Spectral pupil confocal discrete fluorescence spectrum and fluorescence lifetime detection method and device
A fluorescence lifetime and fluorescence spectrum technology, applied in the direction of measuring devices, fluorescence/phosphorescence, and analytical materials, can solve problems such as interference, inconsistent resolution of the detection system, and collision of the objective lens with the sample to be tested, and achieve high identification speed and identification Accuracy, eliminating the problem of limited measurement accuracy, and the effect of convenient detection process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] The problem to be solved in this embodiment is to simultaneously scan the three-dimensional shape of the sample to be tested and analyze the spatial distribution of the tumor tissue in the sample to be tested, and judge the boundary information of the tumor tissue accordingly. In this embodiment, a confocal detection system is used to measure the three-dimensional shape, and the fluorescence lifetimes of the four wavelengths of 400 nm, 450 nm, 530 nm and 580 nm excited by the 355 nm wavelength pulsed laser in the sample are used to judge each scan Whether the point is a tumor cell or not. Since the fluorescence signal of the sample is very weak, this embodiment uses a photomultiplier tube as a light intensity sensor for fluorescence detection to improve the fluorescence light intensity detection sensitivity of the system.
[0043] Figure 9 It is a specific implementation device of the present invention when realizing confocal discrete fluorescence spectrum and fluores...
Embodiment 2
[0053] Different from Example 1, this example uses the difference in the spectral intensity of fluorescence to determine whether each point on the surface of the sample 8 to be tested is a tumor tissue or a normal tissue. Apparatus used and sample are identical with embodiment 1. In order to improve the stability of fluorescence spectrum measurement, in this embodiment, the light beam emitted by the continuous laser light source 2 is used to excite the sample 8 to generate fluorescence, and the measurement steps are as follows.
[0054] (a) Turn on the continuous laser light source 2, along x and y Move the sample 8 to be tested in the direction to the starting position of the transverse scanning ( x 1 , y 1 ), then at that position along the z Scan the sample 8 to be tested in the direction. Using the confocal detection system 12, it is measured that the variation with the scanning position is as follows: Figure 10 The confocal response curve shown I ( z ), and t...
Embodiment 3
[0060] Different from Example 1, as attached Figure 5 As shown, in order to improve the resolution of the detection beam, a converging lens and a pinhole are respectively added in front of all light intensity sensors. The pinhole is placed at the focus position of the converging lens, and the converging lens converges the fluorescent light beams of different wavelengths to perform spatial filtering through the pinhole. Therefore, the fluorescent signals detected by each light intensity sensor are the filtered fluorescent light intensity information, and these filtered fluorescent lights accurately correspond to the fluorescent signals excited by the focal point of the detection beam, and the fluorescent signals outside the focal point are effectively analyzed. shield.
PUM
Property | Measurement | Unit |
---|---|---|
wavelength | aaaaa | aaaaa |
wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com