A method for the detection of arsenic ions based on fluorescence resonance energy transfer of aptamer chain-black phosphorus nanosheets

A technology of energy resonance transfer and nanosheets, which is applied in the field of arsenic ion detection, can solve the problems of complex arsenic ion detection steps, high cost, time-consuming and laborious, and achieve short detection time, good selectivity, and improved detection sensitivity and detection range. Effect
CN109239040BActive Publication Date: 2020-12-15TAIYUAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TAIYUAN UNIV OF TECH
Publication Date
2020-12-15

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Abstract

The invention relates to an arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer, belonging to the technical field of arsenic ion detection. The method solves problems that existing arsenic ion detection method has complex steps, takes time and effort and is high-cost. According to the detection method, As<3+> in a to-be-detected solution is detected quantitatively according to change of fluorescence strength recovery level of a fluorescent dye ROX in a reaction system by using a fluorescence quenching characteristic of black phosphorus nanosheet and an As<3+> probe comprising black phosphorus nanosheet and As<3+> aptamer chain marked with the fluorescent dye ROX. The detection method has the advantages of fast detection speed, simple detection steps, high detection sensitivity, and high selectivity.
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Description

technical field

[0001] The invention belongs to the technical field of arsenic ion detection, and in particular relates to an arsenic ion detection method based on fluorescence energy resonance transfer of aptamer chain-black phosphorus nanosheets. Background technique

[0002] In recent years, with the development of industrialization, heavy metal pollution has become more and more serious, especially arsenic pollution has aroused people's great concern. Arsenic is a highly toxic carcinogen, among which trivalent arsenic compounds are more toxic than other arsenic compounds, and the toxicity of trivalent arsenic is about 60 times that of pentavalent arsenic. Arsenic pollution is widely distributed, and it is reported that more than 20 countries have suffered from arsenic pollution, which has caused many acute and chronic health problems, such as skin damage, circulatory system diseases and other high-risk diseases. To make matters worse, arsenic cannot be removed from the ...

Claims

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