225results about How to "Reduce experimental steps" patented technology

The invention provides a method for preparing silicon dioxide-coated quantum dots. The method comprises the following steps of: dispersing CdSe / CdS core-shell structural quantum dots into cyclohexane to obtain first mixed liquid; adding n-hexane, surfactant and ethyl orthosilicate into the first mixed liquid to obtain second mixed liquid; adding ammonia water into the second mixed liquid to carry out reaction; and depositing and separating a reaction product to obtain the silicon dioxide-coated quantum dots. The method firstly disperses the CdSe / CdS core-shell structural quantum dots into the cyclohexane to obtain the first mixed liquid, and then adds the n-hexane into the first mixed liquid serving as a dispersant. Since the n-hexane has similar polarity to the cyclohexane, the quantum dots can be dispersed into the n-hexane well. SiO2 formed by the hydrolysis of the ethyl orthosilicate can be coated on the quantum dots to the largest extent to obtain SiO2-coated CdSe / CdS core-shell structural quantum dots with uniform particle size. Compared with the prior art, the method for preparing the silicon dioxide-coated quantum dots has the advantages of few experimental procedures, simple operation and easy control.

The invention provides a new Mueller matrix measurement system and method, and can measure a Mueller matrix of a target efficiently and accurately. The measurement system comprises a light source system, a polarization system, a to-be-measured target, a polarization detection system and a computer processing system which are arranged successively along an optical path. The polarization system comprises a vertical linear polarizing film, a first liquid crystal phase retarder and a second liquid crystal phase retarder arranged successively along an incident optical path; included angles of fastaxes of two liquid crystal phase retarders with the horizontal direction are 45 DEG and 90 DEG respectively. The polarization detection system is a polarization camera for simultaneously detection ofdivided aperture full polarization states. The light source system meets the requirements: polarized light emitted by the to-be-measured target eventually covers all the optical channels of the polarization camera; the computer processing system is used for controlling the voltage loaded onto the two liquid crystal phase retarders to modulate the polarization state of the incident light on the to-be-measured target, and storing an intensity map collected by the camera, and calculating the Muller matrix according to the intensity value.

The invention provides a pretreatment method for measuring multiresidues of pesticides in environmental biology. The method comprises the steps of mixing environmental biology earthworms with water to obtain an earthworm-water mixed solution; conducting ultrasound mixing on the earthworm-water mixed solution and acetonitrile, afterwards conducting homogenating, mixing a homogenating sizing agent with inorganic salts, and conducting vibrating and solid-liquid separation to obtain an acetonitrile extraction solution; mixing the acetonitrile extraction solution with Fe3O4-SiO2 magnetic particles, ZrO2, a PSA absorbent and a water absorbent to obtain mixed feed liquor; vibrating the mixed feed liquor, conducting magnetic separation and filtering, and then collecting clean liquor. According to the pretreatment method for measuring multiresidues of the pesticides in the environmental biology, aiming at the characteristics that an earthworm matrix is high in fat and protein, ZrO2 and PSA absorbent are selected as a purifying material, and based on the superparamagnetism advantage of a magnetic nano material Fe3O4-SiO2 and the selectivity of the magnetic nano material Fe3O4-SiO2, the pretreatment method for quickly measuring the multiresidues of pesticides in the environmental biology is built, and thus quick and precise measurement of multiresidues of the pesticides in the environmental biology is achieved.

The invention provides a micro-fluidic chip based on a magnetic fluorescence composite particle. The micro-fluidic chip comprises a chip bottom-layer plate, a chip channel layer and a chip upper-layer cover plate which are sequentially arranged from bottom to top, wherein the chip channel layer comprises a sample introduction area, a sample introduction channel, an optical detection area, a waste liquid channel and a waste liquid storage area which are sequentially connected; a capture recognition reagent is fixed to the lower side of the optical detection area; mark recognition reagent-magnetic fluorescence composite particles are pre-fixed on the inner upper sides of the sample introduction area, the sample introduction channel or the optical detection area. The invention also provides an optical detection device. The micro-fluidic chip provided by the invention can be used for sample trace substance analysis through magnetism enrichment and separation reaction and a fluorescence optical detection technology, meets the requirements on quick, sensitive and convenient detection of low-concentration substances in a micro-sample, solves the problems of complicated and expensive instrument equipment, inconvenience, slow detection speed and the like of an existing method, and has a favorable application value in the POCT (Point-of-Care Testing) detection field.

The invention discloses a flexible self-support graphene conductive thin film with a microstructure pattern on the surface and a preparation method of the flexible self-support graphene conductive thin film. The preparation method comprises the following steps of filling a cellulose nitrate solution in a female template with the microstructure patter on the surface, drying and stripping the female template to obtain a cellulose nitrate thin film with a reverse microstructure as a substrate; carrying out plasma surface processing on the substrate; filling a graphene oxide solution in the substrate and drying graphene oxide solution to form a film; placing the obtained graphene oxide thin film in hydroiodic acid aqueous solution for heating and reduction; and removing the cellulose nitrate thin film to obtain the flexible self-support graphene conductive thin film with the same microstructure pattern as the female template. The method has the advantages of simplicity in preparation, convenience in operation, simplicity in device and process requirements, cheap raw material and low cost, and industrial production at a large scale can be achieved. The prepared self-support graphene film is high in toughness and high in conductivity, the microstructure pattern on the surface is adjustable, and the self-support graphene film is expected to be used for aspects such as flexible electronics, flexible display, a wearable sensing device and an energy storage device.

The invention discloses a method for preparing a flexible self-supporting graphene film doped with metal nanoparticles. Firstly, the graphene and the metal salt solution are mixed, and the mixed solution is titrated on a solid substrate and dried to form a film. The obtained film is put into ascorbic acid aqueous solution and heated for reduction to obtain a flexible self-supporting graphene film doped with metal nanoparticles inside. The graphene oxide aqueous solution was firstly titrated on a solid substrate and dried to form a film. Further, the metal salt solution is titrated on the graphene oxide film, and after drying, it is placed in an aqueous solution of ascorbic acid for heating and reduction to obtain a flexible self-supporting graphene film doped with metal nanoparticles on the surface. The method has simple preparation, few reaction steps, convenient operation, simple equipment and process requirements, cheap raw materials, and low cost, and can realize the preparation of graphene films doped with metal nanoparticles inside and graphene films doped with metal nanoparticles on the surface .

The invention discloses a molecular design and preparation method of a nitric oxide fluorescent probe based on a double-response mechanism. The structure of the nitric oxide fluorescent probe can be represented by a general formula (I) (please see the formula in the description), wherein Spacer represents a single bond or acetylene bond or thiophene or substituted aryl unit, Ar represents one of an aryl group, a substituted aryl group, a heterocyclic aryl group and substituted aryl amine, and the substituted group can be one of a hydrophilic ether chain, a sulfonic acid group, quaternary ammonium salt and the like. The key steps of synthesis of the probe molecule can be achieved through a Suzuki coupling reaction or a C-H activation coupling reaction and the like. The probe molecule has the advantages of being high in sensitivity, good in selectivity, short in response time, good in membrane permeability, low in background noise, capable of achieving direct observation and fluorescence-ultraviolet-visible spectrum dual-band response and the like, is a brand-new sensing detection platform and has a good application prospect.

The invention discloses a dry-powder isothermal amplification detection reagent capable of being transported at normal temperature, and a preparation method of the detection reagent. The detection reagent can be prepared by the following steps: adding a freeze-drying protective additive into a liquid reagent, and adopting a vacuum freeze drying method to freeze-dry components of the liquid isothermal amplification detection reagent to be solid dry powder. The solid reagent can be transported at normal temperature; during reaction, all that is needed is to add a liquid reaction substrate to be dissolved; the reactivity is excellent, the operation is convenient, and the dry-powder isothermal amplification detection reagent can be widely used in on-site quick detection of various pathogenes, food microorganisms, transgenic products and the like.

The invention relates to a carriage window air-flow state tracking device and experimental method. The method comprises the steps: a fan is turned on to circulate the air in a pipeline, a flue gas generator is turned on, the flue gas is released through a main pipe, a branch pipe, a flow regulating value, a buffer tube, a release tube and a releasing hole to carriage window, and the rotating speed of the fan is regulated to control the flow, so that the trace of the flue gas in the carriage is clearly visible; a camera shoots the released flue gas and saved the image to a computer, real-time flow state of the air in the carriage is directly displayed, and the wind direction at the window is judged; wind speed data collected by a wind-velocity indicator is recorded by a data collector and introduced into the computer, the entrance time and speed curves are drawn by the data collector, the ventilation volume at the carriage window is calculated hereby, so that the data is provided for wind field simulation and fire oxygen consumption calculation of following fire space, and the device and the method are important for fire state judgment in fire-fighting study.

The invention belongs to the technical field of pesticide and midbody synthesis, particularly relates to a synthesis method for bifenazate. In the synthesis method for the bifenazate, a target product is synthetized by taking para-hydroxydiphenyl as a raw material through nitration, methylation, hydrogenated reduction, diazotization, diazonium salt reduction and an acylation one-step method, wherein acylation reaction is directly performed on hydrazonium salt without treatment, thus, the experimental procedure is shortened, the yield is improved to above 65 percent from 50 percent, the conversion rate of a reaction product is high, and the purification treatment is easy to perform. The synthesis method for the bifenazate has the advantages that the operation is simple, the condition is mild, the yield is high, the cost is low, and the like, is easy to carry out industrial production and has a wide application prospect. The bifenazate synthetized by the invention can be used as a specific acaricide, is active in active stage of tetranychid, can also control pests, such as Tetranychus urticae, in the egg stage, and is used for cotton, hop and certain fruiter crops to effectively prevent all mites in the growth stage.

The invention discloses an LIBS multi-component quantitative inversion method based on a deep learning convolutional neural network, and is suitable for the field of laser spectrum analysis. Accordingto the method, the unique advantages of the convolutional neural network algorithm in the aspect of image feature recognition are utilized, and the method is applied to LIBS spectrum quantitative inversion. According to the convolutional neural network construction scheme designed by the invention, feature extraction and deep learning can be performed on the LIBS spectral line form of the sample,and after the convolutional neural network is trained by using the LIBS spectrum of the known sample, the network can analyze and predict the contents of various chemical components of the unknown sample at the same time. The method has the advantages of being simple and convenient to operate, efficient in training, high in accuracy and good in robustness, is suitable for quantitatively analyzingthe LIBS spectrum, and is particularly suitable for analyzing the LIBS spectrum with relatively high spectral line form complexity and relatively large interference noise.

The invention discloses a production method of a metal nano-particle doped flexible self-supporting graphene film. The method comprises the following steps: mixing graphene with a metal salt solution, titrating the obtained mixed solution on a solid substrate, and drying the mixed solution to form a film; placing the film in an aqueous solution of ascorbic acid, and carrying out heating reduction to obtain the flexible self-supporting graphene film with the internal doped with metal nano-particles; titrating an aqueous solution of graphene oxide on a solid substrate, and drying the mixed solution to form a film; placing the film in the aqueous solution of ascorbic acid, and carrying out heating reduction to obtain the flexible self-supporting graphene film with the surface doped with metal nano-particles. The method has the advantages of simplicity in preparation, few reaction steps, convenience in operation, simple requirements of devices and technologies, cheap raw materials, low cost, and realization of the graphene film with the internal doped with metal nano-particles and the graphene film with the surface doped with metal nano-particles.

The invention discloses a new method for performing data correction by using cell metabolite relative content as cell number index. The new method is characterized by comprising the steps of: realizing the fast quenching of biochemical metabolism in cells by using a simple sample pre-processing method; efficiently extracting small molecule metabolites by an optimized extraction solvent; and roundly and half quantitatively measuring the small molecule compounds in a biological sample by a metabonomics method based on measuring technology like mass spectrum, nuclear magnetic resonance and the like so as to identify a quantitative index of symbolic metabolites in cells to be as the cell number, and regarding the quantitative index as a weight factor to perform data correction. Compared with the quantitative index of the prior cell number, the method is simple, fast and with good repeatability; additional experimental working and the error caused by the additional working are reduced; the accuracy and reliability of experiment result are improved; the difficulty for relatively quantifying the number of in vitro cultured cells is solved; a new displacing method is supplied for cell metabonomics and relevant researches; and the method can be widely used in the relevant researches in the field of cell molecular biology.

The invention discloses a dynamic pressure regulating gas absorption and desorption experiment device based on a high-pressure volumetric method. The dynamic pressure regulating gas absorption and desorption experiment device comprises a dynamic pressure regulating inflation system, a vacuum degassing system, a gas absorption and desorption system, a constant-temperature system, a metering systemand a data processing system, wherein the dynamic pressure regulating inflation system is provided with a high-pressure gas cylinder, a pressure regulating valve, a high-pressure valve, a pressure gauge, a dynamic pressure regulating inflation tank and a three-way connector; the vacuum degassing system is provided with a vacuum pump, a motor, a vacuum diaphragm valve, a vacuum gauge tube, a vacuumgauge, a vacuum tank path, a high-pressure valve and a three-way connector; the gas absorption and desorption system is provided with an absorption and desorption tank, a pressure gauge and a high-pressure valve; the constant-temperature system is provided with a super constant-temperature water bath tank; the metering system is provided with a flow meter, a leveling bottle, a measuring cylinder,a glass piston, a sampling bag and a lifter; the data processing system is provided with a pressure sensor, a data collector, a computer and a printer. The dynamic pressure regulating gas absorptionand desorption experiment device disclosed by the invention has the advantages of convenience in operation, high experiment accuracy, accurate results and ability in achieving gas absorption and desorption experiments.

The invention discloses a heat accumulation capsule for decreasing the temperature of main stream smoke of cigarettes. The heat accumulation capsule comprises a wall material and a core material, wherein the core material contains an inorganic phase-change material; and the wall material is polylactic acid; and the inorganic phase-change material is disodium hydrogen phosphate dodecahydrate. The invention further discloses a preparation method of the heat accumulation capsule. The preparation method comprises the steps of simultaneously dropwise adding a core material solution and a wall material solution into concretion liquid respectively through inner holes of an electrostatic spinning coaxial needle head and gaps between inner and outer holes of the electrostatic spinning coaxial needle head, stirring, and carrying out solid-liquid separation, washing, cooling and concretion. The preparation method is different from existing commonly adopted chemical methods, and the inorganic phase-change material is coated by virtue of a physical method. Compared with the chemical methods, the preparation method has the advantages that the complicated chemical reaction steps are reduced, theovermuch chemical solvent is not used, a device is simple, and the particle size of the heat accumulation capsule is controllable.

The invention discloses a method for activating a carbon fiber by colloid copper and preparing a chemical plating copper-nickel duplex metal layer by one-step. According to the method for activating the carbon fiber by the colloid copper and preparing the chemical plating copper-nickel duplex metal layer by one-step, a colloid copper activating method is adopted to replace traditional palladium, cheap material copper sulfate is adopted, an optimized activating solution has good stability and high activation properties, and a composite plate formed during subsequent chemical plating is even andcontinuous; in addition, compared with a two-step method of nickel plating after copper plating, a one-step method of chemically plating the copper-nickel duplex metal layer reduces the using amountof copper sulfate and nickel sulfate, and the one-step method is faster and more efficient; and sodium monophosphate is used for replacing formaldehyde as a reducing agent, and environmental pollutionand harm to human bodies are reduced.

The invention relates to a method for preparing a SiBCN-containing thermally-polymerisable ceramic precursor. The method comprises the steps of enabling vinyl dichlorosilane and hexamethyldisilazane, which are in a certain ratio, to react at a certain temperature, adding a certain amount of NaBH4 reducer into the reaction product for reduction after reaction is complete, then, adding dimethyl vinyl chlorosilane into the reduced reaction product to continue reaction, heating up the reaction product to distill off a solvent and some unreacted raw material molecules, finally, adding a solvent n-hexane into the reaction product to wash the reaction product, thereby obtaining a colorless viscous liquid product, i.e., the soluble and fusible SiBCN ceramic precursor. According to the synthesis method, the operation is simple and convenient, experimental conditions are easily controlled, experimental steps are reduced, the cost is low, the prepared SiBCN ceramic precursor is soluble and fusible, has good processability, is good in fluidity and can be subjected to thermal polymerization directly, the yield of the prepared product can reach 60% the input amount of chlorosilane, and the yield of sintered ceramics can reach 64%.

The invention relates to a simple measurement device for the heat conductivity coefficient of porous ceramics. The device comprises a heater arranged at the bottom, wherein the top of the heater is provided with a heat source, the top of the heat source is provided with an upper sheet copper and a lower sheet copper which correspond to each other up and down, the lower sheet copper is directly contacted with the heat source to be taken as a hot end, and the upper sheet copper is taken as a cold end; when the heat conductivity coefficient is measured, the porous ceramics to be measured is arranged between the upper sheet copper and the lower sheet copper; the upper sheet copper and the lower sheet copper are respectively connected with a thermoelectric couple; and a heat preservation cover is packed on the upper sheet copper, the porous ceramics to be measured and the lower sheet copper, and the top of the outer side of the heat preservation cover is provided with a counter weight block. The simple measurement device is used for computing the heat conductivity coefficient by adopting the Fourier heat transfer law, and is simple in structure; parts used for building the device, i.e. the heater, the heat source, the sheet coppers, the thermoelectric couples and the like, are not expensive, so that the simple measurement device is low in cost; and the experimental the number of operation steps for measuring the heat conductivity coefficient by using the device is small, and compared with the measurement by using a steady-state method which needs a plurality of hours for once, the measurement by using the device commonly needs 10-20min for once.

The invention relates to a fluorescence in-situ hybridization technology, and discloses a fluorescence in-situ hybridization method capable of improving covariance. The fluorescence in-situ hybridization method capable of improving covariance comprises the steps of slide specimen preparation, probe and chromosome covariance and hybridization, washing and dyeing after hybridization, and signal detection. According to the fluorescence in-situ hybridization method capable of improving covariance disclosed by the invention, a method of probe and chromosome covariance and hybridization is adopted on the basis of the prior art, the operation is simple, the reaction steps are reduced, experimental time is shortened, and a toxic and teratogenic denaturant does not need to be used, thus decreasing experimental cost, and avoiding the harm of the denaturant to experimental operating personnel and environment. The invention provides a fluorescence in-situ hybridization method capable of improving covariance, which is accurate in location, high in sensitivity, high in experimental efficiency, good in stability, simple to operate, and has environment-friendly effect.

The invention relates to a low-temperature, halogen-free, low-solid-content modified rosin-type welding flux. The welding flux is prepared form, by mass, 10-15% of modified rosin, 1-5% of organic acidactivator, 0.35-1% of glycerin, 0.009-1% of benzotriazole, 0.3-1 % of triethanolamine, a surfactant and the balance organic solvent, wherein the surfactant is 0.5-3% of the mass of the organic acid activator. The prepared low-temperature, halogen-free and low-solid-content modified rosin-type welding flux prepared has good fluidity, no precipitation and delamination phenomena, no halogen elements, a small amount of modified rosin, a good spreading rate, good weldability, bright and full welding spots and less residues after welding, little corrosion is caused, and cleaning is not needed; therequirements for environmental protection are met; an operation method is sample, the temperature is moderate, there are less experimental steps, the cost is low, less by-products are generated in a synthesis process and are easy to remove, and the synthesis time is short.

The invention discloses a method for eliminating a digital holographic zero-order image through one-step arbitrary angle phase shift. A linkage coaxial dual-reference-light light path is provided for the first time; by adjusting a beam splitting crystal loaded to an inching translation platform in the light path, the phase shift magnitudes of two beams of reference light are guaranteed to be -alpha and 2alpha when the inching translation platform displaces. Based on the light path, a matched algorithm is provided, the existing phase shift zero-order digital holographic elimination technology is changed into one-step phase shift from multiple steps of phase shift, the phase shift magnitude is arbitrary, and the error produced by multi-step phase shift and movement of the inching translation platform is eliminated, so the phase shift technology is easier and more convenient and the operability is higher.

The invention discloses a micro-fluidic chip for extracting total nucleic acid of bacteria in human whole blood. The chip comprises a positioning hole and a main body. The main body comprises a sampling hole formed in a sampling cavity, the sampling cavity, a one-way valve, a nucleic acid extraction material, a nucleic acid extraction cavity, a PCR reaction cavity, a buffer solution I cavity, a buffer solution II cavity, an RNA enzyme inhibitor cavity, an MIX1 cavity, a DNase I cavity, a MIX2 cavity, a 70% ethanol cavity, a buffer solution II cavity, an MIX3 cavity, a PCR Master Mix cavity, aPCR primer cavity, a PCR reaction probe cavity and a buffer solution V cavity. The chip provided by the invention can split the bacteria quickly to release RNA, and is simple and convenient to operate, so that the extraction efficiency is improved greatly; the detection limit is reduced to the lowest, so that the probability of leaked detection is avoided; the operating process of pathogenic bacteria detection is simplified, and the detection error caused by manual operations is reduced.

The invention discloses a vanadium trichloride mixed powder and the application thereof in quickly measuring the nitrate content in the food. The vanadium trichloride mixed powder is composed of sodium chloride, sulfamic acid, sulfanilic acid, naphthyl ethylenediamine hydrochloride and vanadium trichloride. When the vanadium trichloride mixed powder is applied to quickly measure the nitrate content in the food, firstly, the food is homogenized and then metered in volume by using the acetic acid solution. After that, the homogenized food is oscillated and filtered to obtain a sample solution. Secondly, the vanadium trichloride mixed powder is added into the sample solution, and the obtained product is shaken uniformly, subjected to water bath and cooled. The light absorption value of the obtained product is measured and then the nitrate content is calculated according to a standard curve. According to the technical scheme of the invention, the method is simple and easy to operate, fast, short in consumed time, sensitive, accurate, small in reagent consumption, and low in cost. Meanwhile, an operator can master the method without being specially trained. Moreover, reagents adopted in the method are not volatilized, free of odors and extremely small in toxicity to human bodies. The mixed power is good in stability. The detection reagent is harmless to the environment and human bodies. Therefore, the vanadium trichloride mixed powder has a wide application prospect in detecting the nitrate content in the rapid detection field of food especially the rapid detection field of fresh vegetables.

The invention relates to a method for screening lactic acid bacteria capable of bacteriocin from plant source materials. The method comprises the following steps: treating the plant source materials; separating and screening out the lactic acid bacteria; screening a lactic acid bacterium strain capable of producing antibiotic substances by using a method of fermenting a supernatant fluid by use of the strain to carry out an antibiotic test; eliminating interference of small molecules by using dialyzate of the supernatant fluid to carry out the antibiotic test, and screening out the lactic acid bacteria capable of producing the bacteriocin; and performing an antibiotic test by using a protein crude extract which is precipitated by use of ammonium sulfate, and performing a crude extract enzymolysis test by using various proteases to prove that the lactic acid bacterium strain capable of producing the bacterocin can be effectively screened by utilizing the method. In the method, the method for separating and purifying the lactic acid bacteria capable of producing the bacteriocin is improved, the fermented supernatant fluid is dialyzed by a dialysis bag with smaller cut-off molecular weight, so that the inhibition effect of small molecular antibiotic substances in the fermented supernatant fluid on indicator bacteria can be eliminated, and the activity of the bacteriocin can be guaranteed. By utilizing the method, the experimental steps are reduced, and the screening efficiency is improved.

The invention relates to a device for rapidly testing effective thermal conductivity of a porous metal material on the basis of a guarded hot plate method. The device comprises a guarded hot plate testing unit located at a hot end of a to-be-tested sample, a temperature control and timing unit connected with the guarded hot plate testing unit, a water cooling unit located at a cold end of the to-be-tested sample as well as a temperature acquisition unit for acquiring the temperature of the cold end and the hot end of the to-be-tested sample; the guarded hot plate testing unit comprises a main heater, a thermal insulating layer, a side auxiliary heater and a bottom auxiliary heater; the water cooling unit comprises a water cooling head covering the upper end surface of the to-be-tested sample, and the main heater covers the lower end surface of the to-be-tested sample. The invention further relates to a method for rapidly testing the effective thermal conductivity of the porous metal material on the basis of the guarded hot plate method. Through temperature control and time accumulation, the thermal flux density in the guarded hot plate method is measured, and the device and the method have the characteristics that the device cost is lower, the circuit is simple to connect and convenient to build, the testing speed is rapider and the like. The invention belongs to the technical field of testing of heat transfer performance.

The invention relates to a child-friendly implicit attitude test method and device. The child-friendly implicit attitude test method comprises: step S1, a touch display screen randomly displays a target stimulus target at the center, and respectively displays a positive attribution key and a negative attribution key at the lower left corner and the lower right corner; step S2, the reaction time and the correctness that a person to be tested executes a compatible mission are recorded; step S3, the step S2 is executed repeatedly until the compatible mission is executed for M times; step S4, the reaction time and the correctness that the person to be tested execute an incompatible mission; step S5, the step S4 is executed repeatedly until the incompatible mission is executed for N times; step S6, an implicit attitude index is calculated. The child-friendly implicit attitude test method and device provide tool supports for the related fields including development of implicit attitudes and explicit attitudes, and implicit attitudes and infant behaviors, and can make up of the defect that a child implicit attitude measurement tool is lacked at home and abroad; and the attitude test method and device are convenient and practical, have a friendly interface and are simple to operate.

The invention relates to an integrated printing device and printing method of a continuous fiber embedded material, and belongs to the technical field of function material 3D printing. A polymer cylinder filamentation stick with a hole is preformed, and a continuous fiber penetrates through the hole of the filamentation stick; the filamentation stick is tightly clamped in a filamentation barrel; apush rod pushes the filamentation stick downwards; the filamentation stick passes through a heating area; molten polymer flows downwards to wrap the surface of the continuous fiber; finally, a formedwire comes out from the bottom of the filamentation barrel; the formed wire passes through a measuring and shearing mechanism, is shorn when the diameter does not meet the requirement and directly enters a feeding mechanism when the diameter meets the requirement; the feeding mechanism unceasingly feeds the formed wire downwards; and the formed wire is fed into a printing head part, and printingis achieved. The integrated printing device and printing method have the advantages that adhesiveness between the fiber and the polymer is good, local accumulation of the continuous fiber cannot be generated, formed wires of different diameters can be obtained through printing according to requirements, and printing of continuous fiber embedded type high-melting-point polymer is achieved.

The invention discloses a staining method of ciliates, belongs to the technical field of biology, and provides a staining method of ciliates to solve the problems that a traditional ciliate staining method is long in staining time and fussy in staining step, and cytostome and cilium cannot be clearly displayed. A saturated ethyl alcohol mercuric chloride solution is adopted as a fixing agent to carry out pre-fixation on ciliate bodies. In addition, before the pre-fixation operation, to-be-produced ciliate bodies are attached to a to-be-dyed glass slide; a plurality of steps such as dropwise adding protein glycerinum water and collodion are omitted. The staining method disclosed by the invention is convenient to operate; the sensitivity and the staining effect of silver staining of the ciliate protein are greatly improved; and rapid, efficient and accurate display of infraciliature in morphological classification and cytological studies of the ciliates is achieved.

The invention discloses a primer group, a kit and a method for detecting hypertension drug related gene polymorphism. The primer group is selected from SEQ ID NO.1-SEQ ID NO.360 nucleotide sequences.The primer group can directly perform PCR after multiple PCR capturing; the primer group and the kit are simple to operate, and experiment steps and check are reduced; besides, consumable and labor cost for construction of a DNA library can be further reduced; drug kinds guided by gene sites corresponding to 180 pairs of primers comprise fix common hypertension treatment drugs and hypertension complication drugs including anti-freezing, anti-platelet, sugar-reducing, aldosterone and statin common drugs, selection of group treatment schemes for complications such as newly diagnosed hypertension, H type hypertension, refractory hypertension and hypertension atherosclerosis can be guided, so that patients can be effectively treated as early as possible.