93 results about "Autofluorescence" patented technology

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

Devices, systems, and methods are provided for imaging cholesteatoma, e.g., using an endoscope including a light source configured to deliver visible light at one or more narrowband wavelengths distally from the endoscope selected to cause autofluorescence in target tissue, a camera coupled to the imaging element to acquire image signals of locations beyond the distal end, and one or more filters configured to remove wavelengths of light in the image signals acquired by the camera including the one or more narrowband wavelengths of visible light.

This invention discloses a near-infrared long-afterglow nanocomposite, its preparation, and its application in uric acid detection, belonging to the technical field of testing or analyzing materials by measuring their chemical or physical properties. The nanocomposite comprises a near-infrared long-afterglow luminescent center and an outer layer of organosilicon-doped mesoporous silicon containing tetrasulfide bonds. Under near-infrared light excitation, this nanocomposite exhibits excellent afterglow luminescence enhancement response to hydrogen peroxide. Since the product of uric acid decomposition by uricase is hydrogen peroxide, this composite enables highly sensitive and rapid detection of uric acid in blood. The composite provided by this invention has uniform particle size and high stability, effectively eliminating autofluorescence interference from biological components and improving detection accuracy.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The present disclosure provides a system for interrogating tissue comprising a light source for illuminating tissue comprising lesions from ablation and a sensor being configured to receive light to detect autofluorescence from the illuminated tissue. The sensor detects light at a first wavelength in a first wavelength range such that the first wavelength range includes a peak intensity of autofluorescence from the illuminated tissue and detecting light at a second wavelength in a second wavelength range such that the second wavelength range is 20 nm-100 nm longer than the first wavelength range. A processor is programmed to generate a first digital representation of the tissue from light detected in the first wavelength range and a second digital representation of the tissue from light detected in the second wavelength range, and generate a final digital representation from the first and second digital representations. The final digital representation distinguishes between ablated and non-ablated tissue.

The invention relates to a particle analysis system, an information processing method, and a computer readable storage medium. A particle analysis system includes: a light detector that acquires light generated by irradiating particles with excitation light; and an information processing unit that outputs a spectrogram including spectral information of an autofluorescence population specified in a two-dimensional map of measurement data, each measurement data corresponding to the acquired light, and spectral information of the measurement data corresponding to the autofluorescence population specified in the two-dimensional map. And the information processing unit records spectral information of the autofluorescence population as an autofluorescence reference spectrum in the fluorescence separation process.

This invention discloses an endoscopic device for thyroid surgery based on fluorescence lifetime and Raman spectroscopy imaging, comprising: a light source assembly, an endoscope probe, a fluorescence lifetime image signal acquisition module, a Raman spectroscopy signal acquisition module, and a color image acquisition module; a host unit, including a control unit and an image processing unit; and a display for displaying an image fused with the Raman spectroscopy signal, the fluorescence lifetime image signal of cancerous tissue, and the color image, as well as an image fused with the Raman spectroscopy signal and the color image. This invention utilizes Raman spectroscopy to visualize the autofluorescence of the parathyroid glands, enabling precise localization of the parathyroid glands. Raman spectroscopy can also locate regional lymph nodes and adipose tissue in the central thyroid region, and combined with fluorescence lifetime imaging, it can provide the conformational state of fluorophores in the neck tissues, marking cancerous tissues and providing real-time optical auxiliary localization for differentiating various anatomical tissues during thyroid endoscopic surgery.

A medical image processing device 9 includes an image processing unit 92 for processing pixel signals acquired from pixels of an imaging element 513. The pixel signal includes: a first pixel signal having an unnecessary light component containing autofluorescence generated by light from a member forming an optical path for observation when light emitted from the light source device propagates through the optical path for observation, and an observation target fluorescence component containing fluorescence generated by the member forming the optical path for observation when light emitted from the light source device propagates through the optical path for observation; the observation object fluorescence component is emitted from a substance contained in the observation object excited by light; and a second pixel signal having at least an unnecessary light component. The image processing unit 92 includes a signal correction unit 923 that generates a corrected pixel signal on the basis of the first and second pixel signals and a correction coefficient set on the basis of spectral characteristics of fluorescence to be observed.

The invention discloses preparation of a low-background-leakage microarray detection system for catalyzing a hairpin self-assembly reaction in combination with time-resolved resonance energy transfer and application of the microarray detection system in esophageal cancer diagnosis, and belongs to the technical field of biomedical detection. The probe combination provided by the invention comprises a probe pair for detecting miRNA-21, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.1 and SEQ ID NO.2; the nucleotide sequences of the probe pair for detecting the miRNA-25 are respectively SEQ ID NO.3 and SEQ ID NO.4; and / or a probe pair for detecting the miRNA-93, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.5 and SEQ ID NO.6. TMGA is added in a reaction system, the thermal stability of the probe H1 / H2 is enhanced through combination of positive ions of TMGA and DNA small grooves, so that background leakage caused by respiration is inhibited, a time-resolved fluorescence technology is adopted, and serum short-life autofluorescence is avoided by delaying measurement time.

Translational rapid ultraviolet-excited sectioning tomography (TRUST) applies ultraviolet (UV) excitation to a sample and images fluorescence and autofluorescence emission for tomographically imaging the sample. Deep-learning neural networks are used to achieve higher imaging speed and imaging resolution. In one use, fluorescence images acquired with relatively low imaging resolution can be transformed into high-resolution images through the first conditional generative adversarial network (cGAN), a super-resolution neural network (e.g., ESRGAN), which is also helpful for reducing the image scanning time. In another use, the second cGAN, such as Pix2Pix, is used to realize virtual optical sectioning to enhance the axial resolution of the imaging system. Compared to the conventional pattern illumination methods (e.g., HiLo microscopy), which need at least two shots for each field of view, the imaging speed is also times higher because only one shot under the uniform-illumination condition of UV irradiation is required.

A device for detecting bacteria in a sample comprises an optical block (4) arranged to produce multispectral image sets of portions of a sample, obtained by illumination eliciting an autofluorescence response and by illumination eliciting a reflectance response. These multispectral image sets are provided to an analyzer (6) comprising a classifier that produces an image in which each pixel is derived from an identical pixel in the images of the multispectral image set and is associated with an element identifier indicating whether that pixel is associated with a bacterium or another element. This image is then used by a computer to determine bacteria detection data. Fig. 1

The invention relates to a device for detecting bacteria in a sample, the device comprising an optical unit (4) arranged to produce sets of multispectral images of portions of a sample, these being obtained by means of illumination eliciting an autofluorescence response and by means of illumination eliciting a reflectance response. These sets of multispectral images are fed to an analyser (6) comprising a classifier that produces an image, each pixel of which is drawn from an identical pixel in the images of the set of multispectral images and is associated with an element identifier indicating whether this pixel is associated with a bacterium or with another element. This image is then used by a computer to determine bacteria detection data.

This invention discloses a magnetic fluorescently imprinted nanoparticle, its preparation method, and its application. The nanoparticle comprises amino-functionalized magnetic nanoparticles and a fluorescently imprinted polymer layer coated on their surface, wherein the fluorescently imprinted polymer layer has an imprinted cavity; the imprinted molecule in the fluorescently imprinted polymer layer is a cTnI-specific C-terminal epitope peptide. Functional monomers are screened using computational chemistry, and nanomolecularly imprinted polymers (MIPs) are synthesized using epitope imprinting technology. Fluorescent monomers are introduced to impart autofluorescence response properties, forming a label-free detection system. These nanoparticles exhibit highly efficient adsorption and specific recognition of cTnI protein, with a detection sensitivity of 1 pg / mL (R²=0.98). They show extremely low non-specific adsorption to common interfering proteins and effectively resist interference from complex biological matrices, providing a new approach for rapid clinical cTnI detection.

The application is suitable for the technical field of image processing, and provides a multi-modal image display optimization method and device and terminal equipment, which comprises the following steps: obtaining an OCT image and a autofluorescence image of a target blood vessel section, the OCT image being an image obtained through optical coherence tomography, and the autofluorescence image being an image obtained through autofluorescence of a specific plaque component stimulated by near-infrared laser, fusing the OCT image and the autofluorescence image to obtain a target image, each pixel point in the target image being determined according to each pixel point of the OCT image, and the value of each pixel point in the target image being determined according to the autofluorescence intensity coefficient of a pixel point of the autofluorescence image. The application can improve the accuracy of identifying an atherosclerotic plaque in the OCT image.

The invention provides a dyeing method for removing autofluorescence of a single cell sorting micro-fluidic chip and a chip without autofluorescence. The dyeing method comprises the following steps: completely soaking the single-cell sorting micro-fluidic chip in a dye solution for dyeing until the top layer of the chip is dyed into a non-transparent black top layer, thereby obtaining the single-cell sorting micro-fluidic chip without autofluorescence, the dye in the dye solution is black dye; the single cell sorting micro-fluidic chip at least comprises a photosensitive dry film serving as a runner layer. The staining method provided by the technical scheme of the invention can effectively solve the problem of autofluorescence of a single cell sorting micro-fluidic chip prepared from a traditional photosensitive dry film, so that when the stained chip is applied to fluorescence activated cell sorting, recognition of automatically screened fluorescent cells is facilitated, the operation difficulty of fluorescence activated cell sorting is reduced, and the working efficiency is improved. And accuracy and reliability are improved.

The detection of the micro-plastic needs to be combined with various technologies. For example, position detection techniques such as cross polarization detection, autofluorescence detection or photo-thermal infrared imaging are utilized to determine the position of the microplastics in a sample. The microplastics are then characterized by detecting the infrared absorption at these locations. Compared with the prior art, the method has the advantage that the content of the micro-plastics can be positioned and represented more quickly and more accurately.

A method and system for assessing a histopathological cause of kidney graft dysfunction in a subject following kidney transplantation is disclosed. The method comprises the steps of: a) generating one or more images of the native fluorescence emission from one or more exfoliated kidney cells obtained from a urine sample from the subject in a plurality of distinctive spectral channels, b) calculating, for each urinary exfoliated kidney cell, quantitative features of the autofluorescence signals in said one or more images, c) applying a feature selection methodology to the quantitative features to generate a set of optimised quantitative features; and d) applying a classification method to the optimised quantitative features to produce a multispectral profile for differentiating the histopathological cause of kidney graft dysfunction in the subject from a plurality of histopathological causes of kidney graft dysfunction.

An electrophoresis apparatus with minimal autofluorescence enabling in-situ gel processing, and a method for manufacturing and using the electrophoresis apparatus. An exemplary electrophoresis apparatus may include a housing defining a cavity between a first and a second pane of a double-layered observation window, and may further include a plate-like gel located within the cavity. The observation window may be transparent to ultraviolet light driving derivatization reactions in the plate-like gel, and in the absence of the plate-like gel, may define window autofluorescence induced by ultraviolet light irradiation. Under the same unit area and the same ultraviolet light irradiation, the window autofluorescence per unit area may be less than 5 times that of the plate-like gel autofluorescence.

Detection of microplastics is accomplished using a combination of techniques. A position-detection technique such as crossed-polarization detection, autofluorescence detection, or photothermal infrared imaging is used to determine the locations of microplastics in a sample. Infrared absorption can be detected at those locations to characterize the microplastics. In this way the microplastic content can be located and characterized more quickly and accurately than using conventional techniques.

The invention belongs to the field of fluorescent probes, and discloses a selenium cyano fluorescent probe for high-selectivity recognition of H2S as well as a preparation method and application of the selenium cyano fluorescent probe. The probe adopts dicyanoisophorone as a fluorophore skeleton and-SeCN group as a novel recognition site, and the structural formula is shown in the specification. The dicyanoisophorone has relatively large Stokes shift during release, so that the interference of autofluorescence on signal detection is reduced, and the dicyanoisophorone is connected with a-SeCN group through a condensation reaction. On the basis of a double nucleophilic substitution strategy, H2S induces conversion of-SeCN into-Se-S, and near-infrared fluorophores are released through intramolecular self-cyclization. Researches show that the probe has high sensitivity, selectivity, good water solubility and thiol consumption resistance, the detection limit is as low as 20 nM, and the probe has good ratio signal and brain targeting ability.

This invention discloses a short-wave infrared fluorescence endoscopic imaging device and method. Based on a pre-made near-infrared endoscope, the device achieves dual-path multiplexing of the original imaging and illumination channels. Specifically, the imaging channel performs white light imaging and excitation light scanning, while the illumination channel performs white light illumination and short-wave infrared fluorescence collection. The excitation light source module is connected to the scanning module, and the scanning and white light imaging modules are connected to the endoscope via a dichroic mirror. The short-wave infrared detection module and white light illumination module are connected to the endoscope via a multi-integrated fiber bundle. The control and data acquisition module is electrically connected to the scanning module, short-wave infrared detection module, and white light imaging module, respectively. This invention eliminates the need to modify the endoscope's structure, overcomes the technical bottleneck of pre-made endoscopes being unable to perform short-wave infrared fluorescence imaging, and achieves deep fusion of white light and short-wave infrared fluorescence dual-modal imaging. This effectively reduces scattering and autofluorescence interference, and improves the tissue penetration depth and signal-to-background ratio of endoscopic imaging.

An information processing device according to an aspect of the present disclosure includes a simulated image generation unit (131a) that generates a simulated image by superimposing a non-stained image including an autofluorescence component and a dye tile image in which a reference spectrum of a first fluorescent dye and imaging noise of each of pixels of the non-stained image are associated, a fluorescence separation unit (131b) that separates a component of the first fluorescent dye and the autofluorescence component on the basis of the simulated image and generates a separated image, and an evaluation unit (131c) that evaluates a degree of separation of the separated image.

Super-resolution label-free microscopy is provided using multiplexed, temporally modulated acquisition patterns of emission point spread functions (“PSFs”). Supercontinuum ultrafast pulses can be used to enhance nonlinear processes, such as autofluorescence and harmonic generation, in order to provide super-resolution imaging of nonlinear label-free signals. Images can be reconstructed using various reconstruction techniques, including pixel reassignment, wavelet reconstruction, and deep learning model-based reconstructions.

The invention discloses a pH proportion type fluorescent probe of an indole derivative, a synthesis method and application, and belongs to the technical field of fluorescent probe detection. Experiments show that the maximum excitation wavelength (620nm) and the maximum emission wavelength (650nm) of the probe HKP can effectively reduce biological sample damage caused by short wavelength excitation, and the background interference of biological autofluorescence is remarkably reduced, so that the probe has the advantages of high sensitivity, high sensitivity and the like. Quantification can be carried out by measuring the intensity ratio of fluorescence with two different wavelengths, and interference of environmental factors such as probe concentration, illumination intensity and cell thickness can be effectively eliminated, so that more accurate pH measurement is realized. The probe is highly sensitive to pH based on a hydroxy ketone interconversion structure, the fluorescence intensity of the probe has remarkable and reversible response along with the change of the pH value of the environment, and the probe has excellent stability, high selectivity and good biocompatibility.

This invention introduces an improved method for measuring macular pigment optical density (MPOD) using autofluorescence imaging (AFI). Traditional AFI methods often neglect melanin absorption, leading to inaccuracies. The proposed method employs three wavelengths: 488 nm for the main image, 514 nm as a traditional baseline, and 600 nm as an additional baseline to account for melanin absorption. By converting the melanin absorption coefficient from 600 nm to 488 nm and 514 nm, this method ensures precise MPOD measurements. Additionally, a simplified dual-wavelength method is introduced using 488 nm and 600 nm for scenarios requiring less complexity. Both approaches enhance the accuracy of non-invasive retinal imaging by providing a more reliable tool for assessing macular pigment levels and improving our understanding of retinal health.

The invention discloses an intraoperative parathyroid gland recognition and blood supply monitoring method and system based on a laser speckle contrast technology, relates to the technical field of intraoperative parathyroid gland recognition, and solves the problems that in the prior art, parathyroid gland recognition is difficult, the intraoperative mistaken cutting risk is high, and blood flow detection is insufficient. The method comprises the following steps: acquiring an autofluorescence image and a speckle contrast image, carrying out noise reduction and image enhancement processing on the autofluorescence image, and evaluating the blood vessel distribution and blood flow velocity of the identified parathyroid gland according to the speckle contrast of the speckle contrast image, according to the autofluorescence image and the speckle contrast image, the position and the form of the parathyroid gland are manually recognized, the autofluorescence image and the speckle contrast image are input into a pre-trained neural network model to obtain an AI recognition result, and by combining LSCI, NIRAF and AI technologies, real-time recognition and blood supply state monitoring of the parathyroid gland are achieved. And objective blood supply state evaluation and automatic parathyroid gland identification can be provided by using the neural network model.

Methods and systems described herein detect autofluorescence of a sample. These methods and systems obviate the need for addition of fluorophores to samples to create IR absorption. An IR source and an excitation source are used to generate emission spectra of the chosen sample, which are then analyzed to determine various data about the sample.

A method of analyzing a tissue is provided that includes: administering a fluorescent agent to a tissue; producing a first excitation light that is configured to produce a fluorescence emission from the fluorescent agent administered to the tissue; producing a second excitation light that is configured to produce an autofluorescence emission from a biomolecule of interest present within the tissue; using a photodetector to detect the fluorescence emissions and produce first signals representative thereof, and produce second signals representative of the autofluorescence emission; producing an image using the first and second signals. The image includes a first, second and third portions representative of tissue types present within the tissue; and analyzing the tissue to identify diseased tissue and to distinguish it from the first and second tissue types.

This invention relates to the field of biodetection technology, specifically disclosing a multiplex fluorescent staining kit for tissue samples. The kit includes an antibody dissociation buffer, a signal amplification deposition solution, a fluorescent labeling reagent, a tissue-protective imaging medium, and an autofluorescence background inhibitor. This invention utilizes a non-destructive elution technique achieved through the synergistic effect of a low-pH buffer and a directional electric field. It leverages the positively charged nature of antibodies under acidic conditions to drive them away from the tissue and towards the negative electrode, achieving gentle and efficient antibody removal. Simultaneously, it employs a two-step cascade amplification strategy of small molecule deposition and copper-free click chemical labeling, balancing deep penetration with ultra-high sensitivity. This invention can stably support 6-12 cycles of staining, exhibits high elution efficiency, preserves tissue morphology, and can detect low-abundance targets. It is suitable for spatial analysis of the tumor microenvironment, monitoring of transplant immune rejection, and pathological evaluation of neurodegenerative diseases, demonstrating promising clinical application prospects.

A device for measuring intrinsic autofluorescence of molecules present in a biological sample wherein the device includes: a) a light-sheet, virtual light-sheet or light line delivery pathway module including a laser generator capable of generating ultrashort pulses with a duration between 1 microsecond and 1 attosecond, and optical elements shaping the laser light into a light sheet, virtual light-sheet or light-line; b) an imaging chamber including an imaging dish inside of which the biological sample is placed, a translational stage, an illumination objective and a collection objective both immersed in an immersion liquid in a sealed immersion chamber, wherein both objectives are positioned below the imaging dish, wherein the immersion chamber is placed inside an incubation chamber, allowing environmental control; and c) a fluorescence detection pathway including optical elements, light-splitting elements such as diffractive elements or dichroic mirrors, an array detector, and a device control and signal processing unit.