective effect on lung injury caused by drowning is achieved. The invention provides a new embedding point for preventing and treating lung injury caused by drowning.

The invention discloses a traditional Chinese medicine preparation for curing scapulohumeral periarthritis and a preparation method thereof. The traditional Chinese medicine preparation is prepared through raw medical materials including turmeric, radix clematidis, radix toddalae, frankincense, myrrh and borneol and comprises turmeric extract, an essential oil component, total saponins extract, a polysaccharide active constituent and a borneol active constituent. Extraction is carried out to the turmeric in an alcohol and water combining method, first the essential oil component is collected, and then the turmeric extract is obtained by refining in a macroporous resin purification method. First the essential oil component is collected from the radix clematidis, the radix toddalae, the frankincense and the myrrh, and then the total saponins extract is obtained by using a macroporous adsorption resin column to purify extracted solution. The polysaccharide active constituent is obtained by adopting a hollow fiber column to carry out ultrafiltration on sediments of the radix clematidis, the radix toddalae, the frankincense and the myrrh. Traditional Chinese medicines and an advanced preparation process are combined, the bioavailability of the obtained preparation is high, and the medicine effect is remarkable.

A method and instrument for detecting and analyzing the predication degree index of cardiacrhythm variability features that the predication degree is used as the index of detecting and analyzing cardiac rhythm variability. Said method includes such steps as using dynamic electrocardiograph to record cardioelectric signals, automatically recognizing dynamic cardioelectric RR gap, forming time sequence RRi, and calculating non-linear predication degree PI.

The invention discloses a polypeptide capable of treating Alzheimer's disease and a vaccine containing the polypeptide capable of treating the Alzheimer's disease. The polypeptide drug has an amino acid sequence selected from any one selected from the amino acid sequences shown in SEQ ID NO. 1 to SEQ ID No. 10. The polypeptide drug is capable of prolonging survival periods of mice with the Alzheimer's disease; and thus, the polypeptide drug disclosed by the invention can be used as a drug candidate for treating the Alzheimer's disease. Thus, the polypeptide drug has extensive clinical application values.