23 results about "Surgical resection" patented technology

Indivi dualized neoantigen vaccines, as well as methods for their use in subjects to treat urothelial orbladder cancer are provided. In some embodiments, individualized neoantigen vaccines are used with an immune checkpoint inhibitor and optionally enfortumab vedotin to treat a urothelial or bladder cancer after surgical resection. Prior to surgical resection, in some embodiments, the subject is administered a neoadjuvant therapy comprising an immune checkpoint inhibitor, individualized neoantigen vaccines, and, optionally, enfortumab vedotin.

This disclosure provides methods for treating tumors, reducing tumor severity, inhibiting tumor growth, or inducing tumor necrosis, wherein the methods include: selecting a patient with cancer (e.g., liver cancer, lung cancer, or head and neck cancer) in need of treatment and administering neoadjuvant therapy to the patient in a combination of a therapeutically effective amount of a programmed death 1 (PD-1) inhibitor (e.g., cimiprimab or its bioequivalent) and radiation therapy (e.g., SBRT) or an anti-LAG-3 antibody (e.g., fenpromab), followed by surgical resection and optionally administration of a programmed death 1 (PD-1) inhibitor (e.g., cimiprimab or its bioequivalent) as postoperative adjuvant therapy. In some embodiments, the cancer is liver cancer, such as hepatocellular carcinoma (HCC).

We disclose a composition, comprising a compound, comprising a melanin precursor that spontaneously synthesizes a melanin when in free form in vivo, and a peptide directly covalently bonded to or indirectly linked to the melanin precursor, wherein the direct covalent bond or indirect link of the peptide to the melanin precursor blocks spontaneous synthesis of the melanin in vivo in the absence of protease activity. We also disclose methods of tumor imaging, tumor detection, diagnosis, and treatment, including methods comprising administering, to a patient suffering from a tumor, the composition; and either surgically resecting the tumor or thermally ablating the tumor, wherein the surgical resection is guided at least in part by contrast imparted by, or the thermally ablating targets cells containing, the melanin, wherein the melanin is spontaneously synthesized in the tumor after administering the composition.

According to the radioactive hydrogel and the preparation method and application thereof and the radioactive tumor treatment application, chitosan serves as a matrix and is crosslinked with genipin to construct a three-dimensional network, and stable loading and slow release of radionuclides can be synchronously achieved; the self-antibacterial and adhesion characteristics of the patch can reduce the infection risk and ensure the fixation of the patch, so that a safe, effective and easy-to-operate local treatment platform is provided, the prepared patch can be directly placed on a tumor bed after resection in an operation, beta or alpha rays released by radionuclides accurately and continuously act on an area where tumor cells are most likely to remain, and the effect of treating the tumor cells is achieved. Different from traditional external radiation radiotherapy, the hydrogel can continuously release radiation with a low dosage rate. The mode has a stronger biological effect (such as inhibition of reproliferation) on tumor cells, the radiosensitivity difference of different cell periods and time phases can be overcome, the killing effect is more thorough, and chitosan is proved to have certain antibacterial activity, tumor cell growth inhibition activity and immunomodulatory activity. A synergistic effect can be generated with radionuclide, and a microenvironment which is not beneficial to survival and relapse of tumor cells is constructed together with the radionuclide.

A method, system, medium, and implementations for computer-aided preoperative surgical planning are described. Input data acquired with respect to a part of a patient is received by the system. The part corresponds to an organ, e.g., lung, of the patient to be operated on and includes one or more lesions to be removed during an operation. Then, an anatomic 3D model of the part of the patient is generated. Based on the generated anatomic 3D model, a preoperative plan for linear-cutting stapler resection of the one or more lesions from the organ to be carried out during the operation is obtained. The stapler cartridge size and the staple length are estimated based on the preoperative plan. Further, the resection based on the preoperative plan is visualized.

The invention discloses a method for constructing a liver injury animal model, which comprises the following steps: (a) providing a model animal, and completely anesthetizing the model animal; (b) the liver of the model animal is taken out, and the surgical resection proportion is determined; and (c) fixing the liver part needing to be cut by using a surgical suture line, and performing liver cutting. According to the method disclosed by the invention, the liver injury mouse model can be more efficiently constructed, and liver injury proportions required by different liver injury models can be met.

Methods of producing an incised tissue are presented. The methods may include: contacting a portion of a tissue grasping implement of a first robotic manipulator with a tissue to produce contacted tissue, grasping the contacted tissue using the tissue grasping implement of the first robotic manipulator to produce grasped tissue, and creating an incision in the grasped tissue using a surgical cutting implement of a second robotic manipulator to produce the incised tissue. Additional methods as well as related systems and computer readable media are also presented.

w The invention discloses a detection method for predicting efficacy and prognosis of immunotherapy for small cell lung cancer based on ATAD2 expression, and relates to the technical fields of molecular biology and cancer treatment. The method comprises the following steps: extracting a tumor tissue sample of a small cell lung cancer patient through surgical resection; Detecting the tumor tissue sample to obtain ATAD2 expression level, wherein the detection adopts an immunohistochemical staining method. According to the invention, the expression level of ATAD2 in tumor tissues of small cell lung cancer is quantitatively evaluated by immunohistochemical detection technology, and the molecular marker information is systematically correlated with the clinical and pathological characteristics of patients by combining Cox regression analysis and Logistic regression analysis, so that the comprehensive prediction of immunotherapy efficacy and patient survival time is realized.

The invention belongs to the technical field of immune cell culture, and particularly relates to a tumor infiltration lymphocyte amplification composition, a kit and an amplification method thereof. The invention provides the tumor infiltration lymphocyte amplification composition aiming at a trace amount of tumor tissue, namely a tumor puncture sample, the tumor infiltration lymphocyte amplification composition is used for amplifying the tumor infiltration lymphocyte, the problem that the tumor tissue sample size required by the tumor infiltration lymphocyte is large is solved, and the tumor infiltration lymphocyte amplification composition is suitable for a patient who cannot receive the tumor tissue obtained by surgical excision. Besides, by optimizing the antibody stimulant combination and the working concentration and establishing the technological process for amplifying the tumor infiltration lymphocytes based on the puncture sample, the dryness of the tumor infiltration lymphocytes obtained through amplification is maintained, the lethality is improved, and therefore the anti-tumor curative effect after the tumor infiltration lymphocytes are infused back into the body is guaranteed.

The invention discloses a glioma operation boundary identification marker and application thereof. The invention confirms that SERPINA3 can be used as a marker for identifying a glioma invasion boundary for the first time, can effectively assist operation planning and tumor excision before or during glioma operation, improves the accuracy of operation excision, provides an effective molecular tool for clinical diagnosis and treatment of glioma, and has a remarkable application prospect.

The application discloses a brain network analysis method based on step-by-step information flow and de-redundancy, and belongs to the field of image analysis; solves the problems that a simple network building mode cannot reflect the information flow propagation process between specific regions at different levels, and information redundancy caused by the repeated existence of some edges in different high-order adjacency matrices, etc. The interaction between electrodes at the connection level is mainly captured through a step-by-step process, and the redundant information between matrices of different orders is eliminated by using a de-redundancy module. Finally, the epilepsy propagation path is successfully captured, thereby providing a reference value for surgical resection of intractable epilepsy patients.

Provided herein are compositions and methods for treating a glioblastoma in a patient after surgical resection, radiation therapy and chemotherapy comprising administering to the patient a therapeutically effective amount of an intravenous liposomal curcumin or curcuminoids to a human subject in need thereof after surgical resection of the glioblastoma a dosing regimen comprising the therapeutically effective amount of the liposomal curcumin or curcuminoids of at least 300 mg / m2 over 8 hours or less, and wherein a treatment cycle is for 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 consecutive weeks.

The invention discloses a construction method of a lung organ model based on a COPD patient source, relates to the technical field of biomedical model construction, and efficiently constructs a lung organ model retaining COPD pathological characteristics by optimizing tissue treatment, cell separation and three-dimensional culture conditions. The method comprises the following specific steps: acquiring fresh surgical resected lung tissues of a COPD patient; primary lung epithelial cells are obtained by combining collagenase IV digestion with cell filtration and lung interstitial cell separation; the method comprises the following steps: culturing cells by a matrigel embedding method, and culturing for 10 days by utilizing an amplification culture medium to form an organ-like structure; and then switching to a differential culture medium and inducing for 7 days to realize functional differentiation of the airway epithelial cells and the pulmonary alveolar cells. According to the method, the construction efficiency and stability of the organoid are remarkably improved, the constructed model can simulate the COPD pathological microenvironment, and the method is suitable for disease mechanism research, drug screening and individualized treatment evaluation and has high reproducibility and clinical transformation potential.

The invention belongs to the mass spectrum technology, and particularly provides a mass spectrum data analysis method and system based on multi-mode perception and intelligent prediction.The analysis method comprises the steps that A1, in the operation, a direct ionization mass spectrometer is used for scanning the surface of an excision cavity, and a metabolite concentration vector is obtained; performing full-field scanning to obtain optical characteristic data; collecting intraoperative image data; a2, performing spatial registration on the obtained multi-modal data, inputting mass spectrum point data into a pre-training model to obtain a pathological state probability, and taking the pathological state probability as anchor point data; a3, performing fusion model adjustment by taking the optical data and the intraoperative image as main input and taking the anchor pathological state as a training label, and outputting a full-field pathological state probability graph; and A4, according to the pathological state probability graph, dividing an excision cavity into a plurality of level areas according to a preset threshold value, overlapping and displaying information of the level areas in a surgical field, and guiding surgical excision. The invention has the advantages of accurate tumor excision and the like.

The present application relates to the technical field of tumor biology, and specifically discloses a human malignant giant cell tumor of bone cell line and application thereof, the human malignant giant cell tumor of bone cell line is preserved on April 9, 2025, the preservation address is China Center for Type Culture Collection, located in Wuhan University in Wuhan, Hubei Province, and the preservation number is CCTCC NO:C202539.The human malignant giant cell tumor of bone cell line of the present application is from the tumor tissue after surgical resection of a Chinese malignant giant cell tumor of bone patient, and is suitable as cell material for developing malignant giant cell tumor of bone related immunotherapy, diagnosis and drug development.The cell line of the present application contains H3F3A G34W mutation, and has high sensitivity and specificity in the diagnosis of malignant giant cell tumor of bone.This cell line can be used as a tool cell for researching target drugs with H3F3A as a target, provides a cell model for Chinese malignant giant cell tumor of bone research, and provides an experimental platform for mechanism research of malignant giant cell tumor of bone.

The present invention provides a multifunctional preoperative precise locator suitable for multiple organs, comprising a guiding system and a positioning system. The guiding system includes a guiding needle, a guiding needle handle, scale lines, rubber limiters, sharp needle cores, and blunt needle cores. The present invention enhances the safety and ease of operation of preoperative localization of small nodules in various organs, broadens the range of guidance devices for preoperative localization: ultrasound, CT, PET / CT, and MRI can all be used, significantly improves the accuracy of localization, can precisely guide the surgical resection range, and maximizes the preservation of residual anatomical structures and functions of organs in patients.

Individualized neoantigen vaccines, as well as methods for their use in subjects to treat non-small cell lung cancer (NSCLC) are provided. In some embodiments, individualized neoantigen vaccines are used with an immune checkpoint inhibitor to treat a resectable NSCLC tumor after surgical resection. Prior to surgical resection, in some embodiments, the subject is administered a neoadjuvant therapy comprising an immune checkpoint inhibitor and chemotherapy.

A method for marking a lesion is disclosed herein. The disclosed method can assist a surgeon in identifying the location of a nodule or other lesion by marking the nodule or other lesion using a reference marker impregnated with a luminescent material, such as a reference coil impregnated with a fluorescent dye. In some preferred embodiments, the reference coil is placed using a robotic surgical system during a biopsy. In alternative embodiments, a non-robotic peripheral navigation platform may also be effectively used as an aid to surgical resection. The dye marks the location of the nodule or other lesion, allowing it to be visualized and detected by palpation during surgery. Accordingly, the surgeon can target the tissue to be removed with greater accuracy. The disclosed method enables the reference marker to be placed several days prior to surgery.

This invention relates to the fields of bionanotechnology and molecular imaging, disclosing a probe, its preparation method, and its applications. The probe comprises, from the inside out, a near-infrared II (NIR-II) fluorescent core, a gold nanoshell, an MMP-responsive peptide layer, and a pH-sensitive polymer layer. Utilizing the quenching effect of the gold shell on the core fluorescence, the probe remains in a "silent" state in the circulatory system. Its core design lies in the physical shielding effect of the peptide layer, ensuring that the probe must first be unlocked by cleavage by MMP enzymes highly expressed in the tumor, followed by protonation and shedding of the polymer layer under a slightly acidic environment to restore fluorescence. This invention effectively overcomes the non-specific activation of single-response probes in inflammatory or necrotic areas, significantly improves the imaging contrast at the tumor invasion front, and provides a highly sensitive and deeply penetrating visual navigation method for precise surgical resection of tumors.

This invention discloses a method for constructing a mouse model of spontaneous brain tumor recurrence. The method includes: injecting a viral vector with a nucleotide sequence as shown in SEQ ID NO:1 into the mouse brain; after tumor formation, injecting AP20187; monitoring the in situ recurrence of residual cells within 14-21 days to obtain a mouse model of spontaneous brain tumor recurrence. This model is the first to simulate the entire process of glioblastoma development, including spontaneous tumor formation, simulated surgical resection, and highly invasive recurrence, with a 100% recurrence rate, providing an irreplaceable preclinical platform for screening anti-recurrence drugs.

The present invention is directed to methods for detecting a melanoma, methods for determining whether a melanoma is stable or progressive, methods for evaluating the extent of surgery resection in a subject having a melanoma, and methods for determining a response by a subject having a melanoma to a therapy.

The application discloses an individual structure brain network-based epilepsy postoperative curative effect prediction system and belongs to the technical field of biomedical image pattern recognition. The system is based on preoperative structural magnetic resonance imaging data of healthy subjects and epilepsy patients. Firstly, a standard structural covariant network model is constructed in the healthy subject group. Then, the preoperative structural data of the patients is added to the standard model one by one to construct an epilepsy individual disturbance offset network, and the network is standardized. Meanwhile, by adding virtual surgical resection information, the structural network characteristics of the reserved brain area after surgery are calculated and input into a support vector machine classification framework to establish a classification model related to the surgical curative effect. The system quantifies the individual structural image characteristics of the epilepsy patients by constructing the structural covariant network, and realizes intelligent prediction of the curative effect of epilepsy surgery by using the characteristics of the network, so that more comprehensive brain image information reference can be provided for preoperative accurate evaluation.

Malignant glioma has a poor prognosis, despite current therapeutic modalities. Standard of care therapy consists of surgical resection, fractionated radiotherapy concurrently administered with temozolomide (TMZ), a DNA-alkylating chemotherapeutic agent, followed by adjuvant cycles of TMZ. O6-methylguanine DNA methyltransferase (MGMT) is a DNA repair enzyme which removes alkylated lesions from tumor DNA, thereby promoting chemoresistance. MGMT promoter methylation is a known predictor of temozolomide responsiveness; patients with unmethylated MGMT have a poorer prognosis. We employed deactivated Cas9-CRISPR technology to effectively target methylation in the vicinity of the MGMT methylation-specific polymerase chain reaction (MSP) region, as mediated by the catalytic domain of DNA methyltransferase DNMT3A. In doing so, we discovered that the selected dCas9 / CRISPR-mediated methylation of certain regions of MGMT gene sequences can be used to enhance chemosensitivity in malignant glioma cells.