Micro-fluidic chip based on magnetic fluorescence composite particle

A technology of microfluidic chips and composite particles, which can be used in fluorescence/phosphorescence, laboratory containers, and material analysis by optical means. Ease of application, rapid on-site detection, and effect of eliminating interference

Active Publication Date: 2017-08-01
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention analyzes sample trace substances through magnetic enrichment separation reaction and fluorescent optical detection technology, which meets the requirements of rapid, sensitive and convenient detection of low-concentration substances in trace samples, and solves the problem of complex and expensive instruments and equipment in the existing detection methods, which are not convenient enough , slow detection speed and other issues, it has good application value in the field of POCT detection

Method used

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  • Micro-fluidic chip based on magnetic fluorescence composite particle
  • Micro-fluidic chip based on magnetic fluorescence composite particle
  • Micro-fluidic chip based on magnetic fluorescence composite particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Preparation and detection of mAlb (urinary microalbumin, urinary microalbumin) by a planar optical microfluidic chip based on magnetic fluorescent composite nanoparticles.

[0055] See figure 1 , 2 , the disposable planar optical microfluidic chip of the present invention is mainly composed of a chip bottom plate 1 , a chip channel layer 3 and a chip top cover plate 2 and the like. Wherein the bottom plate of the chip 1 is designed with a transparent material and has an inward groove at a specific angle in the incident area; The liquid channel 3-4 and the waste liquid storage area 3-5; the chip upper layer cover plate 2 has a hydrophilic film layer. The capture antibody / capture recognition reagent 7 is immobilized on the lower side of the optical detection area 3-3, and the labeled antibody / labeled recognition reagent 8-magnetic fluorescent composite particle 9 is pre-immobilized on the sample injection area 3-1 and the sample injection channel area 3-2. T...

Embodiment 2

[0061] Example 2: see figure 1 , figure 2 , the disposable planar optical microfluidic chip of the present invention is mainly composed of a chip bottom plate 1, a chip channel layer 3 and a chip upper cover plate 2 and the like. Wherein the bottom plate of the chip 1 is designed with a transparent material and has an inward groove at a specific angle in the incident area; The liquid channel 3-4 and the waste liquid storage area 3-5; the chip upper layer cover plate 2 has a hydrophilic film layer. The capture antibody / capture recognition reagent 7 is immobilized on the lower side of the optical detection zone 3-3, and the labeled antibody / labeled recognition reagent 8-magnetic fluorescent composite particle 9 is pre-immobilized on the upper side of the optical detection zone 3-3. The detection device 10 is placed under the optical detection area 3-3, and the electromagnetic module 11 is on the upper and lower sides of the optical detection area 3-3.

[0062] Wherein, the c...

Embodiment 3

[0067] Embodiment 3: see Figure 1-3 , the disposable planar optical microfluidic chip of the present invention is mainly composed of a chip bottom plate 1, a chip channel layer 3 and a chip upper cover plate 2 and the like. This embodiment can use image 3 The side-incidence mode of the planar structure shown has a larger total reflection angle, slightly worse excitation efficiency, and better anti-interference effect. Wherein the bottom plate of the chip 1 adopts a transparent material and has a lateral groove design with a specific angle in the incident area; the chip channel layer 3 includes a sample feeding area 3-1, a sample feeding channel 3-2, an optical detection area 3-3, The waste liquid channel 3-4 and the waste liquid storage area 3-5; the chip top cover plate 2 has a hydrophilic film layer. The capture antibody / capture recognition reagent 7 is immobilized on the lower side of the optical detection zone 3-3, and the labeled antibody / labeled recognition reagent 8...

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Abstract

The invention provides a micro-fluidic chip based on a magnetic fluorescence composite particle. The micro-fluidic chip comprises a chip bottom-layer plate, a chip channel layer and a chip upper-layer cover plate which are sequentially arranged from bottom to top, wherein the chip channel layer comprises a sample introduction area, a sample introduction channel, an optical detection area, a waste liquid channel and a waste liquid storage area which are sequentially connected; a capture recognition reagent is fixed to the lower side of the optical detection area; mark recognition reagent-magnetic fluorescence composite particles are pre-fixed on the inner upper sides of the sample introduction area, the sample introduction channel or the optical detection area. The invention also provides an optical detection device. The micro-fluidic chip provided by the invention can be used for sample trace substance analysis through magnetism enrichment and separation reaction and a fluorescence optical detection technology, meets the requirements on quick, sensitive and convenient detection of low-concentration substances in a micro-sample, solves the problems of complicated and expensive instrument equipment, inconvenience, slow detection speed and the like of an existing method, and has a favorable application value in the POCT (Point-of-Care Testing) detection field.

Description

technical field [0001] The invention relates to the technical field of biosensor detection, in particular to an optical microfluidic chip using microfluidic technology, total reflection or evanescent field optical technology and magnetic fluorescent particle technology. Background technique [0002] Total reflection fluorescence technology is a new type of optical detection technology, which uses the evanescent field generated by total reflection to excite fluorescence, so that the fluorescent particles within 200 nanometers of the incident light interface are excited to produce fluorescence, while the incident light and redundant other areas Fluorescent particles will not interfere with the detection, and the color and turbidity of the sample will not affect the detection. Therefore, the detection signal-to-noise ratio is high. The currently developed total reflection fluorescence microscopy technology is not conducive to on-site detection due to the need for complex micros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/64G01N33/543
CPCB01L3/502753B01L2200/10B01L2300/0887B01L2300/168G01N21/6428G01N33/54326G01N33/54346
Inventor 刘春秀蔡浩原高然李彤
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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