Integral femtosecond time resolution fluorescence lifetime measurement spectrograph

A time-resolved fluorescence and spectrometer technology is applied in the field of integrating femtosecond time-resolved fluorescence lifetime measurement spectrometers, which can solve the problems of reduced application and high price.

Active Publication Date: 2018-06-12
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The time resolution of the time-correlated single photon counter is at the highest tens of picoseconds; the time resolution of the streak camera can reach the picosecond level, but the price is very expensive; the time resolution of the femtosecond time-resolved fluorescence up-conversion spectrometer is limited by the The limitation of the conversion crystal is generally twice the pulse width of the femtosecond laser used; the time resolution of the femtosecond time-resolved fluorescence loss spectroscopy instrument is very high, but it often only measures one fluorescence wavelength when measuring fluorescence, which reduces its Application in Fluorescence Lifetime Measurement of Weakly Fluorescent Substances

Method used

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  • Integral femtosecond time resolution fluorescence lifetime measurement spectrograph
  • Integral femtosecond time resolution fluorescence lifetime measurement spectrograph
  • Integral femtosecond time resolution fluorescence lifetime measurement spectrograph

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Embodiment 1

[0035] Investigate the time resolution capability of the integrated femtosecond time-resolved fluorescence lifetime measuring spectrometer of the present invention in the performance of the integrated femtosecond time-resolved fluorescence lifetime, adopting 1mmol / L of PBBO dye ethanol solution as test sample , to measure integral femtosecond time-resolved fluorescence lifetime signals. The peak of the absorption spectrum of PBBO is around 320nm, only when the two light pulses of the 490nm pump beam and the 800nm ​​probe beam coincide in space and time at the sample in the sample cell, the fluorescence of PBBO will be excited, thus The time-resolved response function of the integral femtosecond time-resolved fluorescence lifetime measurement spectrometer can be obtained. The instrument response function obtained in this embodiment is fitted with a Gaussian function, and the obtained standard deviation is 0.104 picoseconds, and the full width at half maximum is 0.246 picosecond...

Embodiment 2

[0037] For the investigation of the fluorescence intensity detection limit performance of the integrated femtosecond time-resolved fluorescence lifetime measurement spectrometer described in the invention, the 0.1mmol / L ethanol solution of OX750 dye is used as the sample; the pump light is 490nm, 1.5mW; the probe light is 800nm , 1mW. Measure the integrated femtosecond time-resolved fluorescence lifetime measurement spectrum of the sample, see the attached image 3 . Among them, the left figure (a) is the evolution curve of the measured OX750 fluorescence spectrum with the delay time; the right figure (b) includes the decay curve of the fluorescence intensity at a single wavelength of 660nm without integrating the OX750 fluorescence peak with the delay time (blue (red solid box), and the decay curve of integrated fluorescence intensity versus delay time obtained after integrating the OX750 fluorescence peak (red empty box). It can be seen that the noise of the curve after in...

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Abstract

The invention relates to a time resolution fluorescence spectrometer, in particular to an integral femtosecond time resolution fluorescence lifetime measurement spectrograph. The integral femtosecondtime resolution fluorescence lifetime measurement spectrograph comprises a detection and pump laser light path system, a sample fluorescence collection system and a spectrum detection system. The spectrograph excites a sample through a beam of femtosecond laser, and the other femtosecond laser enables the excited sample to do stimulated radiation; the spectrum detection system integrates collectedsample fluorescence to measure the fluorescence lifetime. The time resolution fluorescence spectrometer improves the detection sensitivity of fluorescence lifetime measurement.

Description

technical field [0001] The invention relates to a time-resolved spectrum measuring instrument, specifically an integral femtosecond time-resolved fluorescence lifetime measurement spectrometer. The spectrometer uses a beam of femtosecond laser to excite the sample, and then uses another beam of femtosecond laser to make the sample in the excited state Stimulated radiation reduces the amount of fluorescence emitted by the free radiation of the sample. The spectral detection system integrates all collected sample fluorescence, and compares the integrated fluorescence intensity with and without stimulated radiation to obtain the fluorescence lifetime signal point. The time delay technology realizes the measurement of the femtosecond time-resolved fluorescence lifetime, and the invention increases the detection sensitivity of the fluorescence lifetime measurement. Background technique [0002] Time-resolved pump-probe technology [Principles and Technical Basis of Ultrafast Laser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6408
Inventor 韩克利孟庆池羊送球
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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