8results about How to "Improved Axial Resolution" patented technology

The invention relates to the field of optical detection, in particular to an interference confocal measurement system based on two-dimensional dispersion and a measurement method thereof. White light generated by a light source is collimated into parallel light through a first collimating lens, is shaped into rectangular light through a rectangular diaphragm, and is shaped into a linear light beam through a column focusing lens and a rectangular slit; the light is divided into reference light and object light through a beam splitter, the reference light is achromatic through a first achromatic objective lens, then enters a reference plane mirror and is reflected back to the beam splitter by the reference plane mirror, the object light is focused to positions of different depths of an object to be measured after being dispersed through a dispersion lens and is reflected back to the beam splitter by the object to be measured, and the two beams of light are reconverged in the beam splitter. And after being collimated by the second collimating lens, dispersed by the dispersion grating and achromatic by the second achromatic objective lens in sequence, the light is focused on the target surface of the spectrograph for spectrum and interference analysis. According to the invention, line scanning, dispersion and interference measurement are combined, so that the measurement precision can be greatly improved.

The invention belongs to the related technical field of imaging technology, and discloses a multi-view-angle-based photoacoustic microscopic imaging system, which comprises a two-dimensional scanning module, a view angle selection module, a signal detection module and a three-dimensional reconstruction module, and is characterized in that the two-dimensional scanning module enables a light beam to carry out multiple XY plane two-dimensional scanning on a sample; the visual angle selection module adjusts the visual angle of each two-dimensional scanning to enable the angle of each two-dimensional scanning to be different, the visual angle is the direction in which the light beam enters the sample, and the visual angle of each two-dimensional scanning is perpendicular to the signal detection direction; the signal detection module is located on the side face of the sample and receives ultrasonic signals in the detection direction, the detection direction is parallel to the XY plane, and the ultrasonic signals are ultrasonic signals generated by the sample under light beam excitation; and the three-dimensional reconstruction module performs three-dimensional reconstruction according to the ultrasonic signals at different visual angles to obtain a three-dimensional body structure of the sample. According to the system, the axial resolution of photoacoustic microscopy can be improved in a simpler mode, and scanning and three-dimensional reconstruction can be rapidly completed.

The present invention provides an ultra-high resolution fluorescence microscopy imaging method based on a subvoxel convolutional neural network. An ultra-high resolution three-dimensional optical microscopy imaging model is constructed and trained based on an end-to-end three-dimensional subvoxel convolutional neural network that combines a multi-branch structure and residual learning. The original optical low-resolution image is mapped to a positioning image of a three-dimensional ultra-high resolution fluorescent probe through the imaging model, thereby being applied to three-dimensional ultra-high resolution fluorescence microscopy imaging. Compared with existing microscopic imaging technologies, the ultra-high resolution fluorescence microscopy imaging method based on a subvoxel convolutional neural network significantly improves the axial resolution of ultra-high resolution fluorescence microscopy imaging and reduces the computational complexity of three-dimensional fluorescence microscopy super-resolution reconstruction. Moreover, the ultra-high resolution three-dimensional optical microscopy imaging model successfully obtained through network training does not require additional manual parameter adjustment or additional manual intervention, and is suitable for fast, flexible, three-dimensional ultra-high resolution fluorescence microscopy imaging.

The application belongs to the technical field of optical precision measurement, and discloses a sample height acquisition method and system based on line spectrum confocal profilometry. The method comprises the following steps: S1, collecting multiple gray images of the surface profile of a sample to be measured, calculating the similarity between the gray images of adjacent sampling times and dividing the gray images into multiple groups; S2, performing Gaussian blur mean value processing and Gaussian cut-off on each group of images to obtain Gaussian-processed images, and then performing preprocessing on the Gaussian-processed images to obtain the position coordinates of the pixel points corresponding to the maximum gray value; S3, acquiring the sample height corresponding to the maximum gray value, and obtaining the sample height profile corresponding to the group of images from the sample heights corresponding to all columns; and S4, repeating steps S2 and S3 to obtain the sample height profiles corresponding to all groups of images, thereby obtaining the sample height profiles of the surface profile of the sample to be measured at different positions. Through the application, the problem of low sample height detection precision is solved.

The invention provides a differential confocal measurement system based on heterodyne illumination and a measurement method thereof, and belongs to the technical field of optical detection. The invention discloses a differential confocal measurement system based on heterodyne illumination. A heterodyne illumination unit is used for providing a pre-focus point light source and a post-focus point light source with different frequencies; the differential confocal unit is arranged in the emergent direction of the light beam of the heterodyne illumination unit and is used for receiving a pre-focus light intensity signal and a post-focus light intensity signal of the light beam reflected by the measured object and acquiring height information of the measured object; the angle measuring unit is arranged in the light beam emitting direction of the heterodyne lighting unit and used for receiving a light spot signal formed by the reflected light beam of the measured object and obtaining inclination angle information of the measured object. According to the technical scheme, the axial resolution and the signal-to-noise ratio of the differential confocal measurement system can be effectively improved, the point spread function of the differential confocal measurement system is clearer, meanwhile, the angle measurement unit for measuring the angle is added, the inclination angle of the measured object can be measured in real time, and the measurement precision is improved.

The present application relates to the field of optical microscopic imaging technology, and provides a three-dimensional differential phase contrast refractive index tomographic reconstruction method, system, device and program, the method comprising: collecting multi-mode intensity data; the multi-mode intensity comprises a three-dimensional intensity image stack and a focus and defocus angle change intensity set; determining a transfer function according to the three-dimensional intensity image stack, and obtaining an initial scattering potential spectrum by using a deconvolution algorithm; based on the focus and defocus angle change intensity set, performing a loop single LED intensity constraint update on the initial scattering potential spectrum to obtain a scattering potential estimate; using the three-dimensional intensity image stack, performing a linear gradient constraint update on the scattering potential estimate, and executing the linear gradient constraint update cyclically until a convergence condition is met, performing a three-dimensional inverse Fourier transform according to the final obtained scattering potential spectrum, and outputting a three-dimensional refractive index distribution result. The present application substantially improves the accuracy and quality of three-dimensional refractive index reconstruction while keeping the advantages of non-interference and label-free imaging and the simplicity of system hardware.

The invention discloses an ultrahigh-frequency ultrasonic transducer capable of generating quasi-unipolar pulses, and belongs to the technical field of ultrasonic transducers. The transducer comprises a backing layer, a high-frequency piezoelectric layer and a combined layer which are sequentially arranged from bottom to top. The combined layer comprises an acoustic decoupling layer, a first high-frequency matching layer, a second high-frequency matching layer, an ultrahigh-frequency piezoelectric layer, an ultrahigh-frequency polymer matching layer and an ultrahigh-frequency metal matching layer. A penetrating cavity is arranged between the first high-frequency matching layer and the second high-frequency matching layer, and an acoustic decoupling layer, an ultrahigh-frequency piezoelectric layer, an ultrahigh-frequency polymer matching layer and an ultrahigh-frequency metal matching layer are sequentially arranged in the penetrating cavity in a stacked mode. During working, the high-frequency piezoelectric layer and the ultrahigh-frequency piezoelectric layer can be driven to generate forward waves of high-frequency ultrasonic waves and ultrahigh-frequency ultrasonic waves respectively by reasonably adjusting electric excitation parameters, and the forward waves of the high-frequency ultrasonic waves and the ultrahigh-frequency ultrasonic waves are superposed, so that quasi-unipolar pulses with sharp main lobes and low side lobe levels are synthesized. The problem of pulse trailing of the ultrahigh frequency transducer is effectively solved, the axial resolution is remarkably improved, and meanwhile high detection sensitivity is kept.

The present invention discloses a near-infrared structured light illumination light sheet imaging method and device based on the fluorescence loss super-resolution method, wherein the near-infrared excitation light and the near-infrared vortex hollow loss light generated by the beam modulation module form a coaxial coupled light beam. The near-infrared excitation light can excite the sample fluorescence, and the near-infrared vortex hollow loss light can deplete the sample fluorescence in the action area, so as to realize axial super-resolution (resolution of about 100nm) imaging. The coaxial coupled light beam leads out two coherent lights through a semi-reflective and semi-mirror lens, and each of them passes through a cylindrical lens and is conjugated to the coherent light sheet formed by the rear pupils of two objective lenses to propagate in the opposite direction in the imaging plane and interfere to form a structured illumination pattern to illuminate the sample. In the imaging module, the computer performs spectrum separation and reconstruction on the collected fluorescence images, and then takes out all the images and performs three-dimensional reconstruction, finally realizing large-field three-dimensional super-resolution (resolution of about 100nm) imaging with a large imaging depth and anti-sample scattering capability.