283 results about "Molecular fluorescence" patented technology

The invention provides a fluorescent probe represented by the formula (I), wherein R1 and R2 are independently selected from H or an aromatic vinyl group, and at least one of R1 and R2 is the aromatic vinyl group; R3 is selected from H, F, Cl, Br, OH, OCH3, N(CH3)2 or C1-C6 alkyl; and R4 is selected from C1-C6 alkyl. Compared with the prior art, because the fluorescent probe provided by the invention has a relatively large electronic conjugated system and plane, the intensity of the intramolecular charge transfer effect can affect the molecular fluorescence emission intensity; when the fluorescent probe and the G-quadruplex generate a specific action, the flexibility of intramolecular rotatable double bonds is restricted, the intramolecular charge transfer effect is enhanced, and the fluorescence is also enhanced significantly; moreover, the fluorescent probe provided by the invention has the advantages of low biotoxicity, phototoxicity and photobleaching property, good light stability, good water solubility and good cell membrane permeability.

The invention discloses a preparation method of molecularly imprinted polymeric microspheres for 2,4,6-trinitrophenol detection. The preparation method is characterized in that polynitrophenol derivatives-imprinted molecules generated through reaction between 2,4,6-trinitrophenol and N,N'-diisopropyl carbodiimide have luminous yellow fluorescent light, the fluorescent light of the imprinted molecules has open and close characteristics under an acid-base condition, the imprinted molecules located in an imprinting layer of the molecularly imprinted polymeric microspheres can be eluted, a hole structure which is complementary with the structure, the size and the functional groups of the imprinted molecules is formed in the imprinting layer, polymeric microspheres of which the imprinted molecules are eluted have specific recognition sites on target analyte molecules, target molecules enter the specific recognition sites, selective recognition on the target analyte molecules is realized, sensitivity detection of the target analyte molecules can be realized through opening and closing of the fluorescent light of the target molecules, the specific surface area of the molecularly imprintedmicrospheres is large, and space in which the recognition sites are formed is stable in structure, has more effective sites, and is capable of carrying out high-selective recognition and high-sensitive detection on the target molecules.

The invention discloses a Fe<3+> molecular fluorescence sensor based on Rhodamine B and a preparation method and application of the Fe<3+> molecular fluorescence sensor. Rhodamine B is taken as a precursor, and a target product N1-(benzene[d] thiazole-2-yl)-N4-(2-(3',6'-bi(diethylin)-3-carbonyl screw[iso-indoline-1,9'-xanthene]-2-yl)ethyl) maleimide is synthesized at two steps. Detection on Fe<3+> by using the target product shows that the target product has a very good detection effect on Fe<3+>; and meanwhile the raw materials of the Fe<3+> molecular fluorescence sensor are easy to obtain, synthesis steps are simple, after-treatment is convenient to implement, and large-scale production can be relatively easily achieved.

Provided is a method of producing a polymeric fluorescent substance wherein one or more monomers represented by the general formula (1) are polymerized in the presence of a zerovalent nickel complex,

X₁—Ar₁—X₂  (1)

wherein, Ar₁ represents a divalent group selected from the group consisting of arylene groups, divalent heterocyclic compound groups, and divalent or trivalent hetero atom-bonded arylene or divalent heterocyclic compound groups, and X₁ and X₂ represent leaving groups. By using the polymeric fluorescent substance, a high performance polymer LED can easily be obtained.

The invention discloses a substituted Rhodamine B acylthiosemicarbazide fluorescence probe compound and a preparation method and application thereof, and belongs to the technical field of synthesis of fluorescence probe compounds. According to the technical scheme, the substituted Rhodamine B acylthiosemicarbazide fluorescence probe compound has a chemical structural formula (please see the formula in the description), wherein R is Cl or OMe or CF3. The invention further discloses the preparation method of the substituted Rhodamine B acylthiosemicarbazide fluorescence probe compound and application of the substituted Rhodamine B acylthiosemicarbazide fluorescence probe compound serving as an organic molecular fluorescence probe for detecting heavy metal mercury ions. The fluorescence probe compound shows the good antijamming capability, can accurately detect a trace quantity of heavy metal mercury ions and is good in selectivity and sensitivity.

The invention discloses a method for fast optical tracking of a single molecule and a device, and relates to a technology for detecting single-molecule fluorescence spectroscopy and signal processing.The method uses the single-photon detection technology for detecting molecular fluorescence, analyzes the detected position with strongest fluorescence strength for determining the position of the single molecule, controls a nano-translation stage by feedback of a computer for tracking the position of the molecule and realizes the fast tracking of the motion trajectory of the molecule. The methodadopts the low-frequency sine wave modulation for triggering a laser signal of a single-molecule sample, thereby improving the signal-to-noise ratio. Compared with the prior art, the method adopts the single-photon detection technology, thereby being capable of determining and fast tracking the position of the single molecule in a relatively fine manner; the central position of the optoelectronicfeedback control nano-translation stage is changed along with the position of the single molecule, thereby being capable of reflecting the motion trajectory of the single molecule in a real-time manner; and the use of the modulation and demodulation technology can effectively suppresses the impacts of background noise on the detection of a fluorescence signal of the single molecule.

The invention relates to a measurement system for obtaining a single molecular spectrum and imaging under a shear field, characterized in that the measurement system includes: an excitation light source unit used as excitation light to irradiate a sample to be tested; a unit for applying a mechanical A shear application and rheological measurement unit for simultaneously measuring its macroscopic rheological properties; and an optical microscopic unit, the optical microscopic unit is used to introduce the excitation light emitted by the excitation light source unit into the waiting area under the shear field. The sample is measured, so that the dye molecules of the sample to be tested are excited to generate fluorescent signals, and the generated fluorescent signals are collected and respectively transmitted to a single-molecule fluorescence imaging unit, a single-molecule fluorescence emission spectrum measurement unit and/or a fluorescence correlation spectrum measurement unit . The invention can be widely applied to polyelectrolyte dilute solution, gel, interfacial diffusion, film imaging and other multi-charge condensed state systems, and explores the basic physical origin of its special properties.

The present invention relates to a method for analyzing the behavior and states of molecules present in an interface region, and an apparatus for performing the method.

The invention discloses a synthesis method of a spiropyrane small-molecule fluorescent probe with extreme acid/extreme alkaline switch response and an application of the spiropyrane small-molecule fluorescent probe. By virtue of the synthesis method, naphthalene nucleus spiropyrane with two carboxyl groups and a phenolic hydroxyl group is synthesized by carrying out modification on three loca of spiropyrane, wherein the naphthalene nucleus spiropyrane has the characteristic of reversible switch type response on pH values of extreme acid/extreme alkaline; when the pH value is smaller than 1.0, red fluorescence is represented; when the pH value is 1.0-12.0, fluorescence is represented; when the pH value is greater than 12.0, blue-green fluorescence is represented. The synthesized spiropyrane small-molecule fluorescent probe also has the characteristics of being high in optical, thermal and chemical stability and structural designability, and excellent in biocompatibility and cell labeling property; meanwhile, the synthesized spiropyrane small-molecule fluorescent probe is safe and low in low toxicity, and is capable of smoothly entering bacteria for bio-labeling, implementing the pH response of extreme acid/ extreme alkaline as same as that of solutions in organisms; furthermore, the synthesized spiropyrane small-molecule fluorescent probe can be used as a multifunctional cell fluorescence labeling dye and can be applied to scientific research and gene diagnosis in the field of biological medicines.

A method for generating same-beam dual-wavelength dual-pulse laser uses a tunable laser crystal suitable for flash-lamp pump to obtain Q-regulating pulse output, and can be used for pollution measuring radar, holographic molecular fluorescence diagnosis, satellite range finding, etc. A photoelectric switch device is used to make the laser oscillate sequentially in the sub-cavities of two channels during a pump pulse. As their oscillating wavelengthes are different, there is dual-wavelength dual-pulse output.

The invention discloses a tumor-targeted diagnosis and treatment integrated fluorescent probe which takes a tumor-targeted polypeptide sequence and an apoptosis enzyme specific recognition polypeptide sequence as a framework and comprises a fluorescence quenching molecular fluorescence pair and a photodynamic treatment photosensitizer. The diagnosis and treatment integrated fluorescent probe can achieve targeted treatment of tumors, and reduce toxic and side effects of the photodynamic treatment photosensitizer; a treatment result can be assessed in situ accurately in real time while the tumors are subjected to photodynamic treatment. The tumor-targeted diagnosis and treatment integrated fluorescent probe can also be used as a common fluorescent probe and used for screening of tumor treatment medicines and cell apoptosis imaging in a proportional fluorescence imaging manner. The tumor-targeted diagnosis and treatment integrated fluorescent probe can realize early detection of tumor treatment feedbacks and has great significance in promotion of accurate treatment of tumors and personal treatment.

The invention relates to a phenothiazinyl quinazoline fluorescence ion probe and application thereof. A fluorescence chemical sensor is based on a phenothiazinyl-substituted quinazoline derivative, and the structural general formula of the derivative is shown as a formula (1), wherein R1 is a hydrogen atom, alkyl which contains 1 to 10 carbon atoms, ether, azyl, aliphatic amide, imide, an ester group, an aryl substituent group or a hybrid aryl substituent group. Compared with other macromolecule fluorescence probe molecules, the fluorescence ion probe has the characteristics of simplicity in dissolution and synthesis and the like, and good response and identification effects on Cu<2+>, and also has a good identification effect on Hg<2+> in the presence of Cu<2+>, and generates different fluorescence responses to Hg<2+> and Cu<2+> under different conditions, multifunctionality on ion detection, and can also be designed into ion light introducing or fluorescence inhibit (INH) logic gate.

The invention discloses a preparation method of a high-molecular fluorescent microsphere. The method includes following steps: dispersing 1 part by weight of polystyrene microspheres in 0.1-1 ml of ultrapure water; adding dropwisely the polystyrene microsphere aqueous dispersion to 3-10 ml of a polydiallyldimethylamine hydrochloride solution with a concentration being 1.0-4.0 mg/ml with stirring; continuously stirring the solution for 1-4 h; performing centrifugation and removing a supernate; performing a washing process through ultrapure water; performing a centrifugation and collecting precipitate; vibratingly dispersing the precipitate in 1-2 ml of ultrapure water; adding dropwisely the polystyrene microsphere aqueous dispersion to 5-50 ml of a marked or an unmarked polysaccharide-type polyelectrolyte macromolecule solution with a concentration being 1.0-6.0 mg/ml with stirring; continuously stirring the solution for 1-4 h; performing centrifugation and removing a supernate; dispersing a precipitate in pure water; performing a centrifugation and collecting precipitate; and dispersing the precipitate in ultrapure water to obtain polysaccharide-modified fluorescent microsphere. The high-molecular fluorescent microsphere is 1-10 [mu]m in particle size, is 350-800 nm in fluorescent excitation wavelength, and has excellent biocompatibility and stability.

The invention relates to a fluorescence spectrum imaging technique, aiming at solving the problem of fuzzy single-molecule fluorescence imaging arising from the fluctuation in molecular fluorescent radiation and lower signal-to-noise ratio. The invention provides a method and device for improving the definition of single-molecule fluorescence imaging. The method comprises the following steps of: scanning and irradiating a single-molecule sample by using laser which is subjected to intensity modulation by an acousto-optic modulator; receiving fluorescent photons emitted by the single-molecule sample and converting the fluorescent photons into a standard logic electrical pulse output signal by using a single-photon detector; inputting the logic electrical pulse output signal to a lock-in amplifier, meanwhile, inputting a synchronous sine waveform modulated voltage signal to the lock-in amplifier; and demodulating the logic electrical pulse output signal to obtain a detected fluorescence spectrum signal and imaging the fluorescence spectrum signal. According to the invention, the intensity modulation to the single-molecule fluorescent photons is achieved by using an intensity modulated excitation light field, the quantum fluctuation in photon counting is eliminated by using analog signals demodulated by the lock-in amplifier, the signal-to-noise ratio is increased, and therefore, the high-definition single-molecule fluorescence imaging is achieved.

Provided is the design, synthesis and applications of molecular fluorophores for bioimaging in the short wavelength infrared window (1000-1700 nm). The molecular fluorophores compound comprise structures with electron accepting aromatic units, electron donating aromatic units, and an shielding units which shield the conjugated backbones from intermolecular interactions.

The invention relates to a fluorescence detection method for acetamiprid. The fluorescence detection method comprises the following steps: (S1) activating carbon quantum dots, equally dividing the carbon quantum dots into two parts, adding one part into a nucleic acid aptamer solution with a specific recognition effect to acetamiprid, adding the other part into a nucleic acid aptamer complementarychain solution, carrying out oscillation coupling, and mixing two solutions, so as to obtain carbon quantum dot mixed liquid; (S2) separating residual nucleic acid aptamers and complementary chains in the carbon quantum dot mixed liquid obtained in the step (S1) by virtue of an electrophoresis method, so as to obtain a complementary chain modified carbon quantum dot solution only containing the aptamers; and (S3) adding a to-be-tested acetamiprid sample into the carbon quantum dot liquid obtained in the step (S1), oscillating for reaction, and measuring the fluorescence intensity of the solution by virtue of a molecular fluorescence photometer. By modifying the carbon quantum dots with the nucleic acid aptamers with a specific recognition effect to acetamiprid and the complementary chainof the aptamers, a rapid and sensitive detection method for acetamiprid is established through the change of the fluorescence intensity of the carbon quantum dots before and after acetamiprid is added.

The invention discloses a tungstate upconversion luminescence temperature sensitive material doped with rare-earth elements Er and Yb and a preparation method thereof, and belongs to the technical field of a luminescence material. A general chemical formula of the luminescence material is MWO4: xEr, yYb, wherein x is more than or equal to 0.025mol% and less than or equal to 2.0mol%, x: y=1: 10 (molar ratio), and in the general formula, M is selected from one or more of Zn, Mg or Ba. The prepared luminescence material has good upconversion luminenscence performance, is high in luminous intensity, excellent in temperature sensitive performance, high in sensitivity and wide in application temperature area and has wide application prospect in the fields such as a fluorescent temperature sensor, a biological molecular fluorescence marker, infrared detection and anti-counterfeiting, three-dimensional display, solar battery and the like.

The invention relates to a rare-earth element-containing up-conversion luminescent temperature sensitive material as well as a preparation method and application thereof. The luminescent material has a general chemical formula of (A0.5Re0.5)TiO3, wherein A is selected from one or more of Rb, K, Na, Li and Ag, and Re is selected from one or more of Er, Yb, Pr, Eu, Ho, Tm, Tb and Dy. The sensitive material can be used for preparing the material provided by the invention, and the luminescent material prepared by the method provided by the invention has excellent up-conversion luminescent performance and temperature sensitivity, high luminescent intensity, good temperature sensitivity, a wide application temperature range, stable chemical performance and a controllable physical size, is easy to prepare, is relatively low in cost, and has a wide application prospect in the fields of fluorescent temperature sensors, short-wave emission solid lasers, biological molecular fluorescence labeling, infrared detection and anti-counterfeiting, three-dimensional display, photoelectric integrated devices, solar batteries and the like.

The invention discloses a fluorescent protein iRFP-based dimolecular fluorescent fragment complementary system, and applications. According to the fluorescent protein iRFP-based dimolecular fluorescent fragment complementary system, phytochrome fluorescent protein iRFP is taken as a template, and is divided into a non-fluorescent nitrogen terminal fragment iRN97 and a non-fluorescent carbon terminal fragment iRC98 at the amino acid 97-98 sites; when fusion expression of the two fragments with protein pairs with interactions is realized, the two non-fluorescent fragments are drawn to be close to generate iRFP fluorescence; fusion expression of iRN97 with human immunodeficiency virus integrase IN, and fusion expression of iRC98 with cell protein P75 are realized respectively, the interaction between IN and p75 is studied in living cells via fluorescent complementation of iRN97 with iRC98; when a drug is capable of inhibiting the protein-protein interaction, it is impossible for iRN97 and iRC98 to be drawn to be close, so that fluorescence recovery is inhibited. The fluorescent protein iRFP-based dimolecular fluorescent fragment complementation system is a simple, effective, and convenient evaluation system of drugs used for inhibiting protein interactions.

The invention provides a hemicyanine borate molecular fluorescence probe, and a preparation method and application thereof. The probe is 2-(4-borophenylvinyl)-N-alkyl indoline borate. The synthesis method of the probe comprises the following steps: 1) mixing iodonium salt and 4-formacyl borophenylic acid used as raw materials according to a mol ratio of 1:1, and refluxing in ethanol for 6 hours; 2) adding KBF4, refluxing for 2 hours, and performing anion exchange; and 3) recrystallizing with absolute ethanol to obtain the pure product. According to the invention, the detection of the prepared hemicyanine borate molecular fluorescence probe on hypochlorite in tap water indicates very high fluorescence enhancement effect; the hemicyanine borate molecular fluorescence probe can be used for the detection of hypochlorite in environmental samples, tap water and chemical simulation biosystems, the analysis detection and fluorescent imaging detection of hypochlorite in biological living cells and living tissues and the detection of hypochlorite in pathological tissues in clinical medicine; and the hemicyanine borate molecular fluorescence probe has favorable effects of high response speed, high selectivity, high sensitivity, high practicality and the like.

The invention discloses rare earth element-doped bismuth layer-structured multifunctional materials and a preparation method thereof. The general chemical formula of the multifunctional materials is Bi(3-x-y-z)ErxYbyRzTi(1.5-0.5x1)W(0.5-0.5x1-x2)Mx1Mox2Ox3, wherein x is more than 0 and not more than 40 mol%, y is not less than 0, z is not less than 0 and not more than 40 mol%, x1 and x2 are more than 0 and not more than 30 mol%, x3 is not less than 8 and not more than 10, R is one or more of La, Li, Ce, Pr and Y, and M is one or more of Nb, Ta, Zr, Hf, Cr and Mn. The multifunctional materials prepared in the invention have the advantages of good ferroelectric/piezoelectric properties, high Curie point, high high-temperature thermal stability, high chemical durability, excellent up-conversion luminescence performance, excellent optical temperature sensing performance, high sensitivity and wide application temperature range, and has wide application prospects in the fields of optical temperature sensor, bio-molecular fluorescence labeling, infrared detection and anti-fake, luminescence displays and photoelectric integration.

The invention discloses a bowknot nanometer antenna device based on vanadium dioxide phase change dynamic adjustment. The bowknot nanometer antenna device comprises a vanadium dioxide film formed on asubstrate; a dielectric film formed on the vanadium dioxide film; and a metal antenna layer consisting of bowknot metal units which are periodically arranged on the dielectric film; each bowknot metal unit is composed of two structures which are symmetrical in the mirror direction. Furthermore, the invention also discloses a preparation method of the bowknot nanometer antenna device and a methodfor adjusting the resonant wavelength of the nano antenna. According to the invention, the resonance wavelength can be dynamically regulated and controlled in a near-infrared large wavelength range, and the bowknot nanometer antenna device can be applied to the fields of dynamically adjustable higher harmonics, dynamically adjustable molecular fluorescence, dynamically adjustable Raman scattering,dynamically adjustable nano laser and the like.