The invention is applicable to the technical field of photoelectric detection, and provides a measuring method and a measuring device of fluorescence lifetime. The measuring method comprises the following steps: firstly, using each row of a preset measurement matrix as a sampling mode, using a non-zero value position in each sampling mode as a sparse signal channel for time gate controlled delayedsampling, performing delayed sampling on a sample in each sampling mode, accumulating the number of photons in the sparse signal channel in the current sampling mode to obtain a compressed sensing measurement value, and repeating each sampling mode to obtain a series of compressed sensing measurement values; then, by virtue of the compressed sensing measurement values and through combination of the measurement matrix, solving an equation under an l1 norm optimization algorithm to obtain a sparse coefficient; finally, acting on the sparse coefficient through known reverse sparse matrix transformation, and performing signal reconstruction to obtain a fluorescence lifetime attenuation curve so as to achieve fluorescence lifetime measurement. By the measuring method provided by the invention,the measurement speed of the fluorescence lifetime is increased.