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Preparation method and application of single-particle double-emission ratiometric fluorescent probe

A ratiometric fluorescent probe and dual-emission technology, which is applied in the fields of chemistry and nanomaterials science, can solve the problems of cumbersome processing, expensive and bulky equipment, and time-consuming problems, and achieve the effect of simple operation, avoiding instability problems, and enriching color changes

Active Publication Date: 2019-09-03
中科合肥智慧农业谷有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, common detection methods mainly include high performance liquid chromatography, gas chromatography-mass spectrometry, etc., but these methods have certain disadvantages, such as cumbersome sample pretreatment process, expensive and cumbersome equipment, and long time-consuming, so simple and fast detection methods are urgently needed

Method used

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  • Preparation method and application of single-particle double-emission ratiometric fluorescent probe
  • Preparation method and application of single-particle double-emission ratiometric fluorescent probe
  • Preparation method and application of single-particle double-emission ratiometric fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of aminated blue carbon dots

[0044]Dissolve 0.6g of polyethyleneimine and 1g of citric acid in 30mL of ultrapure water, transfer to a 50mL reactor, react at 200°C for 6h, cool to room temperature, dialyze with a dialysis bag with a molecular weight cut-off of 1KDa for 48h, and then store In the refrigerator at 4°C.

[0045] 2. Preparation of red cadmium telluride quantum dots

[0046] 0.0638g tellurium powder and 0.10g NaBH 4 Mix in 4mL ultra-pure water, continuously feed nitrogen to maintain an oxygen-free environment; stir and react in an ice bath for 8 to 10 hours, the reaction solution gradually changes from black to pink to white, and NaHTe is generated in the upper layer; 0.2284g of chlorinated Dissolve cadmium and 210μL mercaptopropionic acid in 100mL ultrapure water, adjust the pH value of the solution to 9 with 1M NaOH, and then bubble nitrogen gas to remove the oxygen in the solution; inject 0.5-1M dilute sulfuric acid under anaerobic enviro...

Embodiment 2

[0057] 1. Preparation of aminated blue carbon dots

[0058] The preparation process of this step is the same as that of Example 1.

[0059] 2. Preparation of red cadmium telluride quantum dots

[0060] The preparation process of this step is the same as that of Example 1.

[0061] 3. Preparation of carboxylated silica nanoparticles embedded with red CdTe quantum dots

[0062] The preparation process of this step is the same as that of Example 1.

[0063] 4. Construction of ratiometric fluorescent probes

[0064] The preparation process of this step is the same as that of Example 1.

[0065] 5. Preparation of gold nanoparticles quenching ratio fluorescent probe

[0066] The preparation process of this step is the same as that of Example 1.

[0067] 6. The mixed system of fluorescence quenching is used to detect thiram

[0068] Add thiram solutions with concentrations of 0.04, 0.1, 0.4, 0.8, 1.0, 4.0, 8.0, and 10 μM to the mixed system of gold nanoparticles and ratio prob...

Embodiment 3

[0072] 1. Preparation of aminated blue carbon dots

[0073] The preparation process of this step is the same as that of Example 1.

[0074] 2. Preparation of red cadmium telluride quantum dots

[0075] The preparation process of this step is the same as that of Example 1.

[0076] 3. Preparation of carboxylated silica nanoparticles embedded with red CdTe quantum dots

[0077] The preparation process of this step is the same as that of Example 1.

[0078] 4. Construction of ratiometric fluorescent probes

[0079] The preparation process of this step is the same as that of Example 1.

[0080] 5. Preparation of gold nanoparticles quenching ratio fluorescent probe

[0081] The preparation process of this step is the same as that of Example 1.

[0082] 6. The mixed system of fluorescence quenching is used to detect thiram

[0083] Add thiram solutions with concentrations of 0.04, 0.1, 0.4, 0.8, 1.0, 4.0, 8.0, and 10 μM to the mixed system of gold nanoparticles and ratio prob...

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Abstract

The invention discloses a preparation method and application of a single-particle double-emission ratiometric fluorescent probe. Specifically, the preparation method of the single-particle double-emission ratiometric fluorescent probe includes: firstly preparing carboxylated silicon oxide with embedded red CdTe quantum dots, and then conducting surface covalent coupling of aminated blue carbon dots to construct the double-emission ratiometric fluorescent probe. The ratiometric fluorescent probe provided by the invention combines a fluorescence quenching system constructed by gold nanoparticlesand can be used for fluorescence enhanced detection of pesticide thiram, on the basis of fluorescence resonance energy transfer between gold nanoparticles and carbon dots, blue fluorescence can quench, red fluorescence silicon oxide is used as the internal standard, after adding of thiram, as the strong bonding effect of gold nanoparticles and sulfur atoms results brings about agglomeration, blue fluorescence recovers, and the process of closing and reopening of blue fluorescence can be realized, thus realizing the detection of thiram. The method has the advantages of high sensitivity, goodselectivity, strong anti-interference ability and low detection limit, and also can be used for detection of practical samples.

Description

technical field [0001] The invention relates to a preparation method and application of a single-particle dual-emission ratio fluorescent probe, which belongs to the field of chemistry and nanometer material science. Background technique [0002] Pesticide thiram, as a typical fungicide and preservative, is widely used in fruits, vegetables and crops to prevent various crops from pests and diseases, thereby improving their yield and quality. However, the abuse of thiram will lead to serious pesticide residue problems, causing drinking water and groundwater to be polluted, causing human beings to suffer from diseases on the skin and mucous membranes, and seriously affecting human body and physical and mental health. Therefore, how to quickly and quantitatively detect thiram is of great significance. [0003] At present, common detection methods mainly include high performance liquid chromatography, gas chromatography-mass spectrometry, etc., but these methods have certain di...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/88B82Y20/00B82Y30/00G01N21/64
CPCC09K11/025C09K11/883B82Y20/00B82Y30/00G01N21/643
Inventor 蒋长龙杨亮王振洋张淑东刘变化
Owner 中科合肥智慧农业谷有限责任公司
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