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Bacillus subtilis YN145 and application thereof

A technology of Bacillus subtilis and YN145, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of reduced anti-microbial control effect, unsatisfactory effect, poor colonization ability of antagonistic microbes, etc., and achieve good control effect, protection and treatment The effect of both functions and avoiding adverse effects

Active Publication Date: 2016-12-07
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the field of biological control, there are often problems such as poor colonization ability of antagonistic microorganisms under natural conditions in the field and unstable effect on disease control.
A large number of studies have also shown that the antagonistic microorganisms screened indoors have a strong inhibitory effect on pathogenic bacteria in the greenhouse, but the effect in field control is not satisfactory. Colonize the root circle of plants, on the other hand, interact with the complex microbial species and antagonistic strains in the field soil, resulting in a decrease in the control effect of antagonistic microorganisms. Therefore, it is necessary to select and screen biological strains with strong field adaptability or high-efficiency metabolites Applying to field disease control is an important way to solve the above problems

Method used

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  • Bacillus subtilis YN145 and application thereof
  • Bacillus subtilis YN145 and application thereof
  • Bacillus subtilis YN145 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The separation and acquisition of Bacillus subtilis YN145

[0029]Healthy rice plants were selected from diseased clusters of rice blast-susceptible rice variety Xiangzaoxian 24 (N28°42′55.56″, E112°15′74.24″) in Huatian Village, Shiniujiang Town, Taojiang, Hunan Province, Hunan Province, and a total of 517 plants were isolated. An antagonistic bacteria YN145 was screened out by plate confrontation method against Magnaporthe grisea (M. oryzae) with an inhibition rate of 84.31%.

[0030] By culturing bacterial strain YN145 on NA solid medium (peptone 5g / L, beef extract 3g / L, glucose 20g / L, agar 17g / L, pH7.0, distilled water preparation) plate ( figure 2 A and figure 2 B), observe the colony morphology, observe the scanning electron microscope images of the bacteria, and combine the physiological and biochemical and microbiological detection results of the bacteria, and the 16S rDNA sequencing results (see SEQ ID NO.1), to finally determine the bacterial stra...

Embodiment 2

[0032] Example 2 Application of Bacillus subtilis YN145 in the Control of Rice Blast

[0033] After the strain YN145 was activated on the NA solid medium plate, two bacterial cakes with a diameter of 5mm were punched out and inserted into 150mL NB liquid medium (peptone 5g / L, beef extract 3g / L, glucose 20g / L L, pH 7.0, prepared with distilled water) in a 250mL Erlenmeyer flask, cultured in a shaker at 28°C and 180r / min for 4 days, and filtered with a bacterial filter to obtain the metabolite of Bacillus subtilis YN145.

[0034] Mix the metabolites of bacterial strain YN145 and PDA medium at a volume (mL) of 1:8, connect the rice blast fungus cake with a diameter of 7mm on the prepared medium, and replace the antagonistic bacteria culture solution with sterile water for Control, cultured at 28°C, measured the size of the fungus cake of Magnaporthe oryzae after 10 days, repeated 3 times, the results showed that the inhibition rate of Mycelium of Magnaporthe oryzae was 84.31%.

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Abstract

The invention relates to the technical field of biological control and particularly discloses a bacillus subtilis YN145 and application thereof to rice blast prevention. The preservation number of the bacillus subtilis YN145 is CCTCC No:M2016339. The strain YN145 is used for preventing and treating rice blast, so potential bad influences of chemical prevention on the environment and rice safety and susceptible risks of utilization of disease-resistant varieties are avoided. Living bacteria and metabolite of the strain YN145 have a good prevention effect, has the prevention and treatment effects, and can be used for processing and development of living bacteria inoculant and metabolite preparations. Besides, the strain YN145 has inhibition effects on various pathogenic indicators.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a bacillus subtilis YN145 and its application in preventing and treating rice blast. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops, and more than half of the world's population feeds on rice. Rice blast (the pathogen is Magnaporthe oryzae, and its asexual generation is Pyiicularia oryzae) is one of the most serious fungal diseases of rice. M. oryzae is a semi-living parasite with a distinct biotrophic phase during the initial stages of infection. Rice blast can be divided into seedling blast, leaf blast, knot blast, panicle blast, grain blast, and branch blast according to the location and time of onset. At present, the production mainly uses disease-resistant varieties and chemical fungicides to control rice blast. The main fungicides used are tricyclazole, rice blastling, prochloraz, rice blastamide, isoframine, rice bla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/125
CPCA01N63/00A01N63/10C12N1/205C12R2001/125
Inventor 刘二明邹秋霞任佐华刘敏捷周鑫钰刘双清
Owner HUNAN AGRICULTURAL UNIV
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