161 results about "Autofluorescence" patented technology

The invention relates to an infectious bacterium detection method and system based on double effects of a reflection spectrum and autofluorescence, and belongs to the field of biomedical detection. The method comprises the following steps: acquiring hyperspectral images of different samples under different light sources; performing data preprocessing on the collected sample hyperspectral image, realizing pixel-level alignment of the dual-effect image based on feature matching and image registration, and constructing a data set; the method comprises the following steps: constructing a wound infection bacterium detection model based on double effects of a reflection spectrum and autofluorescence, and comprising a double-branch feature extraction module which comprises a reflection branch and a fluorescence branch which are respectively used for extracting reflection hyperspectral image features and fluorescence hyperspectral image features; the redundant feature screening module screens the features extracted by the reflection branch and the fluorescence branch through a channel attention mechanism; and the feature fusion module adopts a cross-branch cross attention mechanism to fuse the features of the double branches. According to the method, learning is carried out from two physicochemical characteristics, and the effectiveness and adaptability of the technology are improved.

The invention relates to the technical field of crack image analysis, in particular to a building crack width measuring method based on image analysis, which comprises the following steps of: acquiring an autofluorescence attenuation image of a building surface under ultraviolet excitation, and generating a crack response coefficient matrix according to a material aging degree; driving micro-area pulse thermal imaging based on the crack response coefficient matrix, and extracting a heat conduction abnormal boundary as a crack physical edge; surface attachment interference is eliminated through an edge acoustic impedance matching model, and the real width value of the crack is calculated under a material intrinsic coordinate system. According to the method, the normal width of the crack is measured through uniform point selection in the main axis direction, the measured value is subjected to weighted fusion in combination with the local acoustic impedance matching weight, the obtained width result can better reflect the actual structural integrity and elastic continuity of the crack, geometric quantities are provided, and the local damage degree is reflected; the method is suitable for subsequent structure analysis and safety evaluation.

The invention belongs to the technical field of microbiological detection, and discloses a composite probe for detecting salmonella enteritidis and a preparation method. The composite probe for detecting the salmonella enteritidis comprises magnetic nanoparticles FeO, wherein the surface of the magnetic nanoparticles FeO is modified with an aptamer apt which is specifically combined with the salmonella enteritidis; the surface of the long afterglow nano particle PLNPs is modified with a DNA (Deoxyribose Nucleic Acid) sequence cDNA (Complementary Deoxyribonucleic Acid) complementary with the aptamer; the aptamer apt and the complementary DNA sequence cDNA are subjected to hybridization to form a composite probe structure FeO-SEapt (at) PLNPs-cDNA (complementary deoxyribonucleic acid). The long afterglow luminescence characteristic of PLNPs (ZnGaO: Cr) is utilized, and an excitation light source is stopped before detection, so that signal acquisition completely avoids autofluorescence of a sample matrix, background fluorescence interference is thoroughly eliminated, and the signal-to-noise ratio is remarkably improved.

A fluorescence lifetime detection system and a detection method. The fluorescence lifetime detection system includes: a labeling unit for providing quantum dots and for labeling a detection object with the quantum dots; a light emitting unit for providing excitation light, the excitation light being projected onto the detection object labeled with the quantum dots, and the quantum dots being capable of generating fluorescence; a photodetector for detecting the fluorescence generated by the quantum dots and collecting fluorescence signals; and a time-correlated single photon counting unit for obtaining time-based fluorescence lifetime data based on the fluorescence signals. The quantum dots can exhibit different fluorescence lifetimes according to the different microenvironments of the detection object itself. Thus, by detecting the fluorescence generated by the quantum dots, the microenvironment of the detection object can be detected according to the fluorescence lifetime of the quantum dots. Moreover, compared with the autofluorescence of the detection object, the fluorescence lifetime of the fluorescence generated by the quantum dots is longer, and the fluorescence signal obtained by detecting the fluorescence generated by the quantum dots is stronger, which can reduce the detection difficulty and improve the detection accuracy.

Methods of obtaining a stained image of a biological sample using a single channel label -free fluorescence lifetime image of the sample comprising a fluorescence intensity image and associated fluorescence lifetime image as described. The methods comprise obtaining a single channel composite autofluorescence intensity and lifetime image from the single channel label -free fluorescence lifetime image, and providing the single channel composite autofluorescence intensity and lifetime image as input to a deep learning model that has been trained to take as input a single channel composite autofluorescence intensity and lifetime image and provide as output a corresponding stained image. Related methods, systems and products are also described.

Techniques for implementing image normalization for multispectral fluorescence microscopy and virtual staining are disclosed. In an example method, a computing device receives, from an imaging device of a first imaging device type, a first autofluorescence image of a first tissue sample, the first autofluorescence image including one or more imaging channels. The computing device determines one or more normalization parameters for a first channel of the one or more imaging channels, the one or more normalization parameters for the first channel based on a first relationship between the first imaging device type and a second imaging device type, the second imaging device type being different from the first imaging device type. The computing device applies the one or more normalization parameters for the first channel to the first channel of the first autofluorescence image. The computing device outputs the normalized first channel of the first autofluorescence image.

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

A system and method for performing virtual birefringence imaging and virtual staining (e.g., Congo red) of label-free human tissue is disclosed to show that a single trained neural network can rapidly transform autofluorescence images of label-free tissue into brightfield and polarized microscopy images, matching their histochemically-stained versions. Blind testing with quantitative metrics and pathologist evaluations on cardiac tissue showed that the virtually stained polarization and brightfield images highlight amyloid patterns in a consistent manner, mitigating challenges due to variations in chemical staining quality and manual imaging processes in the clinical workflow.

Devices, systems, and methods are provided for imaging cholesteatoma, e.g., using an endoscope including a light source configured to deliver visible light at one or more narrowband wavelengths distally from the endoscope selected to cause autofluorescence in target tissue, a camera coupled to the imaging element to acquire image signals of locations beyond the distal end, and one or more filters configured to remove wavelengths of light in the image signals acquired by the camera including the one or more narrowband wavelengths of visible light.

A system for acquiring data regarding a wound in tissue comprises at least one excitation light source configured to directly illuminate a wound with excitation light; a spectral filtering mechanism configured to permit passage of optical signals responsive to illumination of the wound and having at least one wavelength corresponding to bacterial, fungal, and / or other microorganism autofluorescence and / or bacterial, fungal, viral, and / or microbial fluorescence, the spectral filtering mechanism including a plurality of selectable filters respectively corresponding to different discrete spectral bandwidths; an optical sensor configured to detect the spectrally filtered signals; and a processor configured to receive the detected, filtered signals and to identify a fluorescent signature of bacteria in the wound based at least in part on the detected, filtered signals and to output data regarding the bacterial, fungal, viral, and / or microbial fluorescent signature.

A computer-implemented method for processing an image, comprising: receiving at least one image of an unsliced sample; and processing at least the image by a neural network device for performing digital staining to obtain a digitally stained histological image of the unsliced sample. The image of the unsliced sample is obtained by an imaging system having an excitation light source that provides light to cause one or more substances, such as one or more photoluminescent substances, in the unsliced sample to emit light in order to image the sample. The digitally stained histological image generally corresponds to or substantially corresponds to an image of the unsliced sample that has been chemically stained with one or more chemical stains. The unsliced sample may be an unstained biological sample with an endogenous fluorophore, and the image may be an autofluorescence image of the unstained biological sample.

The invention discloses a difunctional near-infrared fluorescent probe as well as a synthesis method and application thereof. The structural formula of the fluorescent probe is shown as a formula (I). The fluorescent probe DTPD provided by the invention can be used for fluorescent quantitative detection of hypochlorous acid and viscosity, and the fluorescent probe can realize detection of hypochlorous acid with high selectivity in an aqueous solution and is not interfered by other coexisting substances. Besides, the near-infrared fluorescent probe provided by the invention has relatively strong organism penetrability in a near-infrared region, can also reduce the interference of autofluorescence of organisms, and has a good cell imaging effect. And # imgabs0 #.

A method for non-invasively monitoring fluorescent tracers with background separation correction is disclosed, particularly a method for monitoring time-varying fluorescence emitted from a fluorescent agent within a diffusely reflecting medium having time-varying optical properties. The method comprises providing at least two measurements obtained from a patient before and after administration of the fluorescent agent, the measurements comprising a Flr adjacent to the medium detected by a filtered light detector during illumination of the medium with light of an excitation wavelength. meas signal, and at least one DR signal, the DR signal is selected from: #imgabs0#DR em and DR em,filtered The method further includes identifying a post-balanced portion of the measurement data set and assigning each Flr within the post-balanced portion of the measurement data set meas Signal conversion to IF agent signal, the IF agent The signal represents the intensity of the detected fluorescence emitted only by the fluorescent agent in the medium. The disclosed method includes eliminating the effects of leakage of excitation level light and meas Eliminate the influence of autofluorescence in the signal.

This invention discloses a near-infrared long-afterglow nanocomposite, its preparation, and its application in uric acid detection, belonging to the technical field of testing or analyzing materials by measuring their chemical or physical properties. The nanocomposite comprises a near-infrared long-afterglow luminescent center and an outer layer of organosilicon-doped mesoporous silicon containing tetrasulfide bonds. Under near-infrared light excitation, this nanocomposite exhibits excellent afterglow luminescence enhancement response to hydrogen peroxide. Since the product of uric acid decomposition by uricase is hydrogen peroxide, this composite enables highly sensitive and rapid detection of uric acid in blood. The composite provided by this invention has uniform particle size and high stability, effectively eliminating autofluorescence interference from biological components and improving detection accuracy.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The present invention discloses a plant tissue section redyeing method, which relates to the field of biotechnology. The method comprises the following steps: (1) preparing plant tissue sections; (2) observing the plant tissue sections for lignin autofluorescence; (3) subjecting the plant tissue sections observed in step (2) to fluorescent staining for cellulose; and (4) staining the plant tissue sections observed in step (3) with a brightfield dye. The present invention rationally arranges the dyes for use based on whether they are washable. By staining the same sample multiple times, the method is simple and quick, while avoiding errors caused by sample differences.

Systems, instruments, and methods are provided that measure contractility of tissue samples in a parallel and repeatable format based on measurements of optical signals emitted by the tissue samples, e.g., autofluorescence. The tissue samples are imaged during respective contraction periods, capturing the optical signals. In some embodiments, from the images, signal amplitude changes of the optical signals in a region of interest during the contraction period are determined, from which contraction force changes are determined. In some embodiments, from the images, signal amplitude profiles across the region of interest are determined, from which edge translations of fiducial markers and contraction forces are determined.

The present application relates to the technical field of medical image processing, and particularly relates to a combined narrowband and fluorescence imaging method and system for bronchoscopy lesion detection. The method provided by the present application realizes selection and extraction of features with discriminative ability in narrowband imaging images and autofluorescence imaging images based on deep learning, and realizes fusion of advantages of different imaging technologies for lesion detection through decision fusion, thereby improving the speed and accuracy of lesion recognition. Meanwhile, the method provided by the present application can better adapt to different scenes and lesion characteristics, has better generalization ability, improves the specificity and sensitivity of lesion detection, and is beneficial to improving the diagnostic accuracy and efficiency.

Methods and systems described herein detect autofluorescence of a sample. These methods and systems obviate the need for addition of fluorophores to samples to create IR absorption.

The invention discloses a preparation method of a magnetically-driven diatom cell micron robot based on natural diatom shells and a photodynamic therapy method of the magnetically-driven diatom cell micron robot. The method comprises the following steps: firstly, centrifugally enriching a diatom suspension, treating the diatom suspension in a 0.1 M HCl darkroom for 4 hours, removing surface calcium and exposing a porous structure; and carrying out shaking table resonance with positively charged Fe3O4 nanoparticles (300 [mu] g / mL) for 1.5 h, uniformly modifying the magnetic particles on the surface of the shell through electrostatic adsorption, centrifuging, washing, and freeze-drying at-80 DEG C to obtain the Mag-Diatom. The prepared Mag-Diatom not only retains the autofluorescence of natural chlorophyll in diatom and the capability of generating active oxygen through laser triggering, but also can realize directional control and rapid separation in an external magnetic field due to superparamagnetic response of Fe3O4 modification. The invention provides a controllable and directional micro-nano robot PDT platform which has good biocompatibility and a wide clinical application prospect.

The present disclosure provides a system for interrogating tissue comprising a light source for illuminating tissue comprising lesions from ablation and a sensor being configured to receive light to detect autofluorescence from the illuminated tissue. The sensor detects light at a first wavelength in a first wavelength range such that the first wavelength range includes a peak intensity of autofluorescence from the illuminated tissue and detecting light at a second wavelength in a second wavelength range such that the second wavelength range is 20 nm-100 nm longer than the first wavelength range. A processor is programmed to generate a first digital representation of the tissue from light detected in the first wavelength range and a second digital representation of the tissue from light detected in the second wavelength range, and generate a final digital representation from the first and second digital representations. The final digital representation distinguishes between ablated and non-ablated tissue.

The invention relates to a particle analysis system, an information processing method, and a computer readable storage medium. A particle analysis system includes: a light detector that acquires light generated by irradiating particles with excitation light; and an information processing unit that outputs a spectrogram including spectral information of an autofluorescence population specified in a two-dimensional map of measurement data, each measurement data corresponding to the acquired light, and spectral information of the measurement data corresponding to the autofluorescence population specified in the two-dimensional map. And the information processing unit records spectral information of the autofluorescence population as an autofluorescence reference spectrum in the fluorescence separation process.

The present invention relates to the field of substance detection technology, and more particularly to a near-infrared fluorescent probe and its application in the detection of aflatoxin B1. In the present invention, quantum chemical calculation software and molecular dynamics simulation are used to guide the synthesis of near-infrared nanoparticles, and near-infrared nanoparticles are prepared by a microemulsion method. The near-infrared nanoparticles have uniform particle size, good stability, and can emit ultra-high brightness fluorescent signals. By coupling the near-infrared nanoparticles with anti-aflatoxin B1 monoclonal antibodies, they can be used as near-infrared fluorescent signal probes for the detection of aflatoxin B1. Because the near-infrared nanoparticles can achieve short-wave excitation and have a large Stokes shift, they can effectively eliminate interference between excitation light and emitted light, avoiding autofluorescence disturbances. The near-infrared fluorescent signal probe prepared therefrom can significantly improve detection sensitivity.

The utility model relates to the technical field of surgical equipment, and discloses a photoelectric integrated patient interface box and a surgical device. The patient interface box outputs exciting light with a specific wavelength, the exciting light is guided into a detected object through the probe, the detected object generates autofluorescence after being excited by the exciting light, the autofluorescence is guided into the patient interface box through the probe, and the patient interface box obtains a fluorescence value from the autofluorescence and transmits the fluorescence value to the host through the communication cable. Judging whether the detected object is parathyroid gland or not; the host sends out current which passes through the patient interface box and is connected with a tested object through a second wire, the probe contacts with the tested object, and the current is transmitted to the tested object to form a current loop. A detected object generates electromyographic signals after being electrically stimulated, the patient interface box collects the electromyographic signals through the first wire and transmits the electromyographic signals to the host along the cable, electromyographic waveforms are displayed on the host, and whether tissues are recurrent laryngeal nerves or not and whether the recurrent laryngeal nerves are damaged or not are judged through the electromyographic waveforms.

A particle analysis system comprising: a light detector that acquires light generated by irradiating a particle with excitation light; and an information processing unit that outputs a spectral plot including spectrum information of an autofluorescence population specified in a two-dimensional plot of measurement data each of which corresponds to the acquired light and spectrum information of the measurement data and that records the spectrum information of the autofluorescence population as an autofluorescence reference spectrum in a fluorescence separation process.

To provide a micro chamber array that leads solvent flowing through a flow channel excellently to a plurality of housing parts, and can improve the detection sensitivity of fluorescence.SOLUTION: A micro chamber array comprises: a first substrate; a second substrate opposing the first substrate; a flow channel which is provided between the first substrate and the second substrate, and through which solvent containing a sample flows; a chamber body part which is provided on a surface of the first substrate opposing the second substrate; and a plurality of housing parts which are provided for the chamber body part. The chamber body part has a first resin layer and a second resin layer. The second resin layer and the first resin layer are laminated in this order on the first substrate, self-fluorescence created when the second resin layer is irradiated with excitation light is smaller than self-fluorescence created when the first resin layer is irradiated with excitation light, and the contact angle of the second resin layer to water is larger than the contact angle of the first resin layer to water.SELECTED DRAWING: Figure 5

This invention discloses an endoscopic device for thyroid surgery based on fluorescence lifetime and Raman spectroscopy imaging, comprising: a light source assembly, an endoscope probe, a fluorescence lifetime image signal acquisition module, a Raman spectroscopy signal acquisition module, and a color image acquisition module; a host unit, including a control unit and an image processing unit; and a display for displaying an image fused with the Raman spectroscopy signal, the fluorescence lifetime image signal of cancerous tissue, and the color image, as well as an image fused with the Raman spectroscopy signal and the color image. This invention utilizes Raman spectroscopy to visualize the autofluorescence of the parathyroid glands, enabling precise localization of the parathyroid glands. Raman spectroscopy can also locate regional lymph nodes and adipose tissue in the central thyroid region, and combined with fluorescence lifetime imaging, it can provide the conformational state of fluorophores in the neck tissues, marking cancerous tissues and providing real-time optical auxiliary localization for differentiating various anatomical tissues during thyroid endoscopic surgery.

A medical image processing device 9 includes an image processing unit 92 for processing pixel signals acquired from pixels of an imaging element 513. The pixel signal includes: a first pixel signal having an unnecessary light component containing autofluorescence generated by light from a member forming an optical path for observation when light emitted from the light source device propagates through the optical path for observation, and an observation target fluorescence component containing fluorescence generated by the member forming the optical path for observation when light emitted from the light source device propagates through the optical path for observation; the observation object fluorescence component is emitted from a substance contained in the observation object excited by light; and a second pixel signal having at least an unnecessary light component. The image processing unit 92 includes a signal correction unit 923 that generates a corrected pixel signal on the basis of the first and second pixel signals and a correction coefficient set on the basis of spectral characteristics of fluorescence to be observed.

The invention relates to the field of optical fiber detection, and discloses an optical fiber bundle autofluorescence intensity detection method, medium and device, and the method comprises the steps: obtaining an end face image of a to-be-detected optical fiber bundle, extracting connected domains of the to-be-detected optical fiber bundle, and generating a defect judgment result of each connected domain; acquiring an end face image of the to-be-detected optical fiber bundle under each illuminance level; extracting a brightness value of a non-defective target connected domain in each end face image, and screening out a target brightness mean value under each illuminance level according to a preset condition; and associating each target brightness mean value with the corresponding optical power, and fitting to generate a curve that the autofluorescence brightness changes along with the optical power. The method is accurate in detection result, can adapt to optical fiber bundles with different lengths and different transmittances, can select a fluorescent contrast agent in a proper concentration range according to the detection result, reduces the interference of background noise on a fluorescent signal corresponding to the fluorescent contrast agent, and further improves the accuracy of a diagnosis result.

A method is provided for detecting and displaying autofluorescence signals during a surgical procedure using a multimodality probe. The method comprises a control unit receiving a first signal from a handheld multimodality probe, the first signal indicating activation of an autofluorescence detection component of the probe in an operating room environment. The method also comprises the autofluorescence detection component illuminating tissue using an excitation light source in the probe. The method also comprises the control unit receiving autofluorescence emission data, the emission data corresponding to native fluorophores present in the tissue. The method also comprises the control unit processing the emission data to generate at least one of an intensity map and an image corresponding to strength of autofluorescence signal. The method also comprises the control unit displaying, on at least a display screen in the operating room, the autofluorescence data.