20 results about "Indicator bacteria" patented technology

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present application relates to the technical field of animal health monitoring, and relates to a wild animal non-invasive genome monitoring and population health evaluation system. A time indication marker is generated based on the ratio of a predetermined indicator bacteria genus, and microenvironment data is input into a pre-trained microbial succession model to intelligently infer the actual exposure time of the sample in the wild. Then, the initial intestinal flora map of the sample at the instant of discharge is reconstructed. The high-fidelity flora map after reconstruction is further deeply coupled with health evaluation to realize scientific diagnosis and early warning of the physiological state of wild animal individuals. The accuracy of health evaluation, nutritional status analysis and pathogen detection is greatly improved, and major misjudgments such as misjudging environmental bacteria as pathogenic bacteria or misdiagnosing flora natural decay as ecological imbalance are avoided. A stable and reliable technical tool is provided for wild animal protection management.

The application discloses a royal jelly quality evaluation method based on bacteriostatic activity, and belongs to the technical field of bioengineering and food quality evaluation. The method evaluates the quality of royal jelly by determining the bacteriostatic activity of the royal jelly. Specifically, the royal jelly to be measured is diluted to 10% volume concentration, then the bacteriostatic activity of the diluted royal jelly on indicator bacteria is determined by adopting a filter paper method, and the quality grade of the royal jelly to be measured is determined according to the bacteriostatic ring diameter. The quality of the royal jelly is evaluated by determining the bacteriostatic activity of the royal jelly, the influence of water content, 10-HDA and freshness on the quality of the royal jelly is considered in principle, the method has the characteristics of simple operation and intuitive evaluation, and is easy to popularize and apply, thereby laying an important foundation for the quality detection technology of royal jelly.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention discloses a nisin titer detection method based on a microwell plate method. According to the growth inhibition effect of nisin on indicator bacteria, a plant lactobacillus L30 with a good strain growth amount-nisin titer curve is screened from existing strains, the plant lactobacillus L30 is applied to a microwell plate method, and a nisin titer determination method which is high in accuracy, convenient and economical is developed.

Loop-mediated isothermal amplification assays, kits, and methods that target and / or detect the presence of a fecal indicator bacteria in a sample. These assays, kits, and methods can be portable and capable of providing fast (within 60 minutes) results in the field, eliminating the need for a laboratory and other complex equipment.

The invention discloses an antibacterial peptide as well as a preparation method and application thereof in preparation of a preparation for treating pneumonia, and belongs to the technical field of biological medicines. The antibacterial peptide is a mutant of endolysin derived from gram negative bacteriophage, and the endolysin of the bacteriophage plays a bacteriostatic / bactericidal role by degrading bacterial cell walls, and basically does not generate drug resistance. Common pneumonia pathogenic bacteria including moraxella catarrhalis, streptococcus pneumoniae and staphylococcus aureus are used as indicator bacteria, three antibacterial peptide mutants EAMP-M1, EAMP-M2 and EAMP-M3 with good antibacterial effects are obtained through screening, and experiments prove that the antibacterial peptide mutants have good antibacterial effects on gram-positive bacteria and gram-negative bacteria; the composition can be applied to prevention, treatment or auxiliary antibiotic treatment of common pneumonia infection; and the disinfectant is used in the field of medical instruments, such as surgical instrument disinfection, and has a wide and good market application prospect.

The invention relates to bacillus siamensis and an application of the bacillus siamensis in aquaculture. The bacillus siamensis is obtained by carrying out antagonistic strain separation and screening by taking vibrio parahaemolyticus as an indicator bacterium. Through verification, the strain has a relatively obvious inhibition effect on vibrio parahaemolyticus causing vibriosis in aquaculture, also has certain resistance to pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, in addition, the strain has relatively good high-temperature resistance, acid and alkali resistance and salt tolerance, and when the strain or a microbial agent prepared from the strain is applied to aquaculture, the yield of aquaculture is greatly increased. The compound has high inhibition rate on various germs including vibrio parahaemolyticus in a water body, does not influence the water body environment, has potential effects of promoting the growth of bred animals, stimulating the response of an immune system, improving the immunity, improving the water quality condition, repairing the ecological environment and the like, and has a wide application prospect.

The invention discloses trichoderma for efficiently preventing and controlling tobacco root rot and a culture method thereof, which are characterized in that tobacco root rot biocontrol bacteria are separated from tobacco rhizosphere soil, tobacco root rot pathogenic bacteria share fusarium F. Commune as indicator bacteria, and bacterial strains with bacteriostatic action on the growth of fusarium solani are screened to obtain the trichoderma LB8-12-2; the method can effectively inhibit the tobacco from being infected by fusarium pathogenic bacteria in the growth process, and can greatly improve the survival rate and quality of the tobacco in the growth process when being applied to the tobacco growth process. Experimental data show that after tobacco is treated by the trichoderma LB8-12-2, the relative control effect is 95.51%, and it is indicated that the trichoderma LB8-12-2 has a good control effect on the tobacco fusarium root rot, so that the trichoderma LB8-12-2 has remarkable advantages in the aspects of protecting the environment, reducing pesticide residues and improving the safety of tobacco leaves when being used for controlling the tobacco fusarium root rot, and the application prospect is wide.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention belongs to the technical field of microbial culture, and discloses a method and application of facultative anaerobic microbial culture based on a microplate. Method: 1) Gellan gum is mixed with a culture medium, melted, and a suspension culture medium is obtained; the expanded cultured facultative anaerobic microorganisms are diluted with the suspension culture medium to obtain an indicator bacteria suspension; 2) A phosphate solution containing glucose is added to the outermost circle wells of the microplate, an indicator bacteria suspension is added to the inner wells, a glucose oxidase solution is added to the outermost circle wells, the plate is sealed, and the plate is statically cultured. The present invention solves the problem of reading errors caused by edge effects and bacterial sedimentation in traditional microplate culture, significantly improves data reliability, and reduces costs and operational complexity. The method of the present invention is used to detect the activity of antibacterial substances in facultative anaerobic bacteria.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

This invention discloses a method for preparing a strain of Lactobacillus saccharidus and its application in the preservation of chilled fresh meat, including the following steps: Step (1) Initial screening: Take 1.0 g of environmental sample, add 9 mL of sterile physiological saline, shake at 37℃ and 200 r / min for 30 min, sonicate for 10 min, then serially dilute to 10⁻⁴-10⁻⁶, take 100 μL and spread it on MRS solid medium, incubate at 37℃ for 48 h, pick single colonies and purify 3 times; Step (2) Antibacterial screening: Use 8 strains of putrefactive bacteria such as Enterobacter and Escherichia coli as indicator bacteria. The preparation method of this Lactobacillus saccharidus strain is based on natural sources, ensuring high safety and making it a suitable alternative to chemical preservatives. It exhibits significant inhibitory effects on major spoilage bacteria (such as Pseudomonas and Enterobacteriaceae) and pathogenic bacteria in chilled meat. Its low-temperature resistance (4℃) and acid and alkali resistance make it suitable for the storage environment of chilled meat. After application, it can reduce the total bacterial count, TVB-N content, pH value, and drip loss rate, delay color changes, and effectively extend the shelf life of chilled meat.

The application discloses an antibacterial peptide, a preparation method thereof and application of the antibacterial peptide in preparation of a preparation for treating pneumonia, and belongs to the technical field of biological medicines. The antibacterial peptide is a mutant of endolysin derived from a gram-negative bacteriophage. The bacteriophage endolysin plays an antibacterial / killing effect by degrading bacterial cell walls, and basically does not produce drug resistance. Common pneumonia pathogenic bacteria, i.e., Moraxella catarrhalis, Streptococcus pneumoniae and Staphylococcus aureus, are used as indicator bacteria, three antibacterial peptide mutants EAMP-M1, EAMP-M2 and EAMP-M3 with better antibacterial effects are screened, experiments prove that the three antibacterial peptide mutants have better antibacterial effects on gram-positive bacteria and gram-negative bacteria, can be applied to prevention, treatment or auxiliary antibiotic treatment of common pneumonia infection, and can be used in the field of medical instruments, such as sterilization of surgical instruments, and have a wide and good market application prospect.

This invention relates to a portable kit for rapid detection of microbial contamination of water, which includes two vials containing lactose broth culture medium and bromothymol blue color indicator, a dropper for sample collection, and an activatable heating pad. The main innovation of this invention is the use of an insulated box covered with a foil layer and, together with the heating pad, acting as an independent incubator. The vials are fixed inside a cylindrical foam and the sample, after addition, is placed at the appropriate growth temperature without the need for an incubator. This system allows for rapid and field detection of water-polluting bacteria, especially coliforms and Escherichia coli.

The application relates to the technical field of water body detection, in particular to a method for detecting acute toxicity of a water body by using surface-enhanced Raman scattering technology, which comprises the following steps: configuring an indicator bacterial suspension; providing a water body sample to be detected, carrying out first mixed culture of the indicator bacterial suspension and the water body sample to be detected, and separating and collecting a precipitate to obtain a first mixture; providing a probe solution, carrying out second mixed culture of the probe solution and the first mixture, and separating and collecting supernatant to obtain a mixture to be detected; providing a surface-enhanced Raman scattering substrate, mixing the mixture to be detected and the surface-enhanced Raman scattering substrate, using a Raman instrument to detect the characteristic peak intensity of a probe reduction product in the mixture to be detected, and calculating through an inhibition rate formula to analyze the toxicity of the water body. The method for evaluating the acute toxicity of the water body by combining SERS and a biosensor has good sensitivity, can rapidly detect and evaluate the acute toxicity problem of the water body in a short time.