30 results about "Indicator bacteria" patented technology

The invention relates to a microbial index-based estuary and coastal environment ecological evaluation method, which comprises the following steps of: selecting representative sampling points, collecting sediment samples, analyzing and evaluating pressure gradients generated by human activities to estuary and coastal environments through physicochemical indexes, and constructing pressure indexes according to a reference standard, the comprehensive environment pressure index of each sampling point is constructed and calculated to reflect the overall condition of the target area environment; evaluating the health condition of large invertebrate communities in estuary and coastal environments and the response of the communities to human pressure, and calculating a marine organism index AMBI, so as to display the ecological health condition of a region; a high-throughput sequencing technology is used for identifying microorganism composition, pollution indicator bacteria are defined, a microorganism index M-AMBI value is calculated according to the pollution indicator bacteria, and the ecological health condition of each sampling point is graded; and finally, verifying the reliability of an ecological evaluation result through statistical analysis. According to the method, multi-dimensional data are integrated, the ecology of the estuary and coastal environment is comprehensively evaluated, a scientific basis is provided for revealing an environment pressure source and an ecological effect thereof, formulation of a precise environment management strategy is assisted, pollution risks are effectively prevented and controlled, and sustainable development of an estuary and coastal ecological system is promoted.

The invention discloses a method for screening bacterial strains with an antibacterial function, which comprises the following steps: to-be-screened bacteria and indicator bacteria are screened in a microfluidic system, the indicator bacteria have a fluorescent label so as to obtain target bacteria, and the target bacteria have the activity of inhibiting the indicator bacteria. According to the method, the antagonistic activity of the strains is associated with the fluorescence signal intensity, the functional strains with the antibacterial function are screened in a high-throughput and high-precision mode, the screening throughput reaches 106-108 strains every day, and therefore the screening efficiency and the result stability are remarkably improved.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present application relates to the technical field of animal health monitoring, and relates to a wild animal non-invasive genome monitoring and population health evaluation system. A time indication marker is generated based on the ratio of a predetermined indicator bacteria genus, and microenvironment data is input into a pre-trained microbial succession model to intelligently infer the actual exposure time of the sample in the wild. Then, the initial intestinal flora map of the sample at the instant of discharge is reconstructed. The high-fidelity flora map after reconstruction is further deeply coupled with health evaluation to realize scientific diagnosis and early warning of the physiological state of wild animal individuals. The accuracy of health evaluation, nutritional status analysis and pathogen detection is greatly improved, and major misjudgments such as misjudging environmental bacteria as pathogenic bacteria or misdiagnosing flora natural decay as ecological imbalance are avoided. A stable and reliable technical tool is provided for wild animal protection management.

The invention discloses a strain of Lactobacillus gasseri for relieving diabetes symptoms and its application, belonging to the field of microbial technology, wherein the Lactobacillus gasseri is named as Lactobacillus gasseri FLG‑219, with a deposit number of CGMCC No.28338, and has a relatively good relieving effect on various case indicator bacteria of type 2 diabetes. The probiotic composition finally provided by the invention can greatly improve the relieving effect of probiotics on two core indicators of type 2 diabetes, making the effect of probiotic preparations closer to the efficacy of drugs, which is of great significance to the clinical treatment of type 2 diabetes.

The invention relates to the technical field of new application of a natural orange peel extract, in particular to a bacteriostatic agent of the natural orange peel extract and application of the bacteriostatic agent. Bacteriostatic experiments find that the natural extract hesperetin and / or hesperidin of the orange peel has a good bacteriostatic effect on most of aquaculture indicator bacteria. Animal experiments after the hesperetin and / or hesperidin are prepared into the disease-resistant feed find that the hesperetin or hesperidin can effectively improve the resistance of fishes to streptococcus iniae infection. A 5 * MIC hesperetin group shows the best protection effect, the protection rate reaches 60%, and the protection rate of streptococcus iniae fed with a hesperidin group is 46%. Therefore, the hesperetin and / or the hesperidin which is the natural extract of the orange peel can be used as a bacteriostatic agent or a bacteriostatic feed in aquaculture, new application of the hesperetin and the hesperidin in aquaculture is developed, and the hesperetin and the hesperidin have important significance in controlling and preventing streptococcicosis.

The application discloses a royal jelly quality evaluation method based on bacteriostatic activity, and belongs to the technical field of bioengineering and food quality evaluation. The method evaluates the quality of royal jelly by determining the bacteriostatic activity of the royal jelly. Specifically, the royal jelly to be measured is diluted to 10% volume concentration, then the bacteriostatic activity of the diluted royal jelly on indicator bacteria is determined by adopting a filter paper method, and the quality grade of the royal jelly to be measured is determined according to the bacteriostatic ring diameter. The quality of the royal jelly is evaluated by determining the bacteriostatic activity of the royal jelly, the influence of water content, 10-HDA and freshness on the quality of the royal jelly is considered in principle, the method has the characteristics of simple operation and intuitive evaluation, and is easy to popularize and apply, thereby laying an important foundation for the quality detection technology of royal jelly.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention discloses a nisin titer detection method based on a microwell plate method. According to the growth inhibition effect of nisin on indicator bacteria, a plant lactobacillus L30 with a good strain growth amount-nisin titer curve is screened from existing strains, the plant lactobacillus L30 is applied to a microwell plate method, and a nisin titer determination method which is high in accuracy, convenient and economical is developed.

Loop-mediated isothermal amplification assays, kits, and methods that target and / or detect the presence of a fecal indicator bacteria in a sample. These assays, kits, and methods can be portable and capable of providing fast (within 60 minutes) results in the field, eliminating the need for a laboratory and other complex equipment.

The invention discloses an antibacterial peptide as well as a preparation method and application thereof in preparation of a preparation for treating pneumonia, and belongs to the technical field of biological medicines. The antibacterial peptide is a mutant of endolysin derived from gram negative bacteriophage, and the endolysin of the bacteriophage plays a bacteriostatic / bactericidal role by degrading bacterial cell walls, and basically does not generate drug resistance. Common pneumonia pathogenic bacteria including moraxella catarrhalis, streptococcus pneumoniae and staphylococcus aureus are used as indicator bacteria, three antibacterial peptide mutants EAMP-M1, EAMP-M2 and EAMP-M3 with good antibacterial effects are obtained through screening, and experiments prove that the antibacterial peptide mutants have good antibacterial effects on gram-positive bacteria and gram-negative bacteria; the composition can be applied to prevention, treatment or auxiliary antibiotic treatment of common pneumonia infection; and the disinfectant is used in the field of medical instruments, such as surgical instrument disinfection, and has a wide and good market application prospect.

The invention relates to bacillus siamensis and an application of the bacillus siamensis in aquaculture. The bacillus siamensis is obtained by carrying out antagonistic strain separation and screening by taking vibrio parahaemolyticus as an indicator bacterium. Through verification, the strain has a relatively obvious inhibition effect on vibrio parahaemolyticus causing vibriosis in aquaculture, also has certain resistance to pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, in addition, the strain has relatively good high-temperature resistance, acid and alkali resistance and salt tolerance, and when the strain or a microbial agent prepared from the strain is applied to aquaculture, the yield of aquaculture is greatly increased. The compound has high inhibition rate on various germs including vibrio parahaemolyticus in a water body, does not influence the water body environment, has potential effects of promoting the growth of bred animals, stimulating the response of an immune system, improving the immunity, improving the water quality condition, repairing the ecological environment and the like, and has a wide application prospect.

The invention discloses trichoderma for efficiently preventing and controlling tobacco root rot and a culture method thereof, which are characterized in that tobacco root rot biocontrol bacteria are separated from tobacco rhizosphere soil, tobacco root rot pathogenic bacteria share fusarium F. Commune as indicator bacteria, and bacterial strains with bacteriostatic action on the growth of fusarium solani are screened to obtain the trichoderma LB8-12-2; the method can effectively inhibit the tobacco from being infected by fusarium pathogenic bacteria in the growth process, and can greatly improve the survival rate and quality of the tobacco in the growth process when being applied to the tobacco growth process. Experimental data show that after tobacco is treated by the trichoderma LB8-12-2, the relative control effect is 95.51%, and it is indicated that the trichoderma LB8-12-2 has a good control effect on the tobacco fusarium root rot, so that the trichoderma LB8-12-2 has remarkable advantages in the aspects of protecting the environment, reducing pesticide residues and improving the safety of tobacco leaves when being used for controlling the tobacco fusarium root rot, and the application prospect is wide.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

A motility analysis system is disclosed for identifying the presence and type of bacteria via motility-based diagnostic testing. In an example, the motility analysis system receives video data of a fluid sample containing bacteria captured through a microscope, generates a two-dimensional (2D) time-projection of motile tracks of the bacteria based on a pixel-by-pixel analysis of the video data, processes the 2D time-projection of the motile tracks with a quantitative analysis model to identify a match between the 2D time-projection of the motile tracks and a plurality of predetermined 2D time-projections, and outputs data indicative of an identity of the bacteria in response to identifying the match. Certain examples can also perform such testing before and after application of antibiotics to evaluate resistance based on motility. Attachment devices for adapting computing devices into portable microscopes are also disclosed, thus providing the ability to identify bacteria on demand via motility analysis systems.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention belongs to the technical field of microbial culture, and discloses a method and application of facultative anaerobic microbial culture based on a microplate. Method: 1) Gellan gum is mixed with a culture medium, melted, and a suspension culture medium is obtained; the expanded cultured facultative anaerobic microorganisms are diluted with the suspension culture medium to obtain an indicator bacteria suspension; 2) A phosphate solution containing glucose is added to the outermost circle wells of the microplate, an indicator bacteria suspension is added to the inner wells, a glucose oxidase solution is added to the outermost circle wells, the plate is sealed, and the plate is statically cultured. The present invention solves the problem of reading errors caused by edge effects and bacterial sedimentation in traditional microplate culture, significantly improves data reliability, and reduces costs and operational complexity. The method of the present invention is used to detect the activity of antibacterial substances in facultative anaerobic bacteria.

The invention discloses an application of an active metabolite of marine actinomycetes HN2-56 in quorum sensing resistance. The active metabolites of the marine actinomycetes comprise propanediol tetraisopropyl alcohol ether, bisphenol A and 5-(2-methylphenyl)-4-pentenoic acid, the bisphenol A and the 5-(2-methylphenyl)-4-pentenoic acid have remarkable quorum sensing resisting activity, and the 5-(2-methylphenyl)-4-pentenoic acid has remarkable quorum sensing resisting activity on indicator bacterium chromobacterium violaceum. Compared with the prior art, the compound has no obvious growth inhibition on indicator bacteria and has anti-biofilm activity on the indicator bacteria staphylococcus aureus, and the propanediol tetraisopropyl alcohol ether is a prodrug of bisphenol A, so that the compound is expected to be applied to development of novel antibacterial substances taking a quorum sensing system as a target spot.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention relates to the technical field of seasoning preservation, in particular to a method for detecting the corrosion resistance of oyster sauce, which comprises the following steps: preparing a spore suspension by taking aspergillus niger ZE902 and aspergillus oryzae ZA306 as indicator bacteria; inoculating the spore suspension into a to-be-detected oyster sauce sample for culturing; in the culture process, observing whether the surface of the obtained culture is mildewed or not, sampling and detecting the viable count of indicator bacteria in the obtained culture, and judging the corrosion resistance of the oyster sauce sample to be detected according to the obtained observation result and the detected viable count of indicator bacteria. According to the method, the preservative capacity of the oyster sauce in the shelf life can be accurately judged, and the method is more representative than a traditional challenge experiment method.

The invention discloses a method for detecting common pollution indicator bacteria in a cell preparation, and belongs to the technical field of biological preparation detection. Compared with a direct inoculation method, a membrane filtration method and a fluorescent quantitative PCR method, the detection method provided by the invention has remarkable advantages, the detection time of the direct inoculation method and the membrane filtration method is 14d and does not meet the release detection standard of the cell preparation (less than 6h), the cost of the fluorescent quantitative PCR method is high, and the application technology of the method is not mature. According to the detection method, after the influence of the MSC is removed, the indicator bacterium genome is extracted for PCR verification, the detection result can be obtained only by 4-5 h in the whole operation process, the detection limit can be smaller than or equal to 100 CFU / mL, and the detection method has important application value for detecting common pollution indicator bacteria in the MSC preparation.

This invention discloses a method for preparing a strain of Lactobacillus saccharidus and its application in the preservation of chilled fresh meat, including the following steps: Step (1) Initial screening: Take 1.0 g of environmental sample, add 9 mL of sterile physiological saline, shake at 37℃ and 200 r / min for 30 min, sonicate for 10 min, then serially dilute to 10⁻⁴-10⁻⁶, take 100 μL and spread it on MRS solid medium, incubate at 37℃ for 48 h, pick single colonies and purify 3 times; Step (2) Antibacterial screening: Use 8 strains of putrefactive bacteria such as Enterobacter and Escherichia coli as indicator bacteria. The preparation method of this Lactobacillus saccharidus strain is based on natural sources, ensuring high safety and making it a suitable alternative to chemical preservatives. It exhibits significant inhibitory effects on major spoilage bacteria (such as Pseudomonas and Enterobacteriaceae) and pathogenic bacteria in chilled meat. Its low-temperature resistance (4℃) and acid and alkali resistance make it suitable for the storage environment of chilled meat. After application, it can reduce the total bacterial count, TVB-N content, pH value, and drip loss rate, delay color changes, and effectively extend the shelf life of chilled meat.

The invention relates to the technical field of microorganisms, and discloses a putrefying bacterium bacteriostasis determination method of a piperazine derivative, which comprises the following steps: S1, taking endophyte bacillus coagulans of cultured pseudosciaena crocea as a research object, obtaining 9 cyclic dipeptide small molecule compounds from 30L of fermentation liquor of the endophyte bacillus coagulans, the cyclic (proline-valine) has bacteriostatic activity, the cyclic (proline-valine) has bacteriostatic activity, and the cyclic (proline-valine) has bacteriostatic activity; performing structural modification screening by taking a piperazine ring as a parent nucleus; s2, screening a solvent, washing and activating the cultured three bacteria to obtain a bacterial suspension, and culturing and observing the bacterial suspension; s3, determining the maximum solubility of the compound by using a dynamic method; s4, completing a compound sensitivity experiment in a sterile environment; and S5, determining the minimum inhibitory concentration by using a broth dilution method. The research on the antibacterial structure-function relationship of the diketopiperazine compound further finds that the 1-Boc-4 (piperidine-4-yl)-piperazine and the 1-cyclopropyl methyl piperazine have good antibacterial activity on the three indicator bacteria, and have the potential value of being developed into preservatives and preservatives.

The application discloses an antibacterial peptide, a preparation method thereof and application of the antibacterial peptide in preparation of a preparation for treating pneumonia, and belongs to the technical field of biological medicines. The antibacterial peptide is a mutant of endolysin derived from a gram-negative bacteriophage. The bacteriophage endolysin plays an antibacterial / killing effect by degrading bacterial cell walls, and basically does not produce drug resistance. Common pneumonia pathogenic bacteria, i.e., Moraxella catarrhalis, Streptococcus pneumoniae and Staphylococcus aureus, are used as indicator bacteria, three antibacterial peptide mutants EAMP-M1, EAMP-M2 and EAMP-M3 with better antibacterial effects are screened, experiments prove that the three antibacterial peptide mutants have better antibacterial effects on gram-positive bacteria and gram-negative bacteria, can be applied to prevention, treatment or auxiliary antibiotic treatment of common pneumonia infection, and can be used in the field of medical instruments, such as sterilization of surgical instruments, and have a wide and good market application prospect.

This invention relates to a portable kit for rapid detection of microbial contamination of water, which includes two vials containing lactose broth culture medium and bromothymol blue color indicator, a dropper for sample collection, and an activatable heating pad. The main innovation of this invention is the use of an insulated box covered with a foil layer and, together with the heating pad, acting as an independent incubator. The vials are fixed inside a cylindrical foam and the sample, after addition, is placed at the appropriate growth temperature without the need for an incubator. This system allows for rapid and field detection of water-polluting bacteria, especially coliforms and Escherichia coli.

The application relates to the technical field of water body detection, in particular to a method for detecting acute toxicity of a water body by using surface-enhanced Raman scattering technology, which comprises the following steps: configuring an indicator bacterial suspension; providing a water body sample to be detected, carrying out first mixed culture of the indicator bacterial suspension and the water body sample to be detected, and separating and collecting a precipitate to obtain a first mixture; providing a probe solution, carrying out second mixed culture of the probe solution and the first mixture, and separating and collecting supernatant to obtain a mixture to be detected; providing a surface-enhanced Raman scattering substrate, mixing the mixture to be detected and the surface-enhanced Raman scattering substrate, using a Raman instrument to detect the characteristic peak intensity of a probe reduction product in the mixture to be detected, and calculating through an inhibition rate formula to analyze the toxicity of the water body. The method for evaluating the acute toxicity of the water body by combining SERS and a biosensor has good sensitivity, can rapidly detect and evaluate the acute toxicity problem of the water body in a short time.