The invention provides a composition of instant freeze-dried fibrinogen and thrombin preparation, a preparation method and a use thereof, and the composition comprises composition 1 composed of 35-70mg/ml of fibrinogen, 9-15mg/ml of sodium citrate, 7-12mg/ml of sodium chloride, 0.3-0.6mg/ml of polysorbate-80, 10-15mg/ml of mannitol, 4 -8mg/ml of arginine and 3.5-5.5mg/ml of glutamic acid by mg/ml and composition 2 composed of 700-1,200 mg/ml of thrombin, 3-6mg/ml of dextran 20, 15-25mg/ml of glycine, 5-7mg/ml of sodium chloride and 4-7mg/ml of calcium chloride by mg/ml. A sealant avoids the risk of spreading of AIDS virus, and the like, increases the drug stability, reduces the degeneration during the virus inactivation process by dry-heat method, protects biological activity, avoids local liquid storage of the using part caused by high permeability and the irritation of a large amount of inorganic salts to tissues, is conductive to the healing of trauma sites and ensures product safety and independent package to the maximum extent, thereby increasing storage time, facilitating use and meeting the clinical and field first-aid needs.