722 results about "Quenching (fluorescence)" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention belongs to the field of miRNA detection, and particularly relates to a non-diagnostic-purpose miRNA detection method based on dual cascade amplification of a Pt nanoprobe and a kit. Comprising the following steps: 1) obtaining a Pt NPs (at) Ab nanoprobe; (2) carrying out an ISAR-CRISPR / Cas12a reaction; and (3) dripping on a nucleic acid test strip for capillary action, adding a quenching substrate into the T line for catalysis after the chromatographic reaction is finished, and judging the concentration of miRNA by observing the T line. According to the invention, a Pt nanoprobe is introduced as a fluorescence quenching probe, and after TMB is dropwise added, double-amplification cascade fluorescence quenching is generated. Due to the adoption of a double-amplification cascade fluorescence quenching strategy, the fluorescence quenching efficiency can be greatly improved, so that an ultra-low background fluorescence signal is ensured. The method has a good application prospect, can be used as a general detection method for detecting a specific nucleic acid target, and is widely applied to the fields of nucleic acid detection and the like.

The invention provides a two-dimensional lanthanide series metal-organic framework material as well as a preparation method and application thereof, belongs to the technical field of detection material preparation, and aims to obtain a detection method which is relatively high in sensitivity, simple and convenient to operate and short in reaction time. The method comprises the following steps: firstly, synthesizing a two-dimensional lanthanide series metal-organic framework crystal under a solvothermal reaction condition, detecting different nitro aromatic explosives including TNP by taking the two-dimensional lanthanide series metal-organic framework as a fluorescent probe, and showing a characteristic emission peak of europium ions by the crystal; the crystal can be used as a fluorescent probe for detecting different nitro aromatic explosives, and the result shows that the crystal has fluorescence spectrums with different intensities for different detected objects; the crystal can sensitively, selectively and cyclically detect TNP through fluorescence quenching, and shows excellent anti-interference capability in the detection process, so that the crystal is an environment-friendly and efficient sensing agent for trace detection of TNP.

The invention relates to the technical field of biosensing, in particular to a method for synergistically activating a CRISPR / Cas12a system based on a T-type structure and application of the method, the CRISPR / Cas12a system can be activated by utilizing two split DNA activation chains and crRNA to form the T-type structure, the activated CRISPR / Cas12a system has trans-cleavage activity, an FQ-ssDNA probe modified with an FAM fluorophore and a BHQ1 quenching group can be subjected to trans-cleavage, a fluorescence signal is recovered, and the fluorescence signal is recovered. Through reasonable design of sources of activation chains corresponding to different targets, detection targets can be expanded from traditional nucleic acid detection to non-nucleic acid targets such as proteins and metal ions, so that biosensing application of multiple targets is realized, in addition, design based on a T-type CRISPR / Cas12a synergistic activation strategy has great flexibility and universality, and the application prospect is wide. The method can be used together with various amplification strategies and detection platforms, and the sensitivity of the analysis method is effectively improved.

The invention provides a D-A type organic fluorescent small molecule, a preparation method and application of the D-A type organic fluorescent small molecule in detection of nitrofuran antibiotics, and belongs to the technical field of fluorescent sensing. The D-A type organic fluorescent micromolecule is composed of 2-(2-aminophenyl) benzothiazole and an electron-donating group, the excitation spectrum of the D-A type organic fluorescent micromolecule is obviously overlapped with the absorption spectrum of nitrofuran antibiotics, and fluorescence quenching response can be shown through the inner filter effect; the trace nitrofuran antibiotics can be detected in a liquid phase in an ultrafast, high-sensitivity, excellent-selectivity and strong-anti-interference manner. According to the invention, a quantitative evaluation system can be constructed in combination with a smart phone, the RGB value of the image of the liquid-phase fluorescence sensor is output in real time, and rapid, accurate and visual quantitative detection of nitrofuran antibiotics in a real food sample or a water source is realized. In addition, the filter paper fluorescence sensor has the advantages of being portable and easy to prepare, and nitrofuran antibiotics can be rapidly identified on site in an actual scene.

The invention provides a probe capable of being used for fluorescence detection and RAA-LFD detection and application of the probe, the probe is used for isothermal amplification, a fluorophore, two quenching groups and tetrahydrofuran are arranged on the probe, and the fluorophore is located at the fifth end of a probe sequence; the second quenching group is positioned at the end 3 of the probe sequence; the first quenching group is located between the fluorophore and the second quenching group and is 12-20 basic groups away from the fluorophore; tetrahydrofuran is located between the fluorophore and the first quenching group and is 10-18 basic groups away from the fluorophore; the total length of the probe sequence is 27-32 basic groups, and compared with an existing RAA or RPA probe, the total length of the probe sequence is obviously shortened, design and use can be more convenient, the probe sequence is particularly suitable for RAA or RPA multiple amplification, multiple amplification of more than three times can be achieved, the probe can be used as a probe and a primer, the probe can be used for preparing a universal probe and a probe of an RAA-LFD system, and the probe sequence can be used for preparing the RAA-LFD system. The detection accuracy can be obviously improved, and the application prospect is wide.

The invention discloses a near-infrared two-region activatable probe as well as a preparation method and application thereof. The near-infrared second-region activatable probe comprises a near-infrared second-region fluorescent light-emitting unit and an analyte specific response unit; the near-infrared second-region light-emitting unit comprises a hemicyanine fluorophore structure; when the analyte specific response unit is chemically coupled with the near-infrared second-region light-emitting unit, the intramolecular charge transfer process of the light-emitting unit can be inhibited, and fluorescence quenching is caused; the analyte specific response unit is separated from the light-emitting unit after being subjected to specific reaction with an analyte, so that fluorescence is activated. The near-infrared two-region hemicyanine fluorophore selected by the near-infrared two-region activatable probe has higher fluorescence quantum efficiency, and the near-infrared two-region probe which can be activated by a specific analyte and has a high on-off ratio can be constructed by introducing an analyte specific response unit, so that high-temporal-spatial-resolution fluorescence detection of deep tissues can be realized; the method has a wide application prospect in the field of biomedical detection.

The invention belongs to the technical field of battery health state monitoring, and relates to an unmanned aerial vehicle battery health state on-line prediction method comprising the following steps: exciting a quantum dot tracer agent in a battery monitoring area to obtain an initial fluorescence spectrum, and carrying out real-time comparison to identify fluorescence quenching caused by gas production and generate a preliminary aging signal; further analyzing the spectrum drift characteristics so as to analyze lithium aging and pure gas production; triggering ultrasonic detection based on a diagnosis result, and extracting time-frequency characteristics of acoustic echoes to construct quantitative acoustic fingerprints; and finally, through cross validation of optical and acoustic information, generating a comprehensive health status report containing a risk level and an aging type. According to the method, multi-dimensional on-line diagnosis and early warning on the early aging stage of the battery are realized, and the problems of hysteresis quality, difficulty in positioning and inaccurate risk assessment caused by dependence on macroscopic electrical parameters in a traditional method are solved.

In a case where the target (30) is not supplied, binding through a nucleic acid sequence from the base end on a side of the solid phase surface of the complementary sequence complementary to a part of the nucleic acid sequence of the detection sequence to the other base end is maintained, which causes fluorescence of the fluorescent molecule (11) to be quenched by the quenching molecule (21) close to the fluorescent molecule (11). In a case where the target (30) is supplied, the target is bound to the detection sequence and the binding through the complementary sequence is released, which causes the fluorescent molecule (11) to separate from the quenching molecule (21) and emit fluorescence.

The invention relates to the technical field of fluorescent hydrogel, in particular to high-strength and high-stability fluorescent hydrogel as well as a preparation method and application thereof, the fluorescent hydrogel is formed by physical crosslinking of a hydrogel matrix, a rare earth fluorescent complex and a stabilizer, the hydrogel matrix is of a double-network structure formed by physical crosslinking of polyvinyl alcohol and carboxymethyl cellulose; the rare earth fluorescent complex is formed by connecting rare earth metal ions and an organic antenna material through coordination; the stabilizer is trisodium citrate. Compared with other fluorescent hydrogel, the fluorescent hydrogel with high strength and high stability provided by the invention has the advantages that the phenomena of fluorescence decay and fluorescence quenching caused by metal ions and saturated salt solution are avoided, and the fluorescence is kept stable in a wide pH range. Because the fluorescence characteristic is not influenced by environmental corrosion factors, the information can be stably displayed for a long time, and the method can be used for information encryption and anti-counterfeiting in an extreme environment.

The invention develops a self-driving and self-catalyzing method for detecting microRNA-21 (miR-21) on the basis of a three-legged DNA (deoxyribonucleic acid) nano machine. If a sample to be detected contains miR-21, the miR-21 can open an arm 1 of the three-legged DNA nano machine through a proximity effect, and the arm 1 and a nearby arm 2 form a DNA enzyme structure. The DNA enzyme can cut a substrate chain on the arm 2, the DNA arm 2 exposed after cutting is dissociated to compete with the arm 3, and a DNA fragment (sequence Q) marked with a quenching group is generated to leave the DNA nano-machine, so that a fluorescence signal is recovered. Meanwhile, the released sequence Q can catalyze the next self-driven and self-catalyzed circular reaction of the three-legged DNA nano machine. The component with close space positioning is constructed on a single DNA nanostructure, so that the local reactant concentration and the reaction rate are remarkably improved. And the fluorescence signal and the miR-21 have a linear relationship between the concentration of 10 pM and 50 nM. Compared with a free diffusion reactant, the effective concentration of the reactant is greatly increased. Therefore, the incubation time is obviously shortened to 35 minutes. The strategy has important significance in the aspect of providing reference value for miRNA detection and disease diagnosis.

The invention discloses a method for detecting L-cysteine in serum based on N, S co-doped carbon nanodots, and belongs to the field of analysis and detection. The method comprises the following steps: (1) preparing carbon dots by taking beta-mercaptoethylamine and p-phenylenediamine as precursors; (2) after Fe < 3 + > is added into the carbon dot solution, a carbon dot fluorescence quenching phenomenon occurs, and a carbon dot-Fe < 3 + > mixed system is obtained; then adding L-cysteine into the carbon dot-Fe < 3 + > mixed system to gradually recover the fluorescence of the system; and (3) constructing a linear relationship between the concentration of the L-cysteine and the fluorescence recovery degree to obtain the quantitative detection model for the L-cysteine in the serum sample. According to the method, the carbon dots are prepared by taking beta-mercaptoethylamine and p-phenylenediamine as precursors for the first time, quantitative detection of L-cysteine is realized, and the method is low in cost, high in sensitivity, suitable for field rapid detection and relatively high in practical value and popularization prospect.

The invention discloses a fluorescence-colorimetric bimodal nanoprobe and a biosensor for CAT detection, and relates to the technical field of nano biological materials, the fluorescence-colorimetric bimodal nanoprobe comprises UCNPs, hydrogen peroxide, ferrous ions and neutral red; the preparation method comprises the following steps: firstly, preparing UCNPs through a specific method, and then constructing the fluorescent-colorimetric bimodal nanoprobe by utilizing interaction of hydrogen peroxide, ferrous ions, neutral red and the UCNPs; in a fluorescence mode, the activity of CAT is reflected by measuring the quenching degree of an up-conversion fluorescence signal; in the colorimetric mode, the CAT activity is detected by observing the color fading degree of neutral red; the bimodal detection method not only improves the accuracy and sensitivity of detection, but also realizes visual rapid detection of CAT activity.

The invention provides a preparation method of nitrogen and phosphorus-doped carbon dots and application of the nitrogen and phosphorus-doped carbon dots in quercetin detection, the nitrogen and phosphorus-doped fluorescent carbon dots (N, P-CDs) are successfully constructed for the first time by adopting a hydrothermal synthesis method, and the carbon dots have emission peaks at 465 nm under the excitation wavelength of 325 nm and show green fluorescence under the irradiation of an ultraviolet lamp; quinine sulfate is used as a standard reference substance, and the quantum yield is 1301%; an N, P-CDs solution is used as a probe molecule, after quercetin is added, fluorescence quenching of N, P-CDs is induced, and the concentration of quercetin and the fluorescence intensity of N, P-CDs are linearly changed, so that the method can be used for quantitative detection of quercetin. The N, P-CDs preparation method provided by the invention is simple and rapid, the product property is stable and non-toxic, the detection sensitivity on the quercetin is high, and the N, P-CDs can be used for detecting the content of the quercetin in serum.

The invention discloses a sensing material for detecting atmospheric carboxylic acid, a preparation method, a detection method and application, belongs to the technical field of environmental functional materials, and solves the problems that an existing method is complex in operation and cannot realize real-time online monitoring, the sensing material for detecting atmospheric carboxylic acid is an MOF-802-EDTA-Pt composite film, and the preparation method comprises the following steps: taking 1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5- The preparation method comprises the following steps: synthesizing an MOF-802 material through a hydrothermal reaction by taking 1, 3, 5-benzene tricarbonic acid (BTC) and aluminum nitrate as raw materials and ethylene diamine tetraacetic acid (Na-EDTA) as a ligand; the preparation method comprises the following steps: dispersing MOF-802 in DMF to form a coating solution, and forming a film on a glass substrate through a pulling method; and then loading platinum nanoparticles, and carrying out solvothermal reaction and vacuum drying to obtain the composite sensing film. The MOF-802-EDTA-Pt composite film has a porous structure and fluorescence characteristics, and detection is realized through a fluorescence quenching effect of carboxylic acid molecules and an MOF skeleton. The method disclosed by the invention is controllable in process and high in material stability, still keeps excellent selectivity under the coexistence condition of interferents such as CO2 and NO2, and is suitable for real-time monitoring of carboxylic acid pollutants in the atmospheric environment.

The invention relates to the technical field of environment detection, in particular to a method for pre-enriching humic acid in a lake water body and determining molecular weight. The method comprises the following steps: (a) extracting and purifying humic acid in a lake water body; (b) preparing a lake water humic acid measured solution; (c) selecting a fluorescence peak position of a titration experiment; (d) fluorescence quenching titration of humic acid; and (e) calculating the molecular weight of humic acid. Tyrosine is used as a model compound and a humic acid standard sample for verification, and the accuracy and reliability of the method are proved.

The invention discloses a fixing reagent and method for double-color fluorescence detection of an anti-NMDAR antibody and application, the fixing reagent comprises paraformaldehyde and a mixture of methanol and acetone, and the mixture comprises 10-25% by volume of methanol, 75-90% by volume of acetone, 10-25% by volume of paraformaldehyde, 10-25% by volume of paraformaldehyde and 10-25% by volume of paraformaldehyde. The fixing effect is better by adopting paraformaldehyde with the mass-volume ratio concentration of 4% and a mixture of methanol with the volume fraction of 15% and acetone with the volume fraction of 85% in two steps. By adopting the fixing liquid and the fixing method, the antigenicity of NMDAR cannot be damaged, obvious green fluorescence cells can be seen in a positive sample, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed by using a fluorescence microscope.

The invention discloses a DNA silver nanocluster and a preparation method and application thereof, and belongs to the technical field of analytical chemistry. The method comprises the following steps: adding a template containing a DNA sequence and an AgNO3 solution into a PBS (Phosphate Buffer Solution), mixing, and carrying out a first dark reaction; then, adding a NaBH4 solution, and carrying out a second light-shielding reaction, so as to obtain the DNA-Ag NCs fluorescent probe; the DNA silver nano-cluster is applied to the determination of the cationic surface active agent; the fluorescence quenching effect is remarkable, the lowest detection limit can reach 2.0 mu M, the selectivity is good, common anionic surfactants, nonionic surfactants, metal ions in sewage and other interferents do not affect detection, and the accuracy and reliability of the method are improved.

The invention discloses a preparation method of photoresponse COFs coated CeO2 nano-enzyme, and relates to the field of nano-materials. According to the invention, FePc, TAPP and 2, 5-dihydroxy-1, 4-phthalaldehyde are used for creating a core-shell material CeO2 (at) FePC / por-COF of photoresponse peroxidase based on COFs (Covalent Organic Frameworks), and the core-shell material CeO2 (at) FePC / por-COF is used for preparing the photoresponse peroxidase based on COFs. According to the invention, the highly ordered structural characteristic of the COFs material is utilized, so that the problem of fluorescence quenching caused by aggregation of traditional micromolecule photosensitizers (such as porphyrin, phthalocyanine and the like) in the use process is effectively solved. According to the present invention, the photoresponsive peroxidase activity is significant, and the chromogenic substrate of tetramethyl benzidine (TMB) can be effectively catalyzed; fePc is integrated into a COFs structure, so that the photoresponse activity of the COFs under near-infrared 808 nm is enhanced, and the COFs have excellent reactive oxygen species (ROS) generation capacity, and have great potential in the aspects of photodynamic therapy, antibacterial treatment, cell imaging and the like in biomedical application.

The invention discloses a method for detecting 17 beta-estradiol by using a colorimetric-fluorescent dual-mode biosensor, and belongs to the technical field of chemical analysis and detection. According to the method, a colorimetric-fluorescent dual-mode biosensor for detecting 17 beta-estradiol is established based on oxidase-like activity and fluorescence quenching characteristics of manganese dioxide nanosheets and catalytic characteristics of alkaline phosphatase by taking a nucleic acid aptamer as a recognition molecule. The synthesis cost of the nucleic acid aptamer is low, the method has universality, and other target objects can be detected by changing the nucleic acid aptamer; mBs-DNA-ALP is prepared by using a biotin-streptavidin reaction and a DNA hybridization reaction, and a structure conversion process from double-stranded DNA to an aptamer / target compound is realized by using targeted induction, so that a detection signal is amplified; according to the method for detecting 17 beta-estradiol through colorimetric-fluorescence signals, dual-mode output is achieved, results are verified and supplemented mutually, and the specificity and accuracy of the method are improved.

The invention belongs to the technical field of fluorescent probes, and discloses a fluorescent probe based on BODIPY targeted lipid droplets and application of the fluorescent probe. The structural formula of the fluorescent probe for targeting the lipid droplets is as follows: # imgabs0 # is as follows: due to strong electron withdrawing capability and relatively large steric hindrance of ester groups, the ester groups of the probe can rotate in an excited state, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. The ester group of the probe can rotate in an excited state due to the strong electron withdrawing capability and large steric hindrance of the ester group, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. And due to the introduction of the ester group, the fat solubility of the BODIPY dye is further enhanced, so that the lipid droplet targeting property of the probe is effectively improved, and no-wash imaging of lipid droplets is realized.

The invention discloses a compound dye for fluorescent staining of cells in a complex matrix. The formula of the compound dye comprises a buffer system with the pH value of 6.0-8.0, a dye solution with the pH value of 6.0-8.0 and a dye solution with the total volume of 100 mL, the fluorescent dye is Sybr Gold 2 * (40 *) or 2 to 80 mg of propidium iodide; and 0.1 to 10 mL of triton X-100, and 0.1 to 10 mL of a 0 to 24 mL of glycerol; 0 to 800 mg of EDTA (Ethylene Diamine Tetraacetic Acid)-disodium; the invention relates to a polyamide-amine dendritic polymer. According to the two preferable kit compound fluorescent dye formulas, efficient dyeing of cells in a complex matrix and accurate counting of the number of the cells can be achieved, and the dye has excellent fluorescence developing capacity. Compared with other dyes, the compound dye has the advantages of high dyeing rate (dyeing can be carried out within 30 seconds), stable dyeing, long-time illumination and difficulty in fluorescence quenching.

The invention provides a method for specifically detecting AFP based on a photoinduced electron transfer effect between blood orange RCDots and an AFP-aptamer, and the preparation and AFP detection principles of a near-infrared PET fluorescent probe for AFP detection are as follows: the RCDots with rich carboxyl groups on the surface can strongly adsorb ssDNA to the surface of the RCDots through pi-pi stacking interaction; and a formed RCDots / AFP-aptamer compound is subjected to a photo-induced electron transfer effect, so that the fluorescence of the RCDots is remarkably quenched. However, the affinity between the AFP and the AFP-aptamer is stronger than pi-pi stacking interaction, so that ssDNA is separated from the surface of the RCDots, the light-induced electron transfer effect disappears, the fluorescence of the RCDots is recovered, and the fluorescence intensity is changed along with the change of the concentration of the AFP, so that the concentration of the AFP is detected with one kind of specificity. The detection method is simple, has the characteristics of wide detection linear range, low detection limit, good specificity and the like, and can be widely applied to clinical detection of liver cancer markers.

The invention discloses a fluorescent probe for determining the sol crystallinity of hydrotalcite colloid based on pi-pi accumulation regulated fluorescence and a preparation method of the fluorescent probe. According to the invention, an imidazole-anchored naphthalene diamine (IANA) fluorophore with a planar naphthyl motif is used as a fluorescent probe. Wherein an imidazole group has relatively strong binding capacity to transition divalent metal ions, and ordered arrangement of IANA is enhanced after transition metal and imidazole are coordinated, so that face-to-face pi-pi accumulation of a planar naphthyl motif is facilitated, absorption of ultraviolet light is inhibited, and fluorescence quenching is further caused. However, the internal structure orderliness and spatial atom arrangement of materials with different crystallinity degrees are different, so that the arrangement orderliness of IANA is different, the quenching degree of a probe is influenced, and the crystallinity degree can be measured through the quenching degree. According to the method, the sol crystallinity of the hydrotalcite colloid is determined through pi-pi accumulation regulated fluorescence, and a new way is opened up for exploring an alternative scheme of X-ray diffraction.

The invention provides an original staphylococcus aureus nucleic acid aptamer and a detailed screening and preparation process thereof, and aims to improve the detection accuracy and efficiency of common and dangerous strains. The specific DNA sequence (SEQ ID No: 1) of the aptamer is obtained through a series of complex bioinformatics and calculation processes, and comprises the following steps: carrying out a docking experiment on evolved oligonucleotide fragments by using molecular simulation software AutoDock, and calculating the affinity between each short-chain fragment and a target staphylococcus membrane protein FnBPA. Thirdly, the data are optimized through an ant colony algorithm, so that the sequence with the highest combination efficiency is identified; after the aptamer sequence with high affinity is obtained, the biosensor based on the aptamer is developed. The construction process of the sensor comprises the working procedures of mixing of activating reactants and preparation of the fluorescent probe and the quenching probe, and finally a sensitive and specific fluorescent quenching probe is formed. The change of the fluorescence emission wavelength and the ultraviolet absorption wavelength indicates that the aptamer is successfully fixed, so that a rapid and reliable method is provided for detecting the staphylococcus aureus. The method has very important application prospects in the fields of food safety monitoring, medical diagnosis and the like, the time required by means such as traditional microbial culture and the like can be remarkably shortened, and meanwhile, the cost selection lower than that of a molecular biological technology is provided.

The invention relates to an organic compound, a mixture, a composition, a light-emitting device and a display panel, the chemical structural formula of the organic compound is as shown in a formula I, and Ar in the formula I is selected from a group as shown in a formula II: # imgabs0 #. The organic compound provided by the invention has high fluorescence quantum efficiency and enough high stability; when the organic compound is used as a luminescent material, the problems of aggregation quenching, light color shift, low quantum efficiency and the like can be solved, so that the luminous efficiency of a luminescent device can be effectively improved, and the service life of the luminescent device can be effectively prolonged.

The invention discloses preparation of high-quantum-yield carbon dots based on machine learning guidance and detection application of a hydrogel kit of the high-quantum-yield carbon dots, and relates to the technical field of fluorescent nano material preparation and sensors. The method comprises the following steps: inputting reaction parameter data of synthesis of various carbon dots into a machine learning model, obtaining an optimal influence factor according to a prediction result, and realizing synthesis of the high-quantum-yield carbon dots. The carbon dot is in a sphere-like shape, the average particle size is 2.12 nm, the carbon dot has high fluorescence intensity, and an adopted precursor material is waste paper which is wide in source and low in cost. The synthesized high-quantum-yield carbon dots have the advantages of being rapid and sensitive in detection and the like, and mercury ions in Chinese herbal medicines can be rapidly detected according to fluorescence signal quenching. Meanwhile, the preparation of the carbon dot hydrogel kit can further realize colorimetric sensing, and the carbon dot hydrogel kit has good application value and prospect in the fields of environment, medicine, food and the like.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses a fluorescent dye for shoe soles as well as a preparation method and application of the fluorescent dye, and belongs to the technical field of fluorescent materials for shoes. The fluorescent dye has a structure as shown in a formula 1. The preparation method comprises the following steps: synthesizing an intermediate through Buchwald-Hartwisting arylation reaction, and carrying out Suzuki coupling to obtain a target product. The dye inhibits aggregation fluorescence quenching through a rigid plane structure and a deuterated group, the HOMO-LUMO energy gap is regulated to 2.8-3.2 eV, the dye has the broad band emission of 450-600 nm, and the fluorescence quantum efficiency reaches 0.55-0.75. When the brominated butyl rubber is applied to a sole formula, the brominated butyl rubber comprises the following components in parts by weight: 40-60 parts of brominated butyl rubber, 0.1-2 parts of fluorescent dye and the like, and a finished product is prepared by mixing, vulcanizing and foaming. Compared with a traditional dye, the fluorescence intensity is improved, the photooxidation resistance is enhanced, the dissolution rate is reduced by 60% or above, and the dye has excellent humidity and heat resistance and material compatibility.

The invention belongs to the technical field of ion detection, antibiotic detection and dye degradation, and relates to a zinc complex as well as a preparation method and application thereof. The chemical formula of the zinc complex is [Zn (NPTH) (4, 4 '-BMIBP). 3H2O], 4, 4'-BMIBP is 4, 4 '-bis (2-methylimidazolyl) biphenyl, and H2NPTH is 3-nitrophthalic acid. The zinc complex disclosed by the invention has a fluorescence recognition function on ferric ions, bivalent copper ions and furantoin and can be used for detecting the ferric ions, the bivalent copper ions and the furantoin in an aqueous solution, and the quenching constants are 4.91 * 10 < 3 >, 1.11 * 10 < 4 > and 7.22 * 10 < 3 > respectively. The degradation rate of the zinc complex disclosed by the invention on methylene blue within 130 minutes is 91.44%.