150results about How to "The detection method is simple and fast" patented technology

The present invention discloses a primer, a method and a kit for detecting the animal clonorchiasis sinensis specificity, a nucleotide sequence of an upstream primer of the primer is represented by SEQ ID NO:1, a nucleotide sequence of a downstream primer is represented by SEQ ID NO:2. the present invention implements an PCR amplification to a detectingformwork DNA by the primer, an amplifying outcome yield is processed by an agarose gel electrophoresis and observed under an ultraviolet light, if it is a positive result, there will be a specificity amplifying band, otherwise there will not be a band. According to an ITS zone sequence database OF THE animal clonorchiasis sinensis, the invention designs the primer, builds a rapid, special and sensitive PCR method, and it is capable of authenticating the animal clonorchiasis sinensis accurately. The operation of the kit of the invention is simple and programmable, the method specificity is strong, the sensibility is high, the result judgement is objective, and the invention is capable of being used for diagnosing the animal clonorchiasis sinensis and inquiring epidemiology.

The invention provides a preparation method of urea detection test paper in dairy and a detection method of urea; urease source powder is taken and mixed with pH indicator with the pKHIn more than 6.8; the mixture is diluted by deioned water and the filter solution or the upper suspension liquid after natural deposition is taken, uniformly fixed on a carrier with good water absorption by the forms of coating, printing or soaking, and dried subsequently, thus forming the urea detection test paper; the detection method of the dairy comprises the steps as follows: firstly, common pH test paper is used for detecting the dairy to be detected; whether the dairy to be detected is in normal pH range 6.3-6.8 or not is checked; if so, the urea detection is directly carried out; if not, the pH value is adjusted to the normal pH range and the urea detection is carried out subsequently; one piece of urea detection test paper dips the dairy to be detected and the color change of the test paper is observed; if the color of the test paper is not changed, the dairy is qualified; if the color of the test paper changes, the dairy is mixed with the urea, thus carrying out qualitative judgment on the dairy; and the displayed color is compared with a standard colorimetric card, so as to carry out semi-quantitative detection of the urea in the dairy.

The invention relates to an on-site wind pressure resistance equivalent static-load detection method and device. The method comprises the following steps of: (1) opening and closing an openable part on a tested curtain wall component for at least five times and finally closing; (2) fixedly arranging a fixed frame at one side of the tested curtain wall component and fixedly connecting a displacement meter matched with the tested curtain wall component on the fixed frame; (3) fixedly arranging a counter-force supporting frame at the other side of the tested curtain wall component; (4) arranging an airbag connected with a manometer and an air pump in a gap between the counter-force supporting frame and the tested curtain wall component; (5) preliminary pressing; and (6) detecting deformation. By using the detection method, the wind pressure resistance of an existing curtain wall can be rapidly and accurately detected to provide guarantee for the normal use of the curtain wall, the detection method is simple, rapid, suitable for popularization and use in a large area, and the detection device is extremely simple in structure, low in manufacture cost and convenient to operate.

The invention relates to a method and a device used for rapidly detecting paper and fluorescent whitening agent in a paperboard. The method includes the following steps. Firstly, ultraviolet light, the wave length of which is bigger or equal to 244nm but smaller or equal to 375 nm, is used to irradiate directly on a matter to be detected. Then the next step is to observe that whether the mater to be detected is provided with purple or blue white fluorescence or fluorescence reflection points. If the matter to be detected is equipped with purple or blue white fluorescence or fluorescence reflection points, the matter contains fluorescent whitening agent. If not, the matter to be detected contains no fluorescent whitening agent. The device includes a base. And a stent extends from the base. The stent is equipped with a light source, which can emit the ultraviolet with the wave length. The detection test result proves that the fluorescence detection device has the advantages of low cost, high detection sensitivity, visual and practical performances, accurate and reliable result and convenient and rapid use.

The invention discloses a main switch fault detection method for an asymmetric half-bridge-type power converter of a switched reluctance motor. The method comprises the following steps: detecting the instantaneous value of each phase current of the switched reluctance motor power converter, calculating the difference between the two of the normalized current averages, and treating each difference as an element to construct the fault feature quantity set Ek; determining the condition which the element in the set belongs to in the fault diagnosis table by comparing the relationship between the elements in the set and the diagnostic threshold; detecting the fault occurrence, the fault type and the fault phase according to condition details in the table; if the fault type is open, identifying the fault element by calculating the demagnetization time of the fault phase current; and if the fault type is short circuit, identifying the faulty element by judging the main switch on and off state. The method can precisely and rapidly detect the fault details, is suitable for a variety of phases of switched reluctance motor power converters without adding any additional sensors, and is high in versatility, high in reliability and good in implementation effect.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention discloses an immunodetection kit specially used for rice black-streaked dwarf virus in single planthopper and a using method thereof. Specific polyclonal antibodies of the rice black-streaked dwarf virus (RBSDV) are utilized to build a dot immuno-binding assay (DIBA) detecting method which is used for building the detection kit for rice black-streaked dwarf virus, main reagents of the kit are working solution or concentrated solution, and the kit has the characteristics of convenient use, simple operation, and the like. And simultaneously, the use of the rice black-streaked dwarf virus detection kit can be a standard program or a reduced program, which can greatly broaden the using ranges and applicable persons of the kit and facilitate the population and application of the technique to establishment units, and further, the kit is applied in the fields of plant disease prognosis and prediction and the formulation of prevention and curing strategies, and the like. In addition, the kit can be used for measuring the inoculation strength in varietal resistance seed selection of rice black-streaked dwarf virus, and the like.

The invention relates to a detection method for content of heavy metal in preserved vegetable and in particular relates to a method for detecting the content of heavy metals, such as lead, arsenic, mercury, cadmium, aluminum, copper and chromium in preserved vegetable by use of ICP-MS (Inductively Coupled Plasma-Mass Spectrometry). The method comprises the steps of selecting instruments and reagents, preparing a standard solution, digesting a sample, detecting by use of the instruments and the like. The method is capable of quickly and accurately detecting the contents of lead, arsenic, mercury, cadmium, aluminum, copper and chromium in the preserved vegetable.

The invention discloses a granary grain storage quantity detection method based on a three-dimensional force sensor. through arranging the three-dimensional force sensor on the side wall of the granary, the static friction force between the granary wall and the real-time grain quantity can be measured accurately and directly, the grain bulk bottom pressure is acquired through a pressure sensor atthe bottom part of the granary, and through a detection model W=PA+PB (PA is the granary bottom pressure and the PB is the static friction force generated on the grain side surface),, the granary grain storage quantity can be quickly and effectively calculated. The method of the invention has the advantages that simplicity and quickness are realized; the operation is easy; the accuracy is high; through measuring the static friction force between the granary wall and the real-time grain quantity, the granary grain storage quantity can be simulated and calculated; the problem of analyzing and estimating the granary side pressure during the grain detection process is skipped; the granary grain storage quantity detection errors are greatly reduced; theoretical significance is realized for studying the complex pressure distribution inside the granary; and a more direct and effective technical means is provided for grain quantity detection.

The invention discloses a method for simultaneously measuring residue amounts of various neonicotine type drugs in royal jelly and metabolites thereof. The method comprises the following steps of: (1)removing protein in a to-be-measured sample to obtain extract; (2) carrying out purification on the extract by adopting an absorbent to obtain purified solution, and after drying the purified solution, making up to volume with a loading solvent to obtain to-be-measured solution, wherein the absorbent is formed by mixing anhydrous magnesium sulfate, N-primary secondary amine and C18 powder in a mass ratio of 10:6:3; (3) drawing a standard curve; and (4) carrying out sample injection on the to-be-measured solution under a preset liquid chromatography tandem mass spectrometry condition, and according to the standard curve and a detection result of the to-be-measured solution, calculating content of the corresponding neonicotine type drugs or metabolites in the to-be-measured solution. According to the invention, a dispersive solid-phase extraction purification technology is adopted to carry out purification on the extract; the method is simple and convenient in step, easy to operate, green and friendly; purification only requires 5 to 10 minutes; extraction efficiency is high; cost of consumables is only 5 to 7 yuan each time; and cost is low.

The invention relates to a method for detecting a microorganism based on a CRISPR-Cas13a system. Firstly, a bacterium to be detected is pretreated, a genome of the bacterium to be detected is extracted, RNA, called target RNA, is obtained through two steps of PCR amplification and in-vitro transcription. When the target RNA exists, the attached cleavage ability of a Cas13a-crRNA compound can be motivated, namely single-stranded RNA is cleaved. A fluorophore is modified at one end of the RNA, and a quencher is modified at the other end of the RNA. When the Cas13a-crRNA compound detects the corresponding Target RNA, a fluorescent probe can be cleaved, the increase of a fluorescence value is caused, the increase of the fluorescence value is in a linear correspondence relationship with the bacterium to be detected, and the microorganism is detected. The method has the characteristics of excellent reliability, extremely high sensitivity, high specificity, easiness in implementation and short detection time, and has great potential for detection of the microorganism.

The invention relates to a fingerprint detection method for Shenshuaining granule. The detection method employs HPLC for detection, and comprises the following steps: a, determining chromatographic condition; b, preparing a tested object solution; and c, establishing fingerprint. The chromatographic conditions in the step comprise that the chromatographic column is C18 chromatographic column; the mobile phase comprises a mobile phase A and a mobile phase B with the ratio of 5%:95%-100%:0%, the mobile A is acetonitrile or methanol, and the mobile phase B is water, a phosphoric acid aqueous solution with the concentration of 0.01%-0.5% or a formic acid aqueous solution with the concentration of 0.01%-0.5%; the detection wave length is 190-450 nm; the column temperature is 20-40 DEG C; the volume flow is 0.8-1.2 mL / min; the sample size is 5-100 mu L; and the elution program employs gradient elution and the elution time is 80-100 min. The detection method is simple, rapid, easy to operate, high in precision, good in stability and good in reappearance, possesses many characteristic peaks, and can well provide quality control basis for production of Shenshuaining granule.

The invention discloses a DNA methylation detection method. The DNA methylation detection method comprises the following steps: 1) selecting a DNA sequence; 2) hybridizing DNA double strands. The invention further discloses a DNA methylation detection kit. Through direct hybridization of the DNA strands and by virtue of interaction between the graphene oxide and the DNA, cytosine can be methylated in CpG island region through various methylation processes; through specific position recognizing and CpG position cutting functions of the restriction enzyme, an aim of detecting the methylated DNA is achieved; compared with the existing detection technology, the DNA methylation detection method has the characteristics of simpleness, fastness and convenience in operation; by the DNA methylation detection method, the effect of chemicals harmful to a human body on the DNA, namely DNA methylation, can be detected.

The invention provides a fault arc detection method and device, a computer device and a computer readable storage medium. The method comprises the steps of acquiring a detection current signal; sampling the detection current signal at a preset sampling frequency to obtain current sampling signals; carrying out flat shoulder judgment according to the current sampling signals of a plurality of half-wave periods in the detection current signal, and performing statistics on the number of flat shoulders with the half-wave periods; and when the number of the flat shoulders exceeds a first preset number in a preset duration, sending out a fault arc protection signal. The device provides an execution module for the method. The computer device is provided with a processor; when the processor executes a program, the fault arc detection method can be realized. The computer program is stored in the computer readable storage medium and used for realizing the fault arc detection method. The occurrence of pure resistive load fault arc can be rapidly and effectively judged, and a circuit can be effectively protected.

The present invention discloses a method for simultaneous detection of a plurality of content of non-protein nitrogen-containing compounds in milk by liquid chromatography-tandem mass spectrometry, the method is as follows: adding a protein precipitant into a to-be-tested sample, whirling, centrifuging, adding a degreasing agent into supernatant, whirling, centrifuging, removing a degreasing agent layer, concentrating a lower clearing liquid, dissolving with ammonia acetonitrile, whirling, performing column chromatography with a HLB solid phase extraction column, collecting an eluant, concentrating, dissolving with a liquid mixture of a 0.15% formic acid aqueous solution (containing 5mM of ammonium acetate) and acetonitrile in the volume ratio of 1: 9, and filtering to obtain a to-be-tested solution; preparing a standard mixing working solution series; respectively injecting the standard mixing working solution series and the to-be-tested solution under liquid chromatography-tandem mass spectrometry conditions, making a standard curve, and calculating the content of the non-protein nitrogen-containing compounds in the to-be-tested solution according to the standard curve. The method can be used to simultaneously detect the content of at least two of dicyclanil, dicyandiamide, biuret, cyromazine and melamine, and is high in accuracy and strong in sensitivity.

The invention relates to an arsenic ion detection method based on aptamer chain-black phosphorus nanosheet fluorescence energy resonance transfer, belonging to the technical field of arsenic ion detection. The method solves problems that existing arsenic ion detection method has complex steps, takes time and effort and is high-cost. According to the detection method, As<3+> in a to-be-detected solution is detected quantitatively according to change of fluorescence strength recovery level of a fluorescent dye ROX in a reaction system by using a fluorescence quenching characteristic of black phosphorus nanosheet and an As<3+> probe comprising black phosphorus nanosheet and As<3+> aptamer chain marked with the fluorescent dye ROX. The detection method has the advantages of fast detection speed, simple detection steps, high detection sensitivity, and high selectivity.

Photosystem II inhibitor herbicides belong to a main category in chemical herbicides, and psbA genes are target acting sites. The invention provides a specificity PCR (Polymerase Chain Reaction) primer pair (Seq ID No.1 and Seq ID No.2) for detecting the photosystem II inhibitor herbicide resistant digitaria sanguinalis and a PCR detection kit comprising the primer pair. The primer pair provided by the invention has the advantages of high specificity and high sensitivity; PCR experiments prove that (Figure 1) the psbA gene sequence of the digitaria sanguinalis can be amplified; and the amplified sequence contains reported all sites relevant to the resistance. The method has the advantages that the sensitivity and the specificity can realize the fast identification on the field suspected photosystem II inhibitor herbicide resistant digitaria sanguinalis, so that the guidance on the scientific management of resistance weeds in production practice can be realized.

The invention belongs to the technical field of blood detection and particularly relates to a detection method and a detection system of multi-vitamins in a dry blood spot. The invention provides thedetection method of the multi-vitamins in the dry blood spot, which comprises the following steps: S101, adding an internal standard substance in the dry blood spot to obtain a treated dry blood spot;S102, extracting the treated dry blood spot with first extracting liquid to obtain first detection liquid or extracting the treated dry blood spot with second extracting liquid to obtain second detection liquid; and S103, detecting the first detection liquid or the second detection liquid by adopting a liquid chromatography-mass spectrometer, wherein the first extracting liquid is a methanol aqueous solution with a volume percentage of 5-95 percent; and the second extracting liquid is 10-90 percent of methanol aqueous solution. The detection method provided by the invention can detect the multi-vitamins in micro blood within a short time and solves the technical defects of time waste and labor waste in an existing method for detecting the multi-vitamins.

The invention provides a novel coronavirus nucleic acid testing primer pair with anti-mutation performance, a kit and application of the primer pair, and belongs to the technical field of bioengineering and molecular testing. Through preferred design, the primer pair provided by the invention has the excellent anti-mutation performance, and thereby, the situations that testing sensitivity is reduced and even the false negative phenomenon occurs due to gene mutation of a novel coronavirus can be effectively avoided, and rapid high-sensitivity and high-accuracy real-time fluorescence quantification polymerase chain reaction (PCR) testing of the novel coronavirus SARS-CoV-2 is realized; and the primer pair is convenient for a primary level to operate and apply, and accordingly, the primer pair has significant application value in novel coronavirus SARS-CoV-2 epidemic diagnosis and epidemiological investigation.

The invention discloses a large distortion checkerboard image angle point detection method. The method comprises steps that a center point of a communication area of a large distortion checkerboard image is taken as an origin to establish a rectangular coordinate system; contour points consistent with preset conditions are selected according to coordinate values of contour points of the communication area, and the selected contour points are defined as angle point candidate points; taking the center point as the origin, the rectangular coordinate system is turned according to a preset turningmode, and new angle point candidate points are re-defined; after the turning frequency of the rectangular coordinate system reaches the preset turning frequency, the frequency of each contour point defined as the angle point candidate point is acquired, and the angle point candidate points in preset quantity are taken as angle points of the communication area. The invention further discloses a large distortion checkerboard image angle point detection device. The method is advantaged in that the method is simple, convenient and rapid, and the angle points of the single communication area of thelarge distortion checkerboard image can be detected.

The invention discloses a marker for prognosis prediction of liver cancer based on CD11b and CD169 protein molecules. The marker is MRS, and the MRS=0.161*CD11bT-0. 106*CD169T+35. According to the MRSprovided by the invention, a marker of myeloid cells instead of a marker of lymphocytes is utilized; the prediction ability of postoperative recurrence and survival of a patient undergoes multi-center and large-sample verification, and the reliability is higher; the marker can further be used for predicting the curative effect of subsequent sorafenib or TACE treatment on postoperative recurrent patients; prognostic indexes with high prognosis specificity, high sensitivity and high clinical application value can be obtained only by detecting the CD11b+cell density and the CD169+cell density inthe liver cancer tissue, and the detection method is simple and convenient.

The invention discloses a method for detecting n-propyl bromide in leather and textiles. The method is characterized in that the gas chromatography-mass spectrometry combination is used for detection. The detection method is simple, convenient and fast and has high sensitivity. A sample to be detected is in the linear relationship in the range of 0.1-50mg / L, the correlation coefficient is 0.9995, the method detection limit is 0.1mg / L, the standard recovery rate is 96%-112%, and the relative standard deviation (RSD) (n is equal to 7) is less than 2%. The method can be used for detecting the n-propyl bromide in the leather and textiles very well.

The invention provides a hydraulic oil cylinder through-hole identification adjusting device. The hydraulic oil cylinder through-hole identification adjusting device comprises a fixed frame and a through-hole identification mechanism. The through-hole identification mechanism comprises a cylinder body through-hole identification assembly and a piston rod through-hole identification assembly, wherein the cylinder body through-hole identification assembly and the piston rod through-hole identification assembly both comprise rotary driving pieces, clamping pieces and correlation type sensors, therotary driving pieces are installed on the fixed frame, the clamping pieces comprise clamping driving pieces connected with the rotary driving pieces and two clamping hands connected with the clamping driving pieces, the two clamping hands are provided with light inlet channels, and the two clamping hands of the piston rod through-hole identification assembly are provided with positioning grooves; the transmitting terminals and receiving terminals of the correlation type sensors are correspondingly installed on the two clamping hands and correspondingly located at the two opposite ends of thelight inlet channels; and the piston rod through-hole identification assembly further comprises a clamping sensor used for detecting the clamping state of the clamping hands. The through-hole position of a hydraulic oil cylinder can be identified, and the attitude of the hydraulic oil cylinder can be adjusted, so that the requirement of assembly is met. The invention further provides a hydraulicoil cylinder through-hole identification adjusting method and an automatic assembly system.

The invention provides a preparation method for ethyl urethane detection test paper. The preparation method comprises three steps as follows: material preparation: purchasing acetylcholinesterase with activity no less than 220u / g, ethyl urethane with purity of 99%, medium-speed qualitative filter paper with good hygroscopicity, and 2-6-dichlorophenolindophenol acetate; preparing esterase solution; and preparing immobilization of the test paper. The detection method by using the test paper prepared by the method comprises four steps as follows: preparing standard detection solution; preparing a standard colourimetric card; confirming the effectiveness of the test paper; and framing the colorimetry and content. The test paper has the advantages of being fast and convenient, and capable of judging only through visual observation when being used for detecting and removing ethyl urethane harmful substances in alcohol drinks, fermented food and the like, thus not only being applicable in food detection departments, as well as in food production enterprises, sales departments, and users capable of conducting self-detection, and application and popularization range is wide.

The invention provides an intelligent grouting bush and a saturation and damage position detection device and method thereof. The intelligent grouting bush comprises a bush body, sealing rings arranged at openings in the two ends of the bush body and at least three conducting sheets arranged in the inner cavity of the bush body, wherein two conducting sheets are located at the two ends of the inner cavity of the bush body respectively, and the other conducting sheets are arranged at intervals in the axis direction of the bush body. According to the invention, the conducting sheets are connected in series with the power supply and the universal meter to form the detection device, so that the resistance of the grouting material can be detected by using the formed conductive loop after the conductive grouting material is injected, and the saturation and unsaturated positions of the grouting material can be detected according to the resistance values of different positions; in the long-term service process of the anchoring body in the grouting bush, the resistance of the anchoring body at different positions is detected, and the damage position of the anchoring body can be effectively judged; and the detection device provided by the invention is simple in structure, the detection method is simple, convenient and feasible, and the application value is relatively high.

The invention discloses a type D influenza virus fluorescent quantitative PCR primer pair and a kit. The primer pair is designed by intercepting a middle conserved sequence of a type D influenza PB1 sequence published by JQ922306, KM392476, KM392483 and KX768825 on GenBank. The primer pair can be used for preparing the type D influenza virus fluorescent quantitative PCR detection kit, so that virus content of type D influenza virus (IDV) can be detected. The kit comprises the primer pair as prescribed in the claim 1, a positive standard plasma, a TB GreenTM Premix Ex Taq II real-time fluorescent quantitative PCR reagent and ddH2O. The type D influenza virus (IDV) real-time fluorescent quantitative PCR detection kit, which is prepared by the primer pair provided by the invention, is convenient and rapid in detection method, good in repeatability, high in sensitivity and strong in specificity; high-flux samples can be detected; and rapid and high-sensitivity real-time fluorescent quantitative PCR detection can be implemented on the type D influenza viruses.

The invention relates to an electrode subassembly alignment detection apparatus and a detection method thereof. The apparatus comprises an electrode assembly placement unit, a sliding device and an infrared detection device, wherein the electrode assembly placement unit is used for placing and fixing an electrode assembly, the sliding unit comprises a sliding mechanism connected with the electrode assembly and a driving unit used for driving the sliding mechanism to move, the infrared detection device comprises an infrared emission unit used for emitting infrared signals and an infrared detection unit used for detecting whether the electrode assembly is tidily arranged through the infrared emission unit, and the infrared signals can penetrate the electrode assembly placement unit. According to the invention, whether the electrode assembly is tidily arranged can be determined through detecting the infrared signals emitted by the infrared emission unit, and if the infrared detection device cannot detect complete infrared signals, an alarm is automatically given. The detection method is simple and fast, and can effectively improve the detection efficiency of the electrode assembly.

The invention discloses a method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography. The method is characterized by comprising the following steps: (1) preparing floral leaf extraction solution: carrying out ultrasonic extraction on floral leaf powder by using a first extraction solvent, carrying out centrifugal separation, andfiltering to obtain floral leaf extracting solution; (2) preparing an extract sample: carrying out ultrasonic extraction on the floral leaf extracting solution by utilizing a second extracting solvent, and filtering to obtain an extract detection sample; (3) preparing a standard working solution: diluting a cannabinoid standard substance into the standard working solution by using methanol; and (4) high performance liquid chromatography determining: carrying out sample introduction detection on the extract detection sample and the standard working solution by using high performance liquid chromatography to obtain a standard curve, and calculating the cannabinoid component content of the extract detection sample according to the standard curve by using high performance liquid chromatography data processing software. The method has the advantages of short peak appearance time, short detection time, high detection efficiency and accurate and stable detection result, and is suitable for industrial detection.

The invention belongs to the field of analytical chemistry and provides use of a bovine serum albumin-gold-silver alloy nanocluster for detecting alkaline phosphatase, comprising the following steps:1) preparing a BSA-Au / Ag NCs aqueous solution, adding a KMnO4 solution to quench fluorescence of the BSA-Au / Ag NCs aqueous solution to obtain a mixed solution A, and detecting fluorescence intensity to be I1; 2) using alkaline phosphatase to catalyze ascorbic acid 2-phosphate to generate ascorbic acid; 3) mixing the mixed solution A and ascorbic acid to react and detecting the fluorescence intensity I; 4) establishing a standard curve of (I-I1) / I and alkaline phosphatase concentration; and 5) adding the ascorbic acid 2-phosphate to a to-be-tested sample, catalyzing, then, mixing with the mixedsolution A, after the completion of the reaction, detecting the fluorescence intensity I', and comparing the value of (I'-I1) / I' with the standard curve to obtain the alkaline phosphatase concentration in the sample. The method of the invention has the advantages of high sensitivity, good selectivity and simple operation. The alkaline phosphatase level in environmental water is related to the degree of eutrophication of water. The use in the invention has important significance in inventing an alkaline phosphatase fluorescence sensor.

The invention relates to a real-time fluorescent quantitative PCR kit for detecting TRECs and KRECs genes. The kit comprises: 1) a DNA extraction reagent; 2) a standard product containing a TRECs geneinsertion sequence, a KRECs gene insertion sequence and a TRAC gene insertion sequence; 3) a fluorescent PCR reaction solution I containing fluorescent PCR primers and detection probes of the TRECs gene and the TRAC gene; and 4) a fluorescent PCR reaction solution II containing fluorescent PCR primers and detection probes of the KRECs gene and the TRAC gene. The kit has the advantages of high sensitivity, good specificity, simple and rapid detection method, reliable experimental result, realization of screening of immunodeficiency diseases mainly including SCID and antibody deficiency, provision of clinically relevant indications for other primitive immunodeficiency diseases related to T cell and B cell development or other systemic diseases, and facilitation of early diagnosis and treatment.