225results about How to "Reduce experimental steps" patented technology

The invention provides a method for preparing silicon dioxide-coated quantum dots. The method comprises the following steps of: dispersing CdSe/CdS core-shell structural quantum dots into cyclohexane to obtain first mixed liquid; adding n-hexane, surfactant and ethyl orthosilicate into the first mixed liquid to obtain second mixed liquid; adding ammonia water into the second mixed liquid to carry out reaction; and depositing and separating a reaction product to obtain the silicon dioxide-coated quantum dots. The method firstly disperses the CdSe/CdS core-shell structural quantum dots into the cyclohexane to obtain the first mixed liquid, and then adds the n-hexane into the first mixed liquid serving as a dispersant. Since the n-hexane has similar polarity to the cyclohexane, the quantum dots can be dispersed into the n-hexane well. SiO2 formed by the hydrolysis of the ethyl orthosilicate can be coated on the quantum dots to the largest extent to obtain SiO2-coated CdSe/CdS core-shell structural quantum dots with uniform particle size. Compared with the prior art, the method for preparing the silicon dioxide-coated quantum dots has the advantages of few experimental procedures, simple operation and easy control.

The invention provides a micro-fluidic chip based on a magnetic fluorescence composite particle. The micro-fluidic chip comprises a chip bottom-layer plate, a chip channel layer and a chip upper-layer cover plate which are sequentially arranged from bottom to top, wherein the chip channel layer comprises a sample introduction area, a sample introduction channel, an optical detection area, a waste liquid channel and a waste liquid storage area which are sequentially connected; a capture recognition reagent is fixed to the lower side of the optical detection area; mark recognition reagent-magnetic fluorescence composite particles are pre-fixed on the inner upper sides of the sample introduction area, the sample introduction channel or the optical detection area. The invention also provides an optical detection device. The micro-fluidic chip provided by the invention can be used for sample trace substance analysis through magnetism enrichment and separation reaction and a fluorescence optical detection technology, meets the requirements on quick, sensitive and convenient detection of low-concentration substances in a micro-sample, solves the problems of complicated and expensive instrument equipment, inconvenience, slow detection speed and the like of an existing method, and has a favorable application value in the POCT (Point-of-Care Testing) detection field.

The invention discloses a method for preparing a flexible self-supporting graphene film doped with metal nanoparticles. Firstly, the graphene and the metal salt solution are mixed, and the mixed solution is titrated on a solid substrate and dried to form a film. The obtained film is put into ascorbic acid aqueous solution and heated for reduction to obtain a flexible self-supporting graphene film doped with metal nanoparticles inside. The graphene oxide aqueous solution was firstly titrated on a solid substrate and dried to form a film. Further, the metal salt solution is titrated on the graphene oxide film, and after drying, it is placed in an aqueous solution of ascorbic acid for heating and reduction to obtain a flexible self-supporting graphene film doped with metal nanoparticles on the surface. The method has simple preparation, few reaction steps, convenient operation, simple equipment and process requirements, cheap raw materials, and low cost, and can realize the preparation of graphene films doped with metal nanoparticles inside and graphene films doped with metal nanoparticles on the surface .

The invention discloses a molecular design and preparation method of a nitric oxide fluorescent probe based on a double-response mechanism. The structure of the nitric oxide fluorescent probe can be represented by a general formula (I) (please see the formula in the description), wherein Spacer represents a single bond or acetylene bond or thiophene or substituted aryl unit, Ar represents one of an aryl group, a substituted aryl group, a heterocyclic aryl group and substituted aryl amine, and the substituted group can be one of a hydrophilic ether chain, a sulfonic acid group, quaternary ammonium salt and the like. The key steps of synthesis of the probe molecule can be achieved through a Suzuki coupling reaction or a C-H activation coupling reaction and the like. The probe molecule has the advantages of being high in sensitivity, good in selectivity, short in response time, good in membrane permeability, low in background noise, capable of achieving direct observation and fluorescence-ultraviolet-visible spectrum dual-band response and the like, is a brand-new sensing detection platform and has a good application prospect.

The invention relates to a carriage window air-flow state tracking device and experimental method. The method comprises the steps: a fan is turned on to circulate the air in a pipeline, a flue gas generator is turned on, the flue gas is released through a main pipe, a branch pipe, a flow regulating value, a buffer tube, a release tube and a releasing hole to carriage window, and the rotating speed of the fan is regulated to control the flow, so that the trace of the flue gas in the carriage is clearly visible; a camera shoots the released flue gas and saved the image to a computer, real-time flow state of the air in the carriage is directly displayed, and the wind direction at the window is judged; wind speed data collected by a wind-velocity indicator is recorded by a data collector and introduced into the computer, the entrance time and speed curves are drawn by the data collector, the ventilation volume at the carriage window is calculated hereby, so that the data is provided for wind field simulation and fire oxygen consumption calculation of following fire space, and the device and the method are important for fire state judgment in fire-fighting study.

The invention discloses a dynamic pressure regulating gas absorption and desorption experiment device based on a high-pressure volumetric method. The dynamic pressure regulating gas absorption and desorption experiment device comprises a dynamic pressure regulating inflation system, a vacuum degassing system, a gas absorption and desorption system, a constant-temperature system, a metering systemand a data processing system, wherein the dynamic pressure regulating inflation system is provided with a high-pressure gas cylinder, a pressure regulating valve, a high-pressure valve, a pressure gauge, a dynamic pressure regulating inflation tank and a three-way connector; the vacuum degassing system is provided with a vacuum pump, a motor, a vacuum diaphragm valve, a vacuum gauge tube, a vacuumgauge, a vacuum tank path, a high-pressure valve and a three-way connector; the gas absorption and desorption system is provided with an absorption and desorption tank, a pressure gauge and a high-pressure valve; the constant-temperature system is provided with a super constant-temperature water bath tank; the metering system is provided with a flow meter, a leveling bottle, a measuring cylinder,a glass piston, a sampling bag and a lifter; the data processing system is provided with a pressure sensor, a data collector, a computer and a printer. The dynamic pressure regulating gas absorptionand desorption experiment device disclosed by the invention has the advantages of convenience in operation, high experiment accuracy, accurate results and ability in achieving gas absorption and desorption experiments.

The invention discloses a heat accumulation capsule for decreasing the temperature of main stream smoke of cigarettes. The heat accumulation capsule comprises a wall material and a core material, wherein the core material contains an inorganic phase-change material; and the wall material is polylactic acid; and the inorganic phase-change material is disodium hydrogen phosphate dodecahydrate. The invention further discloses a preparation method of the heat accumulation capsule. The preparation method comprises the steps of simultaneously dropwise adding a core material solution and a wall material solution into concretion liquid respectively through inner holes of an electrostatic spinning coaxial needle head and gaps between inner and outer holes of the electrostatic spinning coaxial needle head, stirring, and carrying out solid-liquid separation, washing, cooling and concretion. The preparation method is different from existing commonly adopted chemical methods, and the inorganic phase-change material is coated by virtue of a physical method. Compared with the chemical methods, the preparation method has the advantages that the complicated chemical reaction steps are reduced, theovermuch chemical solvent is not used, a device is simple, and the particle size of the heat accumulation capsule is controllable.

The invention relates to a method for preparing a SiBCN-containing thermally-polymerisable ceramic precursor. The method comprises the steps of enabling vinyl dichlorosilane and hexamethyldisilazane, which are in a certain ratio, to react at a certain temperature, adding a certain amount of NaBH4 reducer into the reaction product for reduction after reaction is complete, then, adding dimethyl vinyl chlorosilane into the reduced reaction product to continue reaction, heating up the reaction product to distill off a solvent and some unreacted raw material molecules, finally, adding a solvent n-hexane into the reaction product to wash the reaction product, thereby obtaining a colorless viscous liquid product, i.e., the soluble and fusible SiBCN ceramic precursor. According to the synthesis method, the operation is simple and convenient, experimental conditions are easily controlled, experimental steps are reduced, the cost is low, the prepared SiBCN ceramic precursor is soluble and fusible, has good processability, is good in fluidity and can be subjected to thermal polymerization directly, the yield of the prepared product can reach 60% the input amount of chlorosilane, and the yield of sintered ceramics can reach 64%.

The invention relates to a simple measurement device for the heat conductivity coefficient of porous ceramics. The device comprises a heater arranged at the bottom, wherein the top of the heater is provided with a heat source, the top of the heat source is provided with an upper sheet copper and a lower sheet copper which correspond to each other up and down, the lower sheet copper is directly contacted with the heat source to be taken as a hot end, and the upper sheet copper is taken as a cold end; when the heat conductivity coefficient is measured, the porous ceramics to be measured is arranged between the upper sheet copper and the lower sheet copper; the upper sheet copper and the lower sheet copper are respectively connected with a thermoelectric couple; and a heat preservation cover is packed on the upper sheet copper, the porous ceramics to be measured and the lower sheet copper, and the top of the outer side of the heat preservation cover is provided with a counter weight block. The simple measurement device is used for computing the heat conductivity coefficient by adopting the Fourier heat transfer law, and is simple in structure; parts used for building the device, i.e. the heater, the heat source, the sheet coppers, the thermoelectric couples and the like, are not expensive, so that the simple measurement device is low in cost; and the experimental the number of operation steps for measuring the heat conductivity coefficient by using the device is small, and compared with the measurement by using a steady-state method which needs a plurality of hours for once, the measurement by using the device commonly needs 10-20min for once.

The invention discloses a method for eliminating a digital holographic zero-order image through one-step arbitrary angle phase shift. A linkage coaxial dual-reference-light light path is provided for the first time; by adjusting a beam splitting crystal loaded to an inching translation platform in the light path, the phase shift magnitudes of two beams of reference light are guaranteed to be -alpha and 2alpha when the inching translation platform displaces. Based on the light path, a matched algorithm is provided, the existing phase shift zero-order digital holographic elimination technology is changed into one-step phase shift from multiple steps of phase shift, the phase shift magnitude is arbitrary, and the error produced by multi-step phase shift and movement of the inching translation platform is eliminated, so the phase shift technology is easier and more convenient and the operability is higher.

The invention discloses a micro-fluidic chip for extracting total nucleic acid of bacteria in human whole blood. The chip comprises a positioning hole and a main body. The main body comprises a sampling hole formed in a sampling cavity, the sampling cavity, a one-way valve, a nucleic acid extraction material, a nucleic acid extraction cavity, a PCR reaction cavity, a buffer solution I cavity, a buffer solution II cavity, an RNA enzyme inhibitor cavity, an MIX1 cavity, a DNase I cavity, a MIX2 cavity, a 70% ethanol cavity, a buffer solution II cavity, an MIX3 cavity, a PCR Master Mix cavity, aPCR primer cavity, a PCR reaction probe cavity and a buffer solution V cavity. The chip provided by the invention can split the bacteria quickly to release RNA, and is simple and convenient to operate, so that the extraction efficiency is improved greatly; the detection limit is reduced to the lowest, so that the probability of leaked detection is avoided; the operating process of pathogenic bacteria detection is simplified, and the detection error caused by manual operations is reduced.

The invention discloses a vanadium trichloride mixed powder and the application thereof in quickly measuring the nitrate content in the food. The vanadium trichloride mixed powder is composed of sodium chloride, sulfamic acid, sulfanilic acid, naphthyl ethylenediamine hydrochloride and vanadium trichloride. When the vanadium trichloride mixed powder is applied to quickly measure the nitrate content in the food, firstly, the food is homogenized and then metered in volume by using the acetic acid solution. After that, the homogenized food is oscillated and filtered to obtain a sample solution. Secondly, the vanadium trichloride mixed powder is added into the sample solution, and the obtained product is shaken uniformly, subjected to water bath and cooled. The light absorption value of the obtained product is measured and then the nitrate content is calculated according to a standard curve. According to the technical scheme of the invention, the method is simple and easy to operate, fast, short in consumed time, sensitive, accurate, small in reagent consumption, and low in cost. Meanwhile, an operator can master the method without being specially trained. Moreover, reagents adopted in the method are not volatilized, free of odors and extremely small in toxicity to human bodies. The mixed power is good in stability. The detection reagent is harmless to the environment and human bodies. Therefore, the vanadium trichloride mixed powder has a wide application prospect in detecting the nitrate content in the rapid detection field of food especially the rapid detection field of fresh vegetables.

The invention relates to a child-friendly implicit attitude test method and device. The child-friendly implicit attitude test method comprises: step S1, a touch display screen randomly displays a target stimulus target at the center, and respectively displays a positive attribution key and a negative attribution key at the lower left corner and the lower right corner; step S2, the reaction time and the correctness that a person to be tested executes a compatible mission are recorded; step S3, the step S2 is executed repeatedly until the compatible mission is executed for M times; step S4, the reaction time and the correctness that the person to be tested execute an incompatible mission; step S5, the step S4 is executed repeatedly until the incompatible mission is executed for N times; step S6, an implicit attitude index is calculated. The child-friendly implicit attitude test method and device provide tool supports for the related fields including development of implicit attitudes and explicit attitudes, and implicit attitudes and infant behaviors, and can make up of the defect that a child implicit attitude measurement tool is lacked at home and abroad; and the attitude test method and device are convenient and practical, have a friendly interface and are simple to operate.

The invention relates to an integrated printing device and printing method of a continuous fiber embedded material, and belongs to the technical field of function material 3D printing. A polymer cylinder filamentation stick with a hole is preformed, and a continuous fiber penetrates through the hole of the filamentation stick; the filamentation stick is tightly clamped in a filamentation barrel; apush rod pushes the filamentation stick downwards; the filamentation stick passes through a heating area; molten polymer flows downwards to wrap the surface of the continuous fiber; finally, a formedwire comes out from the bottom of the filamentation barrel; the formed wire passes through a measuring and shearing mechanism, is shorn when the diameter does not meet the requirement and directly enters a feeding mechanism when the diameter meets the requirement; the feeding mechanism unceasingly feeds the formed wire downwards; and the formed wire is fed into a printing head part, and printingis achieved. The integrated printing device and printing method have the advantages that adhesiveness between the fiber and the polymer is good, local accumulation of the continuous fiber cannot be generated, formed wires of different diameters can be obtained through printing according to requirements, and printing of continuous fiber embedded type high-melting-point polymer is achieved.

The invention discloses a staining method of ciliates, belongs to the technical field of biology, and provides a staining method of ciliates to solve the problems that a traditional ciliate staining method is long in staining time and fussy in staining step, and cytostome and cilium cannot be clearly displayed. A saturated ethyl alcohol mercuric chloride solution is adopted as a fixing agent to carry out pre-fixation on ciliate bodies. In addition, before the pre-fixation operation, to-be-produced ciliate bodies are attached to a to-be-dyed glass slide; a plurality of steps such as dropwise adding protein glycerinum water and collodion are omitted. The staining method disclosed by the invention is convenient to operate; the sensitivity and the staining effect of silver staining of the ciliate protein are greatly improved; and rapid, efficient and accurate display of infraciliature in morphological classification and cytological studies of the ciliates is achieved.

The invention discloses a primer group, a kit and a method for detecting hypertension drug related gene polymorphism. The primer group is selected from SEQ ID NO.1-SEQ ID NO.360 nucleotide sequences.The primer group can directly perform PCR after multiple PCR capturing; the primer group and the kit are simple to operate, and experiment steps and check are reduced; besides, consumable and labor cost for construction of a DNA library can be further reduced; drug kinds guided by gene sites corresponding to 180 pairs of primers comprise fix common hypertension treatment drugs and hypertension complication drugs including anti-freezing, anti-platelet, sugar-reducing, aldosterone and statin common drugs, selection of group treatment schemes for complications such as newly diagnosed hypertension, H type hypertension, refractory hypertension and hypertension atherosclerosis can be guided, so that patients can be effectively treated as early as possible.