Molecular fluorescent probe for detecting hypochlorite and preparation and application thereof

A technology for the detection of hypochlorous acid and fluorescent probes, applied in the directions of fluorescence/phosphorescence, material excitation analysis, chemical instruments and methods, etc., can solve problems such as poor specificity, probe working conditions requiring strong alkalinity, etc., and achieve low cost , The preparation method is simple and easy to implement, and the pH value range is wide.

Inactive Publication Date: 2013-03-20
TIANJIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

Although the newly developed dihydrofluorescein and dihydrorhodamine have the function of recognizing such substances, they have cross-response with other related oxidants, and the specificity of selection is poor
Although the hydrazine derivative of N-benzoylrhodamine B can selectively detect hypochlorite, the working conditions of the probe require strong basicity

Method used

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  • Molecular fluorescent probe for detecting hypochlorite and preparation and application thereof
  • Molecular fluorescent probe for detecting hypochlorite and preparation and application thereof

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Embodiment

[0020] A preparation of a molecular fluorescent probe for detecting hypochlorite, the molecular fluorescent probe is 2-(4-boronic acid phenylvinyl)-N-ethylquinoline borate, the steps are as follows:

[0021] 1) In a nitrogen atmosphere, heat 602 mg (2 mmol) of N-ethyl-2-methylquinoline iodonium salt and 301 mg (2 mmol) of 4-formylphenylboronic acid in 25 ml of solvent absolute ethanol in the dark Reflux reaction, heating temperature is oil bath temperature 100°C, heating and reflux time is 6 hours, and then cooled to 22°C;

[0022] 2) Add 430 mg potassium tetrafluoroborate (KBF 4 ) and stirred for 2 hours, after anion exchange, the crude product was obtained;

[0023] 3) Filter the above crude product to remove inorganic salts, evaporate the solvent from the liquid phase under reduced pressure and concentrate to 5 ml, recrystallize the solid product with 5 ml of absolute ethanol, and obtain 423 mg of a yellow solid product after drying. 2-(4-Boronicophenylvinyl)-N-ethylquino...

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Abstract

The invention relates to a molecular fluorescent probe for detecting hypochlorite. The probe is tetrafluoborate of 2-)4-boronic phenyl vinyl)-N-alkyl quinoline. The preparation method comprises the following steps: 1) mixing iodine onium salt and 4-formyl borophenylic acid according to the molar ratio of 1:1, and counter-flowing for 6 hours in ethanol; 2) then adding KBF4 to counter-flow for 2 hours for anion exchange; and 3) using a purified product recrystallized by absolute ethyl alcohol. The molecular fluorescent probe provided by the invention has the advantages that the molecular fluorescent probe aiming at strong fluorescence enhancement effect of detection of hypochlorite in tap water can be applied to detecting hypochlorite in an environmental sample, tap water and chemical simulative biosystem, analysis and detection and fluorescence imaging detection of biological living cells and hypochlorite in living tissues as well as detection of hypochlorite in disease tissues in clinical medicine. The molecular fluorescent probe for detecting hypochlorite provided by the invention has the good effects of fast response speed, high selectivity, high sensitivity and strong practicality and the like.

Description

【Technical field】 [0001] The invention relates to the synthesis and detection method of molecular probes in fluorescence sensing and detection technology, in particular to a molecular fluorescent probe for detecting hypochlorite and its preparation and application. 【Background technique】 [0002] Due to the rapid development of traditional industrial technology, mineral development and electronic technology, various polluting ions and gases generated and discharged during its production and processing are more and more harmful to the natural ecological environment, and at the same time, they are more harmful to human health. bigger and bigger. As a result, many serious ecological and environmental problems have arisen, and directly lead to serious social security problems such as blood lead and food pollution. Therefore, it is particularly important to develop highly selective and sensitive chemical molecular sensors for the monitoring and detection of these pollutants. Fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F5/02G01N21/64
Inventor 曾宪顺王庆刘畅
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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