150 results about "Molecular fluorescence" patented technology

The invention provides a method and a system for evaluating the luminescence performance of a near-infrared two-region molecular fluorescent probe, and relates to the technical field of fluorescent probes, the method comprises the following steps: obtaining the initial configuration of the near-infrared two-region molecular fluorescent probe in an aqueous solution, and carrying out molecular dynamics simulation on the initial configuration to obtain a plurality of representative conformations of the probe in the aqueous solution; performing quantum chemical calculation on each representative conformation to obtain first system energy of each representative conformation in a stable state and second system energy and intensity parameters of each representative conformation in a lowest singlet excitation state; generating a comprehensive evaluation factor based on the first system energy, the second system energy and the intensity parameter; and sorting the luminescence properties of the probes based on the comprehensive evaluation factor, and screening out the probe with the optimal performance. According to the method provided by the invention, quantitative evaluation on the luminescence property of the near-infrared two-region molecular fluorescent probe is realized, and technical support is provided for optimal design and clinical application of the probe.

The invention discloses a high-frequency hydroxyl marking speed measurement device and method, and relates to the technical field of flow field non-contact measurement, and the device comprises an ultraviolet femtosecond laser, two shaping units, a first laser, a camera, a time schedule controller, and a computer. The ultraviolet femtosecond laser is used for outputting ultraviolet femtosecond laser, shaping the ultraviolet femtosecond laser into filamentous laser through the first shaping unit, and injecting the filamentous laser into a flow field measurement area to generate labeled molecules; the first laser is used for outputting first laser, shaping the first laser into sheet laser through the second shaping unit, and injecting the sheet laser into a flow field measurement area to generate labeled molecular fluorescence; the camera is used for collecting labeled molecule fluorescence images to obtain spatio-temporal data; the time sequence controller controls the time sequence of each component of the device; processing spatio-temporal data of labeled molecular fluorescence by a computer to obtain a flow field velocity; the ultraviolet femtosecond laser with high instantaneous energy density is selected as the write-in laser, the repetition frequency of each part is improved, and the problems that conventional hydroxyl marking speed measurement depends on the high-temperature condition of a to-be-measured flow field environment and the repetition frequency is low are solved.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention belongs to the technical field of fluorescent active substance development, and particularly relates to a cadmium (II)-based metal organic framework crystalline material as well as a preparation method and application thereof. The molecular formula of the cadmium (II)-based metal organic framework crystalline material is {[Cd3 (Br-BDC) 3 (BMIP) 2]. 2H2O} n, cadmium (II) ions serve as connecting nodes, BMIP and H2 (Br-BDC) ligands serve as bridging ligands to be in mutual bridging to form a three-dimensional space structure, Cd-O secondary structural units are contained in the structure, three Cd atoms form a trinuclear Cd-O cluster structure through the Cd-O secondary structural units, and the Cd (II)-based metal organic framework crystalline material has the structural formula shown in the description. The Cd1 atom and the Cd1 # atom are in mirror symmetry. The divalent cadmium ion fluorescence sensing material can be prepared by adopting a one-pot solvothermal reaction; the preparation method has the advantages of simple process, convenience in operation, high yield, good reproducibility and the like; the cadmium (II) fluorescent sensing material disclosed by the invention is relatively high in fluorescence emission spectrum intensity and can be applied to the fields of molecular fluorescent probes, metal ion detection and the like.

The invention relates to the technical field of immunoassay, in particular to a full-automatic single-molecule fluorescence immunoassay analyzer which comprises an analyzer body and a sample adding bin and further comprises a plurality of transfer pipes located in the sample adding bin. The knocking rods are located in the sample adding bin, the knocking rods are used for knocking and vibrating the bottom of the transfer pipe, when the full-automatic single-molecule fluorescence immunoassay analyzer is used, the existence of bubbles can interfere with reading of fluorescence signals, so that an analysis result is inaccurate, the bubbles are discharged through knocking and vibrating, and the accuracy of the analysis result is improved. According to the method, accurate reading of fluorescence signals can be ensured, so that the analysis accuracy is improved, the fluorescence signal intensity is optimized, bubbles can shield part of the fluorescence signals, the signal intensity is weakened, after the bubbles are discharged, the fluorescence signals can be more fully detected, the signal intensity is optimized, and the result is clearer.

The invention discloses a digital single-molecule enzyme multi-activity characteristic cross-scale heterogeneity dynamic evaluation method, which comprises the following steps: diluting a pre-combined enzyme-template-primer ternary complex to a single-molecule level so as to load the enzyme-template-primer ternary complex into a reaction chamber in a micropore array chip for rolling circle amplification, a molecular beacon is combined with an amplification product to release a fluorescence signal; monitoring and acquiring the fluorescence signal in real time to generate a time-resolved fluorescence intensity curve; extracting a slope parameter of the fluorescence intensity curve so as to obtain a catalytic synthesis rate through conversion calculation for evaluating enzyme activity; the distribution of catalytic synthesis rate values is fitted using an n-fold Gaussian mixture model to obtain a standard deviation for assessing population homogeneity and a coefficient of variation for assessing stability. According to the invention, cross-scale analysis of single-molecule resolution, multi-parameter dynamic association and environmental interference processing can be realized at the same time, and resolution limitation of a traditional analysis technology is broken through by integrating single-molecule fluorescence tracking and population statistics.

The invention discloses a compound, a kit and application thereof, and relates to the technical field of biomedicine. The compound comprises an electron acceptor aromatic unit, an optional electron donor aromatic unit and a shielding unit, and the shielding unit shields intermolecular interaction of the electron acceptor aromatic unit and / or the electron donor aromatic unit. Wherein the electron acceptor aromatic unit, the electron donor aromatic unit and the shielding unit are connected through covalent bonds. The compound can be used as a molecular near-infrared-II fluorophore, and has higher quantum yield and good biocompatibility. The compounds emit fluorescence in the range of 900-1400 nanometers, the intermolecular and intramolecular interaction of the conjugated skeleton is weakened, and the quantum yield is improved. The high quantum yield, biocompatibility and near-infrared II emission of the compound provide opportunities for in-vivo application of near-infrared II imaging by using molecular fluorophores.

The invention discloses a liquid-phase protein chip method for simultaneously detecting three kinds of porcine coronaviruses and a kit thereof, which comprises the following steps of: after activating and coupling microspheres, respectively coating antigen on porcine delta coronavirus N protein, porcine acute diarrhea syndrome coronavirus N protein and porcine epidemic diarrhea virus N protein by using the microspheres; matching the two fluorescent dyes according to different concentrations to form fluorescent coding microsphere combination arrays with different energy levels; the microspheres are analyzed one by one based on the flow principle, red laser recognizes microsphere codes, green laser reads fluorescence intensity of report molecules, computer analysis and processing are carried out, and fluorescence signals are converted into quantitative detection results; and determining the optimization of the detection conditions of the liquid-phase protein chip after the liquid-phase protein chip test. The antigens coated on the microspheres only specifically react with corresponding antibodies and are not combined with antibodies generated by other pathogens; the PDCoV, SADS-CoV and PEDV antibodies in the sample to be detected can be rapidly, effectively and accurately detected, and reference is provided for prevention of the porcine coronavirus.

The invention discloses a brain-derived Tau protein single-molecule fluorescence immunoassay detection kit and a use method, the kit provided by the invention is composed of a BD-Tau magnetic bead reagent coated with a capture antibody, a BD-Tau calibrator, a BD-Tau quality control product, a biotinylated BD-Tau Detector reagent for detecting the antibody, a BD-Tau SBG reagent, an RGP reagent and a BD-Tau sample diluent, the magnetic bead concentration of the BD-Tau magnetic bead reagent is 0.04 mg / mL-0. 08mg / mL of carboxyl magnetic beads, the carboxyl magnetic beads are coated with Tau J5.H3 coated antibodies, the detection antibody in the BD-Tau Detector reagent of the biotinylated detection antibody is Tau12, the concentration of the detection antibody is 0.3 mu g / mL-0. 8mu g / mL, and the concentration of SBG in the BD-Tau SBG reagent is 40 pM-60 pM; the kit disclosed by the invention can be used by adopting any one of a one-step method, a two-step method or a three-step method to detect the content of the BD-Tau protein in a plasma sample, the detection limit is 0.050 pg / mL, and the detection range is 0.050 pg / mL-150 pg / mL; according to the invention, the specificity, accuracy and reliability of determination of the Alzheimer's disease are improved, and the problems of early diagnosis and early screening of the Alzheimer's disease are solved.

The invention provides an integrated platform for exciting a molecular fluorescence signal, which comprises an input optical coupling structure, a first filtering structure, a light beam splitting tree structure, a filtering structure array, a fluorescence excitation structure array and an optical coupling structure array which are arranged in sequence, an integrated fluorescence excitation scheme is provided for detection of biological sample fluorescence signals. Compared with a traditional excitation scheme, the on-chip filter is introduced, wide-spectrum background light composed of spontaneous radiation fluorescence of laser or waveguide can be reduced, and therefore shot noise is reduced, and the signal-to-noise ratio and the signal background ratio can be better improved.

The invention belongs to the field of fluorescent sensors, and relates to an Ag-CP fluorescent probe material as well as a preparation method and application thereof. The structural formula of the fluorescent probe material is [Ag (HL) (L)] n, and an HL ligand is 4-(3, 5-dimethyl-1H-pyrazole-1-yl) benzoic acid. Ag-CP is prepared by the following method: dissolving HL and AgNO3 in H2O to obtain light brown turbid liquid, carrying out high-temperature reaction on the light brown turbid liquid, and cooling to obtain the Ag-CP. The Ag-CP material can identify tryptophan (Trp) molecules in an aqueous solution with high selectivity and high sensitivity, has good stability and anti-interference ability in fluorescence identification ability, and can be used as an excellent Trp molecule identification fluorescence sensor.

The invention belongs to the field of molecular fluorescent probes, and particularly discloses a near-infrared fluorescent probe of quinone oxidoreductase 1 (NAD (P) H: quinone oxidoreductase 1, NQO1) with an in-situ covalent anchoring function and a preparation method of the near-infrared fluorescent probe. According to the probe, dicyanoisophorone is used as a core fluorophore, an aldehyde group is used as an NQO1 covalent anchoring group, and 3-methyl-3-(2, 4, 5-trimethyl-3, 6-dioxocyclohex-1, 4-diene-1-yl) butyrate is used as a recognition group of NQO1. The invention also provides an application of the near-infrared fluorescent probe with the in-situ covalent anchoring NQO1. Compared with other probes, the probe is simple in synthesis method and suitable for industrial production, and has high sensitivity, high selectivity and good biocompatibility in NQO1 detection. The fluorescent probe disclosed by the invention can realize covalent labeling and in-situ imaging functions after target NQO1 recognition response. Long-time in-situ tracking and imaging can be carried out on tumor cells and tissues by monitoring the NQO1 level, and a simple and effective method is provided for accurate detection of tumor disease related protease.

The application belongs to the field of new application of functional proteins and mutants thereof, and discloses application of sugarcane ScCBS protein and key domain deletion mutants thereof. The nucleotide sequence of the sugarcane ScCBS protein is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The key domain deletion mutants of the ScCBS protein are ScCBS△motif1, ScCBS△motif3, ScCBS△motif4 and ScCBS△motif9, which respectively lack 28, 50, 49 and 29 amino acids. The application proves that the ScCBS protein can interact with SCSMV P1 and inhibit the RNA silencing suppressor activity by means of yeast two-hybrid, two-molecular fluorescence complementary experiment and agrobacterium transient expression system; the four mutants all lose the interaction and inhibition activity. Therefore, the ScCBS protein and the key domain deletion mutants thereof can be used for cultivating excellent SCSMV-resistant materials, and have wide application prospects in sugarcane disease-resistant molecular breeding.

The invention discloses application of a downstream transcription factor ONAC131 of an SL signal channel in improving the antiviral property of rice, and belongs to the technical field of biology. Experiments such as transcriptome sequencing screening, yeast two-hybridization and bimolecular fluorescence complementation verify that ONAC131 is an SL downstream response factor and can interact with MID1 to jointly regulate and control expression of RDR1 and RDR6 and activate an antiviral RNAi pathway. Overexpression of ONAC131 can enhance the resistance of rice to grass stunt virus (RGSV), and otherwise, deletion of ONAC131 can weaken the antiviral effect of rice. The gene supplements an SL downstream regulation framework, provides a new target for rice antiviral molecular breeding, and assists in breeding of new varieties of high-yield and high-resistance rice.

The invention discloses photochromic supramolecular fluorescent ink as well as a preparation method and application thereof in fluorescent falsification prevention. The photochromic supramolecular fluorescent ink comprises an aqueous solution of a photochromic organic compound and an aqueous solution of gamma-cyclodextrin for treating a substrate, the structure of the photochromic organic compound is as shown in formula I. The photochromic supramolecular fluorescent ink disclosed by the invention can realize high-precision and high-stability fluorescent pattern printing, and the unique fluorescence change characteristic of the photochromic supramolecular fluorescent ink provides extremely high safety and recognition for an anti-counterfeiting mark, so that the anti-counterfeiting mark is difficult to copy or tamper; the method can be widely applied to the fields of various anti-counterfeiting files, bills, product packaging and the like.

The utility model discloses a reaction device for preparing a molecular fluorescent probe, which comprises a support plate, an adjusting rod fixedly connected to the outer surface of the top of the support plate, a moving ring slidably connected to the outer surface of the adjusting rod, a funnel sleeved in a limiting ring, a connecting pipe fixedly connected to the bottom of the funnel, and a conical flask arranged below the connecting pipe. The conical flask is placed above the supporting plate; a positioning frame is fixedly connected to the upper surface of the fixing ring, a fixing block is slidably connected to the interior of the positioning frame, a positioning block is rotatably connected to the fixing block, a torsional spring is arranged between the positioning block and the fixing block, and a limiting plate is fixedly connected to the outer surface of the upper portion of the positioning block. According to the reaction device for preparing the molecular fluorescent probe, the positioning block is pressed on the outer surface of the filter paper, so that the position of the filter paper can be fixed, the filter paper cannot move under the driving of the glass rod when a user conducts drainage and filtration, and then liquid is prevented from moving into filtrate from the edge of the funnel; therefore, the purity of the interior of the filtrate is relatively high.

The invention provides a biomarker detection method which comprises the following steps: carrying out fluorescence labeling on a specific biomarker in a biological sample, and carrying out single-molecule fluorescence imaging on the biomarker to obtain multiple frames of original microscopic fluorescence images; and analyzing and processing the multiple frames of original microscopic fluorescence images so as to extract the super-resolution distribution characteristic information of the specific biomarker on the surface of the biological sample. The method comprises the following steps: processing an original microscopic fluorescence image to obtain positioning table data; rendering positioning points in the positioning table data to generate a super-resolution image; performing convolution operation, opening operation, convolution filtering and region segmentation on the super-resolution image to calculate the area of cells, the number of clusters and the area of a single cluster, determining the density of the clusters and the area ratio of the clusters by using the two, and calculating the closest distance between the clusters by using the center points of the clusters; and the accuracy of a biomarker detection result is greatly improved.

The application discloses a tryptamine detection method based on triple emission MOFs and a smart phone. A rare earth metal organic framework (AP-Eu) is used as a luminescent matrix, and a fluorescent guest molecule fluorescein-5-isothiocyanate (FITC) is post-modified to obtain a ratio fluorescent material with triple emission characteristics. Competitive absorption of ligands and tryptamine, pH response of FITC and hydrogen bonding are used to realize fluorescence signal regulation, and then high-sensitivity and high-selectivity fluorescence recognition of tryptamine is realized. The method is further applied to quantitative detection of tryptamine in beer samples, visualization detection of foam and on-site rapid detection assisted by a mobile phone APP. The application provides a safe and efficient tryptamine detection and fluorescence sensing method, and significantly improves detection accuracy and on-site applicability.

The invention discloses a supramolecular fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of nano material application. The supramolecular fluorescent probe is formed by compounding gold nanoclusters and a bovine serum albumin solution, and the concentration ratio of the gold nanoclusters to the bovine serum albumin solution is (1-2): (2-4). The invention further discloses a preparation method of the supramolecular fluorescent probe and application of the supramolecular fluorescent probe. A fluorescent sensing system of the supramolecular fluorescent probe based on the gold nanocluster material and the bovine serum albumin has excellent selectivity and sensitivity on cypermethrin pesticide, interference of other impurities can be effectively avoided, and response is fast.

The invention discloses a self-feedback intelligent electrochemical wastewater treatment platform and a construction method thereof. The platform modularly integrates an electrochemical water treatment unit, a terephthalic acid probe molecular fluorescence sensing unit, a signal acquisition data processing unit and an electro-hydraulic linkage online control unit. All the units work cooperatively, and the electrochemical water treatment unit generates strong oxidizing hydroxyl radical mineralized pollutants; the fluorescence sensing and data processing unit is used for converting. OH specificity into a fluorescence signal by utilizing probe molecules and mapping the fluorescence signal into. OH steady-state equivalent concentration in real time; and the online control unit implements a model prediction control strategy based on concentration feedback, dynamically adjusts the current density, the electrode potential and the wastewater flow, and realizes real-time adaptive matching of the interface mass transfer and the. OH generation rate. According to the invention, electrogenerated. OH on-line monitoring and dynamic closed-loop optimization of operation parameters are realized, deep mineralization, low energy consumption and long-term stable operation are realized, and the method is suitable for engineering and intelligent operation of electrochemical advanced oxidation treatment of industrial wastewater.

The utility model relates to the technical field of biological imaging, in particular to a three-dimensional optical imaging system. The imaging unit and the projector are installed in an inner cavity of a box body side by side, the LED light source and the object carrying unit are sequentially installed in the inner cavity of the box body along an imaging path of the imaging unit, the LED light source comprises at least two lamp caps distributed on the periphery of the imaging path in the circumferential direction, and irradiation light of the lamp caps obliquely points to the object carrying unit. And the irradiation areas of all the lamp caps on the object carrying unit are overlapped and all cover the imaging view field. According to the multi-modal imaging device, multi-modal imaging of biological optics can be achieved, the overall layout of the device is more reasonable, and better adaptability is achieved in the image acquisition process of molecular fluorescence imaging.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a single-molecule fluorescent probe based on a phenothiazine structure as well as a preparation method and application of the single-molecule fluorescent probe. The preparation method comprises the following steps: firstly introducing an alkyl chain to a nitrogen atom of a raw material to obtain the phenothiazine structure-based single-molecule fluorescent probe, then carrying out regioselective formylation on the raw material to obtain the phenothiazine structure-based single-molecule fluorescent probe, then treating the raw material by adopting aluminum trichloride to realize O-demethylation, and finally carrying out intramolecular cyclization reaction on the raw material and 1H-benzimidazole-2-acetonitrile to obtain the phenothiazine structure-based single-molecule fluorescent probe. The single-molecule fluorescent probe based on the phenothiazine structure prepared by the invention is compatible with orthogonal chemical reaction activity and maintains controllable electron transfer, so that different chemical events are converted into specific optical signals, and selective and mechanism specific fluorescent response to phosgene, copper ions and hypochlorite is realized.

The invention discloses a method for detecting the conformational change of an APO-Cascade protein. Through mutation of Cas8 protein amino acid N376 and Cas5 protein K169 in a Casade protein, the obtained Cas8 and Cas5 labeled by non-natural amino acids can maintain functions, and at the same time, the mutant type of the Cas5 / 8 protein can be subjected to fixed-point fluorescence labeling; according to the method, the Cas6 is used as a template, the N end of the Cas6 is further modified with biotin, and a formed Casade compound can be fixed on a biotin-PEG functionalized single-molecule slide, so that the conformation state of the Casade protein can be truly reflected by using single-molecule fluorescence resonance energy transfer, an effective means is provided for in-vitro research on the structure-function relationship of a system in CRISPR / Casade, and the method has a wide application prospect. And the method is of great significance in designing the Cascade protein with optimized properties and enabling the Cascade protein to play new functions.

The invention discloses an i-motif DNA (deoxyribonucleic acid) modified gold nano flare, a preparation method of the i-motif DNA modified gold nano flare and application of the i-motif DNA modified gold nano flare to detection of miRNA, and belongs to the technical field of nano biological materials and molecular diagnosis. The fluorescence probe is formed by assembling gold nanoparticles and an i-motif molecular fluorescence probe, the i-motif molecular fluorescent probe contains four sections of sequences rich in C basic groups and a target miRNA recognition chain, sulfydryl is marked at the 5'end, and fluorescent molecules are marked at the 3 'end. The probe is prepared by adopting a freeze thawing method, the labeling density of the probe is high, in a constructed detection system, the miR-21 detection limit is as low as 1.01 fM, and single-base mutants can be accurately distinguished; the technology does not need complex sample pretreatment and can be directly used for detecting cell total RNA or miRNA in a plasma sample, and the detection result is highly consistent with RT-qPCR; the problems of low marking efficiency and insufficient sensitivity in the prior art are effectively solved, and an innovative tool with high sensitivity, high specificity and clinical suitability is provided for tumor-associated miRNA molecular diagnosis.

The present invention provides an integrated platform for exciting a molecular fluorescence signal, a detection chip, and an optical packaging structure. The integrated platform for exciting the molecular fluorescence signal comprises, arranged in sequence: an input optical coupling structure (1), a first filtering structure (2), an optical beam-splitter tree structure (3), a filtering structure array (4), a fluorescence excitation structure array (5), and an optical coupling-out structure array (6). The detection chip is used for detecting molecular fluorescence and comprises, arranged from bottom to top in sequence: a fluorescence detection layer (103'), a fluorescence collection layer (102'), and a fluorescence excitation layer (101'). The fluorescence excitation layer (101') comprises, arranged in sequence: an input optical coupling structure (1011'), an optical beam-splitter tree structure (1013'), a fluorescence excitation structure array (1014'), and an optical coupling-out structure array (1015'). The fluorescence excitation layer is further provided with an optical feedback structure (1012'), wherein an input end of the optical feedback structure (1012') is connected to a first output end of one branch of the optical beam-splitter tree structure (1013'). The optical packaging structure comprises an optical fiber module (2') and the detection chip (1'). The integrated platform in the present invention incorporates on-chip filters, providing an integrated fluorescence excitation solution capable of suppressing broadband background light arising from spontaneous fluorescence of lasers or waveguides, thereby improving a signal-to-noise ratio. The detection chip and the optical fiber module in the optical packaging structure in the present invention employ a passive optical coupling scheme, thereby facilitating stability of fluorescence signals and reducing complexity of external device optical paths.

The invention relates to a molecular fluorography detection method combined with liquid chromatography, and belongs to the technical field of analysis and detection. The invention aims to solve the technical problems that the high sensitivity is difficult to maintain while the miniaturization of the existing liquid chromatogram fluorescence detector is realized, and a flexible and low-cost detection scheme is lacked. According to the technical scheme, the method is characterized in that after being subjected to liquid chromatography separation, a sample is conveyed to a silica gel coating preparation plate through a polyether-ether-ketone tube to form a detection area; exciting fluorescence by adopting a laser with a first narrow-pass optical filter; acquiring a series of fluorescence images by using an image acquisition unit with a second narrow-pass optical filter; and finally, extracting RGB (Red, Green and Blue) numerical values of a fixed pixel region through computer image processing software, and generating a chromatogram of which the fluorescence intensity changes along with time. The method is mainly used for rapid and high-sensitivity fluorescence detection in the fields of biomedical analysis, environmental monitoring, food safety and the like.

The present invention discloses a small-molecule fluorescent probe for CDK4 / 6, its preparation method, and applications, belonging to the field of biomedicine. The CDK4 / 6 small-molecule fluorescent probe provided by the present invention has a structure represented by Formula (I), a novel probe response group, and good selectivity. The preparation method also features mild reaction conditions, readily available and inexpensive raw materials, and simple operation and post-processing. The probe exhibits high affinity for the CDK4 / 6 protein and high sensitivity.

The application discloses a KRAS G12D A small-molecule fluorescent probe, a preparation method and application thereof, and a structure of the small-molecule fluorescent probe is shown as follows. An activity determination method based on the small-molecule fluorescent probe has the advantages of a simple test system, no need of a complex buffer system, small influence of time and solvent, etc., and can be used for screening of KRAS G12D inhibitors.

The present invention discloses a quinoline-based small-molecule fluorescent probe for hydrogen peroxide and its preparation method. This invention belongs to the field of fluorescent probe technology. The specific structural formula of the fluorescent probe is shown in Formula (I): #imgabs0# The present invention uses 2-methyl-7-hydroxyquinoline as the fluorescent group and 4-bromomethylphenylboronic acid pinacol ester as the recognition group. The prepared fluorescent probe exhibits high sensitivity and selectivity for H2O2 in solution. The preparation method of this fluorescent probe is simple, the raw materials are readily available, and the resulting product is a solid powder that is easy to store. It has broad application prospects and can be used in large-scale production.

The application discloses a new method for quantitatively analyzing interface nucleic acid hybridization reaction and application thereof, and comprises the following steps: S1, constructing a DNA tetrahedron interface; S2, imaging the DNA tetrahedron interface through a total internal reflection fluorescence microscope; S3, realizing real-time monitoring of a hybridization event of the DNA tetrahedron interface by adopting double-color co-localization and analyzing a fluorescence signal; and S4, analyzing hybridization kinetics on the DNA tetrahedron interface by recording changes of the fluorescence signal with time. The application uses the DNA tetrahedron interface for single-molecule fluorescence imaging, can realize real-time monitoring of a single nucleic acid molecule hybridization event, improve detection sensitivity and specificity, accurately analyze kinetic parameters of a hybridization reaction, help realize more accurate performance evaluation of a nucleic acid sensor, has important significance for design and optimization of the nucleic acid sensor, and thus plays a greater role in the fields of clinical diagnosis, environmental monitoring and food safety.