3results about How to "The detection method is simple and fast" patented technology

The application discloses CircSESN1 a gene, an overexpression vector thereof, a specific primer pair, a detection kit and application thereof. CircSESN1 The application also discloses application of the gene, the overexpression vector, the specific primer pair or the fluorescent quantitative detection kit in regulation of chicken skeletal muscle growth and development. CircSESN1 The application further discloses a method for inhibiting proliferation and / or promoting differentiation of chicken myoblasts. The detection kit provided by the application can be used for detecting

The application discloses application of a GSDME gene SNP molecular marker in judgment of heat adaptability of dairy cows and belongs to the technical field of animal molecular breeding. The GSDME gene SNP molecular marker is chr4:71097215A>G, a bovine reference genome ARS-UCD2.0; the SNP molecular marker gene polymorphism is A or G, and can be used for judgment of heat adaptability of dairy cows. The chr4:71097215A>G is a missense mutation subjected to positive selection, and the mutation causes a missense mutation (p.Tyr43Cys) of a pore-forming domain at the N terminal of a GSDME protein. The heat tolerance of a short-hair Holstein cow individual with the genotype GG of the molecular marker is significantly higher than that of individuals with the genotypes AA and AG. The marker is derived from a Senepol cow introgression region and subjected to positive selection, the detection method is simple and fast, is suitable for screening of heat-tolerant individuals and gene editing breeding practices, and has a clear biological background and breeding value.

The application discloses a method for improving resistance to wheat scab QFhb-2DL and QFhb- 3BL The application discloses a method for improving resistance to wheat scab, a molecular marker, a primer, the molecular marker comprising a dCAPS marker of QFhb-2DL and / or a KASP marker of QFhb-3BL . The application first develops a molecular marker closely linked to QFhb-2DL and a molecular marker closely linked to QFhb-3BL . Under the premise of stabilizing the resistance to stripe rust, dwarf, large spike and multiple spike characteristics of a wheat variety in the upper reaches of the Yangtze River, the application realizes molecular design breeding by using the developed new marker closely linked to the locus, and quickly introduces the disease-resistant genotypes of QFhb-2DL and QFhb-3BL into the wheat variety in the upper reaches of the Yangtze River, breeds a strain which is resistant to scab and has a yield significantly higher than that of a control, and realizes a breakthrough in the resistance to scab in the upper reaches of the Yangtze River.