The invention relates to a unibus sensor network protocol which follows the ISO/OSI standard model and provides a physical layer, a data link layer and an application layer. The physical layer defines a substrate communication medium, a driving mode and the form of a connecting terminal. The unibus sensor network protocol is characterized in that the data link layer defines the space size of the address of a node and the addressing method, the maximal transmitting data byte number, the composition method of data frames, the verification method and the arbitration method; during the compunctions process, the data of the application layer is transmitted in a manner of being packed in the data field of the link layer, the data of the application layer include the operating codes, the channel numbers, the command codes, the parameters related to the command codes, the transmitted data, the filling data, and the length, the parameters and the data total length of the parameter data of one frame. The unibus sensor network protocol supports both the master-slave communication mode and the event/action communication mode and improve the flexibility of network organization; supports a bit-based non-destructive bit-by-bit arbitration mode; meets the real-time requirement; supports the node event, the action configuration and the channel operation of the node; reduces the burdens of the host machine; facilitates the integration of the nodes; and reduces the production cost of the product.