5 results about "Fluorescence lifetime measurement" patented technology

A time measurement apparatus 10 includes a TAC circuit 12, a measurement gate 11, a control unit 14 for setting a gate dead time, which is a time during which the measurement gate 11 is set to be in the second state, in the measurement gate 11, and the control unit 14 for deriving and outputting time information related to the detection signal based on a measurement signal output from the TAC circuit 12, and the control unit 14 functioning as a setting unit sets a time, which is an integral multiple of a repetition period of fluorescence detected by the detector 4 and is longer than a dead time of the TAC circuit 12 itself, in the measurement gate 11 as a gate dead time.

This invention belongs to the technical field of fluorescence lifetime measurement, and discloses a fluorescence lifetime measurement method and system based on time-gated fluorescence imaging. The method includes: acquiring multiple frames of fluorescence images of a sample, wherein the frame rate is greater than the pulse frequency of the pulse beam, and the frame rate and pulse frequency are not integer multiples of each other; the phase difference between two fluorescence images is a delay difference between the sampling delay of the two fluorescence images and their respective reference times; the reference time of each fluorescence image is the on-time of the pulse beam corresponding to its acquisition; calculating the average gray level of the fluorescence images; arranging the average gray levels within multiple pulse beam periods according to the sampling delay to obtain a time-gated sequence of average gray level variation with sampling delay; and obtaining the fluorescence lifetime of the sample by data fitting based on the time-gated sequence. Based on this method, the complexity and cost of detection can be reduced, and the limitations on application scenarios can be lessened.

This fluorescence signal measurement method generates, by using a convolutional neural network (CNN) model on which adversarial training has been performed, a corrected fluorescence signal on the basis of a saturated fluorescence signal in which a saturation phenomenon has occurred. The fluorescence intensity of the saturated fluorescence signal in a first region is a threshold value, and the fluorescence intensity of the corrected fluorescence signal in a second region corresponding to the first region of the saturated fluorescence signal is greater than the threshold value. Fluorescence lifetime information is generated on the basis of the corrected fluorescence signal. A fluorescence lifetime image is generated on the basis of fluorescence lifetime information.

This invention provides a rapid full-spectrum fluorescence lifetime measurement device, aiming to solve the problems of slow measurement speed and difficulty in simultaneously obtaining fluorescence information at multiple wavelengths due to count rate limitations in time-correlated single-photon counting-based fluorescence lifetime measurement methods. The measurement device includes an optical system and a detection circuit system. The optical system is used to split and shape fluorescence signals of different wavelengths, separating wavelengths in one dimension and uniformly distributing light intensity in another. The detection circuit system is used to receive the shaped two-dimensionally distributed fluorescence signal, perform photon counting, obtain the time difference between the excitation light signal and the fluorescence signal, accumulate the number of fluorescence photons in each pixel unit, obtain a fluorescence lifetime decay histogram at different wavelengths, and finally obtain full-spectrum fluorescence lifetime information. This invention utilizes a time-domain-based fluorescence lifetime measurement method, combining fluorescence spot splitting and shaping with a multi-channel detector and signal processing system, to achieve rapid measurement of the full-spectrum fluorescence lifetime of a sample.

The invention discloses a fluorescence lifetime measuring device and method for a rare earth material. The device comprises an optical flat plate, an adjustable sample table, an optical fiber tail end focuser, an optical fiber tail end recoverer, a laser, a signal generator, an oscilloscope and a photoelectric detector. The device has the advantages that accurate focusing of exciting light and efficient collection of fluorescence can be achieved, and signal loss is reduced; interference of front and back excitation signals is avoided; the whole fluorescence attenuation process can be accurately captured, and the measurement error is small. The structure is designed in a modular mode, and all parts are convenient to install and adjust; the measurement method has clear steps. The output wavelength of the laser can be flexibly replaced according to the characteristic absorption band of a material to be measured, the wavelength response range of the photoelectric detector covers the characteristic fluorescence wavelength of a mainstream rare earth material, and the photoelectric detector can be adapted to measurement of rare earth materials with nanosecond-level to millisecond-level fluorescence lifetime, including various samples such as rare earth simple substances, rare earth oxides, rare earth doped fluorescent powder and the like. The measurement process does not need a complex data analysis algorithm, and is suitable for rapid detection of batch samples.