248 results about "Quenching (fluorescence)" patented technology

The invention belongs to the technical field of battery health state monitoring, and relates to an unmanned aerial vehicle battery health state on-line prediction method comprising the following steps: exciting a quantum dot tracer agent in a battery monitoring area to obtain an initial fluorescence spectrum, and carrying out real-time comparison to identify fluorescence quenching caused by gas production and generate a preliminary aging signal; further analyzing the spectrum drift characteristics so as to analyze lithium aging and pure gas production; triggering ultrasonic detection based on a diagnosis result, and extracting time-frequency characteristics of acoustic echoes to construct quantitative acoustic fingerprints; and finally, through cross validation of optical and acoustic information, generating a comprehensive health status report containing a risk level and an aging type. According to the method, multi-dimensional on-line diagnosis and early warning on the early aging stage of the battery are realized, and the problems of hysteresis quality, difficulty in positioning and inaccurate risk assessment caused by dependence on macroscopic electrical parameters in a traditional method are solved.

The invention relates to the technical field of fluorescent hydrogel, in particular to high-strength and high-stability fluorescent hydrogel as well as a preparation method and application thereof, the fluorescent hydrogel is formed by physical crosslinking of a hydrogel matrix, a rare earth fluorescent complex and a stabilizer, the hydrogel matrix is of a double-network structure formed by physical crosslinking of polyvinyl alcohol and carboxymethyl cellulose; the rare earth fluorescent complex is formed by connecting rare earth metal ions and an organic antenna material through coordination; the stabilizer is trisodium citrate. Compared with other fluorescent hydrogel, the fluorescent hydrogel with high strength and high stability provided by the invention has the advantages that the phenomena of fluorescence decay and fluorescence quenching caused by metal ions and saturated salt solution are avoided, and the fluorescence is kept stable in a wide pH range. Because the fluorescence characteristic is not influenced by environmental corrosion factors, the information can be stably displayed for a long time, and the method can be used for information encryption and anti-counterfeiting in an extreme environment.

The invention provides a preparation method of nitrogen and phosphorus-doped carbon dots and application of the nitrogen and phosphorus-doped carbon dots in quercetin detection, the nitrogen and phosphorus-doped fluorescent carbon dots (N, P-CDs) are successfully constructed for the first time by adopting a hydrothermal synthesis method, and the carbon dots have emission peaks at 465 nm under the excitation wavelength of 325 nm and show green fluorescence under the irradiation of an ultraviolet lamp; quinine sulfate is used as a standard reference substance, and the quantum yield is 1301%; an N, P-CDs solution is used as a probe molecule, after quercetin is added, fluorescence quenching of N, P-CDs is induced, and the concentration of quercetin and the fluorescence intensity of N, P-CDs are linearly changed, so that the method can be used for quantitative detection of quercetin. The N, P-CDs preparation method provided by the invention is simple and rapid, the product property is stable and non-toxic, the detection sensitivity on the quercetin is high, and the N, P-CDs can be used for detecting the content of the quercetin in serum.

The invention provides a method for specifically detecting AFP based on a photoinduced electron transfer effect between blood orange RCDots and an AFP-aptamer, and the preparation and AFP detection principles of a near-infrared PET fluorescent probe for AFP detection are as follows: the RCDots with rich carboxyl groups on the surface can strongly adsorb ssDNA to the surface of the RCDots through pi-pi stacking interaction; and a formed RCDots / AFP-aptamer compound is subjected to a photo-induced electron transfer effect, so that the fluorescence of the RCDots is remarkably quenched. However, the affinity between the AFP and the AFP-aptamer is stronger than pi-pi stacking interaction, so that ssDNA is separated from the surface of the RCDots, the light-induced electron transfer effect disappears, the fluorescence of the RCDots is recovered, and the fluorescence intensity is changed along with the change of the concentration of the AFP, so that the concentration of the AFP is detected with one kind of specificity. The detection method is simple, has the characteristics of wide detection linear range, low detection limit, good specificity and the like, and can be widely applied to clinical detection of liver cancer markers.

The invention belongs to the technical field of talc, and provides a method for detecting fluorescent substances in talc raw ore, which comprises the following steps: S1, carrying out ball milling on the talc raw ore in a ball mill, sieving to obtain sieved powder, and adding deionized water into the sieved powder to obtain slurry; s2, adding the slurry into the mixture to enable the pH value of the slurry to be 5, stirring, and standing in a water bath for 2-3 hours to obtain a first precipitate; s3, adding a thiourea-ascorbic acid solution into the first precipitate to obtain a second precipitate; and S4, carrying out secondary ball milling on the second precipitate, and spreading the second precipitate in an enamel plate to detect whether the talc raw ore contains the fluorescent substances or not. The hydrochloric acid can dissolve quartz and mica to avoid generation of background fluorescence or quenching effect, and the nonionic surfactant polyethylene glycol can be adsorbed on the surface of the mineral to reduce interfacial tension, so that the hydrochloric acid is easier to infiltrate tiny pores, the pit defect caused by local over-corrosion is reduced, the pickling uniformity is improved, and the detection sensitivity is improved.

The invention relates to an automatic fluorescence quenching system and method. A sample storage unit is arranged on a rack. And the reaction quenching unit is arranged on the rack and is positioned on one side of the sample storage unit. The carrying unit is arranged on the rack, and the moving range covers the sample storage unit and the reaction quenching unit. And the sample reinspection unit is arranged on the rack and is positioned on the other side of the reaction quenching unit. And the sample recovery unit is arranged on the rack and is used for storing the biological samples which are qualified in quenching. The reagent unit is arranged in the rack and is connected with the quenching box of the reaction quenching unit through a liquid pipeline. The controller is in communication connection with the sample storage unit, the reaction quenching unit, the carrying unit, the sample reinspection unit, the reagent unit and the sample recovery unit. According to the automatic fluorescence quenching system and method, the problems of subjectivity and inconsistency due to the fact that a traditional method depends on manual observation and judgment are solved, the quenching effect of output samples is more stable, and the requirement for result consistency in high-throughput research is met.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The application belongs to the field of explosive detection, and discloses a polyimide loaded triaminophthalocyanine probe, a preparation method thereof and application thereof in detecting nitroaromatic explosives. The probe has the following general structure formula. The probe is prepared by cross-linking copolymerization of 5,10,15-tri(4-aminophenyl)-20-phenyl porphyrin, aromatic tetracid dianhydride and aromatic organic diamine. The polyimide high-molecular cross-linked branch loaded triaminophthalocyanine compound with fluorescent light emitting performance is prepared for the first time. The probe is applied to detecting various nitroaromatic explosives by quenching fluorescence response, and the detection method is simple, and has good application prospect in the field of public security.

The invention discloses a TADF luminescent material and a preparation method thereof, and relates to the technical field of organic optoelectronics, the molecular structure of the material comprises a phenyl triazine derivative electron-deficient core as an electron acceptor and a carbazole or tert-butyl carbazole electron-rich group as an electron donor; an aggregation-induced emission mechanism is introduced into the thermally activated delayed fluorescence material, so that the aggregation-induced quenching problem of a traditional thermally activated delayed fluorescence material and the exciton annihilation effect under high brightness are effectively solved, and meanwhile, the defect that a traditional aggregation-induced emission molecule is low in electroluminescence efficiency is overcome; the material can be used for preparing a non-doped organic electroluminescent device, the process is simplified, the cost is reduced, and the material has application potential in the field of intelligent response materials.

This invention provides a fluorescent labeling and conjugation method that exhibits enhanced signal intensity. [Solution] This disclosure relates to the field of fluorescent dyes, and more particularly to compositions and methods for increasing fluorescence signals and decreasing fluorescence quenching.

The invention relates to the technical field of smart agriculture, and discloses a spectrum feed-forward greenhouse control system based on physiological vector Pareto optimization, comprising an intelligent sensing module for collecting crop pulse modulation fluorescence parameters, spectrum fingerprints and environmental parameters; the model decision-making module is coupled with the three algorithm units, the long-period unit is used for outputting an optimal allowable interval of environment regulation and control based on a crop model, the short-period unit is used for predicting physiological risks and outputting dynamic regulation weights based on spectral feedforward, and the real-time unit is used for constructing physiological vectors composed of actual photochemical quantum yield and non-photochemical quenching; executing Pareto optimization under interval constraint and weight guidance, balancing light energy utilization efficiency and physiological stress, and generating an accurate control instruction; the intelligent execution module regulates and controls the environment; and the online calibration module is used for recording data to update the crop model and the prediction model online. According to the method, long-term energy efficiency planning and instantaneous physiological optimization are coordinated, and the precision, prospect and adaptive capacity of greenhouse control are improved.

The invention belongs to the technical field of chrysin detection, and particularly relates to a dual-emission fluorescent molecularly imprinted polymer as well as a preparation method and application thereof. The method comprises the following steps: preparing carbon dots with different emission wavelengths; carrying out amination modification on the carbon dots; and preparing the dual-emission fluorescent molecularly imprinted polymer. The method also comprises the following steps: respectively adding the dual-emission fluorescent molecularly imprinted polymer into chrysin solutions with different known concentrations, carrying out fluorescence quenching reaction, and establishing a standard curve according to the linear relationship between the detected fluorescence intensity and the corresponding concentration of the chrysin solution; adding the dual-emission fluorescent molecularly imprinted polymer into a solution of a to-be-detected product, and carrying out fluorescence quenching reaction to obtain fluorescence intensity; and calculating the concentration of chrysin in the solution to be detected according to the standard curve. The preparation method is used for solving the technical problems that in the prior art, a single-emission fluorescent molecularly imprinted polymer has defects in the aspects of sensitivity, background fluorescence interference resistance in a complex environment and the like in the detection process.

The invention discloses a biosensor based on an ultraviolet light triggered bio-barcode release technology (OBCA) and construction and application of the biosensor. The biosensor comprises nucleic acid functionalized silver nanoparticles, nucleic acid functionalized magnetic beads and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a detection system, the DNA bar code modified on the surface of the silver nanoparticle contains a light cleavable linker, a CRISPR / Cas12a activation sequence and a target capture sequence I, and a DNA capture probe containing a target capture sequence II is fixed on the surface of the magnetic bead through biotin-streptavidin action. During detection, target miRNA mediates two capture sequences to form a sandwich compound, after magnetic separation and thermal denaturation elution, 365nm ultraviolet radiation releases DNA bar code fragments, and the Cas12a trans-cleavage fluorescence quenching reporter probe is activated to generate a signal. According to the method, nucleic acid amplification and reverse transcription are not needed, the detection limits of miR-21, miR-155 and mi-210 respectively reach 158.94 aM, 763.56 aM and 197.82 aM, single-base mismatch and precursor miRNA can be distinguished, the serum sample detection result is highly consistent with that of RT-PCR, and the method is suitable for high-sensitivity quantitative detection of miRNA.

The invention relates to a method for specifically detecting tetrahydrocannabinol and hydride thereof by utilizing bridged beta-cyclodextrin, and belongs to the technical field of analysis and detection of supramolecular materials. The method comprises the following steps: firstly, combining 1, 6-hexamethylenediamine bridged bis-beta-cyclodextrin with an indicator to form a supramolecular probe; in a buffer system with a pH value of 9-11, tetrahydrocannabinol or a hydride thereof specifically competes to be combined with a cavity and displaces an indicator by utilizing a synergistic inclusion effect of a bridging subject and dissociation difference of guest molecules, so that the system is subjected to fluorescence quenching or color change, and a structural analogue cannabidiol does not generate obvious interference. The method solves the problems that the sensitivity is low and cannabidiol cannot be effectively distinguished in the prior art through cooperation of specific main body construction and environment regulation, has the advantages of being rapid in detection, high in specificity, high in accuracy and the like, and is suitable for environment monitoring and field screening.

The application discloses a double-line ratio fluorescent competitive immunochromatographic detection device and a preparation method and application thereof, and relates to the technical field of immunochromatographic detection.The detection device comprises a double-specificity recognition unit mixed-labeled quenched fluorescent probe, a background fluorescent material and a solid phase substrate, the solid phase substrate is provided with a T line on which a target object binding ligand is immobilized and a C line on which a quality control ligand is immobilized; and the application also provides application of the detection device in detection of exogenous harmful substances.The application solves the technical problem of low detection sensitivity of the existing method, and adjusts the binding amount of the probe on the T line and the C line through an immune competition reaction, adjusts the quenching degree of the background fluorescence based on an internal filter effect, takes (T line quenched fluorescent signal / background fluorescent signal) / (C line quenched fluorescent signal / background fluorescent signal) as a quantitative signal, and takes the C line to participate in ratio calculation to improve the detection sensitivity, so that the application is suitable for high-sensitivity qualitative and quantitative detection of small molecule compounds and the detection sensitivity is improved.

The invention relates to the technical field of photovoltaic modules, and particularly discloses a tetraphenyl ethylene-benzotriazole derivative compound and application thereof in the photovoltaic field. The absorption spectrum of the tetraphenyl ethylene-benzotriazole derivative compound disclosed by the invention can be subjected to red shift to 420 nm, so that high-energy ultraviolet light can be completely absorbed; the maximum emission wavelength of a fluorescence emission spectrum is more than 440nm and even can reach 500nm, so that the light utilization level of the crystalline silicon solar cell is improved; the absolute light-emitting quantum efficiency is very high, and the converted wavelength can be better utilized by a photovoltaic device. The substituted tetraphenyl ethylene part contained in the derivative compound has AIE performance, and the fluorescence quenching effect of traditional small organic molecules in high concentration, solid state and aggregation state is avoided. The light conversion film can overcome the problem that the fluorescence quantum yield is remarkably reduced in the laminating process, the performance of the film is not affected when the phenomena of precipitation, agglomeration and the like of auxiliaries in the film occur, and the light conversion film is used for preparing a photovoltaic module.

This invention belongs to the field of heavy metal ion detection technology, specifically relating to a method for detecting Co based on microwave-synthesized histidine carbon quantum dots from yam. 2+ The method involves adding ground yam powder and histidine to deionized water, stirring, and then microwaving. After centrifugation, the supernatant is filtered through a filter membrane, and the filtrate is the yam-doped histidine carbon quantum dot (F-CDs) mother liquor. Different concentration gradients of Co are added to the F-CDs mother liquor. 2+ Subsequently, the fluorescence intensity of the F-CDs mother liquor increased with Co. 2+ The quenching efficiency F / F0 and Co gradually decrease with increasing concentration. 2+ In 1×10 ‑6 ~9×10 ‑6 The method exhibits good linearity within the mol / L concentration range and demonstrates good selectivity, indicating that it is effective for Co. 2+ The detection is characterized by its sensitivity, speed, and high selectivity.

The application discloses a high-brightness quantum dot fluorescent microsphere and a preparation method thereof based on an alkali catalysis strategy, and comprises the following steps: (1) preparing thiol-modified dendritic mesoporous silica microspheres; (2) assembling the thiol-modified dendritic mesoporous silica microspheres and quantum dots to obtain a dendritic silica / quantum dot assembly; (3) adding 3-mercaptopropyl triethoxysilane to the dendritic silica / quantum dot assembly, and performing organic silane modification on the dendritic silica / quantum dot assembly in a non-polar organic solvent by taking an organic alkali as an alkali catalyst; and (4) growing a silica shell layer on the surface of the organic silane-modified dendritic silica / quantum dot assembly by a method to obtain the high-brightness quantum dot fluorescent microsphere. The preparation method avoids fluorescence quenching of oil-phase quantum dots in the process of coating silica, and completely retains excellent optical properties of the oil-phase quantum dots.

The invention relates to the technical field of food hazard factor detection, in particular to a food hazard factor detection method and system based on biomimetic hydrolase catalytic fluorescence generation. Comprising the following steps: S1, obtaining a turbidity characteristic value, background fluorescence intensity and a fluorescence quenching coefficient of a to-be-detected sample solution; s2, based on the turbidity characteristic value and the fluorescence quenching coefficient, calculating to obtain a robustness index; s3, according to a comparison result of the robustness index and a preset qualified threshold value, through a preset closed-loop feedback control logic, determining a working gain used for total fluorescence intensity signal acquisition; s4, performing dynamic signal correction processing including gain normalization to obtain a normalized apparent fluorescence signal; s5, based on the normalized apparent fluorescence signal, calculating to obtain an apparent catalytic rate; and S6, determining the concentration of the target hazard factor according to the apparent catalytic rate, the turbidity characteristic value and the fluorescence quenching coefficient. According to the method, the accuracy and the reliability of detecting the target hazard factors in the complex matrix are remarkably improved.

The invention provides a light-emitting auxiliary material and a preparation method and application thereof, and belongs to the technical field of light-emitting materials, the structural formula of the light-emitting auxiliary material is shown in the general formula I. The light-emitting auxiliary material takes 9, 9-phenylalkyl fluorene as a parent nucleus, aryl and arylamine groups are connected to the two sides of fluorene of the 9, 9-phenylalkyl fluorene respectively, 9-phenylalkyl fluorene has relatively high glass transition temperature and quantum fluorescence efficiency, the stability and luminous efficiency of the compound can be effectively improved, the energy levels of the highest occupied molecular orbital and the lowest unoccupied molecular orbital can be flexibly adjusted by introducing an electron-donating group arylamine, and the energy barrier of hole injection can be effectively reduced; meanwhile, the other side is connected with an aryl group, so that a conjugated system can be effectively extended, a fluorescence quenching phenomenon caused by intermolecular pi-pi accumulation and excessive molecular aggregation is reduced, the service life of an OLED (Organic Light Emitting Diode) device can be effectively prolonged, the luminous efficiency of the OLED device can be effectively improved, and the driving voltage can be reduced by utilizing the light-emitting auxiliary material.

The application belongs to the technical field of functional polyurethane materials, and more particularly relates to a polyurethane fluorescent material applicable to bacterial detection and a preparation method thereof. The polyurethane pre-polymer is obtained by pre-polymerization of a hydrophobic polyether monomer, isocyanate, a catalyst and a hydrophilic solvent; a chain extender is added to the pre-polymer for chain extension reaction, and then a capping agent is added for capping reaction, and after curing, a polyurethane elastomer is obtained; the polyurethane elastomer is mixed with an ethanol solution, curcumin is added, and stirred uniformly to obtain a fluorescent polyurethane solution, and curing obtains a polyurethane-based fluorescent material. The polyurethane fluorescent material applicable to bacterial detection is designed through a polyurethane segment, ensures solid-state light emission of curcumin, does not cause fluorescence quenching, ensures activity of a heat-sensitive base material at room temperature, and the fluorescent color is different under different bacterial concentrations, and the response is sensitive.

The invention discloses a nitrogen-doped carbon dot and a rapid fluorescence detection method thereof for indoxacarb residue, and belongs to the field of biosensor technology and food quality detection. L-theanine and ethidene diamine are used as raw materials, the nitrogen-doped carbon dots are prepared through a hydrothermal method, the N-CDs have the advantages of being stable in fluorescence emission, good in salt tolerance and excellent in photobleaching resistance, and indoxacarb can specifically quench fluorescence through the static quenching effect. By optimizing N-CDs synthesis conditions and indoxacarb detection conditions, an indoxacarb fluorescence sensing detection system with high selectivity and high sensitivity is constructed. The detection method disclosed by the invention can be suitable for detecting indoxacarb residues in environmental water samples such as tap water and river water and agricultural product matrixes such as apples and tea leaves, the standard recovery rate is good, the relative standard deviation is low, on-site rapid screening of the indoxacarb residues can be realized, and a new technical means is provided for monitoring the quality safety of the environment and agricultural products.

The invention belongs to the technical field of digital pathology and fluorescence imaging, and relates to a fluorescence pathological section scanning system and a scanning method thereof. The system comprises a fluorescence imaging module, a scanning displacement table for bearing and driving a pathological section to perform two-dimensional scanning under a microscope objective, and a protective gas supply module comprising a protective gas storage or generation device, a gas pipeline and a protective gas nozzle. The protective gas spray head is arranged above the scanning displacement table and located near the optical axis of the microscope objective, protective gas with stable chemical properties is continuously sprayed out towards the focus area of the microscope objective in the scanning process, a low-oxygen-content area is formed on the surface of a section, photooxidation quenching or bleaching of fluorescent dye is effectively inhibited, and the service life of the section is prolonged. And the brightness uniformity and the quantitative analysis reliability of the fluorescence image under long-time and multi-view splicing scanning are improved. The invention further discloses a corresponding single-piece and multi-piece continuous fluorescent pathological section scanning method.

The invention belongs to the technical field of fluorescent dyes, and particularly relates to a phenanthroline-based double-BODIPY fluorescent dye with an asymmetric structure as well as a preparation method and application of the phenanthroline-based double-BODIPY fluorescent dye. The preparation method comprises the following steps: mixing a chlorinated BODIPY derivative with 1, 10-phenanthroline-5-amino, adding palladium acetate, tri-tert-butylphosphine, potassium tert-butoxide and toluene, and carrying out a heating reflux reaction; and after the reaction is finished, cooling, reducing pressure to remove toluene, dissolving the obtained product with dichloromethane, washing, drying, concentrating, and separating and purifying through silica gel column chromatography to obtain the target dye. The unique asymmetric structure in the prepared target dye destroys the planarity and symmetry of molecules, effectively weakens the fluorescence quenching effect caused by molecular self-absorption and pi... pi accumulation, and further has relatively large Stokes shift and high solid-state luminous efficiency; good application potentials are shown in a plurality of fields such as high-performance solid-state light-emitting devices and biological probes.

The invention relates to application of a Fe < 3 + >-ATP-CK cascade regulation type carbon dot fluorescent probe in dynamic monitoring of creatine kinase content, and relates to the field of new application of carbon dots. Fluorescence of B, N-CDs is quenched by Fe < 3 + >, and then fluorescence is recovered after ATP is added through specific binding of Fe < 3 + > and ATP. When CK catalyzes ATP to react with Cr, fluorescence is quenched again due to reduction of the ATP content. On the basis of high-selectivity competitive combination of Fe < 3 + > and ATP, interference of ADP, AMP and other analogues can be eliminated. The multiple verification mechanisms of Fe < 3 + > quenching, ATP recovery and CK re-quenching significantly reduce the false positive result, and enhance the detection reliability. A portable sensing platform constructed based on a smart phone establishes a new method for bedside detection of CK, establishes a brand new strategy for CK detection, and shows a wide prospect in rapid screening application of clinical diagnosis of myocardial infarction and the like.

The invention discloses a fluorescence micromanipulation device for preventing quenching of a fluorescence sample, and relates to the technical field of microscopes, the fluorescence micromanipulation device comprises a stereoscopic microscope body, and the bottom of the stereoscopic microscope body is provided with a height-adjustable base; the bearing hole is formed in the base, and a carrying plate made of a transparent material is mounted in the bearing hole; the anti-quenching operation cabin is detachably mounted on the base, and the cabin wall of the anti-quenching operation cabin is made of a lightproof material and is a hollow frame body; according to the anti-quenching stereoscopic microscope, a controllable operation space with local shading is constructed on the stereoscopic microscope, so that an operator can simultaneously realize effective anti-quenching of a fluorescent sample and sufficient illumination required by fine operation in a bright conventional experiment environment; and the efficiency and the precision of long-time micromanipulation are improved.

The invention relates to a preparation method of a biosensor, in particular to a construction method of an acetylcholin esterase (at) amorphous covalent organic framework (AChE (at) aCOFs-DB) photoenzyme integrated biosensor, and belongs to the technical field of biosensors. According to the method, an in-situ growth mineralization strategy is adopted, effective packaging of AChE in aCOFs-DB is achieved, the fluorescence performance of aCOFs is enhanced through a hydrogen bond auxiliary intramolecular rotation limitation design, and meanwhile the structural stability and catalytic activity of AChE are improved; the biosensor with high sensitivity and high selectivity is constructed based on the synergistic effect of fluorescence characteristics and enzyme catalytic performance; the sensor can be used for sensitive detection of organophosphorus pesticides such as chlorpyrifos, and the detection limit can reach a nanogram / milliliter level; the detection principle is as follows: AChE catalyzes a substrate to generate a product, and fluorescence quenching is caused by an inner filtering effect; when the organophosphorus pesticide (such as chlorpyrifos) exists, the AChE activity is inhibited, the catalytic capability is reduced, and a fluorescence signal is recovered, so that quantitative detection of the pesticide is realized; the preparation method is simple and convenient to operate and controllable in structure, is suitable for rapid detection of organophosphorus pesticides in agricultural products and environmental samples, and has a good application prospect.

The invention discloses a fluorescent probe based on an ESIPT principle and application of the fluorescent probe in detection of total iron, ferrous iron and ferric iron. The probe OHNH contains a phenolic hydroxyl group, generates strong fluorescence through ESIPT, and has the advantages of good light stability, high fluorescence quantum yield, good water solubility, difficult hydrolysis, simple synthesis and the like. Fe and OHNH can generate specific complexation, the ESIPT process is influenced, and fluorescence quenching is caused. Fe is easily oxidized into Fe under the heating condition of a drying oven, and then fluorescence quenching is also triggered. Based on the principle, high-selectivity and high-sensitivity detection of total iron, ferrous iron and ferric iron can be realized. The method is low in cost, easy and convenient to operate, high in specificity and high in accuracy, and visual detection can be achieved. Furthermore, the method can be used for valence state distinguishing and content determination of iron in complex matrixes such as medicines, foods and water bodies, and has important practical application value.

1. The name of the design product: fluorescence quenching instrument. 2. The use of the design product: for background quenching, precise differential fluorescence quenching, medical biological detection instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

Provided is a method for generating a Raman spectrum with a high SN ratio using spectral data in a quenching process, wherein the Raman spectrum is generated by separating Raman scattered light from fluorescence by a method free of arbitrariness.　The method for generating a Raman spectrum comprises: a step for repeating a procedure in which a sample is irradiated with laser light, Raman scattered light that is scattered from the sample and fluorescence that is radiated from the sample are received, and a spectrum that contains the Raman scattered light and the fluorescence is obtained, and recording a first spectrum, a second spectrum, ..., and an nth spectrum; a step for determining, for each of the n recorded spectra, the intensity of the Raman scattered light as the product of an exposure time and the power of the laser light with which the sample is irradiated; a step for determining the intensity of the fluorescence for each of the n recorded spectra; and a step for using the intensity of the determined Raman scattered light and the intensity of the determined fluorescence to determine a Raman spectrum by the least squares method.