Method for quick combinative monitoring of total coliform and escherichia coli

A technology for Escherichia coli and Escherichia coli, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and determination/inspection of microorganisms. At the same time, problems such as joint monitoring and inability to quantitatively detect can achieve the effects of reducing monitoring time, high detection rate and simple method.

Inactive Publication Date: 2016-08-31
CHONGQING THREE GORGES UNIV
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Problems solved by technology

However, traditional culture methods such as multi-tube fermentation method and membrane filtration method have many steps, are cumbersome, time-consuming (at least 3 days), and cannot simultaneously monitor two or more indicator bacteria.
Although the PCR method has the characteristics of sensitive and rapid detection of bacteria, it cannot quantitatively detect a variety of living bacteria in water.
Therefore, the PCR method is not currently used for the monitoring of total coliforms and Escherichia coli as indicators of daily water quality fecal pollution

Method used

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  • Method for quick combinative monitoring of total coliform and escherichia coli
  • Method for quick combinative monitoring of total coliform and escherichia coli
  • Method for quick combinative monitoring of total coliform and escherichia coli

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Embodiment 1

[0021] During the three-year period from October 2009 to September 2012, 200 water samples were collected from 5 monitoring points in the Wanzhou section of the Three Gorges Reservoir. The five monitoring points include a water intake point of a water plant, the upper reaches of the city, the lower reaches of the city, and the backwater areas of two tributaries. From October 2009 to September 2012, water samples were collected 1-2 times a month from the monitoring point with a water collector, and the water samples were placed in a 4°C dark box in a sterile plastic bottle and immediately transported back to the laboratory. This method and the national standard are used. Multi-tube fermentation method for detection. The collected water samples were diluted tenfold, from 10 0 to 10 -4 10 out of five dilutions 0 , 10 -1 , 10 -2 or 10 -1 , 10 -2 , 10 -3Three concentrations, 1mL water samples were inoculated into five test tubes containing pre-medium for each concentration ...

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Abstract

The invention provides a method for quick combinative monitoring of total coliform and escherichia coli. Water fecal pollution indicator bacteria including total coliform and escherichia coli are defected through the most probable number duplex PCR (MPN-dulpex PCR) method. The method is simple and quick, only four steps of experiments are needed, and the detection rate is higher than that of the national standard multi-tube fermentation method; specificity is high, repeatability is good, and two alive indicator bacteria including total coliform and escherichia coli in water can be monitored at the same time in a combinative and quantitative mode. A new method is provided for quick combinative monitoring of the water fecal pollution indicator bacteria including total coliform and escherichia coli.

Description

technical field [0001] The invention relates to a detection method for bacteria, in particular to a method for rapidly and jointly monitoring total coliforms and Escherichia coli which are indicators of water quality feces pollution. Background technique [0002] Fecal contamination of water quality can easily lead to outbreaks of waterborne infectious diseases. Due to the fact that there may be hundreds of waterborne pathogens in water, the concentration of pathogens is usually low, and the detection methods are relatively cumbersome, time-consuming, requiring professionals, equipment and biosafety laboratories, etc., routine monitoring of pathogens is in most countries, especially developing countries. It is difficult to promote and apply it in China. It is mainly to monitor the indicator bacteria of fecal pollution to indirectly assess the pollution risk of water quality pathogens. In fact, thousands of water quality assessments are conducted around the world each day ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/06C12R1/19
CPCC12Q1/6851C12Q1/689C12Q2600/158C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 肖国生
Owner CHONGQING THREE GORGES UNIV
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