88results about How to "Improved Axial Resolution" patented technology

The present invention provides a method, system and apparatus for reflection-mode microscopic photoacoustic imaging using dark-field illumination that can be used to characterize a target within a tissue by focusing one or more laser pulses onto a surface of the tissue so as to penetrate the tissue and illuminate the target, receiving acoustic or pressure waves induced in the target by the one or more laser pulses using one or more ultrasonic transducers that are focused on the target and recording the received acoustic or pressure waves so that a characterization of the target can be obtained. The target characterization may include an image, a composition or a structure of the target. The one or more laser pulses are focused with an optical assembly of lenses and / or mirrors that expands and then converges the one or more laser pulses towards the focal point of the ultrasonic transducer.

The invention discloses an inclined wide-field optical section scanning imaging microscope system and an imaging method thereof. The inclined wide-field optical section scanning imaging microscope system comprises a laser transmitting device, wherein laser transmitted by the laser transmitting device enters a laser scanning optical path consisting of a two-dimensional scanning galvanometer, a collimator lens group and a first microscope objective to perform inclined scanning on a sample on a sample platform; a second microscope objective, a field lens and a detector form an imaging detection optical path the optical axis of which is perpendicular to the laser scanning optical path; an imaging display control device respectively controls the synchronized action of the two-dimensional scanning galvanometer, the second microscope objective and the detector and the automatic displacement of the sample platform through a data acquisition card and a sample platform control device, and processes acquired imaging data of the detector to form wide field three-dimensional image information of the sample. The imaging microscope system and the imaging method disclosed by the invention have high scanning imaging speed and high resolution and can implement wide field scanning imaging.

The invention relates to a method for fixing a focus and measuring a curvature radius by confocal interference, and belongs to the technical field of optical precision measurement. The method comprises the following steps of: introducing interfering reference light based on a confocal light path; precisely positioning an apex of the surface of a tested spherical element and a location of the centre of a sphere by using the maximum value of a confocal interference response curve to obtain the curvature radius of the surface of the tested spherical element; and maximumly sharpening a main lobe of the confocal response curve. By the invention, the traditional confocal interference microscopy imaging technique is first applied to improvement on the fixed-focus precision of an optical measuring system, so that the optical measuring system has a higher axial resolution and a simple structure, and the research and development costs of the system device are reduced.

The invention discloses a two-dimensional modulation technique-based method for acquiring shear-layer images. In the method, a two-dimensional light modulator, such as a digital micro lens DMD, a transmission LCD and a reflective silicon-base LCOS, instead of a fiber grating in a microscope with the conventional structure is used for two-dimensional light modulation and generating a group of modulated patterns in different phases, and the modulated patterns as well as a two-dimensional phase-shift algorithm are used to rebuild the shear-layer images. Experiments show that under a condition ofkeeping the signal-to-noise ratio of the images, the shear-layer images rebuilt by two-dimensional modulation technology have a higher axial resolution compared with that of the shear-layer images rebuilt by one-dimensional modulation technology.

An optical sensor has: a sensing portion having an optical fiber to be disposed at a measurement point of temperature, distortion, pressure etc.; a light source to output a light to the sensing portion; and a photodetector to detect a backscattered light from the sensing portion. The sensing portion has a tape sheet and the optical fiber shaped into a corrugated form with a predetermined curvature. Alternatively, the sensing portion has a polymer optical waveguide with a core shaped into a corrugated form with a predetermined curvature.

A non-invasive system and method. Sample light is delivered into an anatomical structure, such that a portion of the sample light passes through a target voxel comprising brain matter in the head and is scattered by the head as signal light. The signal light is detected, changes in the level of water concentration or relative water concentration of the target voxel are detected based on the detected signal light, and a level of neural activity is determined within the target voxel based on the determined changes level of the water concentration or relative water concentration of the target voxel.

The invention discloses a double helix electrode capacitance tomography sensor for measuring an annular space that belongs to the field of an electrical process tomography device. The sensor comprises a main structure part and a capacitance measurement part. The main structure part is composed of concentric inner and outer tubes and a connection bracket used for fixing position. An annular space between the inner and outer tubes is a measured space. The capacitance measurement part comprises detection electrodes, electrode end shields, shielding cases, a signal transmission cable, ECT signal acquisition equipment and connection wires. By the utilization of electrode sensor parts which are respectively spirally arranged on the inner and outer tube walls, three-dimensional imaging of the measured annular space is truly realized. Due to the sensor structure with electrodes uniformly distributed on the two tube walls, imaging of the annular space is realized, and the problem of weak capacitance signal is also improved. The invention is a big breakthrough of the ECT technology.

The invention discloses a wide-view-field chromatography hyperspectral microscopic imaging method and device based on space-time focusing, and belongs to the technical field of microscopic spectral imaging and analytical chemistry. According to the method, ultra-short pulse lasers are generated through an ultra-short pulse laser source, focusing lines are generated in a sample through space-time focusing, excited fluorescence is collected, stray light is filtered out through con-focal optical slits, spectral information (x, lambda), of the sample is acquired through fluorescence spectral information collecting, and finally sample (x, lambda, y, z, t) five-dimensional information is acquired through three-dimensional space scanning and delayed scanning. The device comprises the ultra-shortpulse laser source, a light beam conversion system, a line scanning system based on space-time focusing, an optical microscopic system and a filtering and synchronous spectrum con-focal detection system. Acquisition of the spectral information in the filtering and synchronous spectrum con-focal detection system is synchronous with line scanning trigger signals in the line scanning system in combination with the space-time focusing technology. The method and device have the advantages of being wide in view field, high in space resolution, high in time resolution, high in spectral resolution andthe like.

The invention discloses a method and a system for calibrating a spectrum based on interference spectrum phase information. In a sweep frequency optical coherence chromatography imaging system, a Mach Zehnder interferometer interference spectrum signal is generated by a Mach Zehnder interferometer, and an unwrapping phase of the Mach Zehnder interferometer interference spectrum signal is acquired in a computer based on digital Hilbert transform; the unwrapping phase and the wave number of a sweep frequency light source form a linear relation; equiphase interval interpolation is performed on an optical coherence chromatography signal by using unequal interval phase distribution; and optical coherence chromatography signal sampling points in equal wave number interval distribution are acquired so as to perform an image reconstruction algorithm based on fast Fourier transformation. In the invention, all the acquired sampling points are used for interpolation, so the interpolation precision is enhanced greatly, and the signal to noise ratio of the reconstructed signal is improved; actual axial resolution approximating to the ideal resolution can be obtained; moreover, the algorithm is simple, and real-time and high-sensitivity calibrated data processing and image reconstruction can be implemented.

The invention discloses a confocal microscopic imaging system based on the laser heterodyne interferometry. The confocal microscopic imaging system is characterized in that on the basis of a microscope optical system and a scanning part of the existing laser confocal microscope, a frequency-shifting part is added, and accurate measurement is realized by combination of the optical heterodyne interferometry. The confocal microscopic imaging system disclosed by the invention has the advantages that the ultrahigh lateral resolution of a confocal scanning microscope is fully utilized, and simultaneously accurate phase information is acquired for replacing intensity information, so that not only is the axial resolution increased, but also a series of problems brought by use of a fluorescent dye are avoided; and a transparent phase object can be measured under the condition without marks.

A system and method of performing ultrasound modulated optical tomography. Ultrasound is delivered into a target voxel in an anatomical structure, and sample light is delivered into the anatomical structure, whereby a portion of the sample light passing through the target voxel is scattered by the biological tissue as signal light, and a portion of the sample light not passing through the target voxel is scattered by the anatomical structure as background light. The ultrasound and sample light are pulsed in synchrony, such that only the signal light is frequency shifted by the ultrasound. Multiple pulses of the sample light are delivered into the anatomical structure for each pulse of the ultrasound delivered into the target voxel. Reference light is combined with the signal light and background light to generate an interference light pattern, which is sequentially modulated to generate different interference light patterns, which are detected.

The invention discloses a laser scanning saturate structured light illumination microscopic method based on phase modulation, and the method comprises the steps: enabling an illumination light beam after collimation to be converted into linear polarized light; loading a phase (0-phi) in a first direction to the linear polarized light, and adjusting the polarization direction of the linear polarized light; scanning a sample through the linear polarized light after the adjustment of the polarization direction, forming a first laser scanning saturate structured light illumination pattern where the sample is excited to generate fluorescent light, and collecting a first fluorescent light signal; loading a phase (0-phi) in a second direction to the linear polarized light, and adjusting the polarization direction of the linear polarized light; scanning a sample through the linear polarized light after the adjustment of the polarization direction, forming a second laser scanning saturate structured light illumination pattern where the sample is excited to generate fluorescent light, and collecting a second fluorescent light signal; carrying out the processing of the first and second fluorescent light signals, and obtaining a super-resolution image with the improved lateral resolution. The invention also discloses a laser scanning saturate structured light illumination microscopic device based on phase modulation.

The invention discloses a spectrally encoded confocal and optical coherence tomography cooperative imaging method and system. The system is based on the combination of an OCT (Optical Coherence Tomography) imaging technique and an SECM (Spectrally Encoded Confocal Microscopy) technique, a sample arm of an OCT system and a sample arm of an SECM system share the same light path, so that light beams emitted from the sample arms of the OCT system and the SECM system simultaneously scan the same position of a sample, and light which is scattered and returns back from the sample at a single time is respectively transmitted to a detection arm through respective light paths. The detection arm adopts two optical fiber collimating lenses to irradiate an optical grating from different incident angles, so that the optical grating diffracts two groups of spectral signals which are emitted without crossing over, i.e. SECM and OCT signals. The light path of the sample arm in the system is simpler, the implementation is facilitated, the light path is more compact and stable, the system imaging quality is high and biological tissue structures can be imaged more accurately.

The invention provides a method and a device of fluorescence microscopy by using a pyramid lens to generate structured lighting. The device comprises parallel beams, a structured lighting system, a sample cell and an image acquisition system, wherein the structured lighting system comprises a pyramid lens, a telescope system and a phase-shifting glass sheet; and the sample cell is arranged at the rear part of the phase-shifting glass sheet. The parallel beams are refracted after passing through the pyramid lens, thus generating a four-beam interference field with two-dimensional space structure distribution and exciting a sample by the four-beam interference field. The three-dimensional tomography can be realized through image processing algorithm by translating the interference optical field acted on the sample Compared with the existing microscopy for structured lighting, the invention can achieve higher axial resolution but lower photo-bleaching effect. Therefore, the invention is suitable for the research on the imaging of living creatures.

The invention discloses a high-speed large-view-field digital scanning light sheet microscopic imaging system. The system comprises a light source module, a transverse view field expansion module, a scanning module, an illumination module and a detection module which are sequentially arranged according to a light path, light generated by the light source module passes through the transverse view field expansion module to obtain an effective view field for expanding a final illumination area. The scanning module scans a line beam received from the transverse view field expansion module into a surface beam, the illumination module performs fluorescence excitation by adopting a beam splitter, a first illumination objective lens and a second illumination objective lens, and the detection module realizes fluorescence detection imaging by adopting a line detector. The axial resolution is improved, the influence of phototoxicity and photobleaching on a sample is reduced, the imaging has higher resolution and contrast ratio by using the linear array detector, the imaging speed is higher, and the cost of the detector is saved.

The invention discloses a light slice fluorescence microscopic imaging method and device based on relocation, and belongs to the technical field of optical imaging, the light slice fluorescence microscopic imaging device comprises a laser, a cylindrical mirror, a sample stage carrying a fluorescent sample, and a detection system collecting fluorescence emitted by the fluorescent sample, the laser,the cylindrical mirror and the sample stage arranged along the optical path in turn, the detection system includes a first detector, a beam splitter, a second detector, and a third detector, the detection system also includes a processor coupled to the detection system and the sample stage, the processor controls the sample stage to move along Z-axis in a fixed step length, compares a fluorescence image I1 and a fluorescence image I2 to obtain position information of each part of the fluorescence on the Z-axis, and the fluorescence information in a fluorescence image I0 is repositioned and three-dimensionally reconstructed according to the position information to obtain a three-dimensional imaging result of the fluorescent sample. The axial resolution of imaging can be improved without reducing the range of an imaging view field, increasing photobleaching of the sample, and reducing of imaging speed.

The present invention relates to a lighting device and a method, particularly relating to a lighting device for improving axial resolution of a confocal microscope imaging system. The lighting device mainly comprises a collimating lens, an annular lens with a long focal length, an annular lens with a short focal length, and a converging lens whose one focal point is in a confocal imaging system lighting needle hole, wherein the focal points of the two annular lenses coincide. According to the device, the lighting light from the light source is shaped into annular parallel beams, after convergence, the beams illuminate a sample through the lighting needle hole of the confocal imaging system and an object lens. Compared with a traditional lighting mode, according to the device, the axial illumination to an object lens non-focal plane sample is reduced, the reflection light or emitted fluorescence from the non-focal plane sample collected by the confocal imaging system is reduced, and thus the noise of an image is reduced and the signal to noise ratio of the image is raised. Thus, the axial resolution of the confocal microscope imaging system can be raised by the lighting device.

An ultrasound diagnosis apparatus having a transmission circuit which generates a transmission signal is provided. The transmission signal corresponds to a combined waveform of a trapezoidal waveform and an impulse-shaped waveform (impulse portion) . In an example transmission signal, a front slope portion, a flat portion, and a rear slope portion exist in a positive polarity side. The impulse portion has a shape which protrudes from an offset level over a base line into an opposite polarity side. Because the center frequency of the trapezoidal waveform is near the DC component, the trapezoidal waveform can substantially be ignored. The impulse portion has a large amplitude, but because the impulse portion exists over both polarities, there is no need to apply a special high voltage countermeasure for each polarity in designing the transmission circuit. A trapezoidal waveform of an opposite polarity may be added in front of the trapezoidal waveform.

The invention belongs to the technical field of chemical substance detection and relates to a differential confocal discrete fluorescence spectrum and fluorescence lifetime detection method and device. The basic thought is that a differential confocal object surface positioning technology with a precision axial resolution ratio and a discrete fluorescence spectrum and fluorescence lifetime measurement technology are fused; high-precision measurement of a three-dimensional shape of the surface of a sample to be detected is solved by utilizing a differential confocal technology; meanwhile, high-sensitivity detection of a fluorescence spectrum and fluorescence lifetime of each point on the surface of the sample to be detected is solved by utilizing the discrete fluorescence spectrum and fluorescence lifetime measurement technology, so that three-dimensional high-resolution space substance component distribution information is obtained. According to the differential confocal discrete fluorescence spectrum and fluorescence lifetime detection method and device, a differential confocal measurement technology and a fluorescence substance component detection technology are fused for the first time; a fluorescence imaging system has the same transverse resolution ratio on each position of the surface of the sample to be detected; finally, measured fluorescence spectrum distribution accurately corresponds to the three-dimensional shape. The technology provided by the invention has a wide application prospect in the fields of biological science, medical science, material science and clinical medical diagnosis.

The invention discloses an opto-acoustic / ultrasonic bimodal high-frequency probe based on ellipsoidal curvature, which comprises a probe shell, an ellipsoidal backing layer, a high-frequency piezoelectric element and a signal transmission line, a hole is dug in the middle of the side wall of the probe shell and is provided with the signal transmission line, the ellipsoidal backing layer is coaxially fixed on an incident light channel of the probe shell, a cylindrical structure is arranged in the middle of the ellipsoidal backing layer, the high-frequency piezoelectric element is pressed at the lower end of the ellipsoidal backing layer to form an ellipsoidal concave surface for expanding the depth of field, the middle part of the high-frequency piezoelectric element is provided with a middle digging hole matched with the cylindrical structure, and the shape of the ellipsoidal concave surface is matched with that of the ellipsoidal backing layer. The defect that the depth of field of an existing high-frequency probe is insufficient is overcome, the transverse resolution ratio and the axial resolution ratio of photoacoustic / ultrasonic imaging can be improved through the high-frequency piezoelectric element, and signals from deeper tissue can be received under the condition that the focus position does not need to be moved.

The present invention discloses one kind of photon type metaphenylene substituted radical perfluoro cyclopentanyl diaryl alkene photochromatic material and its preparation process and use. The photochromatic material has photochromatic performance well maintained in solution and film, excellent chemical and heat stability in both ring-opening state and ring-closing state, obvious fatigue resistance, relatively high cyclization quantum efficiency, high sensitivity and other excellent performance. It has also double photon absorbing characteristic and may be used in double photon storing record with 800 nm laser and used mainly in ultrahigh density double photon information storing.

The invention relates to a method and a device for generating a compound ultrasonic blood flow image. The method includes: setting ultrasonic pulse emitting patterns according to a distance from an interested area to skin; when two or more emitting patterns are available, generating an ultrasonic image containing blood flow information for each emitting pattern; setting a weighting function for each ultrasonic image containing the blood flow information; using each weighting function to weight the corresponding ultrasonic image containing the corresponding blood flow information; superimposing the weighted ultrasonic images to generate the compound ultrasonic blood flow image.

The invention discloses a TV constraint based tomography phase microscope reconstruction method. The method includes (1) acquiring a multi-angle phase image, (2) establishing a system matrix imaging model, (3) adding TV constraint, and (4) reconstructing a three-dimensional refractive index image. By establishing the system matrix model of microscope imaging, various uncertain factors in the data collection process are brought into a unified description framework; based on the image sparse feature, the image axial stretching effect due to missing cone is effectively alleviated, and the axial resolution of a microscope is improved; and meanwhile, the phase data is acquired by sparse angles, the total acquisition angle quantity is reduced, the imaging time is reduced, the time resolution of the microscope is improved, and the observation of the dynamic characteristic of biological samples is facilitated.

The invention discloses a transurethral ultrasound prostate detection method, a diagnostic apparatus and a transducer; the method comprises steps of transmitting an intra-prostate ultrasound transducer which is 10-100MHz in central frequency to a to-be-detected prostate part by virtue of an ultrasound catheter which is 0.5-5mm in diameter through urethra; transmitting and receiving ultrasound signals within 360 degrees to the to-be-detected prostate part; and meanwhile, retracting the intra-prostate ultrasound transducer. The diagnosis apparatus comprises an ultrasound catheter which is 0.5-5mm in diameter, wherein the front end of the ultrasound catheter is provided with an intra-prostate ultrasound transducer which is 10-100MHz in central frequency and the back end is connected to a retracting / driving device; and the retracting / driving device is connected to an electronic imaging system. The transducer comprises an ultrasound transduction unit which is formed by closely connecting a backing layer, a piezoelectric layer and an acoustic matching layer sequentially. According to the invention, the ultrasound transducer is transmitted to the prostate part through urethra, a detection range is shortened and a working frequency is improved, so that an imaging resolution ratio is increased.

Methods are proposed to improve axial resolution in optical coherence tomography (OCT). In one aspect, the method comprises: obtaining a k-space interferogram of an OCT spectral image; uniformly reshaping the k-space interferogram to a quasi-stationary interferogram by extracting a source envelope; fitting a spectral estimation model to the quasi-stationary interferogram; and calculating an axial depth profile using the fitted spectral estimation model.

The invention discloses an ultrasonic transducer, an acoustic impedance matching layer and a preparation method of the acoustic impedance matching layer. The acoustic impedance matching layer comprises magnetic particles coated with a low-density material layer and an adhesive, wherein the magnetic particles coated with the low-density material are in graded distribution in the adhesive based on different acoustic impedance, such that the acoustic impedance of the acoustic impedance matching layer are in gradient distribution from one end to the other end. The preparation method of the acoustic impedance matching layer is appropriate in difficulty. Operation equipment is simple. The prepared acoustic impedance matching layer is in uniform gradient variation of acoustic impedance, has an accurately controllable thickness, can improve the transmission efficiency of acoustic waves in the ultrasonic transducer and improves the sensitivity and the bandwidth of the ultrasonic transducer.

The invention provides a multi-mode imaging system and method based on photoacoustic and optical coherence tomography. The multi-mode imaging system based on the photoacoustic and optical coherence tomography technology comprises an OCT imaging device, a photoacoustic imaging device, a sample placing device and a control and signal processing device. The OCT imaging device comprises a first light source, a first light transmission device and a first light signal detection device; the photoacoustic imaging device comprises a second light source, a second light transmission device, a photoacoustic sensing device and a second light signal detection device; and the photoacoustic sensing device is based on the surface plasma resonance sensing technology and comprises a third light source, a first light polarization device, a prism, a photoacoustic wave coupling solution, a concave surface acoustic wave reflector and a second light polarization device. According to the multi-mode imaging system, photoacoustic imaging and OCT imaging are effectively fused, 'label-free, multi-view and self-registration 'observation of tissues is achieved, and an innovative technical means is provided for exploring a complex physiological and pathological mechanism of an organism.

The invention discloses a multi-angle based selective light-sheet illumination microscopy imaging reconstruction method, and relates to the technical field of light-sheet illumination microscopy imaging. In order to eliminate influences imposed on the image quality by strip artifacts, scattering fuzz, uneven gray and transverse and axial resolution difference in a selective plane illumination microscopy (SPIM) imaging technology, the method proposes that scale-invariant features are extracted in allusion to microscopic body data acquired at different angles, matching is performed, a rotation center is solved through a clustering algorithm, and then reconstruction is performed by using a nearest neighbor algorithm and weighted averaging so as to clear body data of a sample. According to the invention, multi-angle images acquired by a selective light-sheet illumination microscopy imaging system are fused, image noises and scattering fuzz are effectively suppressed, and the longitudinal resolution of microscopic images is improved.