9results about How to "The detection method is simple and fast" patented technology

The invention discloses a differential transformer type displacement sensor performance detection system and method, and belongs to the technical field of displacement sensor detection. The system is based on a detected displacement sensor and a detected iron core, and comprises a differential transformer type displacement sensor performance detection device, a data collector and a computer, the differential transformer type displacement sensor performance detection device comprises a micrometer micrometer head and a direct current motor device. A tested displacement sensor and a tested iron core are installed on the differential transformer type displacement sensor performance detection device, the tested displacement sensor and a data transmission line of a micrometer micrometer head are respectively connected with a data collector, the data collector is connected with a computer, and a tested displacement range and a collection point are input into the computer. The displacement range is converted into a turn number parameter provided by the direct current motor device, data of the micrometer head and the measured displacement sensor are automatically acquired according to set acquisition points, and a curve is drawn to judge the quality of the measured displacement sensor.

The present invention discloses TAGLN The invention discloses a method for detecting genes affecting the growth and development of skeletal muscle in the chicken embryo stage. TAGLN The invention also discloses a detection kit and a detection method thereof. The detection kit provided by the invention can be used to detect TAGLN The gene expression in chicken muscle tissue and myoblasts is detected in a simple and quick manner. This gene can be used as an important gene for studying the regulatory theory of chicken skeletal muscle growth and development during the embryonic period.

The application discloses CircSESN1 a gene, an overexpression vector thereof, a specific primer pair, a detection kit and application thereof. CircSESN1 The application also discloses application of the gene, the overexpression vector, the specific primer pair or the fluorescent quantitative detection kit in regulation of chicken skeletal muscle growth and development. CircSESN1 The application further discloses a method for inhibiting proliferation and / or promoting differentiation of chicken myoblasts. The detection kit provided by the application can be used for detecting

The invention discloses an application of a KLF9 gene in chicken heat stress resistance breeding. The invention also discloses an interference fragment of the KLF9 gene, a primer pair based on the interference fragment of the KLF9 gene, an interference vector containing the interference fragment and a construction method of the interference vector. The invention also discloses a detection kit. The invention also discloses an interference fragment of the KLF9 gene, a primer pair based on the interference fragment of the KLF9 gene, and an application of the interference vector in chicken heat stress resistance breeding. The invention further discloses a breeding method of the heat stress resistant chicken. The gene KLF9 provided by the invention can be used as a marker for detecting the heat stress resistance level of the chicken, the expression quantity of the gene can be quickly judged by detecting the expression quantity of the gene in the skeletal muscle of the chicken in breeding, the chicken with a half-sib relationship with a detected individual can be selected and remained, and the quick breeding of target characters in the chicken breeding process can be accelerated. The primer pair and the kit provided by the invention can be used for detecting the expression quantity of the KLF9 in the chicken skeletal muscle tissue, and the detection method is simple and rapid.

The application discloses application of a GSDME gene SNP molecular marker in judgment of heat adaptability of dairy cows and belongs to the technical field of animal molecular breeding. The GSDME gene SNP molecular marker is chr4:71097215A>G, a bovine reference genome ARS-UCD2.0; the SNP molecular marker gene polymorphism is A or G, and can be used for judgment of heat adaptability of dairy cows. The chr4:71097215A>G is a missense mutation subjected to positive selection, and the mutation causes a missense mutation (p.Tyr43Cys) of a pore-forming domain at the N terminal of a GSDME protein. The heat tolerance of a short-hair Holstein cow individual with the genotype GG of the molecular marker is significantly higher than that of individuals with the genotypes AA and AG. The marker is derived from a Senepol cow introgression region and subjected to positive selection, the detection method is simple and fast, is suitable for screening of heat-tolerant individuals and gene editing breeding practices, and has a clear biological background and breeding value.

The application discloses a method for improving resistance to wheat scab QFhb-2DL and QFhb- 3BL The application discloses a method for improving resistance to wheat scab, a molecular marker, a primer, the molecular marker comprising a dCAPS marker of QFhb-2DL and / or a KASP marker of QFhb-3BL . The application first develops a molecular marker closely linked to QFhb-2DL and a molecular marker closely linked to QFhb-3BL . Under the premise of stabilizing the resistance to stripe rust, dwarf, large spike and multiple spike characteristics of a wheat variety in the upper reaches of the Yangtze River, the application realizes molecular design breeding by using the developed new marker closely linked to the locus, and quickly introduces the disease-resistant genotypes of QFhb-2DL and QFhb-3BL into the wheat variety in the upper reaches of the Yangtze River, breeds a strain which is resistant to scab and has a yield significantly higher than that of a control, and realizes a breakthrough in the resistance to scab in the upper reaches of the Yangtze River.

The present invention discloses a detection method and kit for catecholamines and catecholamine metabolites, including: using creatinine Cre correction, using dansyl chloride derivatization method to convert 8 catecholamines and catecholamine metabolites in urine samples into stable derivatives, using high performance liquid chromatography for separation, and using triple quadrupole tandem mass spectrometer to simultaneously determine the concentrations of creatinine and 8 catecholamines and catecholamine metabolites in urine and process data. The present invention selects random urine samples as detection objects, solves the drawbacks of poor compliance of clinical outpatients and difficulty in collecting samples, does not increase creatinine detection experiments, and 8 catecholamines and catecholamine metabolites react with dansyl chloride to generate stable derivatives, and then using triple quadrupole tandem mass spectrometer to simultaneously determine the concentrations of creatinine and 8 catecholamines and catecholamine metabolites in urine. The detection method of the present invention is simple, fast, accurate and efficient, and has high clinical application value.

The present invention provides a functional molecular marker for detecting wheat stem rust resistance gene Sr8155B1, a detection method and an application thereof. The functional molecular marker comprises: an upstream primer shown in SEQ ID NO: 1 and a downstream primer shown in SEQ ID NO: 2. The method can solve the problem in the prior art that it is difficult to accurately detect whether wheat stem rust resistance gene Sr8155B1 exists in plants, and is applicable to the field of molecular genetic breeding.

The invention relates to the technical field of virus detection, in particular to a real-time fluorescent PCR (polymerase chain reaction) primer probe composition, a kit and a method for detecting a rodent K virus. The primer probe composition comprises a primer and a TaqMan probe, the nucleotide sequence of a forward primer of the primer pair is as shown in SEQ ID NO.1, and the nucleotide sequence of a reverse primer of the primer pair is as shown in SEQ ID NO.2; the nucleotide sequence of the TaqMan probe is as shown in SEQ ID NO. 3. The primer probe composition is high in specificity and sensitivity, the detection method is simple, convenient and rapid, the detection time is short, the detection method is suitable for detection of a large number of sample experiment rodent K viruses, and an effective technical means is provided for rapid detection of the rodent K viruses.